| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,890 | 0 | C | A | 41.9% | 30.8 / 17.2 | 31 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (12/6); new base A (0/13); total (12/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.42e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAA > NC_000913/357740‑358035 | cTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGcaac > 2:490167/1‑151 (MQ=255) tGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGcaac > 2:527592/1‑150 (MQ=255) gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGcaaca < 2:749787/150‑1 (MQ=255) aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACaaaa < 1:1015633/150‑1 (MQ=255) aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAc < 1:290396/151‑1 (MQ=255) gcgcATCAGGAATTTCAGGCCGCAGACCTGTGTCAGCGTCTCCCGTGTTCCATTTCGGCCAACAC‑CCGCGCATTTATCCACCGCCATGTGCAAAAATGCCTCCGGGGCGG‑CTGTCTGCCGGCTATTTTCCCGCCGCAACAAAAccgccg < 1:69528/149‑1 (MQ=18) cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtc > 2:182004/1‑151 (MQ=255) cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtc > 2:992792/1‑151 (MQ=255) cATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctc > 2:1020948/1‑151 (MQ=255) aTCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctct > 2:913867/1‑151 (MQ=255) cAGCCGCAGACTTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAC‑CCGCGCTTTTACCCAACGCCATGTGAAAAAATGCCTTCGCGGGGG‑CTGTCTGCCAGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTgg < 2:742618/149‑1 (MQ=21) aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑gg > 2:538379/1‑151 (MQ=255) aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑gg > 1:981118/1‑151 (MQ=255) aGCCGCAGACCTGTGTCAGCGCCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAACGCCGTTCTCTCCAGTAGTGGC‑gg > 1:565187/1‑151 (MQ=255) cttgggtCGGCGGCTCCGGTGATTCATTCCCCCCAACAC‑CCGCGCATTTACCAACCGCCATGTGAAAAAATCCTTCCGCGGCGG‑CTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCagtagt < 2:261121/132‑1 (MQ=255) ccTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑gggg > 2:1040925/1‑144 (MQ=255) cggcgcCTCCCGTGATTCTTTCCCCCAAACAC‑CCGCGCTTTTACCAAACCCCTTGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTCCCAGCTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATa < 2:618777/146‑1 (MQ=255) cgaCCGTGATTCATTCCCCCAAACAC‑CCGCGCTTTTACCAAACGCAGTGTGCAAAAATGCCTTCGCGGCGG‑CGGTCTGCCAGTTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTaa < 2:124193/147‑1 (MQ=255) gtgtttaTTTCCCCCCACAACCCGCCCTTTTCCCAACCGCAATTTGAAAAAATCCTTTCGGGGCGG‑CTTTCGGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTc < 1:576754/145‑1 (MQ=255) aTTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCGAGGGG‑AAAAACTTTTAAACCCGCCAttt > 2:710721/1‑147 (MQ=25) tCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGG‑AAGGG‑AAAATGTTTTAATCCCgcctatttt > 2:889254/1‑140 (MQ=25) ccGCCACCAC‑CCGCGCTTTTACCAACCGCCTTGTGAAAAAATGCTTCCCGGGCGG‑CTTTCTGCCAGTTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTtgtg < 2:883859/148‑1 (MQ=255) ccGCCAACAC‑CCGCGCATTTACCCACCGCCATGTGAAAAAATGCCTCCGCGGCGG‑CTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTgg < 2:1052872/151‑1 (MQ=255) ccacaacccGCCCTTTTCTCCACCCCCATGGGAAAAAATGCCTCCGCGGCGG‑TTGTCGGCCGGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGc < 1:24456/142‑1 (MQ=255) accacccGCCCTTTTACCCAACGCCATGTGCAAAACTGCCTCCGCGGCGG‑CTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAAt < 1:722332/145‑1 (MQ=255) aa‑cCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGG‑GAGAG‑AAAAAAATTTTAAGACGTCATGTTGTGTgggcgtct > 1:302348/1‑143 (MQ=25) ccACCGCCTTGTGAAAAAATCCCTCCGGGGCGG‑CTTTCTGCCACTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAt < 2:601488/149‑1 (MQ=255) ccccATGTAAAAAAATGCCTGCGCGGCGG‑CTGTCTCCCAGCTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGaa < 2:1006391/149‑1 (MQ=255) cGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTCCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGaa < 1:494925/151‑1 (MQ=255) tgtgCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGG‑AAGAGAAAAAAACTTTAA‑CCCCCCCTGTTGTGTGGGGAACGCCGCTGGGTACaaaaagggaa > 2:369196/1‑139 (MQ=255) aaaaGGCCTTCGGGGGGGCCTGCCTCCAAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgc < 1:745135/151‑1 (MQ=255) ggggcgg‑cTGTCCCCCAGTTTTTTTCCCGCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGc < 1:866264/148‑1 (MQ=255) gg‑cTGTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCa < 2:244550/149‑1 (MQ=255) tttCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGaa < 1:578633/147‑1 (MQ=255) cacaaaaaAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCaa < 2:499825/149‑1 (MQ=255) aacaaAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAAAAAAATTAAACCCGTCATGTTGTGTTGCGATCGCTGCTGGGGAAAATGTGGGAAGGGGAGGGCGGGGGAGCAACTCCAGAAACACCCTAATTGAGTTCGcc > 1:162129/1‑144 (MQ=255) | CTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGG‑CTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGC‑AAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAA > NC_000913/357740‑358035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |