Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 501,575 | 0 | A | C | 44.3% | 102.1 / 18.6 | 61 | V555G (GTG→GGG) | ybaL | inner membrane putative NAD(P)‑binding transporter |
Reads supporting (aligned to +/- strand): ref base A (30/4); new base C (0/27); total (30/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.25e-13 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATT > NC_000913/501428‑501723 | aaGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCt > 2:599921/1‑151 (MQ=255) gTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTc > 1:350533/1‑150 (MQ=255) gTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTCGCTCTCGATGCCTGATGCGACCCTTGCGCGTCTTCTCATGCCTCCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTc < 2:2876/150‑1 (MQ=255) aTCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCAccc < 1:109206/151‑1 (MQ=255) tcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACccgcc < 2:528543/150‑1 (MQ=255) gTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGt > 2:656861/1‑150 (MQ=255) cGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCg > 1:428454/1‑151 (MQ=255) ccAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTcc > 1:278469/1‑150 (MQ=255) cAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTc > 2:581305/1‑148 (MQ=255) ggggTTCACGCCTCATCTGTCATTTGGCTCTCGGTGCCTGATGCGCCGCGGGCGCTTCTTATCATCCCTACTTTTTTTTCATATTTTCCCTCCGGCAACCACCTTTTCCCCCGT‑CCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTcc < 2:811185/148‑1 (MQ=255) ggCTTTCACGCCGCATCTGGCTTTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATTTTTTTCATATTTTACACCCGGCACCCACCTTTTCCCCCGC‑CACCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTcc < 2:444829/151‑1 (MQ=37) ggCTTTCACGCCGCATCTGGCTTTTGGCTCCCGATGCCTGATGCGACGCGGGCGCGTCTTATCATGCCTAAATATTTTTCATATTTTACACCCGGCACCCCCCGTTTCCCCCGC‑CCCCCCCTCCCCCGCCGGTGGCGTTTCcagcag < 1:837803/147‑1 (MQ=255) cACGCCGCATCTGGCTTTTGGCTCTCGATGCCTGATGCGCCGCTGGCGCGTCTTATCATCCCTACATTTTTTTAATTTTTTCCACCCGGCACCCACCGTTTCCCCCGT‑CCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATg < 2:608277/151‑1 (MQ=255) cACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATg > 2:612332/1‑151 (MQ=255) cccctcATCTGGCTTTTGGCTCTCGATGCCTGATGCGACGCTGGCGCTTCTTACCATTCCTACATATTTTTCATATTTTACATCCGGCAACCGCCTTTTACCCCGT‑CCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGt < 1:122013/146‑1 (MQ=255) ccgcATTTGGCTTTTGGCTCTGGTGGCTTGATGCGACGCGGGCGCGTTTTATCATGCCTACATTTTTTTCATATTTTACATCCGGCACCCACCGTTTACCCCGC‑CACCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGt < 1:257861/149‑1 (MQ=255) ccgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTAc > 2:164953/1‑151 (MQ=255) ccgcATCTGGCATTTGGCTCTCAATGCCTGATGCGCCGCGGGCGCTTTTTATCATGCCTACATATTTTTCATATTTTACATCCGCCAACCACCTTTTCCCCCGT‑CACCACCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATgg < 1:503184/148‑1 (MQ=255) cccatttgggCTTTTGCCTCCCTATCCCGAATGCGCCCCTGGCGCGTCTTCCCTTCCCTACCTTTTTTACATTTTTTCCACCCGGCAACCCCCTTTCCCCCCGC‑CCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa < 2:390734/137‑1 (MQ=255) cTGACATTGGGCTCTCGATCCTTGTGGCGACGCGGGGGCGTTTTATCAGCCCTACATTTTTTTCATATTTCACATCCGGCAACCACCTTTTCCCCCTT‑CCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATg < 1:802447/141‑1 (MQ=255) aTTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTc > 1:804569/1‑150 (MQ=255) tttGGCCCTCGATGCCTGATGCGCCGCTGGCGCTTTTTATCATCCCTACATTTTTTTCATTTTTTACACCCGGCACCCACCTTTTCCCCCGC‑CCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTc < 2:759348/149‑1 (MQ=255) ggCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc > 2:567266/1‑150 (MQ=255) ggCTCTCGATGCCTGATGCGACGCGGGCGCGTCTTATCATCCCTACATTTTTTTCATATTTTACATCCGGCACCCACCGTTTCCCCCGT‑CCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc < 1:749074/150‑1 (MQ=255) aTGCCTGATGCGCCGCTGGCGCTTCTTATCATCCCTACATATTTTTCATATTTTACACCCGGCACCCACCGTTTCCCCCGC‑CCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCAt < 1:913639/150‑1 (MQ=255) tGCCTGATGCGCCGCTGGCGCTTCTTTTCATCCTTAAATTTTTTTCATTTTTACCACCCGGCACCCCCCGTTTCCCCCGT‑CCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGccc < 2:228201/147‑1 (MQ=255) tGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATc > 1:286693/1‑150 (MQ=255) gATGCGACGCTGGCGCTTTTTATCAGGCCTACATATTTTTCATTTTTTACACCCGCAAACCCCCTTTTCCCCCTT‑CCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATc < 2:852111/145‑1 (MQ=255) gCGACGCTGGCGCGTCTTATCATGCTTACATTTTTTTCATATTTTACACCCGGCACCCCCCGTTTCCCCCGT‑CCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAcc < 1:453943/148‑1 (MQ=255) aCGCTGGCGGGTTTTTTCATGCCTCCATATTTTACATATTTAACACCCGGCACCCCCCTTTTCCCCCGT‑CCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc < 2:544364/144‑1 (MQ=255) cGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCg > 2:929664/1‑151 (MQ=255) gcggccgctTCTTATCATCCCTACATTTTTTTCATATTTTCCACCCGGCACCCCCCTTTCCCCCCTC‑CCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTc < 2:632877/144‑1 (MQ=255) gCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGc > 2:256363/1‑151 (MQ=255) ggCGCGTTTTATCATGCCTACATTTTTTTCATTTTTTCCACCCGGCAACCCCCTTTTCCCCCGT‑CCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGca < 1:757528/149‑1 (MQ=255) ggCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacc > 2:353823/1‑151 (MQ=255) gcgcTTTTTATCATCCCTACTTTTTTTTCATTTTTTCCCCCCGGC‑ACCCCCGTTTTCCCCCTCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCt < 2:453492/140‑1 (MQ=255) cgTTTTATCATCCCTACATTTTTTTCTTTTTTTCCATCGGCCACCCCCCGTTTCCCCCCT‑CCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcaccac < 2:52063/149‑1 (MQ=255) ttttATCCTGCCTACATTTTTTTCATATTTAACATCCGGCACCCCCCGTTTCCCCCGT‑CCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt < 2:853312/148‑1 (MQ=255) cTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCg > 1:585388/1‑151 (MQ=255) tGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGatat > 2:305636/1‑151 (MQ=255) tGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGatat > 2:403891/1‑151 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGatat > 1:599959/1‑150 (MQ=255) tACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGc > 1:194988/1‑151 (MQ=255) cATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGcc > 1:402562/1‑150 (MQ=255) tttttttCATTTTTTCCACCCGGCACCCCCCGTTTCCCCCGC‑CCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGatat < 1:524919/141‑1 (MQ=255) tatTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACt > 2:542332/1‑151 (MQ=255) tttttCATATTTTACATCCGGCAACCCCCGTTTCCCCCGC‑CCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACt < 1:524539/149‑1 (MQ=255) tttttCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACtt > 1:815654/1‑150 (MQ=255) ttttCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTc > 2:168114/1‑150 (MQ=255) ttttACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCAt > 1:677074/1‑150 (MQ=255) aCATCCGGCACCCCCCGTTTCCCCCGT‑CCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATg < 1:350554/150‑1 (MQ=255) aCATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATgg > 2:557161/1‑151 (MQ=255) aTCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGgc > 1:167901/1‑151 (MQ=255) ccGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGgcgc > 1:289463/1‑151 (MQ=255) caaccccccttttCCCCCGC‑CCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCgg < 1:656954/140‑1 (MQ=255) cccccGTTTCCCCCGC‑CCCCCCCCCCCCCGCCGGTGGTGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGc < 2:632408/145‑1 (MQ=255) tttCCCCCGC‑CCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAtct < 2:705536/150‑1 (MQ=255) ccccGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAat > 1:596214/1‑150 (MQ=255) cccGT‑CCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAatat < 1:600011/151‑1 (MQ=255) ccGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATc > 2:583571/1‑151 (MQ=255) cGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATcc > 1:12663/1‑151 (MQ=255) ccaccTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGAtt > 2:302319/1‑151 (MQ=255) | AAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGT‑CACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATT > NC_000913/501428‑501723 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |