Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,536,1970AT46.9% 34.7 / 46.9 49intergenic (+166/+417)yjhR/nanSpseudogene, helicase family; putative frameshift suppressor/9‑O‑acetyl N‑acetylneuraminic acid esterase
Reads supporting (aligned to +/- strand):  ref base A (2/24);  new base T (23/0);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 5.55e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGG  >  NC_000913/4536062‑4536343
                                                                                                                                       |                                                                                                                                                   
gtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGGTTTTTTTAATGaaaaaaaa                                                                                                                                       >  1:867856/1‑150 (MQ=255)
gtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAAt                                                                                                                                      <  2:448903/151‑1 (MQ=255)
 taAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGTTGGTTTTTTGGATGAAAGaaaa                                                                                                                                       >  1:505241/1‑149 (MQ=255)
  aaaGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATg                                                                                                                                     <  2:1108514/150‑1 (MQ=255)
        cATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGTATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagc‑a                                                                                                                              <  1:943820/150‑1 (MQ=255)
         atatTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGGTTTTTGGAATTAAAGAAAAagcaagc‑a                                                                                                                              >  2:590156/1‑149 (MQ=255)
           atTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑aata                                                                                                                           <  2:577972/150‑1 (MQ=255)
           atTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGGTTTTTTGGATGAAAAAAAAAGAAAAC‑aata                                                                                                                           >  2:1045329/1‑150 (MQ=255)
           atTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGCTTTTTTGAATGAAAAAAAAAacaagc‑aa                                                                                                                             >  2:715556/1‑148 (MQ=255)
               tACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTTTGGATGAAAAAAAAAgcaaaaaaaaaaa                                                                                                                         >  1:403741/1‑140 (MQ=255)
               tACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGTTCTTTGGAAGAAAAAAAAAAGCAAGG‑aacaaa                                                                                                                         >  2:452064/1‑147 (MQ=255)
                  cAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGGGTTTTTGGATGAAAAAAAAAGGAAAC‑Aaaaaaataa                                                                                                                     >  1:528809/1‑149 (MQ=255)
                        tAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGc                                                                                                              <  2:362385/150‑1 (MQ=255)
                        tAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGGTTTTTGGGATGAAAAAAAAAGCAAAC‑AAAAATAtaaaaa                                                                                                                  >  2:852599/1‑146 (MQ=255)
                         aaGGCGTCTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTTCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCt                                                                                                            <  1:465728/151‑1 (MQ=255)
                              gACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAAttt                                                                                                       <  1:855650/151‑1 (MQ=255)
                                    ccGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGa                                                                                                 <  1:115997/151‑1 (MQ=255)
                                     cGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGaa                                                                                                <  2:120012/151‑1 (MQ=255)
                                        tttATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAAca                                                                                              <  1:1096415/150‑1 (MQ=255)
                                           aTTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAAcatca                                                                                           <  1:120463/150‑1 (MQ=255)
                                           aTTGCGAGTCGCCTTTCCTGATTATAAATCTTATAGTTTGCCTAATCATTTTCCTTTGCATAATGCGGCTGGTATTAATGGCGGGTGCTTTTAGGAATGAAAGACAATGCGAGC‑AATAATATAATAATGGCCTTATTTCTTTGATcat                                                                                             <  1:1019096/148‑1 (MQ=255)
                                              gCGAGTCTCCTTTCCTGATTATAAATCTTATTGGTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTaa                                                                                       <  1:861832/151‑1 (MQ=255)
                                              gCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTa                                                                                        <  2:150933/150‑1 (MQ=255)
                                               cGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAg                                                                                      <  2:535669/151‑1 (MQ=255)
                                                gAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGGTTTTTTGTATTAAAAAAAAAGAAAAC‑AAAAAAATAATAAAAGGCTAATTTTTTTTTAcatcaat                                                                                         >  1:533454/1‑145 (MQ=255)
                                                 aGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCa                                                                                    <  2:462879/151‑1 (MQ=255)
                                                              gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTTTGTATTGAAAAAAAAACAAAC‑AATAAAATAATAAAAGGCTAATTTTTTTTATAATCATTAATCatatta                                                                               >  1:955134/1‑141 (MQ=255)
                                                              gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTc                                                                       <  1:997850/151‑1 (MQ=255)
                                                              gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAAC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGt                                                                        <  2:244921/150‑1 (MQ=255)
                                                                ttATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTTTGAATTAAAAAAAAAACAAAA‑AAAAAAATAATAAAAGCCTAATTTTTTTTTAAATCATTAAAAATATAATCAtcttct                                                                      >  1:960167/1‑150 (MQ=255)
                                                                 tataAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTTTGAATGAAAGAAAAATCAAACAAAAAAAAAAAAAATGGCTTATTTTTTTTTAAAAT‑AATAATAAAATCATCATCATCttt                                                                    >  1:928913/1‑149 (MQ=255)
                                                                 tataAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTa                                                                     <  1:429369/150‑1 (MQ=255)
                                                                   taAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGGTTTTTTGAATGAAAGAAAAAAGAAAC‑AATAAAATAAAAAAAGACTAAATTTTTTTTAAATCATTAAACATATTATCATTCTCTAttt                                                                  >  2:127639/1‑151 (MQ=255)
                                                                    aaaTCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTAttt                                                                  <  1:1110968/150‑1 (MQ=255)
                                                                          tATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTTTGAATGAAAAAAAAAGCAAAC‑AATAATATAATAATGGGCTAATTTTTTTTAAAATCATTAAGCATATCATCATCGTCTAttt                                                                  >  2:101292/1‑144 (MQ=255)
                                                                          tATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGcc                                                               <  2:927059/147‑1 (MQ=255)
                                                                              gTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATcc                                                        <  1:426042/150‑1 (MQ=255)
                                                                              gTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATcc                                                        <  2:881361/150‑1 (MQ=255)
                                                                               tttACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGGTTTTTTGAATGAAAAAAAATGAAAGC‑AAAAAAATAAAAATAGCCTAATTTTTTTTTACATAATTTAGCAAATCATCCTCGTCTATTTTTCAGAATcc                                                        >  2:837488/1‑149 (MQ=255)
                                                                               tttACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGCTTTTTGGGATGAAAAAAAAAGCAAAC‑AAAAAAATAAAAAAGGGCTAATTTTTTTTAACATCATTTAACATATTATCCTCGTTTTTTTTCCAGAATcc                                                        >  2:741647/1‑149 (MQ=255)
                                                                                ttACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTTTGGATGAAAGAAAAAGCAAAA‑AAAAAAATAAAAAAAGCCTAATTTTTTTTAAAAACATTAAACATAAAATCATCGTCTttttttcaagaaccctc                                                     >  2:21784/1‑138 (MQ=255)
                                                                                     tAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTTTGGATGAAAAAAAAAGCAAGC‑AAAAAAATAAAAAATGCCTAATTTTTTTTTACATCATTTAACATATCATCATCGTCTATTTTTCAGAAACCGTCGACtt                                                >  2:433241/1‑151 (MQ=255)
                                                                                      aaTCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGGTTTTTTGTATGAAAAAAAAAGAAAAC‑AAAAAAAAAATAAAAGCCTAATTTTTTTTAACATTATTAAACAAATCATCATCGTCTTTTTTTCAGAATACCTCGACtt                                                >  2:576270/1‑150 (MQ=255)
                                                                                         cATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATaa                                            <  1:1045032/151‑1 (MQ=255)
                                                                                             ttCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGACTTTTTTGAATGAAAAAAAAAAGAAAC‑AAAAATATAAAAAAAGCCTAATTTTTTTTAACATCATTAAACATATCATCATCGTCTATTTTCCCGAAACCGTCGACTTATAAtatc                                        >  2:478731/1‑148 (MQ=255)
                                                                                               ccTTTGCATAATGCGCCTGGGATTAATGGCGGGGGCTTTTTGGAATTAAAAAAAAAGAAAGG‑AAAAATATAATAATGGGGTAATTTTTTTTAACATCATTAAAAAAAAAATCAACGTCTATTTTTCAGAAACCGTCGACCTTTaaacacc                                       >  1:1027596/1‑145 (MQ=255)
                                                                                                         aTGCGCCTGGGATTAATGGCGGGTGCTTTTTTGGAAGAAAAAAAAAACGAGC‑CATAATATAAAAATGGCCTTAATTTTTTTTACATCATTTAACATATCTTCAACATCTTTTTTCCTGAATCCGTCGACTTATAAACTCCAGAAAGGCac                             >  1:1052872/1‑149 (MQ=255)
                                                                                                                                  cTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCtgtg   >  1:394181/1‑151 (MQ=255)
                                                                                                                                   ttttAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTgg  >  1:226105/1‑151 (MQ=255)
                                                                                                                                       |                                                                                                                                                   
GTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGC‑AATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGG  >  NC_000913/4536062‑4536343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑