Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 646,747 | 0 | T | G | 47.1% | 77.3 / 13.8 | 52 | T255P (ACC→CCC) | citG | 2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase |
Reads supporting (aligned to +/- strand): ref base T (4/23); new base G (24/0); total (29/23) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.27e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG‑CAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGA‑GCAAGGTATCGAGCAATGCCAGTTCAGGATC > NC_000913/646598‑646891 | tAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGt < 2:672437/150‑1 (MQ=255) aaTATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGtt < 2:383205/150‑1 (MQ=255) aaTATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGtt < 2:726021/150‑1 (MQ=255) aaTATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTc < 1:64695/151‑1 (MQ=255) tCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATg < 1:652771/150‑1 (MQ=255) cAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGttttgctgc > 2:154433/1‑142 (MQ=255) cAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑GATGccc > 1:748657/1‑151 (MQ=255) aaTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGccccc < 1:618819/151‑1 (MQ=255) aaTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑G‑GGTTccccc > 2:60384/1‑151 (MQ=255) tttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑GATGccccc > 1:618979/1‑149 (MQ=255) ttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑G‑AATGCCCCCctt > 1:727397/1‑151 (MQ=255) tATCAAGTGCCTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCtt < 1:381196/149‑1 (MQ=255) aTCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCtttt < 1:17762/150‑1 (MQ=255) aTCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCtttt < 2:150331/150‑1 (MQ=255) aTCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCtttt < 1:416059/150‑1 (MQ=255) tCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑GCAAGGCCCCCcttt > 2:519751/1‑150 (MQ=255) tCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑G‑AATGGCCCCctttt > 2:359362/1‑150 (MQ=255) aaGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCa < 2:611916/151‑1 (MQ=255) ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑G‑AATGACCCCTTTTTTCaata > 1:619252/1‑148 (MQ=255) tAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑G‑TATTGCCCCTTTTTTtaataa > 1:136029/1‑148 (MQ=255) ataatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTg < 1:465423/151‑1 (MQ=255) ataatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑G‑AATGGCCCCCTTTTTTAATAAAGtt > 1:342404/1‑149 (MQ=255) taatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑T‑AATGCCCCCTTTTTTCAAAAATAttt > 1:345049/1‑149 (MQ=255) aatTAAAGCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTgc < 2:238397/150‑1 (MQ=255) atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAGCTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑G‑GATGCCCCCCTTTTTCCATAATGttttc > 2:441087/1‑147 (MQ=255) atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTgcgc < 1:468000/151‑1 (MQ=255) atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGG‑AAGCCCCCCTTTTTGCataatttt > 2:28794/1‑139 (MQ=255) atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑G‑GATGCCCCCCTTTTTCCATAATttttt > 1:295932/1‑147 (MQ=255) aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCt < 1:404605/150‑1 (MQ=255) aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑G‑AAAAACCCCCTTTTTTAATAAAGTTTTCGCCTc > 1:444673/1‑151 (MQ=255) tgtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGGT‑G‑GATGCCCCCCTTTTTTAATAATGTTTTCGcc > 1:647938/1‑144 (MQ=255) tAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑G‑AATGCCCCCCTTTTTTAATAATGTTTGCGCCTCCCGCTGTAg > 1:351321/1‑150 (MQ=255) aaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTT‑G‑AATGCCCCCCTTTTTTAATAAAGTTTTTGCCCCTCGCCGTAGCCCgc > 2:833427/1‑151 (MQ=255) cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑GAAATGCCCCCTTTTT‑CCATAATTTTTTCGCCCCGCCCTGTTGcc > 1:434561/1‑145 (MQ=255) ggTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTG‑G‑ATGGCCCCCTTTTTG‑AAAAATTTTTGTGCCTcgc > 2:172051/1‑134 (MQ=255) ggTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTT‑G‑GAAGCCCCCCTTTTTTAATAATTTTTTGGCCCCGCGCCGTAGCCa > 2:335441/1‑144 (MQ=255) aGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGATC‑G‑GATGCCCCCCTTTTTTAATAATCGTTTCGCCTCCGGCTGTAgttcgcagaagcccc > 1:945395/1‑137 (MQ=255) ggATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGccccc < 2:561581/151‑1 (MQ=255) cAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTc < 1:652839/150‑1 (MQ=255) cAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTT‑G‑GATGCCCCCCTTTTTCCAAAATGTTTTCGCCTCCCGCCGTAGCCAGCGCAGGcccccccc > 1:795187/1‑148 (MQ=255) cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTT‑C‑AAAGCCCCCCTTTTTCCATAATGTTTTTGCCTCGCGCCGTAGCCAGCCCAGGCCCCCCCCGCccccc > 2:275617/1‑148 (MQ=255) cgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG‑CAAc < 2:943907/151‑1 (MQ=255) tGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTT‑G‑AATGCCCCCTTTTTTCAATAATTTTTTCGCCCCTCGCTGTAGCCAGCGCAAGCCCCCCTCCCCACCCCGCGAGG‑CAACGtt > 1:23626/1‑150 (MQ=255) ggACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG‑CAACGtt < 2:311733/149‑1 (MQ=255) gACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG‑CAACGTTGGt < 1:19280/151‑1 (MQ=255) aCTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTG‑G‑AATGCCCCCCTTTTTCCATAATGTTTGCGCCCCCCGCCGTAGCCAGCGCAGGCCCCCCTCCCCCCCCCGCGCTG‑CAACGtt > 2:667693/1‑147 (MQ=255) aTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG‑CAACGTTGGTATCGCCg < 1:705447/151‑1 (MQ=255) ttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTC‑G‑CATGGCCCCCTTTTTCAATAATATTTTCGCCTCGCCCCGTAGCCCGCGCCGGCCCCCCCCCCCCCCCCCCCCTG‑GAACGTTGGGATTGCCGCTGAtt > 2:901531/1‑150 (MQ=255) cgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG‑CAACGTTGGTATCGCCGTTGATCg < 2:917929/150‑1 (MQ=255) aTACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG‑CAACGTTGGTATCGCCGTTGATCGCCa < 2:15247/151‑1 (MQ=255) aTACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG‑CAACGTTGGTATCGCCGTTGATCGCCa < 1:48310/151‑1 (MQ=255) cccTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG‑CAACGTTGGTATCGCCGTTGATCGCCATCAGTAg < 2:414105/151‑1 (MQ=255) cGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTT‑G‑AATGGCCCCCTTTTTCCATAATATTTTTGCCCCGCGCCGGAGCCCGCGCAGGCCCCCCCCGCCCCCCCCCGATG‑CAAAGGTGGTATCGCCGGTGATAGCCCTCACTAgg > 2:787968/1‑146 (MQ=255) gCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG‑CAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGA‑GCAAGGTAt < 1:715873/150‑1 (MQ=255) gagaTAATCGAGATCGGC‑GGGGGGTT‑G‑AAAGCCCCCCTTTTTCTAAAATATTTTCGCCCCCCGCTGTAGCCAGCGCCGGGCCCCCCCCCCCCCCCGCCGTGCCAACG‑TGGGATTGCCGGTGATTGCCCACAGTAGGAGGCAAggatc > 2:849976/1‑144 (MQ=255) c‑ggGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG‑CAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGA‑GCAAGGTATCGAGCAATGCCAGTTCAGGATc > 1:596689/1‑151 (MQ=255) | TAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTC‑G‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG‑CAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGA‑GCAAGGTATCGAGCAATGCCAGTTCAGGATC > NC_000913/646598‑646891 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |