Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,861,866	0	G	T	41.4%	90.6 / 35.0	58	Q37K (CAA→AAA)	yeaC	DUF1315 family protein
Reads supporting (aligned to +/- strand):  ref base G (10/24);  new base T (24/0);  total (34/24)
Fisher's exact test for biased strand distribution p-value = 1.12e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCTGAATCGTTTT  >  NC_000913/1861718‑1862014                                                                                                                                                      |                                                                                                                                                       aTAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTTCAGGGAGTTTTC‑tttttttt                                                                                                                                                      >  1:782752/1‑151 (MQ=255)  tAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGTCACCACATCACCCGTTGCCGGCCGGTCTC‑tttttttt                                                                                                                                                      >  1:399222/1‑150 (MQ=255)   aGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACCGCATCACCAGTTGCAGGCAGTTCTC‑tttttttt                                                                                                                                                      >  2:798772/1‑149 (MQ=255)   aGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTTCAGGCAGTTCTC‑tttttttt                                                                                                                                                      >  2:298578/1‑149 (MQ=255)      cTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAACATCACCAGTTGTAGGCAGTTCTC‑tttttttt                                                                                                                                                      >  2:276330/1‑146 (MQ=255)       tttGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCCCAGCATCAACAGTTGCAGGCAGTTCtt‑tttttttt                                                                                                                                                      >  1:624135/1‑145 (MQ=255)       tttGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGTCCCAGCACCACCAACTGCAGGCAGTtttt‑tttttttt                                                                                                                                                      >  1:613770/1‑145 (MQ=255)        ttGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCg                                                                                                                                                 <  1:602222/149‑1 (MQ=255)         tGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAACATCACCCGTTGCAGGCAGTTCTC‑TTTTTTTTTCTCgg                                                                                                                                                >  2:21006/1‑149 (MQ=255)          gCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGGTTTCCACAGCATCACCAGTTGCAGGCCGTTTTC‑tttttttttc                                                                                                                                                    >  2:15523/1‑142 (MQ=255)          gCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCCGGCAGTTCTC‑TTTTTTTTTCTCgg                                                                                                                                                >  2:463223/1‑148 (MQ=255)          gCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTc                                                                                                                                              <  2:448027/150‑1 (MQ=255)             gagaTACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTcaac                                                                                                                                           <  2:185001/150‑1 (MQ=255)               gaTACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTcaacgca                                                                                                                                        <  2:890868/151‑1 (MQ=255)                       aaTCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCCCAGCATCACCAGTTGCAGGGAGTTCTC‑TTTTTTTTTCTCGGGCAACGCAAcccc                                                                                                                                   >  2:284270/1‑148 (MQ=255)                           ttcctTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCCCAGCAACACCCGTTGCAGGCAGGTCTC‑TTTTTTTTCCTCTGGcaacgcaaacgccc                                                                                                                                 >  1:392119/1‑140 (MQ=255)                            tcttTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCCCAGCATCACCCAGTGCAGGCAGTTCTC‑TTTTTTTTTCTCCGTCAACGCCAcccccccgcgcc                                                                                                                           >  2:1095168/1‑143 (MQ=255)                               tttAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGcc                                                                                                                          <  1:436028/148‑1 (MQ=255)                                 tAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGGTTTCCACAGCATCCCCAGTTTCCGGGGGGTCTC‑TTTTTTTTTCTCGGTCAACGCAACGCCCCCA‑GGCCAtt                                                                                                                       >  2:619377/1‑149 (MQ=255)                                  aaCTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCACAACAACACCCATTGCAGGCAGTTCTT‑TTTTTTTTTCCCGGTCAACGCAAAGCCATCC‑GGCCATTTcc                                                                                                                    >  2:161667/1‑151 (MQ=255)                                   aCTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTccc                                                                                                                   <  2:880560/151‑1 (MQ=255)                                     tgctgTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCACAACACCACCCCCTGCAGGCGGGTCTT‑TTTTTTTTTCTCCGGCAACCCAACGCCATCA‑CGCCCTTTtcccc                                                                                                                 >  1:26659/1‑150 (MQ=255)                                      gctgTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAg                                                                                                                <  2:836376/151‑1 (MQ=255)                                             gCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCCCAACATCACCCGTTGTAGGCGGGTCTT‑TTTTTTTTTCTCGGGCTACGCCACGCCCTCA‑AGCCCATTccccccgtcaac                                                                                                          >  1:48049/1‑142 (MQ=255)                                               tctTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCCCAACATTACCAGTTTCAGGGAGATCTC‑TTTTTTTTTCTCCGTCAACGCCACGCCCTCC‑AGCCCTTTTCCCCGTTCTAcgg                                                                                                        >  1:782489/1‑150 (MQ=255)                                                  tCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCACAGCATCACCAGTTGCAGGCAGGTTTC‑TTTTTTTTCCTCGGGCAAAGCAACGCCATCA‑GGCCATTTCCCCCGTTCAAcggcggg                                                                                                    >  2:298820/1‑150 (MQ=255)                                                   cATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCCGTTGCAGGCAGTTCTC‑TTTTTTTTTCTCGGTCAACGCAACGCCATCA‑CGCCATTTCCCCAGTTCAAcggcgg                                                                                                     >  2:633929/1‑149 (MQ=255)                                                      gACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCCCAGCATCACCAGTTGCCGGCAGGTTTC‑TTTTTTTTTCTCGGTCAACGCAACGCCCTCA‑GGCCCTTTCCCCCGTTCAAcggcgg                                                                                                     >  2:579253/1‑146 (MQ=255)                                                        ccATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCCTGCCCCAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTTTTCCTCGGGCAACGCAACGCCCTCA‑GGCCCTTTTCCCCGTTTAACGGGGGGCg                                                                                                  >  2:488636/1‑147 (MQ=255)                                                          aTCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCCCAACATCACCAAGTGCAGGGAGTTTTC‑TTTTTTTTTCTCGGGCAACGCAACGCCACCA‑GGCCATTTTCCCCATTTAACGGCGGGCG‑Aaaaa                                                                                            >  1:641550/1‑150 (MQ=255)                                                          aTCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAACATCCCCCGTTGCCGGAAGATCTCTTTTTTTTTCCCCGGTC‑ACGCAACCCCCTCA‑GGCCCTTTTCCCAATTCCAAGGCGGGCG‑aacaa                                                                                            >  2:374457/1‑146 (MQ=255)                                                           tCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCCCAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTTTTTCTCCGTCAACCCCACGCCCTCA‑GGCCCCTTTCCCAGTTCAAacgcgggtcgcaaa                                                                                             >  1:853934/1‑141 (MQ=255)                                                            cTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTACAGGCAGTTCGC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACg                                                                                          <  1:219551/150‑1 (MQ=255)                                                              gACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCt                                                                                        <  2:276806/150‑1 (MQ=255)                                                              gACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTg                                                                                       <  2:377187/151‑1 (MQ=255)                                                              gACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTg                                                                                       <  2:955008/151‑1 (MQ=255)                                                                   tttGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGGTTGCCACAGCATCACCAGTTTCAGGGAGGTCTT‑TTTTTTTTTCTCTGTTAACGCAACACCCTCAGGGCCATTCCCCCCGTTAACcgggggcggtcaa                                                                                              >  1:969571/1‑134 (MQ=255)                                                                       tgtCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCCCAGCATCACCAGTTGCAGGCCGGTTTC‑TTTTTTTTTCTCGGGCAACGCAACGCCATCA‑GGCCCTTTTCCCCGTTCAACGGCGGGCG‑GCaaaaaccggggtaaccc                                                                              >  1:521971/1‑137 (MQ=255)                                                                           aaTTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTTCCCCAACATCACCAGTTGCAGGCAGGTTTC‑TTTTTTTTCCTCCGTCAACCCAACCCCCTCA‑GGGCCTTTTCCCCGGTCAACCGCGGGCGAACAAAcacggggtaactcccg                                                                            >  1:596726/1‑135 (MQ=255)                                                                                 cATATGCTGTGCTTCGATATTATGGCGGGCTTGGCCCAGCATCACCAGTTGCAGGCAGTTTTT‑TTTTTTTTTCTCCGTCCACGCAACGCCCTCA‑GGCCCTTTCCCCCGTTCAAcggcgggccgcaaa                                                                                             >  1:47181/1‑119 (MQ=255)                                                                                 cATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATg                                                                    <  1:1085481/151‑1 (MQ=255)                                                                                  atatGCTGTGCTTCGATATTATGGCGGGCCTGCCACAGCATCACCACTTGCAGGCAGGTTTC‑TTTTTTTTTCTCGGTCAACGCAAACCCCTCA‑GGGCATTTCCCCCGTTTAACGGCGGGCG‑GCAAAAACCGGTATAccccccg                                                                           >  2:568494/1‑138 (MQ=255)                                                                                    atGCTGTGCTTCGATATTATGGCGGGCCTGCCACCACATCACCACTTTGAGGCAAGTCTC‑TTTTTTTTTCCCCGGCAAAGCAACCCCCTCA‑AGGCCTTTCCCCCATTTAACAGCGGGCG‑ACAAAAACTGGTATTccacccgcctca                                                                      >  2:1102300/1‑136 (MQ=255)                                                                                        tgtgCTTCGATATTATGGCGGGCTTGCCACAGCATCCCCAGTTGCAGGCAGTTCTC‑TTTTTTTTTCTCGGTCAACGCAACGCCATCA‑GGCCATTTTCCCACTTCAACGGCGGGCG‑GCAAAAACCGGGATACCTCACGCATCCTTCTGt                                                                >  2:57272/1‑148 (MQ=255)                                                                                             ttCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTTCTCGGGCAACGCAACGCCATCA‑GGCCATTTTCCCCGTTCAAAGGCGGGCG‑ACAAAAGCCGGGATAACCCAAGCATCATGCTGtt                                                               >  2:1080860/1‑144 (MQ=255)                                                                                              tCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAAt                                                         <  2:661167/149‑1 (MQ=255)                                                                                                     tatGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCaa                                                 <  2:1571/150‑1 (MQ=255)                                                                                                      atGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAg                                                <  2:845194/150‑1 (MQ=255)                                                                                                        ggCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGAt                                              <  2:137468/150‑1 (MQ=255)                                                                                                             gCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATaa                                        <  2:61094/151‑1 (MQ=255)                                                                                                                tGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAAttt                                     >  2:840917/1‑151 (MQ=255)                                                                                                                   cacaGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGGATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAAttttt                                   >  1:645976/1‑150 (MQ=255)                                                                                                                     caGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCg                                <  1:459480/151‑1 (MQ=255)                                                                                                                      aGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCg                                <  2:1044786/150‑1 (MQ=255)                                                                                                                              cAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTgg                       >  1:710876/1‑151 (MQ=255)                                                                                                                              cAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTgg                       >  1:584525/1‑151 (MQ=255)                                                                                                                                gTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGaa                     <  1:944426/151‑1 (MQ=255)                                                                                                                                 ttGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATTTCATCAAGATTCATAATTTTTCCGCTCCTGTGGa                      <  1:687656/149‑1 (MQ=255)                                                                                                                                   gcaggcagTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAAt                    >  2:189862/1‑149 (MQ=255)                                                                                                                                    caggcagTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATa                   >  2:607910/1‑149 (MQ=255)                                                                                                                                     aggcagTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGt                >  2:1098475/1‑151 (MQ=255)                                                                                                                                     aggcagTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGt                <  1:1095032/151‑1 (MQ=255)                                                                                                                                       gcagTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGtt               >  1:224903/1‑150 (MQ=255)                                                                                                                                         agTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCt            <  1:877400/151‑1 (MQ=255)                                                                                                                                          gTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCTg           <  1:574989/151‑1 (MQ=255)                                                                                                                                                    ttGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCTGAATCGtttt  >  1:827372/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                       ATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTC‑TTTTTGTTCCTCGGTCAACGCAACGCCATCA‑GGCCATTTCCCCAGTTCAACGGCGGTCG‑ACAAACGCTGGTATACCTCAGGCATCATGCTGTTGATAATGTCATCAAGATTCATAATTTTTCCGCTCCTGTGGAATAAGTTGCTGAATCGTTTT  >  NC_000913/1861718‑1862014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑