| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 213,341 | 0 | A | G | 42.6% | 72.1 / 15.6 | 54 | G337G (GGA→GGG) | tilS | tRNA(Ile)‑lysidine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (10/21); new base G (23/0); total (33/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.33e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGC > NC_000913/213206‑213489 | tttACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGGAGGG‑GACAG‑CTTaa > 2:615666/1‑150 (MQ=255) aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGc < 2:943503/150‑1 (MQ=255) cGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGAGGCTTCAACCGCTGGAATTACCGGC‑GGGGCCGGGAAATG‑GACAA‑GTTAAt > 2:649631/1‑147 (MQ=255) gTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGGTGGGGAGGG‑TACAG‑CTTAATGCggg > 1:506617/1‑151 (MQ=255) tttGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGGTCGGAAGGG‑GACacacttattccggg > 1:468955/1‑138 (MQ=255) cgcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGGTGGGGAGGG‑GACAG‑CTTAAAGCGGGAgggg > 2:793859/1‑147 (MQ=255) gcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGGTGGGGAGTG‑TACAG‑CTTAATgggggggggg > 2:520424/1‑146 (MQ=255) tGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCg < 1:654386/150‑1 (MQ=255) aaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGGAGGG‑GACAG‑CTTAATGCGGGAGGGGAT‑ATTCGccc > 1:559139/1‑150 (MQ=255) gACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGGAGGG‑TACAG‑CTTAATGCGGGGGGCGAT‑ATTCGcccccccc > 2:210704/1‑148 (MQ=255) gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGGAGGG‑TACAG‑CTTAATGCGGGAGGCGGT‑ATTCGCCCCCCGCGTg > 1:383619/1‑150 (MQ=255) aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCa < 1:498748/149‑1 (MQ=255) aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCa < 1:592341/149‑1 (MQ=255) gTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGaa < 2:1084464/151‑1 (MQ=255) cGCAACTGTGGAGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGCGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGAGGTGGGACGGG‑TCACG‑CTTAATAAGCACGGCGCT‑ATTTGCCCACCGCCGGCAgcc > 1:413975/1‑144 (MQ=255) aaCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGGTGGGGAGGG‑GACAA‑GCTAAAACGGGAGGCGGT‑ATTTGCCCTCCCCGATCAGACGAAGAggg > 2:102693/1‑150 (MQ=255) aaCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGt < 2:130647/151‑1 (MQ=255) aaCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGCGTCGGGAAGGG‑TATAGACTAAATG‑GGGGGGCGAT‑TTTCTCCCTCCGCTTGGGGACggaa > 1:389126/1‑144 (MQ=255) aCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGGTGGGGAGGG‑TAAAG‑CTTAAAGCGGGGGGGGGT‑AATTGGCCCCCGCGGGCAg > 2:517874/1‑140 (MQ=255) aCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTc < 1:192406/151‑1 (MQ=255) gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgc < 2:1026943/151‑1 (MQ=255) tAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGGTGGGGAGGG‑TACAG‑CTTTATTCGGGAGGCGAT‑ATTTGCCCTCCCCGTGCAGACGAAGCGGGCAGCGTGCGgtt > 2:311280/1‑150 (MQ=255) aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGGAGGG‑GACAG‑CTTAATGCGGGGGGCGGT‑ATTTGCCCCCCGCGTGCAGACGAAACGGGCAGCGTGCGgtt > 2:634532/1‑149 (MQ=255) aaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGGTGGGGAGTG‑GACAG‑CTTAAATCGGGGGGGGAT‑ATTCGCCCCCCGCGGGCAGAAGAAGCGGGCAGCGCGCGgtt > 2:1109753/1‑148 (MQ=255) aaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGGAGGG‑TACAG‑CTTAATGCGGGAGGGGAT‑ATTTTCCCCCCGCCTGCCGAAGAAACGGGCAGCGTGCGttt > 2:107660/1‑148 (MQ=255) ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTTGGGAGGGT‑TACAC‑CTT‑ATCCGGGGGGCGATAATTTCCCCTCC‑CGTGCCGGAGAAGCGGGCTGCGGGCGGTTCaaa > 1:636462/1‑149 (MQ=255) ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAgc < 2:1099810/151‑1 (MQ=255) gTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGGAGGG‑TACAA‑CTTAAAGCGGGGGGCGAT‑AATTGCCCTCCCCGTGCAGACGAAGCGGGCAGCGTGCGGTTCcaa > 1:424075/1‑147 (MQ=255) aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATtgct < 2:133586/151‑1 (MQ=255) aaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATtgctg < 1:514894/151‑1 (MQ=255) gAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGGTGGGGAGGG‑TACAG‑CCTAATTCGGGGGGGGGT‑ATTTGCCCCCCCCGGGCCGGCGGAGGGGGCAGGGTGGGGTTTAAAACGCCCGGGAtgctgggt > 1:358717/1‑147 (MQ=255) aaaCATTGTGCCGTGTCAGACGTGGCTTCATCCGCTGGAATTACCGGG‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATAtt < 1:882022/151‑1 (MQ=255) aaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGGTGGGGAGGG‑TACAG‑CTTAATTCGGGAGGGGAT‑ATTTGCCCCCCCCGTGCAGACGAAGCGGGCAGCGGGCGTTTTAAAAGGCCAGGATTTCTGCATAtt > 2:376877/1‑150 (MQ=255) tgtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGGTGGGGAGTG‑GACAG‑CTTAAATCGGGGGGGGAT‑ATTTGCCCCCCCCGTGCCGAAGAAGGGGGTAGGGCGGGGTTTAAAACGCCAGGAATGATGCATATTTTCggg > 2:633839/1‑151 (MQ=255) gtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCggg < 1:179788/150‑1 (MQ=255) gtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGc < 1:158157/151‑1 (MQ=255) tgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGGTGGGAAGGG‑GACAG‑CTTAATGCGGGAGGCGAA‑ATTTGCCTTCCGCGGGCAGACGAAGCGGGCAGCGGGCGGTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCg > 2:962817/1‑151 (MQ=255) gCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGGAGGG‑TACAG‑CTTAATGCGGGAGGGGGT‑ATTTGCCCTCCGCGTGCAGACGAAGCGGGCAGCGTGCGTTTTAAAGCGCCCGGATTGGTGCATATTGTTGGGGGTAAcgg > 2:375998/1‑150 (MQ=255) gCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggc < 1:662524/151‑1 (MQ=255) gCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggc < 2:528624/151‑1 (MQ=255) aGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGGAGGG‑TACAG‑CTTAATGCGGGGGGGGGT‑ATTCGCCCCCCCCGTGCAGACGAAGAGGGCAGCGTGCGTTTCAAAGCGCCACGGTTTCTGCCTATTGTCGGGCGTAAacgcgg > 1:846211/1‑151 (MQ=255) aCGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGAc < 1:615574/151‑1 (MQ=255) tGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGGAGTG‑TACCG‑CTTAATGCGGGAGGGGGT‑ATTCGCCCTCCGCGTGGCGACGAAGCGGTCAGCGTGGGTTTCAAAGCGCCCGGAGTTCTGCATATTTTCGGGCGTAAcggggggagt > 2:899771/1‑146 (MQ=255) ggCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTa < 2:65173/150‑1 (MQ=255) tCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGc < 1:772568/149‑1 (MQ=255) cAACCGCTGGAATTACCGGC‑GGGGCTGGGGAGGG‑TACAA‑CTTTAAGCGGGGGGCGGT‑ATTTGCCCTCCGCGTGCAGACGAAGCGGGCAGCGGGCGTTTCAAAACGCCCGGATTGCTGCATATTTTTGGGGGGGAcggcgg > 1:29421/1‑140 (MQ=255) aaCCGCTGGAATTACCGGC‑GGGGGTGGGGAGGG‑GACAG‑CTTAATGCGGGGGGCGAT‑ATTCGCCCCCCCCGTGCAGACGAAACGGGCAGCGGGGGGTTTAAAACGCCCGGGTTGCTGCATATTGTCGGGGGGAAAGGCGGGCGGaaagca > 2:483692/1‑145 (MQ=255) aaCCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTaaa < 1:643985/151‑1 (MQ=255) aCCGCTGGAATTACCGGC‑GGGGCTGGGGAGGGTTCCGG‑CTTAA‑CCGGGGGGCGAA‑TCTTGCCCCCCGCCGGCAGAAGAAACGGGGAGCGGGCGCTTCAAACCGGCACGAGTGGTGCAGAATGATGGGCGTGTCGGCGGGCGTTAg > 1:911700/1‑145 (MQ=255) cTGGAATTACCGGC‑GGGGCTGGGAAAGG‑TACAG‑CTTAATGCGGGGGGCGAG‑ATTTGCCCTCCCCGGGCAGACGAAACGGGGCGCCGGCGTTTCAAAAAGCCAGGGTTGCTGGATATTGTCGGGCGTAAAGGGGGACGGAAACTCaaaaaa > 1:381930/1‑150 (MQ=255) cggc‑ggGGCTGGGAGGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAaga < 1:669889/151‑1 (MQ=255) c‑ggGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCt > 2:802178/1‑151 (MQ=255) tGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCg > 2:480535/1‑150 (MQ=255) ggAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgc > 2:189180/1‑151 (MQ=255) | TTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGC‑GGGGCTGGGAAGTG‑TACAG‑CTTAATGCGGGAGGCGAT‑ATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGC > NC_000913/213206‑213489 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |