Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,660	0	G	T	41.4%	91.5 / 32.7	58	E321* (GAA→TAA)	yeiI	putative kinase
Reads supporting (aligned to +/- strand):  ref base G (5/29);  new base T (24/0);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.85e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCT  >  NC_000913/2252512‑2252810                                                                                                                                                     |                                                                                                                                                           gCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGaa                                                                                                                                                          <  2:692888/151‑1 (MQ=255)   aaCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAgg                                                                                                                                                        <  1:1011128/151‑1 (MQ=255)    aCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGa                                                                                                                                                       <  2:842653/151‑1 (MQ=255)       gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATa                                                                                                                                                     <  1:350377/150‑1 (MQ=255)           gtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGtt                                                                                                                                               <  1:361857/151‑1 (MQ=255)           gtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGt                                                                                                                                                <  2:744769/150‑1 (MQ=255)                    cccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACa                                                                                                                                      <  2:495949/151‑1 (MQ=255)                    cccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACa                                                                                                                                      <  1:886856/151‑1 (MQ=255)                    cccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACa                                                                                                                                      <  1:706270/151‑1 (MQ=255)                    cccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGATTTTTTTTAGGGTA‑AAGTTTTCGCGAc                                                                                                                                       >  2:805131/1‑150 (MQ=255)                       gATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAgcg                                                                                                                                   <  1:299426/151‑1 (MQ=255)                       gATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTTTTTTTAGGGTA‑CAGATTTCCCGCCAgc                                                                                                                                    >  2:626148/1‑150 (MQ=255)                             tCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGGTATAACTTTCTTTAAGGGTA‑AAGTTTTCGCGACAGCG‑ccc                                                                                                                               >  2:79558/1‑148 (MQ=255)                                gTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGTGTAAATATTTTTTTTAGGGGA‑AACTTTCTGCCACAGCG‑cccctctt                                                                                                                          >  1:466085/1‑145 (MQ=255)                                 tttATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGTGTATAGCTTTTTTTTAGGGTA‑AAGTTTTCGCGGCAGCG‑CCCG‑cttt                                                                                                                         >  1:128034/1‑149 (MQ=255)                                 tttATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATTTTGGTTTTTTTTAGGATA‑CAGTTTTCGCGCCAGCG‑CCCG‑cttt                                                                                                                         >  1:531413/1‑149 (MQ=255)                                            gAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGCTTTTTTTTAGGATA‑AAGGTTTCGCGACAGCC‑CCCG‑CTTTTTGGTAGCCTgc                                                                                                             >  1:666111/1‑150 (MQ=255)                                                aGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGGTATAGTTTTTTTTTAGGGTA‑CAGTTTTCGCGGCAGCG‑CCCG‑GTTTTCGGTAg                                                                                                                  >  1:936292/1‑141 (MQ=255)                                                  gATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGATTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCaa                                                                                                      <  2:380093/151‑1 (MQ=255)                                                   aTGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTTGGATATAACTTTTTTTAAGGGAA‑CAGTTTTCCCGACCGCG‑CCCG‑CTTTTCGGTAGCCCGCGCGGcga                                                                                                      >  2:100136/1‑148 (MQ=255)                                                   aTGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTTTTTTTAGGGGA‑AAGTTTTCTCGACAGCG‑CCCG‑CTTTTCGGGAGCCCGCGCgg                                                                                                         >  2:17165/1‑147 (MQ=255)                                                       agaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGGTATAGATTTTTTTGAAGATACCATTTTCCCGGCCAG‑G‑CCCGTTTTTGCGGTGGccgggcgggcattt                                                                                                    >  2:285615/1‑137 (MQ=255)                                                               ttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAACTTTTTTTTAGGATA‑AAGGTTTCCCGACAGCG‑CCCC‑GTTTTCGGTAACCCGCCCGGGAAATTTGCGCGcc                                                                                           >  2:478824/1‑149 (MQ=255)                                                               ttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAACTTTTTTTTAGGATA‑CAGATTTCTCGGCAGCG‑CCCC‑CTTTTTGGTGGCCCGCGCGGCCAtttttcccgcc                                                                                           >  1:14270/1‑141 (MQ=255)                                                                tttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAg                                                                                         <  1:631316/150‑1 (MQ=255)                                                                 ttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGc                                                                                        <  1:169016/150‑1 (MQ=255)                                                                      gACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAg                                                                                   <  2:781421/150‑1 (MQ=255)                                                                          gcgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTTTTTTTAGGGGA‑ACGTTTTCGCGGCAGCG‑CCCG‑GTTTTCGGGAGCCCGCGCGGCCATTTCCCGCGCCacccg                                                                                      >  1:503556/1‑139 (MQ=255)                                                                            gcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATAATGATTTCTTTCAGGATA‑CAGATTCCGCGACAACG‑CCCC‑CTTTTCGGGAGCCTGCGCGGCGATTTTTCGCGCCCGCCGCCG‑CTTtaa                                                                            >  2:964757/1‑150 (MQ=255)                                                                              cAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAaacaa                                                                         <  2:106945/151‑1 (MQ=255)                                                                                  gcATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGATTTTTTTTAGGAAA‑AAGTTTTCCCGACAGCG‑CCCG‑GTTTTTGGGAGCCTGCGCGGGCATTTTTCCCGCCCGCGGGCG‑GTTAAAAAaacc                                                                      >  2:285736/1‑150 (MQ=255)                                                                                      aCGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAaccctaccc                                                                 <  1:66117/151‑1 (MQ=255)                                                                                        gACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAACTTTCTTTTAGGATA‑CAATTTCCGCGACAGCG‑CCCG‑CTTTTCGGGAGCCTGCGCGGCAATTTCTCGCGCCCGCGGCAG‑CTTTAACAaccccaccc                                                                 >  2:155959/1‑149 (MQ=255)                                                                                         aCAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCGGGTGTTTAGCTTTTTTTTAGGGTA‑CAAGTTTCGCGGCACCG‑CCCG‑CTTTGTGGTAGCCTGCGCGGCCATTTCGCGCGCCAGCGGCCG‑CTTTAAAAaacccaccc                                                                 >  1:403446/1‑148 (MQ=255)                                                                                           aGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGtat                                                            <  2:808419/151‑3 (MQ=255)                                                                                                 cAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGAt                                                      <  2:352450/151‑1 (MQ=255)                                                                                                  aGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTGTATAGGTTTTTTTTAGGATA‑CAATTTTCGCGACAGCG‑CCCC‑GTTTGGGGGAGCCCGCGCGGCAATTTTCCGCGCCCGCGGCAG‑CTTTAAAAAACCCACCCCCTCTGCCgc                                                       >  2:25262/1‑148 (MQ=255)                                                                                                  aGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGTATATATATTTTTTTTAGGAGA‑CAGTTTTCGCGGCAGCG‑CCCC‑GCTTTTGGGAGCCTGCGCGGCGAATTTTCGCCCCAGCGGGGG‑CGTTAACAACCCTACCCCGTCTTCCgc                                                       >  1:971945/1‑148 (MQ=255)                                                                                                  aGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGCTTTTTTTAAGGATA‑CAGTTTTCCCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCGATTTTGCGCGCCCGCGGCAG‑CTTAAAAAacccccccc                                                                 >  2:508541/1‑134 (MQ=255)                                                                                                  aGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAAATTTCTTTTAGGATA‑CAGTTTCCCCGCCACCG‑CCCC‑GTTTTTGGTAGCCCGCGCGGGAATTTTTCGCGCCAGCGGCCGCCTTAAAAAaccccccccctctgcc                                                          >  2:458624/1‑135 (MQ=255)                                                                                                   gTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATTGTTTTTTTTTAGGAGA‑AAGTTTTCGCGGCAGCGCCCCG‑TTTTTGGGT‑GCCTGCGCGGCAATTTTGCGCGCCAGCGGCAG‑CTTTAAAAAACCCCCCCCCTCTGCCGCTa                                                     >  1:1014683/1‑150 (MQ=255)                                                                                                        ggTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAAcgcg                                                <  2:376857/150‑1 (MQ=255)                                                                                                           gCTGACGATGGTTTTATGGTGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCtt                                             <  1:898333/150‑1 (MQ=255)                                                                                                           gCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGTAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCttt                                            <  1:688540/151‑1 (MQ=255)                                                                                                              gACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCAt                                         <  2:216732/151‑1 (MQ=255)                                                                                                                 gATGGTTTTATGGCGGGCCTGGGTTATAGCTTTTTTTAAGGATA‑AAATTTCCGCGACAACG‑CCCG‑CTTTTCGGTAGCCTTCGCGGCCATTTTCCCCGCCCGCGGCAG‑CTTAAACAAACCCACCCCGTCTGCCGCTAACGCGCCTTCAtt                                        >  2:168299/1‑149 (MQ=255)                                                                                                                               gggCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTa                         >  1:518266/1‑150 (MQ=255)                                                                                                                                  ccTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACaa                     <  2:666223/151‑1 (MQ=255)                                                                                                                                      gTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGtt                 <  1:340662/151‑1 (MQ=255)                                                                                                                                       tatataGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTg                >  1:822251/1‑151 (MQ=255)                                                                                                                                        atataGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGc               >  2:830926/1‑151 (MQ=255)                                                                                                                                        atataGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGc               <  2:324655/151‑1 (MQ=255)                                                                                                                                         tataGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGcc              >  2:435998/1‑151 (MQ=255)                                                                                                                                          ataGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCg             <  2:685177/151‑1 (MQ=255)                                                                                                                                            aGCTTTCTTGAAGGACA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCCGCGGCAG‑CTTAAACAACCCTTTCCTGTCTGCCGATAACGCGCTTTCATCAGTGCCAATCGTGTAACAATGTTGc               <  1:264891/147‑1 (MQ=255)                                                                                                                                                 tCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCg       <  1:539071/150‑1 (MQ=255)                                                                                                                                                  cTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGAc     >  2:149032/1‑151 (MQ=255)                                                                                                                                                    tGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGAc     <  2:822421/149‑1 (MQ=255)                                                                                                                                                     gAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCt  <  1:680738/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                           GCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATA‑CAGTTTCCGCGACAGCG‑CCCG‑TTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCT  >  NC_000913/2252512‑2252810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑