Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 993,913 | 0 | G | C | 44.4% | 80.0 / 18.6 | 54 | G44G (GGC→GGG) | ssuB | aliphatic sulfonate ABC transporter ATPase |
Reads supporting (aligned to +/- strand): ref base G (24/6); new base C (0/24); total (24/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.09e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAAT > NC_000913/993763‑994063 | ccACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCgg > 1:844155/1‑151 (MQ=255) ccACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCgg > 2:994892/1‑151 (MQ=255) cACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGc > 1:103230/1‑151 (MQ=255) aCGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGcc > 1:166635/1‑151 (MQ=255) gTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCccac > 1:344386/1‑151 (MQ=255) aGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCccacc > 2:625289/1‑150 (MQ=255) gACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGc > 2:325112/1‑151 (MQ=255) gcgcATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCca < 1:706198/150‑1 (MQ=255) cATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACaa > 2:491338/1‑150 (MQ=255) cTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTg > 1:132696/1‑150 (MQ=255) gAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGAcc > 1:655853/1‑151 (MQ=255) aCATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGc > 1:326731/1‑151 (MQ=255) tttctcCCTGATTTCCAGCGGGGGGTGGGGCCCCCGCAAACCCCCCCCCGGGGGTGGGCTTTCCCAGCCCGGCCAGCAGGCGCAGCAGGGTACTTTCC‑CCACCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTa < 1:923948/148‑1 (MQ=255) tCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATcc > 1:127687/1‑150 (MQ=255) ccTGATTTTCAGCCGGCGGTGGGGGCCCCGCAAACCCTCCGCCTGGGTTGGGCTTTTCCAGCCCTCCCAGCGGGCGCAGCAGGTTATTTTTC‑CCCCCACCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATc < 2:762537/148‑1 (MQ=255) ttccagccgggGGTGGGGTCCCCGCAAACCCACCGCCGGGGGTGGGCGTTCCCAGCCCGGCCAGCAGGCGCAGCAGGGTTTTTTTC‑CCCCCCCCGCTGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTgg < 2:650254/146‑1 (MQ=255) cagccgggGGTGTGGTGCCGCCAACCACACGGTGTGCGTTTGGCTTTCCCACCCCCCCAAGCGGGCCAAGCGGGTTCTTTTTC‑CCACCCCCCCTGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAg < 2:673359/151‑1 (MQ=255) agccagcGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAgg > 2:878056/1‑151 (MQ=255) ccggggttggggtcCCCGCAACCCCACCGCCGGCGGTGGGCTTTCCCAGCCCTCCCAGCAGGCGCAGCGGGTTTCTTTTC‑CCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAg < 2:100225/138‑1 (MQ=255) ggTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGatat > 2:1028333/1‑151 (MQ=255) gtggtgCCCGCTAACACATCGCCGGGGTTTGGCTTTCCCAGCCCGCCCAGCAGGCGCAGGGGGGT‑CTTTTGCCCCCCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATAttt < 2:432138/150‑1 (MQ=255) ggcccccGCAAACACTCCGCCTGCGTTGGGCTTTCCCACCCCGCCCAGCGGGCGCAGCAGGTTTTTTTTG‑CCCCCCCCGCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATAttt < 1:179258/144‑1 (MQ=255) cGCTACCACATCGCCGGCGGTTGGCGTTCCCAGCCCTGCCAGCGGGCGCAGCAGGGTCTTTTTG‑CCCCCCCCGCTGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTaa < 2:327784/151‑1 (MQ=255) accactcccccgggggtgggtgTTCCCACCCCGGCCAGCGGGCCCAGCAGGGTTTTTTTC‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGtt < 1:1043936/137‑1 (MQ=255) caccGCCTGGGGTGGGCTTTCCCAGCCCGGCCAGCGGGCGCAGCAGGGTATTTTTG‑CCACCCCCGCTGCGGCCTACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGttt < 2:89784/145‑1 (MQ=255) ccccggggtttGGCGTTCCCGGACCTGCCAGCGGGCGCGGCGGGGTTTTTTTC‑CCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTa < 2:574052/141‑1 (MQ=255) cGCCGGGGGTGGGCTTTCCCAGCCCGGCCAGCAGGCGCGGCAGGTTCTTTTTC‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCtt < 2:300685/149‑1 (MQ=255) gggtttGGCGTTCCCAGCCCGGCCAGCGGGCGCGGCAGGGTCCTTTTG‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGc < 2:282478/145‑1 (MQ=255) gtttGGCTTTCCGAGCCCGGCCAGCGGGCGCAGCGGGTTATTTTCC‑CCCCCCCCGTTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACt < 2:169315/143‑1 (MQ=255) ggTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTg > 1:686460/1‑151 (MQ=255) cccaccccggCCAGCAGGCGCAGCGGGTTTCTTTTC‑CCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGa < 1:140841/133‑1 (MQ=255) ccAGCCCTGCCAGAGGGCGCAGCGGGTTTTTTTTC‑CCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATg < 2:399329/150‑1 (MQ=255) cccgCCAGCGGCCGCGGGGGGGTTTTTTTC‑CCCCCCCCGCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGc < 1:369816/148‑1 (MQ=255) cTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGcc > 2:978121/1‑151 (MQ=255) cTGCAAGCGGGCGCGGAAGGTTATTTTGC‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCg < 2:143130/147‑1 (MQ=255) ccAGCAGGCGCAGCAGGTTATTTTTG‑CCCCCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCt < 2:166664/150‑1 (MQ=255) cagcggGCGCAGCGGGTTCTTTTTC‑CCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTg < 1:778617/150‑1 (MQ=255) agGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTc > 1:876511/1‑150 (MQ=255) agGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTc > 2:522338/1‑150 (MQ=255) ggCGCAGCAGGGTTTTTTCG‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAg < 2:81561/151‑1 (MQ=255) ggCGCAGCAGGGTCTTTTTC‑CCCCCCCCGCGGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCa < 2:30009/150‑1 (MQ=255) ggCGCAGCAGGGTCTTTTCC‑CCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAg < 2:122948/151‑1 (MQ=255) cgcagggggttctttttC‑CCACCCCCGCTGCGCCCCACCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGa < 1:267277/137‑1 (MQ=255) gcagcaGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg > 1:146347/1‑151 (MQ=255) caccaggttttttttC‑CCCCCCCCGCTGCGCCCCCCCTCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTc < 1:625179/134‑1 (MQ=255) gcagattACTTTTC‑CCACCACCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGa < 2:132716/140‑1 (MQ=255) gggttctttttG‑CCCCCCCCGCGGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCa < 2:806137/143‑1 (MQ=255) ggTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGt > 1:585674/1‑150 (MQ=255) gTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAt > 2:264974/1‑151 (MQ=255) cttttccccccccccGCTGCCCCCCCCCGCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCa < 1:221492/132‑1 (MQ=255) tttG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCAt > 2:940520/1‑150 (MQ=255) ccaccGCGCCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGc < 1:976893/151‑1 (MQ=255) accGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcct > 2:810549/1‑151 (MQ=255) ccGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctc > 2:1022357/1‑151 (MQ=255) cGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctcc > 1:656718/1‑151 (MQ=255) tGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTc > 2:392593/1‑151 (MQ=255) gcgcCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctcc < 2:90673/147‑1 (MQ=255) ggCCCACCACCGCCACAAACCGCCCTGCCATAATAAGTAAAACGAGTTTGTGCACGACGATATTCTCGGCGTAATGTCTGCCTGCTGCGTTGAGCAACAATGTCCTTCACTGCTTCAGACGAGCAGTATTCACACCGAGGCCTCCTTc > 1:653249/1‑148 (MQ=255) gCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAAt > 2:121312/1‑151 (MQ=255) | CCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTG‑CCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAAT > NC_000913/993763‑994063 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |