Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4960TA51.0% 40.0 / 80.7 49intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (20/4);  new base A (0/25);  total (20/29)
Fisher's exact test for biased strand distribution p-value = 3.76e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-09

AATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29349‑29642
                                                                                                                                                       |                                                                                                                                                  
aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                                  >  2:829500/1‑150 (MQ=255)
 aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATaaa                                                                                                                                                 >  2:54145/1‑150 (MQ=255)
   gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATaaaaaa                                                                                                                                              <  2:769995/151‑1 (MQ=255)
    tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCACTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATaaaaa                                                                                                                                               <  2:223419/149‑1 (MQ=255)
            gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                                     >  1:143805/1‑151 (MQ=255)
             aCCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAtt                                                                                                                                    >  1:618301/1‑151 (MQ=255)
                aTTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                                   >  1:725362/1‑149 (MQ=255)
                  ttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGtt                                                                                                                               >  2:503657/1‑151 (MQ=255)
                      tCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACt                                                                                                                           >  1:249062/1‑151 (MQ=255)
                         aCTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                        >  1:55884/1‑151 (MQ=255)
                           ttttttCTGCTCTTTTTTTTTTCAGGAATTCTTTTTGCTGCGAAAGCTTTTTCA‑AAAA‑AGGGTAAGATGTTTTTTTTGTCGTTTAATGCC‑TGA‑AAAACTTGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                         <  1:559450/148‑1 (MQ=255)
                            ttttcctgttttttttttttTCATACATTCTTCTTCCTGCACAAGC‑TTTTCC‑AAAACCAGTTTAAATAATTTTTTTCTC‑CTTAATTCCATAA‑AAAACATGCATGAGACAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACtttt                                                                                                                        <  2:720975/136‑1 (MQ=255)
                                         ttttATTTCATCAATTTTTCTGGCTGCGAAGGCGTTTCCA‑AAAA‑CAGTTTAAATGATTTTTTTGGCCTTTAAGGCC‑TTA‑AAAACGTGCGTGACCCAAAAAAAAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         <  1:655152/150‑1 (MQ=255)
                                          tttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         >  1:422777/1‑149 (MQ=255)
                                                    gCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                             >  1:297775/1‑151 (MQ=255)
                                                      aaTCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGtt                                                                                           >  2:624729/1‑151 (MQ=255)
                                                                      atgCGTTTACC‑GGAA‑CAGTTTAGATTTTCTTTTTGTC‑CCTAATTGT‑TGTAAAAACGTGAATGAGCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                            <  1:801690/148‑1 (MQ=255)
                                                                      aaGCTTTTCCA‑AAAA‑AAGGTAAGATGATTTTTTGGTCGTTTAATGCC‑TGA‑AAAACATGCAGGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCa                                                                                      <  1:451287/140‑1 (MQ=255)
                                                                            tttCCAAGAA‑GAGGTTAGAGGTTTTTTTTGCCGTTTAGTG‑C‑TGTAAAAACATTCAGGAGCCAAAAAATAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                        <  1:218764/149‑1 (MQ=255)
                                                                                   aaaagagtttAGATGATTTTTTTGTCGCTTAATGCC‑TGA‑AAAACATGCAGGAGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                     <  1:297976/138‑1 (MQ=255)
                                                                                           ttaattttttttttttTCGTTTAATGCC‑TAA‑AAACCATCAAGGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                         <  2:866933/140‑1 (MQ=255)
                                                                                           taaGATGATCTTTTTGTCCTTTATCGCC‑GAA‑AAATCAGGAATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                             <  2:754482/144‑1 (MQ=255)
                                                                                            taggtgtttttttttTCTCTTAATGCC‑TGA‑AAAACATGCAGGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGa                                                        <  1:712/141‑1 (MQ=255)
                                                                                              gatgatCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                                     >  1:438249/1‑151 (MQ=255)
                                                                                                ttatttttttGTCTTTTAATCCC‑TAA‑AAAACATGCATGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                                    <  2:15704/145‑1 (MQ=255)
                                                                                                tgatTTTTTTGTCGCTTAATGCC‑TAA‑AAAACATGCATGAGCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                                    <  1:169226/150‑1 (MQ=255)
                                                                                                     tttttGTCCCTTAATCCC‑TAT‑AAAACATGCATGAACCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                <  2:313544/149‑1 (MQ=255)
                                                                                                      ttttGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                             >  2:150094/1‑151 (MQ=255)
                                                                                                       ttttacGCTTAGTCCT‑TGA‑AAAACATGCATGACCAAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                             <  2:7393/145‑1 (MQ=255)
                                                                                                            cgttaaaTGCC‑TGA‑AAAACATGCAGGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                        <  1:546055/145‑1 (MQ=255)
                                                                                                             gCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                       >  2:738375/1‑150 (MQ=255)
                                                                                                               ttAATGCC‑TGA‑AAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                                     <  1:129985/150‑1 (MQ=255)
                                                                                                                   aatccttgaaaaaCATGAATGACCAAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                                  <  1:487895/142‑1 (MQ=255)
                                                                                                                     gcttGT‑AAAACATGCAAGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGTCTTTTAACGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCTTTGATTTACGTCTTCATTGTGAATTa                                  >  2:824046/4‑148 (MQ=255)
                                                                                                                                 atgcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                      >  2:451605/1‑149 (MQ=255)
                                                                                                                                    catgACCAAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                   <  2:630191/149‑1 (MQ=255)
                                                                                                                                    caggAGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga                 <  1:49764/148‑1 (MQ=255)
                                                                                                                                    caggAGCCAAAAAATAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAGagtg                  <  1:424331/147‑1 (MQ=255)
                                                                                                                                          ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat           >  2:906890/1‑151 (MQ=255)
                                                                                                                                          caaaaaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg              <  1:288450/144‑1 (MQ=255)
                                                                                                                                          caaaaaaaTATAAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg              <  2:75506/144‑1 (MQ=255)
                                                                                                                                          caaaaaaaTAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat           <  1:779653/147‑1 (MQ=255)
                                                                                                                                           caaaaaaaaATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                  <  1:882249/140‑1 (MQ=255)
                                                                                                                                            acaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat         >  2:111517/1‑151 (MQ=255)
                                                                                                                                              aaaaTAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc       >  2:934097/1‑151 (MQ=255)
                                                                                                                                              aaaaTAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt        <  1:54149/150‑1 (MQ=255)
                                                                                                                                               aaaTAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct      <  1:708636/151‑1 (MQ=255)
                                                                                                                                                   aataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg  >  1:211182/1‑151 (MQ=255)
                                                                                                                                                   aataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTg   <  1:311390/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                  
AATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAA‑CAGGTTAGATGATCTTTTTGTCGCTTAATGCC‑TGT‑AAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29349‑29642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑