Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,7020CA42.5% 145.6 / 68.1 113A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base C (51/14);  new base A (0/48);  total (51/62)
Fisher's exact test for biased strand distribution p-value = 1.37e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAA  >  NC_000913/3665555‑3665850
                                                                                                                                                      |                                                                                                                                                       
gatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCaa                                                                                                                                                       >  2:339170/1‑150 (MQ=255)
  tgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCaaaa                                                                                                                                                     >  1:385867/1‑150 (MQ=255)
      cGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑g                                                                                                                                              <  2:774413/151‑1 (MQ=255)
       gAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑gg                                                                                                                                             >  1:576540/1‑151 (MQ=255)
       gAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑gg                                                                                                                                             >  1:309302/1‑151 (MQ=255)
          tAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑Tc                                                                                                                                          >  2:351552/1‑150 (MQ=255)
           aaTGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCa                                                                                                                                         <  1:33786/150‑1 (MQ=255)
           aaTGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCAc                                                                                                                                        >  2:487780/1‑151 (MQ=255)
              gaagaaACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGc                                                                                                                                     >  2:1017311/1‑151 (MQ=255)
                 gaaACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCAt                                                                                                                                  >  2:934347/1‑151 (MQ=255)
                  aaaCTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCAtt                                                                                                                                 >  1:720313/1‑151 (MQ=255)
                  aaaCTTTTTTATGGGCATAAAGATTAAATCCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACAAATAAAAAA‑TTTAC‑AA‑CACACTTATCTACTCGATTTGGGCTTGCACCCGAAAAACCC‑A‑GG‑TCACCGCCAtt                                                                                                                                 <  1:555920/151‑1 (MQ=255)
                   aatttttttATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTTTCCCTTGACACAAATAAAAAA‑TTTTC‑AA‑CCCACTTATCTACTCAATTTTGGCTTGCACCCGCAAAAACC‑A‑GG‑TCACCGCCATTa                                                                                                                                <  1:868423/148‑1 (MQ=255)
                       tttttATGTGCATGAAGATTTAATCCCTCCTCTTTGGGCTTCGGGAGAAATAATAAAAGGGTGTCCTTTGACACAAATAAAAAA‑TTTTC‑AA‑CACACTTATCTCCGCGAATTTGCCTTGCACCCGCAAAACCC‑A‑GG‑TCACCGCCATTaaaa                                                                                                                             <  2:392055/150‑1 (MQ=255)
                            aTGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCg                                                                                                                        >  1:793444/1‑150 (MQ=255)
                             tgtgTATGAAAATTTATTTCCTCCCCCTTGAGCTTCGGGAAAAAAATTGAAAGCTTTTCCTTTCACACAAAAACAAAT‑TTTTC‑AA‑CTCTCTTATCTCCTCAATTTTGGTTTCCACCCGAAAAATCC‑A‑GG‑TCACCGCCATTAAAATAGCGa                                                                                                                       <  2:390541/150‑1 (MQ=255)
                               tgCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGatat                                                                                                                    >  1:986620/1‑151 (MQ=255)
                               tgCATGAAGATTAAATCCCCCCCCCTTGAGCATCGGGAAAAATAATGAAAGCGTTTCCCTTGACACAAATAAAAAA‑TTTTC‑AA‑CACACTTATCTACTCAAATTTGGCTTGCATCCGAAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGata                                                                                                                     <  1:502621/150‑1 (MQ=255)
                               tgCAAGAAGATTTAACCCCTCCTCCTTGAGCAGCGGGAAAAAAAATGAAGGCGTGCCCCTTGACACAAATAAAAAA‑TTTCC‑AA‑CACCCTTTTCTCCTCAAATTTGGCTTGCATCCGAAAAAACC‑A‑GG‑CCACCGCCATTAAAATAGCGata                                                                                                                     <  1:11502/150‑1 (MQ=255)
                                 cATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTc                                                                                                                  >  2:436347/1‑151 (MQ=255)
                                    gAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCAcc                                                                                                               >  1:333216/1‑151 (MQ=255)
                                      aGATTTATTGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTGCCCCTTGACACAAATAAAAAA‑TTTTC‑AA‑CCCCCTTATCTCCCCGATTTTGGCTTGCACCCGAAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCAt                                                                                                             <  2:604007/151‑1 (MQ=255)
                                      aGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCAt                                                                                                             >  2:348470/1‑151 (MQ=255)
                                         tttAATGCCCCCTCCTGGAGCATGGGGAAAAAAAATGAAAGCTTTCCCCTTGACACAAATAAAAAA‑TTTTA‑AA‑CACACTTATCTACTCAAATTTGGTTTCCACCCAAAAAAACC‑G‑GG‑TCACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                            <  1:855873/149‑1 (MQ=255)
                                         tttAATCCCTCCTCCTTGAGCATCGGGAAAAATAATAAAAGCGTGTCCCTTGACACAAATAAAAAA‑TTTTC‑AA‑CACACTTATCTCCTCGAATTTGGCTTCCACCCGAAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                            <  2:44081/149‑1 (MQ=255)
                                          taaaTCCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGATTTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTaa                                                                                                          <  2:77255/148‑1 (MQ=255)
                                           tAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                        >  2:1032435/1‑151 (MQ=255)
                                            aaTGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTAACACGAATACAAAA‑TTTTC‑AA‑CCCATTTATCTACTCGATTTTGGCTTGCATCCGAAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                         <  2:350805/149‑1 (MQ=255)
                                             aTGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACAAATAAAAAT‑TTTTC‑AA‑CACACTTATCTCCTCGATTTTGGCTTGCATCCAAAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCAt                                                                                                      <  2:40174/151‑1 (MQ=255)
                                               gCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                                    >  2:9393/1‑151 (MQ=255)
                                                cctcctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                                    >  1:678367/1‑150 (MQ=255)
                                                cctccccctGGAGCATGGGAAAAAAAAATGAAAGCGTGTCCCTTAACACAAATAAAAAA‑TTTCC‑AA‑CCCACTTATCTACTCAAATTTGGCTTCCA‑CCCCAAAAACCAA‑GG‑TAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                   <  1:89539/151‑1 (MQ=255)
                                                   cctcctTGAGCATGGGGAAAAATAATGAAAGCGTTTCCCTTGACACAAATAAAAAA‑TTTTC‑AA‑CACCTTTATCTACTCGATTTTGCCTTGCACCCGAAAAACCA‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTga                                                                                                 <  1:313492/150‑1 (MQ=255)
                                                    ctcctTGAGCATCGGGAGAAAAAATGAAAGCGTGCCCCTTGACACAAAAAAAAAA‑TTTAC‑AA‑CACACTTATCTACTCAAATTTGGCTTGCATCCGAAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                                    <  1:546916/146‑1 (MQ=255)
                                                         tGAGCATCGGAAGAAAAAATGAAGGCGTCCCCTTAGACACAAATAAAAAT‑TTTAC‑AA‑CCCCTTTATCTCCTCAAATTTGGTTTCCTTCCGAAAAAACC‑G‑GT‑ACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAata                                                                                            <  2:578601/149‑1 (MQ=255)
                                                           accATGGGGAAAAATAAGAAAGGCGTGTCCCTTAACACAAATAAAAAA‑TTTTC‑AA‑CACTCTTATCTACCCGATTTTGGCTGGCATCCGCAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                        <  2:795577/149‑1 (MQ=255)
                                                           aGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                        >  2:354407/1‑151 (MQ=255)
                                                             cATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAttt                                                                                       >  1:584107/1‑150 (MQ=255)
                                                             cATCGGAAAAAATAATGAAAGCGTGCCCTTGGACAC‑AATAAAAAATTTTTC‑AA‑CCCATTTATCTCCGCAAATTTGGCTTTCCTCCGAAAAACCC‑G‑GG‑TAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAttt                                                                                       <  1:685748/150‑1 (MQ=255)
                                                              aTCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTAt                                                                                     >  1:758684/1‑151 (MQ=255)
                                                              aTCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTAt                                                                                     >  1:54682/1‑151 (MQ=255)
                                                              aTCGGAAAAAAAAATGAAAGCGTGCCCCTTGACAAAAATAAAAAA‑TTTAA‑AA‑CACACTTATCTACTCAAATTTGGTTTGCATCCAAAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTAt                                                                                     <  1:126355/151‑1 (MQ=255)
                                                                 gggAAAAATAATGAAGGCGTGTCCCTTGACACAAATAAAAAA‑TTTTC‑AA‑CACACTTATCTACTCAAATTTGGTTTCCACCCGAAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                                    <  2:414638/149‑1 (MQ=255)
                                                                 gggAAAAATAATAAAGGCGTGTCCCTTGACACGAATAAAAAA‑TTTTC‑AA‑CCCACTTATCTCCTCAATTTTGGCTTGCATCCGAAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTc                                                                                  <  2:822347/151‑1 (MQ=255)
                                                                    agaAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTg                                                                               >  1:836251/1‑151 (MQ=255)
                                                                    acaaaaaaTAAAAGCCTTTCCCTTAACCCGAATAAAAAT‑TTTTC‑AA‑CACCTTTTTAAACTCAAATTTGGCTTACATCCCAAAAACCC‑T‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCtt                                                                                <  1:627568/148‑1 (MQ=255)
                                                                    aaaaaaaaTAAAAGCGTGTCCCTTGACACAAATA‑AAAA‑TTTTCAAA‑CCCCCTTATCTCCTCAATTTTGGTTTGCACCCAAAAAACCC‑A‑GG‑ACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTa                                                                                      <  1:726754/142‑1 (MQ=255)
                                                                      aaaTAATGAAAGCGTGTCCCTTGACACAAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCAATTTTGGCTTGCATCCGAAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCa                                                                             <  2:875375/151‑1 (MQ=255)
                                                                          aatGAAGGCGTGCCCCTTGACACGAATAAAAAA‑TTTTC‑AA‑CACACTTATCTACTAAAATTTGGCTTGCATCCGAAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc                                                                          <  1:759419/150‑1 (MQ=255)
                                                                          aatAAAAGCGTGCCCCTTAACACGAATAAAAAA‑TTTTA‑AC‑CACACTTATCTCCTCAATTTTGGCTTGCATCCGAAAAACCC‑A‑GT‑TCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                         <  2:125968/151‑1 (MQ=255)
                                                                           aggaagccctgccccTAGGCACGAACACAAAA‑TTCAC‑AC‑CCCTCTTTTCTCCTCTAATTGGGTTTGCATCCACAAAAACA‑A‑GT‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                           <  2:143870/136‑1 (MQ=255)
                                                                              aaaGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAt                                                                     >  1:1028916/1‑151 (MQ=255)
                                                                                 gggtgtCCCTGACCAAGAAAAC‑AAA‑TTTTC‑AACCACTCTTGTCCCCTCGAATTTGCTTTGCAACCGCAAAAACC‑G‑GC‑TCACCGCCATTAAAATAGCGATATTCACCATTATCCATGGTGAGAATATATTTATGTCTTGCATACGCAAtt                                                                    <  2:240069/147‑1 (MQ=255)
                                                                                  cgcgcccttcgaaaagAATACAAAA‑TTCAC‑AC‑CACCCTGATCTCGCTGAATTTTGCTCGCTCCCAAAAAATCC‑G‑GG‑CCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAg                                                                  <  1:487048/135‑1 (MQ=255)
                                                                                      tCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                             >  1:140846/1‑151 (MQ=255)
                                                                                           tGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                                                         >  2:204203/1‑150 (MQ=255)
                                                                                             acacAAATACAAAT‑TTTTC‑AA‑CACCCTTATCTACTCAATTTTGGTTTGCATCCGCAAAACCC‑A‑GG‑TCGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                                      <  2:615712/151‑1 (MQ=255)
                                                                                             acacAAATAAAAAT‑TTTTC‑AA‑CACACTTATCTCCTCGATTTTGGTTTGCACCCGAAAAAACC‑A‑GT‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCAt                                                       <  1:662773/150‑1 (MQ=255)
                                                                                              cacGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                                     >  1:254864/1‑151 (MQ=255)
                                                                                                    tACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGAt                                               >  1:482189/1‑151 (MQ=255)
                                                                                                     aaaaaa‑TTTCC‑AA‑CACCCTTACCTACTCAAATTTGGCTTACACCCGAAAAACCG‑G‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGAt                                               <  1:122921/148‑1 (MQ=255)
                                                                                                       aaaa‑TTTTC‑AA‑CCCACTTATCTACTCAATTTTGGCTTGCACCCGAAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTg                                             <  1:440344/150‑1 (MQ=255)
                                                                                                        aaa‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGc                                            >  1:32854/1‑150 (MQ=255)
                                                                                                        aaa‑TTTTC‑AA‑CACACTTATCTACTCAAATTTGGCTTCCACCCGAAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCa                                           <  1:419987/151‑1 (MQ=255)
                                                                                                         aa‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                          >  1:303623/1‑151 (MQ=255)
                                                                                                          atttttC‑AA‑CCCCCTTTTCTACTCAAATTTGGTTTGCACCCGAAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                          <  2:779994/149‑1 (MQ=255)
                                                                                                            ttttC‑AA‑CACATTTATCTCCTCGATTTTGGCTTGCATCCAAAAAAACC‑A‑GT‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATa                                         <  2:76513/150‑1 (MQ=255)
                                                                                                               tC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                                     >  2:309221/1‑151 (MQ=255)
                                                                                                                c‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                                     >  1:1045740/1‑150 (MQ=255)
                                                                                                                  aa‑CACACTTATCTACTCGAATTTGGTTTGCATCCGAAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGtt                                      <  1:27315/148‑1 (MQ=255)
                                                                                                                  aa‑CACACTTATCTACTCAAATTTGGCTTACACCCAAAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                                     <  1:355179/149‑1 (MQ=255)
                                                                                                                   a‑cacacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                  >  2:857557/1‑151 (MQ=255)
                                                                                                                       cacTTATCTACTCGATTTTGGCTTGCATCCAAAAAAACC‑G‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                  <  2:567210/148‑1 (MQ=255)
                                                                                                                        acTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAat                              >  2:616553/1‑151 (MQ=255)
                                                                                                                          ttATCTACTCAAATTTGGTTTGCACCCAAAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                             <  1:899278/150‑1 (MQ=255)
                                                                                                                           tATCTACTCAAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                             <  2:914420/149‑1 (MQ=255)
                                                                                                                             tCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTa                          >  1:586769/1‑150 (MQ=255)
                                                                                                                              ctactaAAATTTGGCTTACACCCGAAAAACCA‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                               <  1:813948/144‑1 (MQ=255)
                                                                                                                               tACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                       >  2:1041463/1‑151 (MQ=255)
                                                                                                                                aCTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                       >  1:464415/1‑150 (MQ=255)
                                                                                                                                aCTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAATCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                       >  1:274061/1‑150 (MQ=255)
                                                                                                                                 cTCGAATTTGGCTTGCACCCACAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                       <  2:120167/149‑1 (MQ=255)
                                                                                                                                  tcaaaTTTGGCTTGCTTCCGAAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTAc                         <  1:348404/143‑1 (MQ=255)
                                                                                                                                  tCGATTTTGGCTTGCATCCAAAAAACCC‑A‑GG‑TCACCGCCGTTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTAc                         <  2:85561/146‑1 (MQ=255)
                                                                                                                                  tCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaa                     >  2:117615/1‑150 (MQ=255)
                                                                                                                                   caaaTTTGGCTTGCACCCGAAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCACGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaaa                    <  2:216084/148‑1 (MQ=255)
                                                                                                                                   cGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAAc                   >  1:931671/1‑151 (MQ=255)
                                                                                                                                    gAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAAc                   >  2:331577/1‑150 (MQ=255)
                                                                                                                                     aaTTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat                 >  1:352/1‑151 (MQ=255)
                                                                                                                                       tttGGCTTGCATCCGAAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACata                <  1:383213/150‑1 (MQ=255)
                                                                                                                                       tttGGCTTGCACCCGCAAAAACC‑A‑GT‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACata                <  2:581905/150‑1 (MQ=255)
                                                                                                                                          ggtttGCATCCGAAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATAtt              <  1:572018/146‑1 (MQ=255)
                                                                                                                                            cTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTg          >  1:546140/1‑151 (MQ=255)
                                                                                                                                             ttccacccGAAAAACCC‑A‑GG‑CCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTg          <  1:414586/144‑1 (MQ=255)
                                                                                                                                              ggCATCCGCAAAAAGC‑G‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGtt        >  1:95356/2‑151 (MQ=255)
                                                                                                                                                 acccGAAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGt         <  1:339113/145‑1 (MQ=255)
                                                                                                                                                   ccGAAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttatta   <  2:740364/151‑1 (MQ=255)
                                                                                                                                                   ccGAAAAACCC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTtta      <  1:398366/148‑1 (MQ=255)
                                                                                                                                                    cccAAAAACC‑AGGG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttatta   <  1:121518/149‑1 (MQ=255)
                                                                                                                                                     ccAAAAACC‑C‑GGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTaa  <  1:904548/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
GATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAA‑TTTTC‑AA‑CACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACC‑A‑GG‑TCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAA  >  NC_000913/3665555‑3665850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑