Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,355,457 | 0 | A | C | 41.9% | 110.3 / 14.5 | 62 | T245P (ACC→CCC) | gltB | glutamate synthase, large subunit |
Reads supporting (aligned to +/- strand): ref base A (28/8); new base C (0/26); total (28/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.42e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCG > NC_000913/3355314‑3355604 | gcTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGt < 2:717461/149‑1 (MQ=255) gcTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTaa < 2:996099/151‑1 (MQ=255) gcTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAACGCTTCTCCGCTATCACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGt < 1:843727/149‑1 (MQ=255) aTCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgc > 1:743134/1‑151 (MQ=255) gACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcgtgcgc > 1:125602/1‑151 (MQ=255) aCCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcgtgcgcg > 2:1058931/1‑151 (MQ=255) ccTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcgtgcgcgt > 2:760137/1‑151 (MQ=255) gTCTGGAATCGGCCTTTTCCCTGTTCCCCCGGCGCTTCCCCACAAACCCCGTACCGCGCTGGCCGCTGGCCCACCCGTTCCGCTTTCGGGCGCATAACGGTGAAATCACCCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACtt < 2:493466/150‑1 (MQ=255) tCTGGAATCGGCCTTTTGCCTGTTCCCCCAGCGCTTCTCCACTACCCCCGTCCCGCGCTGGCCGCGGGCGCACCCGTCCCGCTATCTGGCGCATAACGGTGAAATCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTta < 2:125146/150‑1 (MQ=255) cTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTtat > 2:130313/1‑150 (MQ=255) tGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTtata > 2:536140/1‑150 (MQ=255) ggAATCGGCCTTTTGCCTTTTCCCCCAGCGCTTCTCCACAAACCCCGTCCCGCGCTGGCCGCTGGCCCACCCTTTCCGCTATCTGGCGCATAACGGTAAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATaaa < 2:190515/151‑1 (MQ=255) ggAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATaa > 2:918657/1‑150 (MQ=255) aTCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTc > 2:604914/1‑151 (MQ=255) aTCGGCCATTTCCCTGTTCCACCAGCGTTTCTCCACTAACCCCGTCCCGCGCTGGCCGCTGGCGAACCCGTTCCGCTTTCGGGCGAATAGGGGGAAAAACAACACCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAAtt < 2:283274/150‑1 (MQ=255) cGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTcc > 2:985396/1‑150 (MQ=255) ggCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTcc > 1:411871/1‑149 (MQ=255) cTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCtt > 2:463862/1‑150 (MQ=255) gTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATc > 1:607389/1‑151 (MQ=255) cccccaGCGCTTCCCCACAAACCCCGTCCCGCGCTGGCCGCTGGCGCACCCGTTCCGCTATCGGGCGCATAACGGTAAAATCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCt < 2:146339/148‑1 (MQ=255) caccaGCGCTTCTCCACTAACACCGCCCCGCGCGGGCCGCTGGCGCACCCGTCCCGTTATCGGGCGCATAAGGGTAAAATCAACCCCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTg < 2:601813/151‑1 (MQ=255) accaGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTg > 2:948103/1‑150 (MQ=255) caGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGAcc > 1:346834/1‑151 (MQ=255) aGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCt > 2:346028/1‑151 (MQ=255) ttCCCCACTAACCCCGCCCCGCGCGGGCCGCTGGCGCACCCTTCCCGCTATCTGGCGCATAACGGGGAAATCAACACCACCCCGGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCt < 2:472036/146‑1 (MQ=255) ctcCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCacgac > 1:674183/1‑151 (MQ=255) ccccACTAACCCCGTCCCGCGCGGGCCGCTGGCGCAACCGTCCCGCTATCGGGCGAATAACGGTAAAATCAACACCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCacga < 2:991111/148‑1 (MQ=255) ctacccccGTCCCGCGCTGGCCGCTGGCGCACCCGTCCCGCTATCTGGCGCATAAGGGTAAAATCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCacgacg < 2:651209/141‑1 (MQ=255) tAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGAcgccgc > 1:829089/1‑150 (MQ=255) ccccGTCCCGCCCGGGCCGCGGGCGCACCCGCACGCATATCTGCCAGATGCCGGTGAACACAACCCCATCCCCGGAAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCAc < 2:396593/147‑1 (MQ=255) cccccgcggcccgctggcCCACCCTTCCCGTTTTCTGGCCCATAACGGTAAAACCACCCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTc < 2:393217/141‑1 (MQ=255) ccGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTAAAATAAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCa < 1:411810/151‑1 (MQ=255) cgcgCTGCCCGTTGGCCCTCCCGTCCCGCTACCTGGCGCAAAGCGGTAAAACCAACACCATCACGGTAAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCaa < 1:273660/151‑1 (MQ=255) ggccgcggccgcACCCTTCCCGCTTTCTGGCGAATAACGGTAAAACCAACCCCATCACCGTAAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAAc < 1:225286/151‑1 (MQ=255) ggccgcggccgcACCCGTCCCGATATCGGGCGCATAACGGTGAAATCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACg < 1:82216/147‑1 (MQ=255) ccgctggcGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCg > 2:161166/1‑151 (MQ=255) ccgcgggcGCACCCGTTCCGTTATCTGGCGAATACGGGAAAAACCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAAc < 2:641303/149‑1 (MQ=255) gctggcGCACCCGTCCCGCTATCTGGCGAATAAGGGTAAAATAAACCCCTCCCCCGTAAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCgg < 2:130060/150‑1 (MQ=255) cccacccttcccGCTATCGGGCGCATAACGGTAAAATCACCCCCTTCCCCGTTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGct < 1:286270/137‑1 (MQ=255) cacccGTCCCGCTATCGGGCGCATAGGGGAAAAATCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGa < 1:632173/146‑1 (MQ=255) aaCCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTc > 1:673895/1‑151 (MQ=255) aCCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTcc > 1:122075/1‑151 (MQ=255) aCCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTcc > 1:693374/1‑151 (MQ=255) ccGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTcc > 1:286605/1‑150 (MQ=255) ccGTCCCGCTATCGGGCGCAAAGCGTTAAAATCACCCCCACCCCCGGAAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCa < 1:1058797/151‑1 (MQ=255) gTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGt > 1:496598/1‑151 (MQ=255) cGCTATCTGGCGCATAACGGTAAAATCAACACCATCCCCGTTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGt < 2:309450/147‑1 (MQ=255) cGCTATCTGGCGCATAACGGTAAAACCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGa < 1:94132/151‑1 (MQ=255) tATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATgg > 2:623856/1‑151 (MQ=255) aTCTGCCGAATACCGGAAAAACCAACCCCCTCCCGGTTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGtt < 2:176261/144‑1 (MQ=255) tCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGAt > 2:649865/1‑151 (MQ=255) cTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGAt > 2:1100971/1‑150 (MQ=255) cgcATAACGGTAAAATCAACACCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACa < 2:1101650/150‑1 (MQ=255) acgggtaaaacaaaCACCACCCCGGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACa < 1:288360/133‑1 (MQ=255) taaaaCCAACCCCACCCCGGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAAct < 1:648858/148‑1 (MQ=255) aaaaTCAACCCCACCCCGGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAAc < 1:791848/147‑1 (MQ=255) aaaaTCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAActg < 2:743239/149‑1 (MQ=255) aaaTCAACCCCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAActgct < 1:827903/151‑1 (MQ=255) aaCCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAActgctgc < 1:1114673/148‑1 (MQ=255) cacCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGc > 1:1020936/1‑150 (MQ=255) ccATCCCCGTTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAgg < 2:690681/151‑1 (MQ=255) aTCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCg > 2:100467/1‑151 (MQ=255) | GCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCG > NC_000913/3355314‑3355604 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |