Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,354,843 G→T intergenic (‑126/+111) lysU ← / ← dtpC lysine tRNA synthetase, inducible/dipeptide and tripeptide permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,354,8430GT98.5% 270.1 / ‑3.1 68intergenic (‑126/+111)lysU/dtpClysine tRNA synthetase, inducible/dipeptide and tripeptide permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (25/42);  minor base . (1/0);  total (26/42)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CCCGTGTTTCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGG  >  NC_000913/4354696‑4354986
                                                                                                                                                   |                                                                                                                                               
cccGTGTTTCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTtata                                                                                                                                              <  1:1109853/151‑1 (MQ=255)
 ccGTGTTTCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTatat                                                                                                                                             <  2:685239/151‑1 (MQ=255)
 ccGTGTTTCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTatat                                                                                                                                             >  2:371210/1‑151 (MQ=255)
 ccGTGTTTCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTATATc                                                                                                                                            >  2:154786/1‑151 (MQ=255)
       ttCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACta                                                                                                                                        <  2:652536/150‑1 (MQ=255)
       ttCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACta                                                                                                                                        <  2:487910/150‑1 (MQ=255)
       ttCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACta                                                                                                                                        <  2:282340/150‑1 (MQ=255)
           tGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACtaat                                                                                                                                      <  1:125091/148‑1 (MQ=255)
               cAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAataaataa                                                                                                                                <  2:825777/150‑1 (MQ=255)
                aGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAAcc                                                                                                                              <  2:101224/151‑1 (MQ=255)
                  aCATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCGTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCAt                                                                                                                            <  1:182184/151‑1 (MQ=255)
                    aTTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCa                                                                                                                          <  2:754752/151‑1 (MQ=255)
                    aTTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCa                                                                                                                          <  2:522167/151‑1 (MQ=255)
                    aTTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCa                                                                                                                          <  2:927474/151‑1 (MQ=255)
                        tGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCaaaa                                                                                                                       <  2:569259/150‑1 (MQ=255)
                             ccTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGttt                                                                                                                 <  2:919600/151‑1 (MQ=255)
                                  aaaTCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTGCACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTGCCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTaaa                                                                                                              <  2:703041/149‑1 (MQ=255)
                                  aaaTCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTaa                                                                                                               <  1:989001/148‑1 (MQ=255)
                                  aaaTCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACACTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTaa                                                                                                               <  1:887812/148‑1 (MQ=255)
                                      ccAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACtt                                                                                                        <  2:596159/151‑1 (MQ=255)
                                        aGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACttat                                                                                                      <  1:472681/151‑1 (MQ=255)
                                         gCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACttatt                                                                                                     >  1:824379/1‑151 (MQ=255)
                                              cAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAAtct                                                                                                >  1:314713/1‑151 (MQ=255)
                                              cAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAAtct                                                                                                <  2:898354/151‑1 (MQ=255)
                                               aaTTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAAtctc                                                                                               >  1:347447/1‑151 (MQ=255)
                                                    aGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGAtt                                                                                           <  1:1077160/150‑1 (MQ=255)
                                                          gATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAgt                                                                                    <  2:617966/151‑1 (MQ=255)
                                                             aaaGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGtt                                                                                 <  2:689168/151‑1 (MQ=255)
                                                             aaaGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGtt                                                                                 >  2:121936/1‑151 (MQ=255)
                                                                  gaCAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATaa                                                                             >  2:943827/1‑150 (MQ=255)
                                                                     aGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTg                                                                         <  1:721974/151‑1 (MQ=255)
                                                                     aGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTg                                                                         <  2:283578/151‑1 (MQ=255)
                                                                      gATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTg                                                                         >  1:420776/1‑150 (MQ=255)
                                                                         cGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGaaa                                                                      <  2:1110019/150‑1 (MQ=255)
                                                                          ggTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGt                                                                    >  1:344982/1‑151 (MQ=255)
                                                                            tACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGtt                                                                   >  2:445943/1‑150 (MQ=255)
                                                                               acTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAAtt                                                               >  2:521793/1‑151 (MQ=255)
                                                                                  gTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAAttt                                                              <  2:531430/149‑1 (MQ=255)
                                                                                    attatCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTc                                                          <  1:833828/151‑1 (MQ=255)
                                                                                      tatCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCa                                                         <  1:445482/150‑1 (MQ=255)
                                                                                      tatCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCa                                                         <  2:1081804/150‑1 (MQ=255)
                                                                                      tatCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCAt                                                        >  1:177367/1‑151 (MQ=255)
                                                                                        tCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATa                                                       >  2:49142/1‑150 (MQ=255)
                                                                                           gCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATaa                                                      <  2:432291/148‑1 (MQ=255)
                                                                                           gCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATa                                                       <  1:1104953/147‑1 (MQ=255)
                                                                                           gCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATa                                                       <  2:783182/147‑1 (MQ=255)
                                                                                           gCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAAcc                                                    >  1:523848/1‑150 (MQ=255)
                                                                                            ccTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCa                                                   >  1:511899/1‑150 (MQ=255)
                                                                                             cTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAAt                                                 >  1:834671/1‑151 (MQ=255)
                                                                                               cGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAAtt                                                <  2:114299/150‑1 (MQ=255)
                                                                                                 aCTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGtt                                             <  1:309036/151‑1 (MQ=255)
                                                                                                   tATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGtttt                                           >  2:619595/1‑151 (MQ=255)
                                                                                                    aTTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGtttt                                           <  1:1107405/150‑1 (MQ=255)
                                                                                                         tAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGa                                      >  1:1009981/1‑150 (MQ=255)
                                                                                                          aaCTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGAc                                     >  1:539993/1‑150 (MQ=255)
                                                                                                             tCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGtt                                 >  2:820450/1‑151 (MQ=255)
                                                                                                              cAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGtt                                 <  1:843009/150‑1 (MQ=255)
                                                                                                               aGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTaa                               <  1:11269/151‑1 (MQ=255)
                                                                                                                   tatcttatcGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCg                            <  2:635280/150‑1 (MQ=255)
                                                                                                                       ttatcGAAACTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGct                       >  2:762152/1‑151 (MQ=255)
                                                                                                                              aaCTAACGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGt                >  2:479290/1‑151 (MQ=255)
                                                                                                                                   aCGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCat           >  2:1047640/1‑151 (MQ=255)
                                                                                                                                    cGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCat           >  1:387648/1‑150 (MQ=255)
                                                                                                                                    cGCTTACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCat           >  2:711539/1‑150 (MQ=255)
                                                                                                                                        tACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATtcg      >  1:230388/1‑151 (MQ=255)
                                                                                                                                        tACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATtc       <  2:517070/150‑1 (MQ=255)
                                                                                                                                        tACCTCAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATtc       <  1:705011/150‑1 (MQ=255)
                                                                                                                                             cAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATtcgtcg   <  2:1049998/149‑1 (MQ=255)
                                                                                                                                             cAAGTTTATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCgg  <  1:474253/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CCCGTGTTTCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGG  >  NC_000913/4354696‑4354986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: