Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	907,602	0	A	C	40.4%	98.9 / 10.4	52	G227G (GGT→GGG)	ybjT	putative NAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (27/4);  new base C (0/21);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 6.59e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGGTGC  >  NC_000913/907454‑907750                                                                                                                                                     |                                                                                                                                                     gTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTAcc                                                                                                                                                    >  2:571076/1‑151 (MQ=255)    gcgcGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCt                                                                                                                                                 <  1:1037595/151‑1 (MQ=255)    gcgcGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCt                                                                                                                                                 <  1:159596/151‑1 (MQ=255)      gcgGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTc                                                                                                                                                <  2:648124/150‑1 (MQ=255)              atcCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCAt                                                                                                                                        >  1:899522/1‑150 (MQ=255)                    cagcagATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGt                                                                                                                                 >  2:888121/1‑151 (MQ=255)                               gtTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTg                                                                                                                      >  2:448003/1‑151 (MQ=255)                                tttCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGc                                                                                                                     >  2:622834/1‑151 (MQ=255)                                 ttCAGCCCCTGAATCACCGCCCGGGGGGTGGGGGGCGGTACGGAAGTAATCACATTGAAAACCCACCCCAAAACCCAGCGGGGGGGGAGGGGGAGGGGGATCACCCGGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGc                                                                                                                     <  2:589279/150‑1 (MQ=255)                                  tCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTg                                                                                                                   >  1:445822/1‑151 (MQ=255)                                    aGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGAt                                                                                                                 >  2:47158/1‑151 (MQ=255)                                    aGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGAt                                                                                                                 >  2:460412/1‑151 (MQ=255)                                          tGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTg                                                                                                            >  1:508048/1‑150 (MQ=255)                                          tGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTg                                                                                                            >  2:816802/1‑150 (MQ=255)                                             aTCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGCGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGc                                                                                                         >  2:704593/1‑150 (MQ=255)                                                accGCCCGGGCGGTGGTGGGCGGTACGAAAGTAATCACATTGAGAAACCACACCGAAACCCAGGGGGTGGGGGGGGGGATGGGGATCAACCGGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCa                                                                                                        <  2:79095/146‑1 (MQ=255)                                                aaCGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACct                                                                                                     >  1:733625/1‑151 (MQ=255)                                                  cGCCCTGGCGGTGGTGGGGGGTGCGGATGTATTCATATAGACAACCCACACCAAAATCCGGCGGGGGGGGGGGGGGAGGGGGATCAACCAGGGGGTCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACctc                                                                                                    <  2:821655/150‑1 (MQ=255)                                                            gtggggGGCGAGAGGGAAGTAATCACATTGAGAAACCCCCCCGAAACCCAGCGGGTGGGGAGGGGGATGGGGACCAACCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcggc                                                                                           <  1:1021587/149‑1 (MQ=255)                                                              gggggggggaagggAAGTAACCACATTGAGAACCCCCACCAAAACCCAGCGGGTGGGGGGGGGGAGGGGGATCAACCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCt                                                                                       <  1:415489/139‑1 (MQ=255)                                                              gggggggggTACGGAAGTAATCACATTGAGAACCCCCCCCAAAATCCGGGGGGGGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCggcggc                                                                                        <  2:136436/147‑1 (MQ=255)                                                               gtggGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCtt                                                                                      >  2:1008150/1‑151 (MQ=255)                                                                 gggCGGTAGGGAAGAAACCACATTGAGAACCCCCCCCGAAATCCGGGGGGTGGGGGGGGGGATGGGGATCAACCGGGGGCGCTCCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTc                                                                                     <  1:715319/150‑1 (MQ=255)                                                                     ggTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGa                                                                                   >  2:964139/1‑148 (MQ=255)                                                                       tagggAAGTAATCACATGGAAAAACCCCCCCAAAACCCGGGGGGGGGGGGGGGGGATGGGGATCAACCAGCGGCTCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGAt                                                                               <  1:227411/147‑1 (MQ=255)                                                                       taggaaaGAAATCCAATTGAAAACCCACACCAAAACCCGGCGGGTGGGGGGGGGGATGGGAAGAAACCGGGGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGAt                                                                               <  1:712816/145‑1 (MQ=255)                                                                       tACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATg                                                                              >  1:53742/1‑151 (MQ=255)                                                                          ggAAGTAATCACTTTGAGAACCCCCACCGAACCCCGGGGGGTGGGGAGGGGGATGGGGATCAACCGGCGGCGCTTCCCCCTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCg                                                                            <  1:618161/150‑1 (MQ=255)                                                                            aataaaTCACATTGAAAACCCCCACCGAAACCCGGGGGGTGGGGGGGGGGAGGGGGATCACCCGGGGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCg                                                                            <  1:572919/144‑1 (MQ=255)                                                                            aaGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGgtg                                                                         >  2:931897/1‑151 (MQ=255)                                                                                aagtactttGACAACCCCCACCAAAACCGAGAGGGTGGGAAGGGGGTTGGGCATCACCGAGCGCCCCTCCCCGCTCTCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGtt                                                                       <  1:873944/142‑1 (MQ=255)                                                                                  tCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCTCCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCt                                                                   >  2:660833/1‑151 (MQ=255)                                                                                    acaTTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTgg                                                                 >  2:105586/1‑151 (MQ=255)                                                                                       ttGAAAACCCACACCAAAACCCAGGGGGGGGGGGGGGGGATGGGGATCACCCGGCGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGcc                                                               <  2:709592/150‑1 (MQ=255)                                                                                         gaaaaccccccccGAAACCCGGGGGGTGGGGGGGGGGAGGGGGATCACCCAGGGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCgg                                                             <  1:429172/139‑1 (MQ=255)                                                                                          agaAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGgat                                                           >  1:9163/1‑151 (MQ=255)                                                                                                  cacCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTaac                                                   >  1:68774/1‑151 (MQ=255)                                                                                                      gAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACg                                               >  2:1020020/1‑151 (MQ=255)                                                                                                       aactCCAGGGGGTGGGGGGGGGGATGGGGATCAACGGGCGGCGCTTCCCGCGCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTaacaac                                                <  1:276999/146‑1 (MQ=255)                                                                                                           ccAGGGGGGGGGGGGGGGGAGGGGAATCACCCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAAcc                                          <  2:443182/151‑1 (MQ=255)                                                                                                               cGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAAcc                                          >  2:858709/1‑147 (MQ=255)                                                                                                                gggTGGGGGGGGGGATGGGGATCAACCAGCGGCCCTTCCCCCTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAAcc                                          <  1:241076/146‑1 (MQ=255)                                                                                                                gggTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGa                                       >  1:826401/1‑149 (MQ=255)                                                                                                                 gggggggggggggATGGGGATCAACCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAg                                    <  2:188409/148‑1 (MQ=255)                                                                                                                             gatggggatCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGttt                        >  2:761184/1‑151 (MQ=255)                                                                                                                              atggggatCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGtttt                       >  1:524845/1‑151 (MQ=255)                                                                                                                                 gggatCACCCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCa                    <  1:47151/151‑1 (MQ=255)                                                                                                                                 gggatCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTcc                     >  1:390905/1‑150 (MQ=255)                                                                                                                                  gaacaaccccgggccgcTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAg                   <  1:447921/137‑1 (MQ=255)                                                                                                                                            gcggccCTTCCCGCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGc         <  1:409769/151‑1 (MQ=255)                                                                                                                                              ggcgCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCg        <  2:905818/150‑1 (MQ=255)                                                                                                                                                   ttACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGGTGc  >  2:628000/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGGTGC  >  NC_000913/907454‑907750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑