Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,112,578	0	T	G	41.7%	110.9 / 14.2	61	G88G (GGT→GGG)	yiiR	DUF805 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (5/30);  new base G (25/0);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 5.49e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTTTG‑TTGGCAC‑ACAAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAG  >  NC_000913/4112430‑4112725                                                                                                                                                      |                                                                                                                                                          aGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGTCGTGGATGCTGCTGGC‑GGGTaa                                                                                                                                                         <  1:940510/151‑1 (MQ=255)     tCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGtgaattg                                                                                                                                                     >  2:254909/1‑145 (MQ=255)        aCTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTgg                                                                                                                                                     <  1:1028585/148‑1 (MQ=255)         cTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAAATggggg                                                                                                                                                  >  2:1023987/1‑148 (MQ=255)          tCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAAATgggggg                                                                                                                                                 >  1:956647/1‑147 (MQ=255)             atatTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAAACGGGC‑GGtttt                                                                                                                                            >  2:1014675/1‑151 (MQ=255)               atTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTa                                                                                                                                           <  2:261863/150‑1 (MQ=255)                   aGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAACTGGGG‑GGTTTTTCCcgg                                                                                                                                      >  2:475368/1‑151 (MQ=255)                      ccGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGGAAATTGGGG‑GGTTTTCCCggggt                                                                                                                                    >  1:617610/1‑149 (MQ=255)                      ccGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGgtgt                                                                                                                                   <  1:60590/151‑1 (MQ=255)                        gcggcgTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGTAATGGGC‑GATTTTACCGGgtg                                                                                                                                    >  2:466529/1‑148 (MQ=255)                         cggcgTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCt                                                                                                                                 <  2:926116/150‑1 (MQ=255)                                 tGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGGAAGGGGG‑GGGTTTACCggggggtttgggatggg                                                                                                                        >  2:521481/1‑137 (MQ=255)                                       gtgtgCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAAATGGGG‑GGTTTTACCGGGGGGCT‑GG‑GAATGGGCGgggg                                                                                                                >  1:409977/1‑151 (MQ=255)                                        tgtgCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAAATGGGG‑GGTTTTTCCGGGGGGCT‑GG‑CAATGGGCgggggg                                                                                                               >  2:494840/1‑151 (MQ=255)                                          tgcttgctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAACTGGGG‑GGTTTTTACCGGTGTTTGGG‑CAAgggggggggggg                                                                                                              >  2:46718/1‑151 (MQ=255)                                           gcttgctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGT‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGc                                                                                                             <  1:1023450/150‑1 (MQ=255)                                           gcttgctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGc                                                                                                             <  2:547454/150‑1 (MQ=255)                                              tgctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGtt                                                                                                          <  1:656426/150‑1 (MQ=255)                                               gctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGtttt                                                                                                        <  2:1017685/151‑1 (MQ=255)                                               gctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAACCGGGG‑GGTTTTTCCCGGGGTCT‑GG‑CAATGGGGGGGGGGGGGgttt                                                                                                        >  1:465694/1‑151 (MQ=255)                                                   tGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGGTGCTGCTGGC‑GGGGAAATGGGG‑GATTTTTTCGGGGGTCT‑GG‑CAATGGGGGGGGGGGGGTTTTGt                                                                                                      >  2:780155/1‑149 (MQ=255)                                                    ggCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAACTGGGG‑GGTTTTTCCCGGGGGCT‑GG‑CCATGGGGGGGGGGGGGgttttttcc                                                                                                   >  2:47989/1‑146 (MQ=255)                                                       cAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAACTGGGC‑GATTTTTCCGGGGGTCT‑GG‑GCATGGGCGGGGGGGGGGTTTTTccc                                                                                                   >  2:388159/1‑148 (MQ=255)                                                         aCAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGt                                                                                              <  1:1055898/151‑1 (MQ=255)                                                              ggCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAAATGGGG‑GGTTTTTCCGGGGGGCTGGG‑AAAGGGGGGGGGGGGGGTTTTGTccgcaggtggtt                                                                                          >  1:19575/1‑141 (MQ=255)                                                               gCAGTAACTGTGAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTc                                                                                        <  1:754283/151‑1 (MQ=255)                                                                  gTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTgg                                                                                     <  2:383708/151‑1 (MQ=255)                                                                     aCTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGtga                                                                                  <  1:1028764/151‑1 (MQ=255)                                                                       tGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAACTGGGG‑GGTTTTTCCCGGGGGCT‑GG‑GCATGGGCGGGGGGGGGGTTTTTccccgccgtggtt                                                                                         >  1:364983/1‑132 (MQ=255)                                                                         ttAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAAATGGGG‑GTCTTTAACCGGGGTCC‑GG‑CGATGGGGGGTGGGGCGGTTTTTCCCCGCGGTGTTTCttggggt                                                                                 >  2:165923/1‑142 (MQ=255)                                                                            aGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCt                                                                           <  2:42882/151‑1 (MQ=255)                                                                              cgcTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTa                                                                         <  1:231822/151‑1 (MQ=255)                                                                               gcTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTAt                                                                        <  2:73634/151‑1 (MQ=255)                                                                                 ttGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAAATGGGG‑GGTTTTTTCGGGGGTCT‑GG‑GGATGGGGGGGGGGGGGGTTTTTTCCGGCGGTTGTTCTgggggtggtggttt                                                                         >  1:591506/1‑136 (MQ=255)                                                                                  tGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCg                                                                      <  2:834741/150‑1 (MQ=255)                                                                                    cATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATc                                                                   <  1:968774/151‑1 (MQ=255)                                                                                     aTGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCt                                                                  <  2:939527/151‑1 (MQ=255)                                                                                     aTGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAAATGGGG‑GGTTTTTTCCGGTGTTT‑TG‑CGATGGGGGGGGGGGGGGTTTGTTCCCACGTTGGTTCTGGGGGGGGTGTTTTTCGATCt                                                                  >  1:936530/1‑151 (MQ=255)                                                                                        atctctggCGTTCCGGCGCATGGGCATTTCTGATGCTTGTGGCGTGGATGCTGCTGGC‑GGGGCCATGGGG‑GGTTTTTCCGGGGGTCT‑GGCCAATGGG‑GGGGGGGCGGTTTTTCCCGGCGTTGGTTTTGGGGGTGATGCTTATCGGTCtt                                                                 >  2:837682/7‑149 (MQ=255)                                                                                        aTCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAAATGGGG‑GGTTTTTACGGGGGGGT‑GG‑GAAAGGGGGGGGGGGGGGTTTTTCCCGGAGGTTATTTTGGGGGTGGTGGTTATTGAtttttg                                                               >  2:837045/1‑149 (MQ=255)                                                                                          cgcgGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAACTGGGCGGTTTTTACCGGG‑GGCT‑GG‑GAAAGGGGGGGGGGGGGGTTTGTTCCCACGGTTATTCTGGGGGTGATGCTTTTCGATCTTgg                                                               >  2:398957/1‑149 (MQ=255)                                                                                            cgGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCg                                                          <  2:705119/151‑1 (MQ=255)                                                                                                   tCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTttg‑tt                                                   <  1:621638/150‑1 (MQ=255)                                                                                                         gcATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTTTG‑TTGGcac‑ac                                           <  2:533894/151‑1 (MQ=255)                                                                                                          cATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTTTG‑TTGGcac‑aca                                          <  1:563401/151‑1 (MQ=255)                                                                                                              ggCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTTTG‑TTGGCAC‑ACAAGGc                                      <  2:382277/151‑1 (MQ=255)                                                                                                                  tttCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAAATGGGC‑GGTTTTTCCCGGGGTCT‑GG‑CAATGGGGGGGGGGGGGGTTTTTCCCCCCGGTGATTCTTGGGGTGGTGCTTTTTGATCTTTGG‑GCGTTTG‑TTTGCAC‑ACAAAGCgga                                   >  2:1109132/1‑148 (MQ=255)                                                                                                                     cTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGTAATGGGG‑GGTTTTTTCGGGTGTCT‑GG‑CAAAGGGGGGGGGGGGGGTTTTTGCCGCCGGTTATTTTGGGGGTGATGGCTTTTGTTCTTTGT‑GGGTTTT‑TTGGGGCAACAAGGGGaaa                                  >  2:525853/1‑149 (MQ=255)                                                                                                                      tgatgaTTGTGGCGTGGATGCTGCTGGC‑GGGGAAATGGGG‑GGTTTTTCCCGGGGTGT‑GG‑CAATGGGGGGGGGGGGGTTTTTTTCCGGCGTTTGTTCTGGGGGTGGTGGTTATCCGTCTTTGTGGGGTTTT‑TGGGCCA‑ACAAGGGGaaa                                  >  1:441311/1‑148 (MQ=255)                                                                                                                        atgatTGTGGCGTGGATGCTGCTGGC‑GGGGAAATGGGG‑GGTTTTACCGGGGGTCT‑GG‑GAATGGGCGGGGGGGGGGTTTGTGCCGACGTTGGTTCTGGGGGTGATTGTTATCGAACTTTGT‑GGGGTTGTTTGGACC‑AAAAGGGGAaaaaaa                              >  1:411266/1‑150 (MQ=255)                                                                                                                           atTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTTTG‑TTGGCAC‑ACAAGGCGAAAATAAATATg                         <  1:786413/151‑1 (MQ=255)                                                                                                                              gtgGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTTTG‑TTGGCAC‑ACAAGGCGAAAATAAATATGGTa                      <  1:858152/151‑1 (MQ=255)                                                                                                                                ggCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTTTG‑TTGGCAC‑ACAAGGCGAAAATAAATATGGTaaa                    <  1:891689/151‑1 (MQ=255)                                                                                                                                  cGTGGATGCTGCTGGC‑GGGTAACTTGGC‑CGATTTATCGGGTGTCT‑GG‑GAAAGGGGGGTGGGGGGTTTTGTTGCGGCGGTGTTTTTGGTGGTGGTGCTTATTGATGTTGGT‑GCGTTTG‑TTGGCAC‑ACAAAGCGTAAATAAATATgg                        >  1:281851/1‑145 (MQ=255)                                                                                                                                   gTGGATGCTGCTGGC‑GGGTGACCAGGC‑GAATTTTCCGGGGGTCC‑GG‑CCATGGGCGGTGGGGCGGTTTGTGCCGACGCTGATTCTGGGGATGATGCTTATCGGTCTTGGT‑GCGTTTG‑TTGGCAC‑ACGAGGAGGAAATAAATATGGTAAAGa                  >  1:343940/1‑150 (MQ=255)                                                                                                                                   gTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGGGTCT‑GG‑CAATGGGCGGGGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTTTG‑TTGGCAC‑ACAAGGCGAAAATAAATATGGTaa                     >  1:695922/1‑147 (MQ=255)                                                                                                                                     ggATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTTCCGGGCGGCT‑GG‑CAAGGGGGGGCGGGGGGGTTTGTTCCGACGTTGGTTCTGGGGGTGATGGTTATCGATTTTGGT‑GGGCTTT‑TTGGGAC‑ACAAGGCGAAAATAAATTTGGGAAAGGTa                >  1:659670/1‑150 (MQ=255)                                                                                                                                         gctgctGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTTTG‑TTGGCAC‑ACAAGGCGAAAATAAATATGGTAAAGATACTCa            <  2:1016850/150‑1 (MQ=255)                                                                                                                                               ggC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTTTG‑TTGGCAC‑ACAAGGCGAAAATAAATATGGTAAAGATACTCAGGAtg       <  1:377777/149‑1 (MQ=255)                                                                                                                                                 c‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTTTG‑TTGGCAC‑ACAAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGa    >  1:700404/1‑150 (MQ=255)                                                                                                                                                   gggTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTTTG‑TTGGCAC‑ACAAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAg  <  2:838617/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                          AGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACTGGGC‑GATTTTACCGGGTGTCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGT‑GCGTTTG‑TTGGCAC‑ACAAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAAG  >  NC_000913/4112430‑4112725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑