Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,001,9110GA45.8% 51.7 / 37.5 59intergenic (‑122/+199)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
Reads supporting (aligned to +/- strand):  ref base G (25/7);  new base A (0/27);  total (25/34)
Fisher's exact test for biased strand distribution p-value = 1.14e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAT  >  NC_000913/2001763‑2002061
                                                                                                                                                     |                                                                                                                                                      
acCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGa                                                                                                                                                       >  1:329742/1‑150 (MQ=255)
     cAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTGATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGaaaaaaa                                                                                                                                                 <  2:499923/151‑1 (MQ=255)
        cGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAccc                                                                                                                                              <  2:374929/151‑1 (MQ=255)
         ggTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAcccc                                                                                                                                             >  2:785786/1‑151 (MQ=255)
            ataGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGCTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCccgcc                                                                                                                                          >  2:689160/1‑151 (MQ=255)
              agagTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCgg                                                                                                                                        <  2:593477/151‑1 (MQ=255)
               gagTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCgg                                                                                                                                        >  1:750814/1‑150 (MQ=255)
                     aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCggg                                                                                                                                 >  1:469308/1‑151 (MQ=255)
                        cACGATAAACGGCCCTGCGTTATATGAGTTATCGGCATGATTTCCCGTTTCTGCAGGGTTTTTAATCGGACGTTTAGTGGGTGAAATGAGGGTTTATTGGGGGGTTAAAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGaa                                                                                                                              <  1:497153/151‑1 (MQ=255)
                        cACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCGGCAGGTTTTTTAATCGGACGATTGGTGGGTAAAAGGAGGGGTTATTTGGGGTTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCgggg                                                                                                                                <  1:166267/149‑1 (MQ=255)
                            aaaaaCAGCCCTGCTTTATATGAGTTATCGGAATGATTACCCTTTTCTGCAGGTTTTTTAATCGGACGTTTAGTGGGTGAAAGGAGGGTTTTTTGGGGGTTTACAGGTAAATCCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCa                                                                                                                           <  1:308118/148‑1 (MQ=255)
                              aaaCAGCCTTGTTTTAAAGGGTTTTTCGGATTGTTTTTCCTTTTCTGCGGGTTTTTTAATCGGACGTTTAGTGGGTGAAAGGGGGGTTTTTTGGGGGGTTACAGTAAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGc                                                                                                                            <  1:730720/147‑1 (MQ=255)
                               aaCAGCCCTGCGTTATATGAGTTATCGGCATGATTACCCGTTTCTGCGGGTTTTTTAATCGGACGATTAGGGGGTGAAAGGAGGGTTTTTTTGGGGGTTAAAGTAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGtt                                                                                                                       <  2:660591/151‑1 (MQ=255)
                                    cccTGCTTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGTTTTTTAATCGGACGATTAGGGGGTGAAATGAGGGTTTATTTGGGGGTTACAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGa                                                                                                                  <  1:90914/151‑1 (MQ=255)
                                    cccTGCGTTATATGATTTATCGGAATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGGGGGTAAAATGAGGGTTTATTGGGGGTTTACGGGTAAATCCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGc                                                                                                                     <  1:92993/148‑1 (MQ=255)
                                       tGCGTTATATGAGTTATCGGCATGATTATCCTTTTCTGAGGGGTTTTGAATCGGAAGAGTTGTGGGTAAAATGAGGGTTTATGTGGGGGTCACGGGAAATTCCCAGGCAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTg                                                                                                                   <  1:25520/147‑1 (MQ=255)
                                           ttATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                           >  1:444682/1‑151 (MQ=255)
                                              tatGAGTTTGCGGAATGATTATCCGTTTCGGCGGGTTTTTTAATCGGACGTTTAGTGGGTAAAAGGGGGGTTTTTTTGGGGGTTACAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgc                                                                                                          <  1:903672/149‑1 (MQ=255)
                                                 gatttAGCGGCTTGTTTTTCCGTTTCTGCAGGTTTTTTAACCGGACGATTGGTGGGTAAAATGGGGGTTTTTTTGGGGTTTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                           <  1:896509/142‑1 (MQ=255)
                                                 gAGTTCTCGGCATGTTTACCCTTTTCTGCAGGTTTTTTAATCGGACGATTAGGGGGTAAAAGGGGGGTTTTTTTGGGGGTAACAGGAAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCtt                                                                                                      <  2:543271/150‑1 (MQ=255)
                                                  aGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                     >  2:482555/1‑150 (MQ=255)
                                                    ttATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                                   >  2:51728/1‑150 (MQ=255)
                                                    ttAGCGGCATGATTATCCTTTTCTGCAGGTTTTTTAATGGGACTTTTAGGGGGTAAAATGGGGGTTTTTTTGGGGGTTACAGATAATTCCCAGGAAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                     <  2:895945/148‑1 (MQ=255)
                                                     tttCGGCTTGATTTTCCTTTTCTGAGGGTTTTTTAATCGGAGGTTTTGGGGGGAAAAATGGGGGTTTTTTGGGGGTTAAAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCt                                                                                                       <  1:851595/143‑1 (MQ=255)
                                                                 tccctgTTTCTGCAGGGTTTTAAATCGGACGATTAGGGGGGGAAATGAGGGTTTATTTGGGGGTTACAGGTAATTTCAAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcg                                                                                      <  2:789164/145‑1 (MQ=255)
                                                                       tttCTGCGGGTTTTTTAATCGGACGTTTGGTGGGTAAAAGGGGGGTTTTTTTGGGGGTTACAGGAAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggcg                                                                                   <  2:393508/147‑1 (MQ=255)
                                                                        ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAAc                                                                              >  1:254808/1‑151 (MQ=255)
                                                                           tGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGcc                                                                           >  2:903510/1‑151 (MQ=255)
                                                                             gaGGGTTTTTAAAGCGAACGATAGGTGGAAAAAAGGAGGGGTTGTTTGGGGGTACTAGAAAAATCCCAGAAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAAc                                                                              <  1:689256/146‑1 (MQ=255)
                                                                             cAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                         >  2:897442/1‑151 (MQ=255)
                                                                              aGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                         >  2:330030/1‑150 (MQ=255)
                                                                               gggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTa                                                                        >  2:411025/1‑150 (MQ=255)
                                                                               gggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                       >  1:533905/1‑151 (MQ=255)
                                                                               gggTTTTTAATCGGACGATGAGGGGGAAAAAGGGGGGGTTTTTTGGGGGTAACAGGAAAATTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                         <  2:828434/149‑1 (MQ=255)
                                                                                  tttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                    >  2:94227/1‑151 (MQ=255)
                                                                                  tttttAACGGGACGATTGGGGGGTAAAAGGGGGGGTTTTTTGGGGGTTACAGGTAATTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                      <  2:351823/149‑1 (MQ=255)
                                                                                    tttAAGCGGACGTTTGGGGGGTAAAAGGGGGGTTTTTTTGGGGGTTAAAGTTAAATCCCGGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                   <  2:403405/150‑1 (MQ=25)
                                                                                        aaTCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                >  1:926349/1‑150 (MQ=255)
                                                                                          tCGGAGGATTGGTGGGTAAAAGGAGGGGTTATTGGGGGTTTAAGGGTAAATCCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                              <  1:593525/150‑1 (MQ=37)
                                                                                          ccGGACGATTAGGGGGTAAAATGGGGGTTTTTTTGGGGTTTAAAGGAAATTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa                                                             <  2:539486/150‑1 (MQ=25)
                                                                                                gATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtg                                                       >  1:105149/1‑151 (MQ=255)
                                                                                                 aTTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtg                                                       >  2:811923/1‑150 (MQ=255)
                                                                                                  ttggggggTAAAAGGGGGGTTTTTTTGGGGGTTACAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt                                                        <  2:122933/143‑1 (MQ=25)
                                                                                                   tAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                                    >  2:315484/1‑151 (MQ=255)
                                                                                                       gggTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAt                                                <  1:643897/151‑1 (MQ=255)
                                                                                                       gggTGAAAGGAGGGTTTTTTTGGGGTTTAAAGTTAAATCCCAGACAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAt                                                <  1:240158/151‑1 (MQ=37)
                                                                                                       gggTAAAATGAGGTGTTATTGGGGGTTTACAGGTAATTTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCa                                                   <  1:868346/148‑1 (MQ=38)
                                                                                                          tGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTc                                              >  2:466912/1‑150 (MQ=255)
                                                                                                                ggggggTTTTTTGGGGTTTAAAGTAAAATTCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaaa                                        <  2:837228/148‑1 (MQ=25)
                                                                                                                 aGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAtt                                      >  1:490507/1‑151 (MQ=255)
                                                                                                                  gggtTTTTTTGGGGTTTACAGGAAAATTCCAGAAAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAtt                                      <  2:585858/150‑1 (MQ=37)
                                                                                                                         tttGGGGTTTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAt                                 <  1:118141/148‑1 (MQ=255)
                                                                                                                         tttGGGGGTTACAGTTAAATTCCAGGCAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                               <  1:660764/150‑1 (MQ=255)
                                                                                                                           tGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCaa                             >  2:428689/1‑150 (MQ=255)
                                                                                                                               ggTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                         >  1:686513/1‑150 (MQ=255)
                                                                                                                                  tACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg                     >  2:31206/1‑151 (MQ=255)
                                                                                                                                   aCAGGTAAATCCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg                      <  1:409450/149‑1 (MQ=255)
                                                                                                                                                aGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgc         >  2:740113/1‑149 (MQ=255)
                                                                                                                                                 ggaaGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgca        <  2:533819/146‑1 (MQ=255)
                                                                                                                                                     gAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAt  >  1:22915/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAT  >  NC_000913/2001763‑2002061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: