Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,314	A→G	S69P (TCG→CCG)	sapC ←	antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,314	0	A	G	100.0%	213.6 / NA	55	S69P (TCG→CCG)	sapC	antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/26);  total (29/26)

GTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCG  >  NC_000913/1354166‑1354458                                                                                                                                                     |                                                                                                                                                 gTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGcc                                                                                                                                                <  2:532257/151‑1 (MQ=255)  tGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGcc                                                                                                                                                >  1:810623/1‑150 (MQ=255)   gCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCAt                                                                                                                                              >  2:570602/1‑151 (MQ=255)     ccAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATg                                                                                                                                             >  1:1014772/1‑150 (MQ=255)       aGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGAcg                                                                                                                                          <  2:249949/151‑1 (MQ=255)       aGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGAcg                                                                                                                                          >  2:143757/1‑151 (MQ=255)           ccAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGAcggcgg                                                                                                                                      <  2:905951/151‑1 (MQ=255)            cAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACggcggc                                                                                                                                     >  2:932887/1‑151 (MQ=255)             aGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACggcggc                                                                                                                                     >  1:60292/1‑150 (MQ=255)                 cacaGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCaata                                                                                                                                 >  2:356985/1‑150 (MQ=255)                   caGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAAtt                                                                                                                              <  2:994450/151‑1 (MQ=255)                              ggCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCgaga                                                                                                                   <  1:626286/151‑1 (MQ=255)                              ggCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCg                                                                                                                      >  1:888580/1‑148 (MQ=255)                                  aGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAAtt                                                                                                               <  1:386724/151‑1 (MQ=255)                                  aGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAAtt                                                                                                               <  1:558779/151‑1 (MQ=255)                                         ccacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTc                                                                                                        <  2:215802/151‑1 (MQ=255)                                          cacaAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCg                                                                                                       <  1:882201/151‑1 (MQ=255)                                           acaAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCg                                                                                                       >  2:26270/1‑150 (MQ=255)                                                  cgcCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTa                                                                                               <  2:398549/151‑1 (MQ=255)                                                     cacccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAgg                                                                                            >  1:58522/1‑151 (MQ=255)                                                           cGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCaaa                                                                                      <  1:1051434/151‑1 (MQ=255)                                                             gTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAcc                                                                                    <  2:761971/151‑1 (MQ=255)                                                             gTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAcc                                                                                    >  1:808370/1‑151 (MQ=255)                                                                 gcgcAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATACCCGAGAAATTGCTGGTCGAAGCCGTAAGGCGCAAACCAgcc                                                                                <  1:31037/151‑1 (MQ=255)                                                                 gcgcAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAgcc                                                                                <  2:818491/151‑1 (MQ=255)                                                                     aGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCa                                                                            <  2:4305/151‑1 (MQ=255)                                                                            cTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATac                                                                     >  1:962675/1‑151 (MQ=255)                                                                              cAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATacac                                                                   >  1:973887/1‑151 (MQ=255)                                                                                gTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATacac                                                                   >  1:155493/1‑149 (MQ=255)                                                                                  aaaCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTa                                                               <  1:182865/151‑1 (MQ=255)                                                                                   aaCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAc                                                              <  2:485413/151‑1 (MQ=255)                                                                                    aCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAca                                                             <  2:701834/151‑1 (MQ=255)                                                                                    aCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAc                                                              >  2:930779/1‑150 (MQ=255)                                                                                        cTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAcagcc                                                          >  2:796943/1‑150 (MQ=255)                                                                                          tAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACGTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAcagccag                                                        <  1:256858/150‑1 (MQ=255)                                                                                           aaaaCATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACagccagcc                                                      <  2:77247/151‑1 (MQ=255)                                                                                             aaCATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGcccc                                                    >  1:687541/1‑151 (MQ=255)                                                                                                  cgcgTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCa                                               <  2:619441/151‑1 (MQ=255)                                                                                                    cgTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGc                                             >  2:4339/1‑151 (MQ=255)                                                                                                           ggTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTAc                                        >  1:534532/1‑149 (MQ=255)                                                                                                            gtcgtcAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAgg                                     <  2:659479/151‑1 (MQ=255)                                                                                                              cgtcAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGc                                    <  2:148243/150‑1 (MQ=255)                                                                                                                 cAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGa                                 <  2:647032/150‑1 (MQ=255)                                                                                                                 cAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGAc                                >  1:895515/1‑151 (MQ=255)                                                                                                                     ccccAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATc                            >  1:1010821/1‑151 (MQ=255)                                                                                                                     ccccAGGAAGAAAGAAACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGAAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATc                            >  2:822754/1‑151 (MQ=255)                                                                                                                             agaaagaaACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGc                    >  2:603162/1‑151 (MQ=255)                                                                                                                             agaaagaaACTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGc                    >  2:815052/1‑151 (MQ=255)                                                                                                                                   aaaCTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACt              >  1:884952/1‑151 (MQ=255)                                                                                                                                   aaaCTTCGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACt              >  1:853191/1‑151 (MQ=255)                                                                                                                                         cGCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATaa          <  2:980434/149‑1 (MQ=255)                                                                                                                                          gCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAAt       >  2:893558/1‑151 (MQ=255)                                                                                                                                          gCCATAGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGCCACTATAAAAt       >  1:881069/1‑151 (MQ=255)                                                                                                                                               aGCGCGGCCATGACGGCGGCAATAATTGATACCCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTgc   <  2:624123/150‑1 (MQ=255)                                                                                                                                               aGCGCGGCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTgcg  >  2:554312/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCG  >  NC_000913/1354166‑1354458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑