Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,265,009	C→A	intergenic (+39/‑3)	hemA → / → prfA	glutamyl tRNA reductase/peptide chain release factor RF‑1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,265,009	0	C	A	100.0%	305.5 / NA	77	intergenic (+39/‑3)	hemA/prfA	glutamyl tRNA reductase/peptide chain release factor RF‑1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (33/44);  total (33/44)

AACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGTTT  >  NC_000913/1264861‑1265156                                                                                                                                                     |                                                                                                                                                    aaCTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACt                                                                                                                                                   <  1:455138/151‑1 (MQ=255)    tGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATg                                                                                                                                                >  1:846795/1‑151 (MQ=255)     gACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTAt                                                                                                                                                 <  1:805389/149‑1 (MQ=255)      aCTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGaa                                                                                                                                              <  1:884435/151‑1 (MQ=255)      aCTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTACGa                                                                                                                                               >  2:826710/1‑150 (MQ=255)       cTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTAt                                                                                                                                                 >  1:780541/1‑147 (MQ=255)         aaCCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGc                                                                                                                                            <  2:735404/150‑1 (MQ=255)          aCCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCt                                                                                                                                          >  1:741408/1‑151 (MQ=255)           ccGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCtt                                                                                                                                         <  2:207362/151‑1 (MQ=255)           ccGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCtt                                                                                                                                         <  2:22761/151‑1 (MQ=255)             gCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCt                                                                                                                                       <  2:275049/151‑1 (MQ=255)             gCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCt                                                                                                                                       <  2:871982/151‑1 (MQ=255)              cTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCt                                                                                                                                       <  2:179516/150‑1 (MQ=255)               ttGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTAt                                                                                                                                     >  1:457495/1‑151 (MQ=255)                 gATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATc                                                                                                                                    <  2:590715/150‑1 (MQ=255)                 gATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCg                                                                                                                                   <  2:136916/151‑1 (MQ=255)                    ccATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGtt                                                                                                                                 <  1:600899/150‑1 (MQ=255)                    ccATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTg                                                                                                                                <  1:476485/151‑1 (MQ=255)                      aTGCGCCAACAAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGcc                                                                                                                              <  2:291461/151‑1 (MQ=255)                          gcCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAAc                                                                                                                          >  1:286520/1‑151 (MQ=255)                              aCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGa                                                                                                                      <  2:129323/151‑1 (MQ=255)                                gAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGa                                                                                                                      >  2:218671/1‑149 (MQ=255)                                gAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGa                                                                                                                      >  1:472450/1‑149 (MQ=255)                                gAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAg                                                                                                                    <  1:588030/151‑1 (MQ=255)                                     cACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTg                                                                                                               <  2:421107/151‑1 (MQ=255)                                        ttCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCAt                                                                                                            >  2:724582/1‑151 (MQ=255)                                              aGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGc                                                                                                      <  1:619923/151‑1 (MQ=255)                                                  cgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATg                                                                                                  <  2:613426/151‑1 (MQ=255)                                                    cccGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATga                                                                                                 >  1:271216/1‑150 (MQ=255)                                                        tGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATgaaga                                                                                              >  2:665057/1‑149 (MQ=255)                                                         gACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGt                                                                                           <  2:175538/151‑1 (MQ=255)                                                          aCGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGtt                                                                                          <  1:213181/151‑1 (MQ=255)                                                            ggggATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTc                                                                                         >  2:642924/1‑150 (MQ=255)                                                                          ccTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTg                                                                          >  1:525708/1‑151 (MQ=255)                                                                          ccTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTg                                                                          <  1:453950/151‑1 (MQ=255)                                                                           cTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTg                                                                          >  2:361170/1‑150 (MQ=255)                                                                             gAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGAcg                                                                       >  1:909329/1‑151 (MQ=255)                                                                                    cTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAAc                                                                >  1:105466/1‑151 (MQ=255)                                                                                       cgcgACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTAt                                                             <  1:614917/151‑1 (MQ=255)                                                                                          gACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGc                                                          <  1:785292/151‑1 (MQ=255)                                                                                          gACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGc                                                          >  2:859568/1‑151 (MQ=255)                                                                                           aCAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGcc                                                         <  2:48992/151‑1 (MQ=255)                                                                                              gCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGa                                                       >  2:527937/1‑150 (MQ=255)                                                                                              gCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGAc                                                      >  1:502797/1‑151 (MQ=255)                                                                                               ccTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGAcc                                                     <  2:552857/151‑1 (MQ=255)                                                                                               ccTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGAcc                                                     >  2:257672/1‑151 (MQ=255)                                                                                                  cGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAgg                                                  >  2:941940/1‑151 (MQ=255)                                                                                                      cTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACg                                              >  1:89834/1‑151 (MQ=255)                                                                                                      cTGGAGTAGCAGTACATCATTTTCTTTGTTTACAGGGTGCAGTTACGAATATGAAGCCTTCTATCGATGACAAACTGGAAGCCCGGCATGAACGCCATGAAGAAGTGCAGGCGGTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAAc                                               <  2:273226/150‑1 (MQ=255)                                                                                                        ggAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGt                                             <  1:296930/150‑1 (MQ=255)                                                                                                        ggAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGt                                             <  1:389286/150‑1 (MQ=255)                                                                                                         gAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGttt                                           <  1:704150/151‑1 (MQ=255)                                                                                                          aGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGtttt                                          >  2:660927/1‑151 (MQ=255)                                                                                                            tAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTc                                         >  2:753444/1‑150 (MQ=255)                                                                                                               cAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCgcgc                                     >  1:112332/1‑151 (MQ=255)                                                                                                               cAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCgcgc                                     <  2:284774/151‑1 (MQ=255)                                                                                                               cAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCgcgc                                     <  1:823390/151‑1 (MQ=255)                                                                                                               cAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCgcgc                                     >  1:539839/1‑151 (MQ=255)                                                                                                               cAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCgcgc                                     >  1:476528/1‑151 (MQ=255)                                                                                                                 gTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCAt                                   >  1:758828/1‑151 (MQ=255)                                                                                                                  tACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCAtt                                  >  1:437875/1‑151 (MQ=255)                                                                                                                      tcatTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATc                               >  2:220807/1‑150 (MQ=255)                                                                                                                        atTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCAcg                            <  2:457413/151‑1 (MQ=255)                                                                                                                        atTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCAcg                            <  1:513935/151‑1 (MQ=255)                                                                                                                         ttttCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCAcgc                           >  1:586074/1‑151 (MQ=255)                                                                                                                           ttCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCAcgcg                          <  1:842647/150‑1 (MQ=255)                                                                                                                           ttCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCAcgcg                          <  1:236047/150‑1 (MQ=255)                                                                                                                            tCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGa                         <  2:197240/150‑1 (MQ=255)                                                                                                                                tttttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAatat                     <  2:619681/150‑1 (MQ=255)                                                                                                                                  tttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAGCTATCGCCGACCAGGAGCGTTTTCGCGCATTATCACGCGAat                       <  2:214799/146‑1 (MQ=255)                                                                                                                                   ttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATgcgc                 >  2:653864/1‑151 (MQ=255)                                                                                                                                   ttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATgcg                  <  1:361237/150‑1 (MQ=255)                                                                                                                                          gTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAg          <  1:719544/151‑1 (MQ=255)                                                                                                                                               tttACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATg     >  2:372580/1‑151 (MQ=255)                                                                                                                                                ttACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATg     <  2:57261/150‑1 (MQ=255)                                                                                                                                                 tACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGt    <  1:607868/150‑1 (MQ=255)                                                                                                                                                  aCGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGttt  <  2:639896/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATGTTT  >  NC_000913/1264861‑1265156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑