Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,350,8530AC43.9% 93.5 / 14.0 57S725A (TCA→GCA) arcBaerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base A (31/1);  new base C (0/25);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 2.62e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACA  >  NC_000913/3350707‑3350998
                                                                                                                                                    |                                                                                                                                                 
tGCCACACACGCTTACAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcccc                                                                                                                                               >  2:542327/1‑151 (MQ=255)
 gCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcccc                                                                                                                                               >  2:315587/1‑150 (MQ=255)
   cacccacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccgcc                                                                                                                                            >  2:290810/1‑151 (MQ=255)
                   aCTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTAt                                                                                                                            >  1:734887/1‑151 (MQ=255)
                   aCTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTAt                                                                                                                            >  2:317378/1‑151 (MQ=255)
                    cTTCTCCGTCGTGCCGCCCCTCTCCTTTCATCTCTCCAACCCATTCCCCGACGTTATCTCCCCAGGCCGGAAGGTCGGGAGACTAATTTTCCTGCCCCAGTTTTTGAAGGGGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTAt                                                                                                                            <  2:746753/150‑1 (MQ=255)
                      tCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGtc                                                                                                                         >  2:916071/1‑151 (MQ=255)
                         aCGTCGTGACGCCACTCTTCTTTCATATCTTCAATACATTCACCGACGTTATCTTCCCAGGCAGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAAACCCACTGACCCCGCCGCACCTTTAATTTTATGtcctt                                                                                                                      >  2:937447/1‑151 (MQ=255)
                              gtgcccccACTCTTCTTTCATCTCTCCAATCCATCCCCCGACTTTTTCTCCCCAGGCGGGAAGGTCAGGAGACTGATTTGGCTGCCCCGGTTGTTGCAGTGGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTc                                                                                                                  <  2:688602/144‑1 (MQ=255)
                               tGACCCCCCTCTTCTTTCATCTCTTCAATCCATCCCCCGCCGTTATCTCCCCAGGCCGGAAGGTCGGGAGACTGAATTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTca                                                                                                                 <  1:521341/150‑1 (MQ=255)
                                gACCCCCCTCTTTTTTCATCTCTTAAATCCATCCCCCGTCTTTATCTCCCCAGGCCGGAAGGTCAGGAAACTAAATTTCCTGCCCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTcaac                                                                                                               <  1:189126/151‑1 (MQ=255)
                                  cGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTcaaca                                                                                                              >  1:651246/1‑150 (MQ=255)
                                          ttcttTCATCTCTTCAACCCATTCACCGACGTTATCTCCCCAGGCGGGAAGGCCGGGAGACTGATTTTGCTGCCCCAGCTTTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCttt                                                                                                      <  1:874327/150‑1 (MQ=255)
                                          ttcttTCATCTCTTAAACCCATCCCCCGACGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGCCCCAGCTGTTGAAGATGGCGTACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCt                                                                                                        <  1:500556/148‑1 (MQ=255)
                                           tcttTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCttttt                                                                                                    >  2:587380/1‑151 (MQ=255)
                                              ttCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTAt                                                                                                 >  1:578201/1‑151 (MQ=255)
                                              ttCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTAt                                                                                                 >  2:196260/1‑151 (MQ=255)
                                                cATCTCTTCAACCCATCCCCCGCCGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTAAATTTGCTGCCCCAGTTTTTGAAGATGGCGTACCCCCCCGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATc                                                                                                <  2:192008/150‑1 (MQ=255)
                                                cATCCCCTCAACCCATCCCCCTACCTTATCTCCCCAGGCCGGAAGGGCAGGAGACTGATTTTGCTGCCCCAGCTTTTGAAGATGGCGAACCCCCTCTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTa                                                                                                  <  2:737040/148‑1 (MQ=255)
                                                 aTCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCt                                                                                              >  1:151633/1‑151 (MQ=255)
                                                     ctTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTgcgc                                                                                          >  2:300954/1‑151 (MQ=255)
                                                         aacccATCCCCCGACTTTATCTCCCCAGGCCGGAAGGTCAGGAGACTAATTTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTgcg                                                                                           <  1:443518/143‑1 (MQ=255)
                                                         aacccATCCCCCGACGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGATTTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTgcgc                                                                                          <  1:109497/144‑1 (MQ=255)
                                                            ccATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTc                                                                                      >  1:733418/1‑148 (MQ=255)
                                                            ccATCCCCCGACTTTATCTCCCCAGGCGGGAAGGCAAGGAGCTTAATTTTCCTCCCCCGGTTTTGGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAg                                                                                    <  1:489474/150‑1 (MQ=255)
                                                             cATCCACCGACTTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGt                                                                                       <  1:935794/146‑1 (MQ=255)
                                                                tCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCg                                                                               >  2:587088/1‑151 (MQ=255)
                                                                tCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCg                                                                               >  1:73101/1‑151 (MQ=255)
                                                                    cGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTc                                                                           >  1:693389/1‑151 (MQ=255)
                                                                     gACGTTATCTCCCCGGGCCGGAGGGCCAGGAGGCGAATTTGCCGCCCCCAGTTTTGGCAGATGGCGAACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTc                                                                           <  2:856095/150‑1 (MQ=255)
                                                                         ttttctcCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGCCCCAGTTTTTGCAGATGGCTTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGc                                                                       <  1:414620/147‑1 (MQ=255)
                                                                                cccggccgggAGGGTCAGGAGACTGATTTTGCTCCCCCAGCTGTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCt                                                                  <  1:37834/140‑1 (MQ=255)
                                                                                  cAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCACGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACa                                                              >  1:511266/1‑150 (MQ=255)
                                                                                   aGGCCGGAGGGTCAGGAGACTTATTTTGCTGACCCATCTTTTGCAGATGGCTTACCCCCTCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACAt                                                             <  2:629761/150‑1 (MQ=255)
                                                                                   aGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACAt                                                             >  2:182579/1‑150 (MQ=255)
                                                                                   aGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACAt                                                             >  2:725647/1‑150 (MQ=255)
                                                                                    ggCCGGAAGGCCAGGAGACTGATTTTGCTGCCCCATTTGTGGAAGAGGGCTAAACCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGAc                                                               <  2:584893/147‑1 (MQ=255)
                                                                                        ggAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCg                                                       >  2:148184/1‑151 (MQ=255)
                                                                                          aaGGTCAGGAGACTAATTTGGCGACCCCAGCTGTTGCAGATGGCGTACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCgg                                                      <  1:509111/150‑1 (MQ=255)
                                                                                              tCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGcatc                                                  >  2:459857/1‑150 (MQ=255)
                                                                                              tCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGcatc                                                  >  2:522394/1‑150 (MQ=255)
                                                                                                         aaTTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAac                                      >  2:357045/1‑151 (MQ=255)
                                                                                                           tttGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAacac                                    >  2:410579/1‑151 (MQ=255)
                                                                                                           ttggCTGACCTACCTGTTGCAGATGACATACCCCCCGCCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAaca                                     <  1:71537/147‑1 (MQ=255)
                                                                                                                tGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCt                                >  1:749052/1‑150 (MQ=255)
                                                                                                                 ctcccccAGTTGTTGAAGGTGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAacac                                    <  1:579989/143‑1 (MQ=255)
                                                                                                                 ctcccccAGCTGTTGAAGATGGCGAACCCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCg                                  <  2:181603/145‑1 (MQ=255)
                                                                                                                   aCCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATCCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAAc                             >  2:862679/1‑151 (MQ=255)
                                                                                                                           tGTTGCGGTTGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTc                        <  1:744334/149‑1 (MQ=255)
                                                                                                                                 aGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATc                 >  1:52894/1‑150 (MQ=255)
                                                                                                                                  gATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATc                 >  2:528112/1‑149 (MQ=255)
                                                                                                                                  gATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATc                 >  2:603566/1‑149 (MQ=255)
                                                                                                                                     ggtttacccccccTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATc                 <  2:863177/139‑1 (MQ=255)
                                                                                                                                        taacccccccTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACGCCGCTAACCCGTCGGTGATCa                <  1:106313/140‑1 (MQ=255)
                                                                                                                                            ccccccTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTc       <  1:878146/149‑1 (MQ=255)
                                                                                                                                              ccACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTAc   <  1:315644/151‑1 (MQ=255)
                                                                                                                                                aCTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACa  >  2:541720/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACA  >  NC_000913/3350707‑3350998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: