Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,187,214 A→C D622A (GAC→GCC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,187,2140AC98.3% 218.8 / ‑3.8 58D622A (GAC→GCC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (30/27);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01

ACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGG  >  NC_000913/4187066‑4187361
                                                                                                                                                    |                                                                                                                                                   
aCTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGccc                                                                                                                                                   >  1:886427/1‑151 (MQ=255)
aCTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGccc                                                                                                                                                   >  1:751195/1‑151 (MQ=255)
 cTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGccc                                                                                                                                                   >  2:871044/1‑150 (MQ=255)
   gTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGa                                                                                                                                                <  1:871645/151‑1 (MQ=255)
       gCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCAt                                                                                                                                            <  1:53578/151‑1 (MQ=255)
           tGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTac                                                                                                                                        >  2:320402/1‑151 (MQ=255)
             ccATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTacac                                                                                                                                      <  1:90836/151‑1 (MQ=255)
                        atgatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCt                                                                                                                           <  1:944444/151‑1 (MQ=255)
                          gatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTAt                                                                                                                         <  2:374897/151‑1 (MQ=255)
                             tGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGc                                                                                                                       <  2:27284/150‑1 (MQ=255)
                                   gAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGt                                                                                                                <  1:51514/151‑1 (MQ=255)
                                      aGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCt                                                                                                             >  1:722671/1‑151 (MQ=255)
                                        gTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAACCCGACCGTTATTTTTGCGGCCCAGAACATGTACACCGGCTTCGCCTATGCAGCGCGTTCTgg                                                                                                           <  2:549695/151‑1 (MQ=255)
                                                  cTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGtt                                                                                                 >  1:367364/1‑151 (MQ=255)
                                                      aTCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGt                                                                                              >  2:264848/1‑150 (MQ=255)
                                                      aTCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGt                                                                                              >  1:909067/1‑150 (MQ=255)
                                                        cGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTAt                                                                                            <  2:390001/150‑1 (MQ=255)
                                                                aGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACAt                                                                                   >  2:560012/1‑151 (MQ=255)
                                                                 ggCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCGTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATg                                                                                  <  2:466723/151‑1 (MQ=255)
                                                                      tGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCAt                                                                             >  2:903175/1‑151 (MQ=255)
                                                                       gggTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCAt                                                                             <  2:770854/150‑1 (MQ=255)
                                                                         gTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATccc                                                                          <  1:284112/151‑1 (MQ=255)
                                                                            aaaaaGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCgg                                                                        >  2:239214/1‑150 (MQ=255)
                                                                            aaaaaGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCgg                                                                        >  1:77730/1‑150 (MQ=255)
                                                                            aaaaaGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGga                                                                       <  1:287969/151‑1 (MQ=255)
                                                                              aaaGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCgg                                                                        >  1:188977/1‑148 (MQ=255)
                                                                                        ccAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAacac                                                             <  2:751097/149‑1 (MQ=255)
                                                                                         cAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGa                                                           >  1:748678/1‑150 (MQ=255)
                                                                                            aaTGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGAGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAatc                                                       >  1:151547/1‑151 (MQ=255)
                                                                                              tGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAatcat                                                     >  2:187989/1‑151 (MQ=255)
                                                                                                cTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAatcatc                                                    >  2:342047/1‑150 (MQ=255)
                                                                                                cTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCAtct                                                   >  2:934418/1‑151 (MQ=255)
                                                                                                 tGAACACCTGCTACCGCATTCTCGGTCTGATACCGACCGTTATTTTTGCGGCCCAGGTCATTTACACCGGCTACGCCGATGCAGCGCGTTCTGGTGCATCTGTTGGTCGCGATGACATGGTCATCCCGGAGCAGAAGCTCGAAatcag                                                     <  2:834746/148‑2 (MQ=255)
                                                                                                  gAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTcc                                                 >  1:885826/1‑151 (MQ=255)
                                                                                                   aaCACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCg                                                >  2:455374/1‑151 (MQ=255)
                                                                                                   aaCACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCg                                                <  2:603455/151‑1 (MQ=255)
                                                                                                   aaCACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCGCCg                                                <  2:482042/151‑1 (MQ=255)
                                                                                                     cacCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAg                                              <  2:323097/151‑1 (MQ=255)
                                                                                                      acCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAgg                                             >  1:667727/1‑151 (MQ=255)
                                                                                                          gCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgc                                            >  2:412166/1‑148 (MQ=255)
                                                                                                           cTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcaga                                         >  2:765116/1‑150 (MQ=255)
                                                                                                             aCCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaag                                       <  1:887509/150‑1 (MQ=255)
                                                                                                             aCCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaa                                        <  1:3649/149‑1 (MQ=255)
                                                                                                              ccGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaagca                                     <  1:554241/151‑1 (MQ=255)
                                                                                                                 cATTCTCGGTCTGAAACCGCCCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGCGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaagca                                     >  1:170360/1‑148 (MQ=255)
                                                                                                                 cATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaagcaga                                   >  1:787626/1‑150 (MQ=255)
                                                                                                                     ctcGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTg                              >  1:330031/1‑151 (MQ=255)
                                                                                                                      tcGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGc                             >  1:766676/1‑151 (MQ=255)
                                                                                                                            tGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAAt                       >  1:613181/1‑151 (MQ=255)
                                                                                                                             gAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAAt                       <  1:22384/150‑1 (MQ=255)
                                                                                                                              aaaCCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTc                     >  1:774546/1‑151 (MQ=255)
                                                                                                                              aaaCCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTc                     >  2:449461/1‑151 (MQ=255)
                                                                                                                                   gaccgTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAg                <  2:560702/151‑1 (MQ=255)
                                                                                                                                         tATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGtt           <  1:328104/150‑1 (MQ=255)
                                                                                                                                          aTTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTcc         >  1:344709/1‑151 (MQ=255)
                                                                                                                                            ttttGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTcc         >  1:639512/1‑149 (MQ=255)
                                                                                                                                            ttttGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCa        <  1:632087/150‑1 (MQ=255)
                                                                                                                                            ttttGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCa        <  1:112633/150‑1 (MQ=255)
                                                                                                                                               tGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAgtc     <  1:117543/150‑1 (MQ=255)
                                                                                                                                                 cGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAgtctgg  <  2:82164/151‑1 (MQ=255)
                                                                                                                                                 cGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAgtctgg  >  2:852840/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGG  >  NC_000913/4187066‑4187361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: