Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4860CA46.3% 51.4 / 24.1 41intergenic (+291/‑165)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base C (18/4);  new base A (0/19);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 5.49e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAT  >  NC_000913/29337‑29635
                                                                                                                                                         |                                                                                                                                                     
ttttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCcac                                                                                                                                                       <  1:247720/150‑1 (MQ=255)
   tgttgtTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACaaa                                                                                                                                                    >  1:526563/1‑150 (MQ=255)
         tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAataata                                                                                                                                              <  2:916224/150‑1 (MQ=255)
            aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                           >  2:829500/1‑150 (MQ=255)
             aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaa                                                                                                                                          >  2:54145/1‑150 (MQ=255)
               gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaaaa                                                                                                                                       <  2:769995/151‑1 (MQ=255)
                tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCACTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaaa                                                                                                                                        <  2:223419/149‑1 (MQ=255)
                        gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                              >  1:143805/1‑151 (MQ=255)
                         aCCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAtt                                                                                                                             >  1:618301/1‑151 (MQ=255)
                            aTTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                            >  1:725362/1‑149 (MQ=255)
                              ttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGtt                                                                                                                        >  2:503657/1‑151 (MQ=255)
                                  tCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACt                                                                                                                    >  1:249062/1‑151 (MQ=255)
                                     aCTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                 >  1:55884/1‑151 (MQ=255)
                                       ttttttCTGCTCTTTTTTTTTTCAGGAATTCTTTTTGCTGCGAAAGCTTTTTCAAAAAAGGGTAAGATGTTTTTTTTGTCGTTTAATGCCTGAAAAACTTGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                  <  1:559450/148‑1 (MQ=255)
                                        ttttcctgttttttttttttTCATACATTCTTCTTCCTGCACAAGCTTTTCCAAAACCAGTTTAAATAATTTTTTTCTCCTTAATTCCATAAAAAACATGCATGAGACAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACtttt                                                                                                                 <  2:720975/136‑1 (MQ=255)
                                                     ttttATTTCATCAATTTTTCTGGCTGCGAAGGCGTTTCCAAAAACAGTTTAAATGATTTTTTTGGCCTTTAAGGCCTTAAAAACGTGCGTGACCCAAAAAAAAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                  <  1:655152/150‑1 (MQ=255)
                                                      tttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                  >  1:422777/1‑149 (MQ=255)
                                                                gCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                      >  1:297775/1‑151 (MQ=255)
                                                                  aaTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGtt                                                                                    >  2:624729/1‑151 (MQ=255)
                                                                                  atgCGTTTACCGGAACAGTTTAGATTTTCTTTTTGTCCCTAATTGTTGTAAAAACGTGAATGAGCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                     <  1:801690/148‑1 (MQ=255)
                                                                                  aaGCTTTTCCAAAAAAAGGTAAGATGATTTTTTGGTCGTTTAATGCCTGAAAAACATGCAGGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCa                                                                               <  1:451287/140‑1 (MQ=255)
                                                                                        tttCCAAGAAGAGGTTAGAGGTTTTTTTTGCCGTTTAGTGCTGTAAAAACATTCAGGAGCCAAAAAATAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                 <  1:218764/149‑1 (MQ=255)
                                                                                               aaaagagtttAGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCAGGAGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                              <  1:297976/138‑1 (MQ=255)
                                                                                                       ttaattttttttttttTCGTTTAATGCCTAAAAACCATCAAGGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                  <  2:866933/140‑1 (MQ=255)
                                                                                                       taaGATGATCTTTTTGTCCTTTATCGCCGAAAAATCAGGAATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                      <  2:754482/144‑1 (MQ=255)
                                                                                                        taggtgtttttttttTCTCTTAATGCCTGAAAAACATGCAGGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGa                                                 <  1:712/141‑1 (MQ=255)
                                                                                                          gatgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                              >  1:438249/1‑151 (MQ=255)
                                                                                                            ttatttttttGTCTTTTAATCCCTAAAAAACATGCATGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                             <  2:15704/145‑1 (MQ=255)
                                                                                                            tgatTTTTTTGTCGCTTAATGCCTAAAAAACATGCATGAGCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                             <  1:169226/150‑1 (MQ=255)
                                                                                                                 tttttGTCCCTTAATCCCTATAAAACATGCATGAACCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                         <  2:313544/149‑1 (MQ=255)
                                                                                                                  ttttGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                      >  2:150094/1‑151 (MQ=255)
                                                                                                                   ttttacGCTTAGTCCTTGAAAAACATGCATGACCAAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                      <  2:7393/145‑1 (MQ=255)
                                                                                                                        cgttaaaTGCCTGAAAAACATGCAGGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                 <  1:546055/145‑1 (MQ=255)
                                                                                                                         gCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                >  2:738375/1‑150 (MQ=255)
                                                                                                                           ttAATGCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                              <  1:129985/150‑1 (MQ=255)
                                                                                                                               aatccttgaaaaaCATGAATGACCAAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                           <  1:487895/142‑1 (MQ=255)
                                                                                                                                 gcttGTAAAACATGCAAGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGTCTTTTAACGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCTTTGATTTACGTCTTCATTGTGAATTa                           >  2:824046/4‑148 (MQ=255)
                                                                                                                                             atgcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa               >  2:451605/1‑149 (MQ=255)
                                                                                                                                                catgACCAAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt            <  2:630191/149‑1 (MQ=255)
                                                                                                                                                caggAGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga          <  1:49764/148‑1 (MQ=255)
                                                                                                                                                caggAGCCAAAAAATAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAGagtg           <  1:424331/147‑1 (MQ=255)
                                                                                                                                                      ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat    >  2:906890/1‑151 (MQ=255)
                                                                                                                                                        acaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat  >  2:111517/1‑151 (MQ=255)
                                                                                                                                                         |                                                                                                                                                     
TTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAT  >  NC_000913/29337‑29635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: