Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,314	A→G	S69P (TCG→CCG)	sapC ←	antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,314	0	A	G	100.0%	260.8 / NA	66	S69P (TCG→CCG)	sapC	antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/35);  total (31/35)

CCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCC  >  NC_000913/1354170‑1354460                                                                                                                                                 |                                                                                                                                                   ccAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATg                                                                                                                                               >  1:624884/1‑150 (MQ=255)  cAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCGCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGGCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGAc                                                                                                                                             <  1:111455/151‑1 (MQ=255)      aCCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGAcggc                                                                                                                                          <  1:85821/150‑1 (MQ=255)         aGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACggcggc                                                                                                                                       >  1:567936/1‑150 (MQ=255)         aGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCa                                                                                                                                      <  2:351897/151‑1 (MQ=255)          ggCCACAGATCGTCGCGGCAAGCTTCACCACAAATGCGCCTCCCCCGGTTGGCGCCGCCCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTGCGCCATAGCGCGGCCATGACGGTGGCa                                                                                                                                      <  1:238941/150‑1 (MQ=255)              acaGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCaataat                                                                                                                                 <  2:517042/151‑1 (MQ=255)              acaGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCaataat                                                                                                                                 >  1:231319/1‑151 (MQ=255)               caGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCaataat                                                                                                                                 <  2:880410/150‑1 (MQ=255)               caGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAAtt                                                                                                                                <  2:920781/151‑1 (MQ=255)               caGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAAtt                                                                                                                                <  2:1069397/151‑1 (MQ=255)                aGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTg                                                                                                                               >  2:649468/1‑151 (MQ=255)                 gATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGa                                                                                                                              <  1:607527/151‑1 (MQ=255)                     gtcgCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAAc                                                                                                                          >  2:689234/1‑151 (MQ=255)                       cgcgGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCg                                                                                                                        >  1:360246/1‑151 (MQ=255)                           gCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGaa                                                                                                                    <  1:1035481/151‑1 (MQ=255)                             aaGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAAt                                                                                                                  <  2:772738/151‑1 (MQ=255)                               gCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAAt                                                                                                                  <  2:27436/149‑1 (MQ=255)                                  tCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTg                                                                                                             >  1:47493/1‑151 (MQ=255)                                        caAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGAt                                                                                                       <  1:778659/151‑1 (MQ=255)                                             gcgcCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGt                                                                                                  <  2:643285/151‑1 (MQ=255)                                             gcgcCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGt                                                                                                  <  1:709529/151‑1 (MQ=255)                                               gcCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTa                                                                                                 >  1:136213/1‑150 (MQ=255)                                                ccacccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTaa                                                                                                <  1:521909/150‑1 (MQ=255)                                                  acccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGgc                                                                                             >  1:297140/1‑151 (MQ=255)                                                   cccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGgcg                                                                                            >  1:785323/1‑151 (MQ=255)                                                        ggTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAc                                                                                       >  2:331569/1‑151 (MQ=255)                                                        ggTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAc                                                                                       >  2:191532/1‑151 (MQ=255)                                                        ggTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAc                                                                                       >  1:938214/1‑151 (MQ=255)                                                          ttGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAcc                                                                                      >  1:822109/1‑150 (MQ=255)                                                            ggCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATATCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAg                                                                                    <  1:626394/150‑1 (MQ=255)                                                                  gCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCa                                                                              >  1:710347/1‑150 (MQ=255)                                                                    tCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCaaa                                                                            >  1:620004/1‑150 (MQ=255)                                                                     ccGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCaaaaa                                                                          <  2:493202/151‑1 (MQ=255)                                                                     ccGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCaaaa                                                                           <  2:480097/150‑1 (MQ=255)                                                                          cAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATaca                                                                      <  1:413778/150‑1 (MQ=255)                                                                           aGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATacac                                                                     >  1:21506/1‑150 (MQ=255)                                                                                     ttAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAcagac                                                            >  1:179120/1‑147 (MQ=255)                                                                                         aaCATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGcccc                                                      <  1:176962/151‑1 (MQ=255)                                                                                              cgcgTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCa                                                 <  1:641635/151‑1 (MQ=255)                                                                                               gcgTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCa                                                 <  1:95225/150‑1 (MQ=255)                                                                                                cgTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGc                                               <  1:750400/151‑1 (MQ=255)                                                                                                 gTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGcc                                              >  2:861123/1‑151 (MQ=255)                                                                                                   ccGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGt                                            >  1:635159/1‑151 (MQ=255)                                                                                                   ccGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGt                                            <  2:551069/151‑1 (MQ=255)                                                                                                      aGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACa                                         <  2:129560/151‑1 (MQ=255)                                                                                                      aGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAAGAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACa                                         <  1:148559/151‑1 (MQ=255)                                                                                                              aGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGAcc                                 >  2:680967/1‑151 (MQ=255)                                                                                                               gTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGAcc                                 >  1:65835/1‑150 (MQ=255)                                                                                                                 ccccAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATc                              <  2:345221/151‑1 (MQ=255)                                                                                                                    cAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCa                           <  1:605722/151‑1 (MQ=255)                                                                                                                      ggAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGc                            >  1:501265/1‑148 (MQ=255)                                                                                                                         agaaagaaACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGc                      >  2:616224/1‑151 (MQ=255)                                                                                                                         agaaagaaACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGc                      >  1:135684/1‑151 (MQ=255)                                                                                                                           aaagaaaCTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCg                     <  1:705519/150‑1 (MQ=255)                                                                                                                           aaagaaaCTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGt                    >  1:275613/1‑151 (MQ=255)                                                                                                                               aaaCTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACt                <  2:854998/151‑1 (MQ=255)                                                                                                                                    tCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATaa            >  1:814624/1‑150 (MQ=255)                                                                                                                                      gCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAAt         <  2:707632/151‑1 (MQ=255)                                                                                                                                      gCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAAt         >  2:811164/1‑151 (MQ=255)                                                                                                                                       ccATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAAtt        >  1:390721/1‑151 (MQ=255)                                                                                                                                        cATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAAttt       >  2:505855/1‑151 (MQ=255)                                                                                                                                         aTAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTg      >  2:386233/1‑151 (MQ=255)                                                                                                                                            gcgcGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTgcgc   <  1:689134/151‑1 (MQ=255)                                                                                                                                            gcgcGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTgcgc   <  2:644982/151‑1 (MQ=255)                                                                                                                                             cgcgGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGcc  <  2:837372/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCC  >  NC_000913/1354170‑1354460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑