| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 75,079 | 0 | C | T | 41.8% | 69.1 / 39.1 | 55 | L134L (CTG→CTA) | thiB | thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base C (4/28); new base T (23/0); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.40e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTT > NC_000913/74934‑75228 | aGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGtt < 1:145010/150‑1 (MQ=255) ttGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTtgtttgtt > 2:561958/1‑150 (MQ=255) tgtgGGGCGTCACCGCCATAGTCTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGtt < 2:159878/149‑1 (MQ=255) ggCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCa < 2:295551/151‑1 (MQ=255) gCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTTGTtttttttttttat > 2:910416/1‑146 (MQ=255) cATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTAGTTTTTTTTTTTTATaaa > 1:401907/1‑150 (MQ=255) tCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTTTGTTTTTTTTTTTTATaaa > 1:964781/1‑148 (MQ=255) gCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTTGTttttttttttttttaa > 1:317745/1‑138 (MQ=255) ttttGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTTGTtttttttttttat > 1:160778/1‑128 (MQ=255) tttGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTAGTTTGTTTTTTTTATAAAAGAAgggggaggagg > 1:1077197/1‑141 (MQ=255) tCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTATTTTGTTTTTTTTCTTAAAGAAAGCGaaagaaccatca > 1:732513/1‑139 (MQ=255) ccATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCa < 2:486176/151‑1 (MQ=255) ccATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCa < 2:756836/151‑1 (MQ=255) tAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTTTTTTTTTTTTTTTATTAAAGAAGGCGCA‑GGA‑GCCCTAATTaaa > 1:258866/1‑150 (MQ=255) cAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑g < 2:984598/149‑1 (MQ=255) aaCAGCCCCAGCCCCGGTGTCCTGGTGCGCGGACCTTGATAACTCCCCCGCCAGTTTTGATCGCTCTCATCCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTa < 1:991239/151‑1 (MQ=255) aCAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAc < 1:5743/151‑1 (MQ=255) aCAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTTATTTTGTTTTTTTCTTTAAAGCAAGCGCA‑GTA‑GTCACAATCaaaac > 1:387107/1‑145 (MQ=255) gACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑g < 2:28103/149‑1 (MQ=255) aCCCAGCCCCGGTGTACTGGTGCGCGGATCCTAATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑g < 1:721638/148‑1 (MQ=255) cccAGCCGCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑Ga < 2:413440/148‑1 (MQ=255) ccccGGTGTACTGGTGCGCGGCTCCTGATAATTCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCa < 2:153843/151‑1 (MQ=255) cGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTTCTTTGTTTTTGTTATAAAACAAAGCGGA‑AGA‑GGCAAAATTAAACGGGTAC‑‑AAA‑GGGTCatt > 2:473885/1‑150 (MQ=255) ggTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCatta < 2:840005/150‑1 (MQ=255) tgtACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCattatt < 2:1117658/150‑1 (MQ=255) cTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTc < 1:607903/147‑1 (MQ=255) tGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTACTTTGTTGTTTTCCTAAAAAAAGTGGAA‑AAA‑AGCACAAAAAAAA‑GGGAC‑GAAA‑GTGTTTTTATTcc > 2:347363/1‑147 (MQ=255) gTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTTGTTTTTTTTTTTTTTAAAAGAAGGGGGA‑GTA‑GCCCTTAACCAAA‑GGGAC‑GAAA‑AGGGCATTTTTTCAtccgc > 2:600286/1‑151 (MQ=255) cgcgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTAATTTGTTTTTGTCTTAAATGAAAGAGAAGGAA‑GCCA‑AATAAAAC‑AGATC‑GAAA‑GTGGCATTATTTCAagcgcccg > 2:103228/1‑149 (MQ=255) gcgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGGTTTTTTTTTTTC‑TAAAAAAAAGGGAA‑GGA‑ACCCAAATTAAAA‑CGGAC‑GAAA‑AGGTCATTTTTTCAACCGCC‑cgg > 2:1020552/1‑151 (MQ=255) ggtcctggATAAACCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGaa < 1:231093/144‑1 (MQ=255) aTCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTTGTTTTTTTTTTTCATAAAACAAAGGGAA‑GGA‑GGCCTAATCAAAA‑GGGAC‑GAAA‑AGGTCATTATTTCCACCCCC‑gggg > 1:582065/1‑147 (MQ=255) catGATAAATCGCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTGTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGt < 1:159565/148‑1 (MQ=255) cTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTTGTTTTTTTTTTTTTTAAAAGAAAGCGAA‑GTA‑GGCATAATCAAAA‑GGGAC‑GAAA‑GAGTCATTATTTCAGCCCCC‑GGGGACGt > 1:951164/1‑149 (MQ=255) tAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAAc < 2:1110143/149‑1 (MQ=255) cccccGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATc < 1:1111594/148‑1 (MQ=255) ccGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCt < 1:672609/151‑1 (MQ=255) cGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTg < 2:872419/151‑1 (MQ=255) cGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTTGTTTGTTTTTTTGATAAAAGCAAGCGAA‑GGA‑GGCATAATTAAAC‑AGGAC‑GGAA‑GTGTCATTATTTCCGCCGCCGGGGACCGTTAAAggattcgc > 1:856764/1‑143 (MQ=255) cGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGCTTTTTGTTTTTTTTTTTTTCTAAACGAAGGCGAA‑GGA‑GCCATAATTAAAA‑GGGAC‑GAAA‑ATGGCCTTATTTTCACCGCC‑GGGGACGTTAAAGGCCTcccc > 1:175867/1‑147 (MQ=255) gCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTTTCTTTTTTCCTTTCTTTAACAAAGGCGCA‑GAA‑GACCAAAACAAAC‑AGTGC‑GAAAGGGGCCGTTTTTCCGCCCCCC‑GGGACGGTTAACggatcc > 2:134477/1‑142 (MQ=255) gTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTAGTTTTTTTTTTTCATAAAAGAAAGGGAA‑GGA‑GCCATAATCAAAA‑GGGAC‑GAAA‑GGGTCATTATTTCAGCCCCC‑GGGGACGTTAACGGCCTCCGCTGcc > 2:306704/1‑148 (MQ=255) cGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCttt < 2:976357/150‑1 (MQ=255) ctcAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTgg < 1:910292/149‑1 (MQ=255) ctcAACCAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTTGTTTTTTCTTTTTATAAAAAAAGGAGAA‑GGAGGCAAAAATAAAAG‑GGAAG‑GAAA‑CGGT‑ATTTTTTCAGCCGCC‑GGGGAAGGTAAAGGGATCCCCCGCCACACCGCcttt > 1:665580/1‑147 (MQ=255) cAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCaaa < 2:530605/151‑1 (MQ=255) cAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCaa < 1:1098632/150‑1 (MQ=255) ccAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAg < 2:1060786/151‑1 (MQ=255) ccAGTTCTTTCAGGCTTTGTGGC‑GGGGTTTTTATTTTTTTTTTTTCTTAAAACAAGGGGAA‑GGA‑GCCCTAATCAAAA‑AGTACGGAAA‑GGGT‑ATTTTTTCAGCCCCC‑CGGGACGTTAAAGGCATCCCCTGCCCCACCCCCTTTTGCcaa > 1:369531/1‑148 (MQ=255) tCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGGCCGGttt < 1:906052/150‑1 (MQ=255) ttGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTggc < 2:240308/150‑1 (MQ=255) ggC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGgcggcg > 1:992827/1‑150 (MQ=255) gggTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCt > 1:158537/1‑150 (MQ=255) gTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAAc > 2:415461/1‑151 (MQ=255) tttCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAgg > 2:601867/1‑151 (MQ=255) cAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGgtt > 1:649555/1‑150 (MQ=255) | AGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGC‑GGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAA‑GTA‑GCCATAATCAAAC‑GGTAC‑GAAA‑GTGTCATTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTT > NC_000913/74934‑75228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |