Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	925,912	0	G	C	43.5%	122.0 / 29.4	70	noncoding (60/88 nt)	serW	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (12/27);  new base C (30/0);  total (43/27)
Fisher's exact test for biased strand distribution p-value = 3.59e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAAT  >  NC_000913/925763‑926062                                                                                                                                                       |                                                                                                                                                          gTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGa                                                                                                                                                          <  2:1107223/151‑1 (MQ=255)        cAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGATT‑GAAACCCCGATACgct                                                                                                                                                    >  1:588419/1‑148 (MQ=37)        cAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑GAACCCCCCATACGtt                                                                                                                                                    >  1:967332/1‑150 (MQ=255)         aGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTg                                                                                                                                                   <  1:1010489/150‑1 (MQ=255)          gCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGcc                                                                                                                                                 <  1:538591/151‑1 (MQ=255)            ttAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGTTT‑GGAACCCCGAAACGGTGcc                                                                                                                                                 >  1:517092/1‑149 (MQ=37)                         ggTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑GAAACCCCCATACGTTTCCGTATaca                                                                                                                                          >  2:463507/1‑143 (MQ=255)                          gTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑GAACCCCCCATACGTTGCCGGATACAC‑A‑Cttt                                                                                                                                  >  1:1063341/1‑148 (MQ=255)                          gTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTC‑GAACCCCCCATACGTTGCCGGATAAAC‑A‑Cttt                                                                                                                                  >  1:1082644/1‑148 (MQ=255)                             aCATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGc                                                                                                                            <  2:258868/151‑1 (MQ=255)                               aTACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCTGAACCCCC‑ATACGTTGCCggttaacaccttt                                                                                                                                    >  2:526016/1‑131 (MQ=21)                                 aCTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑GAAACCCCCCTACGTTGCCGTATAAAC‑A‑CCTTTCAGGCGTg                                                                                                                         >  2:1029093/1‑150 (MQ=21)                                   tCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGGTT‑TAAACCCCCAAACGGTGGCGGATACAA‑ACCTTTccgg                                                                                                                              >  2:297529/1‑142 (MQ=21)                                      aCCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑GAAACCCCCGTACGTTTCCCTATACAC‑A‑CCTTTCAGGCGTGGTcc                                                                                                                     >  2:819158/1‑149 (MQ=21)                                       cccGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTc                                                                                                                  <  1:973608/151‑1 (MQ=255)                                         cGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTT‑GAACCCCCCATACGTTTCCGGATACAC‑A‑CCTTTCAGGCGGGGTCCtt                                                                                                                   >  2:997312/1‑148 (MQ=18)                                           cccACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGcc                                                                                                              <  1:466086/151‑1 (MQ=255)                                                     tCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGaca                                                                                                     <  2:632910/150‑1 (MQ=255)                                                          gCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCa                                                                                                <  1:981280/150‑1 (MQ=255)                                                            cGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAAGGGGGGGGTT‑GTACCCCCCCTACGTTGTCGGAAACAA‑C‑CATTCCCGGCGGGCTCCTTTAGTCAcacacacaacacccc                                                                                              >  1:885529/1‑136 (MQ=12)                                                               acagacagATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCaaa                                                                                          <  1:124054/150‑1 (MQ=255)                                                                cagacagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTT‑GGAACCCCCATACGTAGCCGTATAACC‑A‑CTTTCCCTGCGGGCACCCTCACCCACACCGCCAACCCA‑CCaaaat                                                                                        >  1:433757/1‑149 (MQ=9)                                                                    cagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGGGGGGGGGGTTT‑GAACCCCCCAAACGTTGCCCGATACACAA‑CTTTCC‑GGCGGGCTCCTTCAGCCCCCCCGACACCTCA‑CCCAAATtttt                                                                                    >  2:1080771/1‑151 (MQ=14)                                                                    cagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGTTT‑GAACCCCCCCTACGTTGCCGTATACAC‑A‑CTTTTCCGGCGTGCTCCTTCAGCCACTCCGGCACCTCA‑CCCAAATtttt                                                                                    >  2:958157/1‑151 (MQ=14)                                                                     agaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGtttt                                                                                   <  2:1003108/151‑1 (MQ=255)                                                                     agaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGAGTC‑TAACCCCCCCTACGGTGCCGGATAAAC‑A‑CTTTCCCGGCGTGCTCCTTCTGCCACCCCGGCAACCCC‑CCAAAATGtttt                                                                                   >  1:37393/1‑151 (MQ=14)                                                                          aaaTGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTT‑GAACCCCCGCTACGTTGCCGTATACAC‑A‑ATTTCCAGGCGTGCTCCCTCAGCCCCCCGGACACCCCA‑CCAAAATGTTTTTCt                                                                                >  1:438931/1‑149 (MQ=14)                                                                            aTGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTT‑GAAACCCCCATACGTTGCCGTATAAAC‑A‑CTTTTCCGGCGTGCTCCTTCAGCCACCCGGACACCCCA‑CCAAAATTTTTTTTTAcc                                                                             >  1:487053/1‑150 (MQ=11)                                                                            aTGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCa                                                                            <  1:56749/151‑1 (MQ=255)                                                                              gCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTT‑GAACCCCCCATACGTTTCCGGATACAC‑A‑CTTTTCAGGCGTGGTCCTTCAGCCACTCCGGCACCCCA‑CCAAAATGttttttttccca                                                                           >  1:29146/1‑142 (MQ=11)                                                                              gCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCaaa                                                                          <  1:1033999/151‑1 (MQ=255)                                                                                  aaaaaaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑GAAACCCCCATACGTTGCCGTATAAAC‑A‑CTTTCCAGGCGGGCTCCTTCCGCCACTCCGACACCCCC‑CCCAAATGTTTTTCTTACaaaac                                                                        >  1:1134019/1‑147 (MQ=11)                                                                                      aaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCGAGGGGGGGGTTC‑GAAACCCCCATACGCTTCCGTATACAC‑A‑CTTTCCCGGCGGGCTCCTTCAACCACTCCGACACCCCA‑CCAAAATTTTTTTTTACCCAACCCCATgg                                                                  >  1:278188/1‑151 (MQ=14)                                                                                       aaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑GAACCCCCCATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACCCGGACACCCCA‑CCAAATTGTTTTTCTACCAAACCTCATggg                                                                 >  1:397699/1‑151 (MQ=255)                                                                                            cGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGTGGGGGGGGTTT‑CAACCCCCCCTAAGTTGCCGTATAAAC‑A‑CTTTTCAGGCGTGGTTCTTCCCCCACTCCGGCACCCCA‑CCCAAATTTTTTTCTATCAAAACTCATtggtggg                                                             >  2:218434/1‑149 (MQ=9)                                                                                            cGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAATCCTCATGGGTGGCa                                                            <  2:1105204/151‑1 (MQ=255)                                                                                             gCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCaa                                                           <  2:526700/151‑1 (MQ=255)                                                                                              cACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTTAGGGGGGGCTTT‑GAACCCCCCGTACCTTGCCGTATACCC‑C‑ATTTCCCCGGGGGGTCCTTCACCCACACGG‑CACCCCACCCCCAATGCTTTGCTTCCACACCCCATGGGGGGCaa                                                           >  1:822697/1‑150 (MQ=2)                                                                                              cACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCTAAGGGGGGGTTC‑TAACCCCCCCTTAGTTGCCGTATAAAA‑A‑CTTTCCAGGCGTGCTCCCTCAGCCCCCCGGACACCCCA‑CCCAAATTTTTTTCTACCAAAACCCCTGGGGGGcga                                                           >  2:647035/1‑148 (MQ=2)                                                                                               aCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑CGATCCCCCACACGGTGTCGTACAAAC‑C‑CCTTCCAAGCGGGGTCCCTCAGCCACTCCCGCCCCTCA‑ACAAATTTTTTTTCTTCCAAACCCCCTTGGGGGCaa                                                           >  1:378474/1‑149 (MQ=2)                                                                                               aCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACg                                                         <  2:279757/151‑1 (MQ=255)                                                                                                 tttCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTT‑GAACCCCCCCTAAGTTGCCCGATAAAC‑A‑CCTTTCCGGCGGGCCCCTTCCGCCCCTCGGGCAACCCC‑CCCAAATGTTTTTCTTCCAAACCTCATGGGGGGGAACggg                                                       >  1:173255/1‑151 (MQ=9)                                                                                                 tttCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGTTT‑TACGCCCCCATCCGTTGGCTTAGACTC‑A‑CATTCCCGGCTTGGATCCTCAAAAACGCCGACACCGCA‑ACCAAATGTTTTTCTACCCACCCTcctaggg                                                                >  2:698822/1‑136 (MQ=2)                                                                                                  ttCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTT‑GGAACCCCCATACGTTTCCGGATACAC‑A‑CTTTTCAGGCGGGGTCCTTCAGCCCCCCCGGCACCCCACCCAAATTTTTTTG‑TACCAAACCCCATGGGGGGCAACcggg                                                      >  1:5126/1‑151 (MQ=9)                                                                                                  ttCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg                                                       <  2:198282/150‑1 (MQ=255)                                                                                                     gTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑GCACCCCCCATAAGGTGTCGGATACAC‑A‑CTTTCCCGGCGTGCGCCCTCAGCCACTCGGACACCCCA‑CCCAAATGTTTTTTTTCCCAAACTCATGGGGGGGAAAGGGgcg                                                    >  1:437747/1‑150 (MQ=2)                                                                                                     gTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑GAACCCCCCATACGTTTCCCTATACAC‑A‑CTTTCCAGGCGGGCCCCTTCACCCCCCCGGACCCCCCC‑CCAAATTTTTTTGTTACCCAACCCCATGGGGGGGAACgggg                                                      >  1:395683/1‑148 (MQ=14)                                                                                                      tACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGGTC‑GAACCCCCCATAAGTTTTCGTATACAC‑A‑CCTTCCAGGCGGGCTCCTTCAGCCCCACGGACACCCCC‑CCAAAATGTTTTGTTATCAAACCCCCTGGGTGGGAACGGGgcg                                                    >  2:603794/1‑149 (MQ=14)                                                                                                      tACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑GAAACCCCCATACGTTGCCGTATAAAC‑A‑CTTTTCAGGCGTGCTCCCTCAGCCACTCCGACACCCCA‑CCAAAATTTTTTTCTACCAAAACTCATGGGGGGCAAAgggggg                                                    >  1:625652/1‑147 (MQ=14)                                                                                                            ctctTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GACCCCCCGATACGTTGCCATATACTC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCTCTCGGACACCTCG‑CCAATGTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGGGCTTCtat                                             <  2:166148/150‑1 (MQ=17)                                                                                                              cttcttTAAATATGGCGGTGAGGGGGGGGTTT‑GAACCCCCGATACGTTGCCGGATACAC‑A‑CTTTTCAGGCGGGCTCCTTCAGCCACCCGGACACCTCA‑CCCAAATGTTTTTCTACCAAACCTCATGGGGGGCAACGGGGGGCTACTATAgg                                          >  1:591656/1‑151 (MQ=18)                                                                                                               ttcttTAAATATGGCGGTGAGGGGGGGGGTC‑GAACCCCCCATAAGTTTACGTATAAAC‑A‑ATTTTCCGGGGGGGTCCTTTAGCCACCCGGCCACCCCA‑CCAAATTTTTTTTTTACCCAACCTCATGGGGGGCGACAGGGGGCCACTATAggg                                         >  2:393617/1‑151 (MQ=9)                                                                                                                   ttAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACTCCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAg                                       <  1:952138/149‑1 (MQ=255)                                                                                                                     aaaTATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTg                                    <  1:94074/150‑1 (MQ=255)                                                                                                                      aaTATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGa                                  <  1:972066/151‑1 (MQ=255)                                                                                                                           ggCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTaa                              <  1:242051/150‑1 (MQ=255)                                                                                                                             cGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAAc                           >  1:1135580/1‑151 (MQ=255)                                                                                                                             cGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAAc                           >  2:502722/1‑151 (MQ=255)                                                                                                                              ggTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACg                          >  1:465517/1‑151 (MQ=255)                                                                                                                              ggTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACg                          <  2:693207/151‑1 (MQ=255)                                                                                                                                 gAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTc                       >  2:1003974/1‑151 (MQ=255)                                                                                                                                 gAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTc                       <  2:1055904/151‑1 (MQ=255)                                                                                                                                  aGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGCTGGAGTAAAACGGTc                       >  1:1028517/1‑150 (MQ=255)                                                                                                                                    ggggggATTC‑GAACCCCCGATACGTTGCCGTATACAC‑G‑CTTTCCAGACGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTAGCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaag                    <  2:762858/151‑1 (MQ=255)                                                                                                                                     gggggATTC‑GAACCCCCGATTCGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaaga                   <  1:196039/151‑1 (MQ=255)                                                                                                                                        ggATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaaga                   >  1:591746/1‑148 (MQ=255)                                                                                                                                          aTTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAAt              <  1:420797/151‑1 (MQ=255)                                                                                                                                             c‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAAtttt           <  1:1014258/151‑1 (MQ=255)                                                                                                                                                    cccGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGAt     >  1:651416/1‑151 (MQ=255)                                                                                                                                                    cccGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGAt     >  1:713738/1‑151 (MQ=255)                                                                                                                                                       gATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAat  >  2:435440/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                          GTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑GAACCCCCGATACGTTGCCGTATACAC‑A‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAAT  >  NC_000913/925763‑926062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑