Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,646	0	T	G	47.1%	107.7 / 16.4	72	V316G (GTA→GGA)	yeiI	putative kinase
Reads supporting (aligned to +/- strand):  ref base T (5/32);  new base G (33/0);  total (40/32)
Fisher's exact test for biased strand distribution p-value = 5.23e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATG  >  NC_000913/2252496‑2252793                                                                                                                                                        |                                                                                                                                                          atCAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGt                                                                                                                                                           <  2:774026/151‑1 (MQ=255)     gCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGtatat                                                                                                                                                       <  1:1060162/151‑1 (MQ=255)        aGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGgat                                                                                                                                                         >  2:833836/1‑146 (MQ=255)             ttCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGATATA‑Gtttttt                                                                                                                                              >  1:97193/1‑149 (MQ=255)             ttCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCCGGgattttatttttt                                                                                                                                               >  2:664636/1‑141 (MQ=255)               cAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGGCCGGGGTATA‑GAtttttt                                                                                                                                             >  2:109491/1‑150 (MQ=255)               cAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGATATA‑GCtttttt                                                                                                                                             >  1:310443/1‑150 (MQ=255)                 gCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGATATA‑GTtttttt                                                                                                                                             >  2:233806/1‑148 (MQ=255)                   aaCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAgg                                                                                                                                        <  2:822234/151‑1 (MQ=255)                     cTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GTtttttt                                                                                                                                             >  1:826210/1‑144 (MQ=255)                       gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGAt                                                                                                                                      <  1:880249/149‑1 (MQ=255)                       gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATa                                                                                                                                     <  1:925697/150‑1 (MQ=255)                       gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCCGGGAATTA‑GTTTTCttttcag                                                                                                                                        >  2:595859/1‑142 (MQ=255)                        tttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCTGG‑GTATA‑GCttttttttatgaga                                                                                                                                     >  2:793676/1‑141 (MQ=255)                         ttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTGTATA‑ACtttttt                                                                                                                                             >  1:453105/1‑140 (MQ=255)                         ttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACa                                                                                                                                   <  1:471421/150‑1 (MQ=255)                          tgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGGCGGGGATAAA‑GCTTTTTTGTAGGaaaa                                                                                                                                    >  1:814600/1‑145 (MQ=255)                           gtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGGCTGGGATATA‑ACTTTTTTTTAGGATAAAGtt                                                                                                                                >  1:222587/1‑151 (MQ=255)                           gtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGtt                                                                                                                                <  2:387947/151‑1 (MQ=255)                           gtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGt                                                                                                                                 <  1:135469/150‑1 (MQ=255)                           gtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGt                                                                                                                                 <  2:362124/150‑1 (MQ=255)                             ttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGttt                                                                                                                               <  2:520712/150‑1 (MQ=255)                             ttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGGTATA‑GCTTTTTTTTAGGAGACAGttt                                                                                                                               >  1:769599/1‑150 (MQ=255)                             ttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCCGGGATATA‑GCttttttttagggtaaaggtt                                                                                                                               >  1:236406/1‑136 (MQ=255)                              tATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTc                                                                                                                              <  2:229431/150‑1 (MQ=255)                               aTTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTcc                                                                                                                             <  2:1086754/150‑1 (MQ=255)                               aTTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCCGGTATATA‑ACTTTCTTTTAGGAGAAAGttt                                                                                                                               >  1:886326/1‑148 (MQ=255)                                tttGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGATATA‑GCTTTTTTTTAGGGTACAGttt                                                                                                                               >  1:498344/1‑147 (MQ=255)                                 ttGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCcgc                                                                                                                           <  1:319014/150‑1 (MQ=255)                                       gATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGATATA‑GCTTTTTTGTAGGGTAAAGGTTTCGCGGCa                                                                                                                       >  1:550475/1‑148 (MQ=255)                                           aGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCCGGGATTTA‑TCTTTTTTTTTGGATACAGgttttccgcccgcgccc                                                                                                                 >  2:392139/1‑136 (MQ=255)                                            gTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGt                                                                                                              <  2:879586/151‑1 (MQ=255)                                              cAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGtt                                                                                                             <  2:574484/150‑1 (MQ=255)                                               aGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGCTTTATGGC‑GGGCCTGGGATATA‑ACTTTTTTGAAGGGTACAGGTTTCCCGACAAC‑GCCCGtttt                                                                                                           >  2:292866/1‑151 (MQ=255)                                                          gCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGATATA‑ATTTTTTTTATGGAGATAATTTTTCCGACAGC‑GCCCGGTTTGCgggggcc                                                                                                  >  1:53129/1‑144 (MQ=255)                                                               aaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGATATA‑AATTTCTTGTAGGATACAGTTTTCCCGGCAGC‑GCCCGCTTTTTGGGAGCCTGCGCgg                                                                                           >  1:1094946/1‑151 (MQ=255)                                                                  gATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCGGGGATTTA‑TCTTTCTTTTAAGGTACAGTTTTCTCGGCAGC‑GCCCCGCTTTCGGTAGCCTGCGCgg                                                                                           >  2:463332/1‑148 (MQ=255)                                                                   aTGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AAt                                                                                      <  1:714964/151‑1 (MQ=255)                                                                    tGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCCGGGGTATT‑GCTTTCTTTTAGGGTAAAATTTTCGCCGCAGC‑GCCCCTTTTTCCGTAGCCTGCCCgggcaat                                                                                      >  2:803065/1‑146 (MQ=255)                                                                            aaTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGATATA‑GCTTTTTTTAAGGATACAGTTTTCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AAttt                                                                                    >  1:919878/1‑144 (MQ=255)                                                                            aaTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGATATA‑GCTTTTTTTAAGGATACAGTTTTCGCGACAGC‑GCCCGGTTTTCGGGAGCCTGCGCGGG‑CATTTtgcgc                                                                               >  1:1096054/1‑149 (MQ=255)                                                                             aTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCCGGTGTATA‑TGTTTTTTTTAGGAGAAAATTTTCCCGGCACC‑GCCCCCTTTTTGGGAGCCTGCGCCGCGAAttttcgc                                                                                >  2:1084521/1‑144 (MQ=255)                                                                             aTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCCGGGATATA‑GATTTCTTTTAGGATACAGTTTCCGCGCCAGC‑GCCCGTTTTTTGGGAGCCCGCGCGGG‑CATTTCGCGCGcc                                                                            >  1:16782/1‑151 (MQ=255)                                                                               ttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCa                                                                           <  2:937853/150‑1 (MQ=255)                                                                               ttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCa                                                                           <  1:471864/150‑1 (MQ=255)                                                                                tttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGTTATA‑GCTTTTTTTTAGGGTACAGTTTTCGCGGCCGC‑GCCCCCTTTTCGGGTGCCTGCGCGGC‑GATTTttcgcg                                                                              >  2:140883/1‑146 (MQ=255)                                                                                   gCTGACCGTGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑gg                                                                      <  2:126674/150‑1 (MQ=255)                                                                                       aCCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGATATA‑GCTTTTTTTTAGGATACAGTTTCCGCCGCAGC‑GCCCGCTTTTCGGGAGCCTGCGCGGC‑AATTTTGCGCGCCCGC‑gg                                                                      >  1:435494/1‑146 (MQ=255)                                                                                             ccAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCGGGGGATATA‑GCTTTTTTTTAGGGTAAAGATTCCCCGACACA‑GCCCGTTTTTGGGGAGCCTGCGCGGG‑GAATTTGCGCGCCAGC‑GGCCG‑cctcaa                                                            >  2:926938/1‑144 (MQ=255)                                                                                             ccAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGCCCCGCCATATA‑ACTTTTTTTATGGGGACAGATTCCCCGCCAGC‑GCCCCGTTTTCGGGATACCCCGCGGC‑AATTTCACGCGCCCGC‑gggcgcct                                                                >  1:103311/1‑140 (MQ=255)                                                                                              cAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGGCCGGGGTATA‑TCATTCTTTGAGGGTACAGTTTTCGCGGCAGC‑GCCCCCTTTGCGGTAGCCCCCGCGGC‑CATTTCGCGCGCCAGC‑GGCCGCC‑TTaaaa                                                          >  1:414364/1‑149 (MQ=255)                                                                                                gcgcATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAacc                                                      <  2:258556/151‑1 (MQ=255)                                                                                                       cGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGGCCGGGATATA‑GCTTTTTTTTAGGATACAGTTTTCGCGGCAGC‑CCCCCCTTTTCGGTAGCCTGCGCGGC‑CATTTTTCGCGCCCGC‑GGCCC‑CGTTAAAAAaccccccc                                                 >  2:1126320/1‑146 (MQ=255)                                                                                                       cGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAaccctaccct                                               <  2:675592/151‑1 (MQ=255)                                                                                                       cGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCCGGGATATA‑GCTTTTTTTTAGGGTAAAGTTTTTGCGACAGC‑GCCCGCTTTTCGGGAGGCTGCGGGGC‑GATTTTGCGCGCCCGC‑GGGCG‑C‑CTTAACAacccccccc                                                >  2:769119/1‑145 (MQ=255)                                                                                                       cGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCCCGGATATA‑TGTTTTTTGTAGGATACAGATTTCGCGGCAGC‑CCCCCCTTTTCGGTAGGCTGCGCGGC‑AAATTCCCCCGCCACC‑GGCGG‑C‑CTTAACAaacccccc                                                 >  2:790745/1‑145 (MQ=255)                                                                                                        gACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGGCTGGGGCATA‑TATTTTTTTTAGGGGACAATTTTCCCGGCAGCGGCCCGTTTT‑CGGTAGGCTTCGCGGC‑AACTTTTCCCGCCCGC‑GGCAG‑C‑GTTAACAaacccaccc                                                >  2:744233/1‑149 (MQ=255)                                                                                                        gACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTg                                              <  2:769483/151‑1 (MQ=255)                                                                                                         aCAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGt                                             <  1:797566/151‑1 (MQ=255)                                                                                                                aCAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGa                                      <  1:412149/151‑1 (MQ=255)                                                                                                                 cAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGAt                                     <  1:1082113/151‑1 (MQ=255)                                                                                                                  aGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGATATA‑GCTTTTTTTTAGGGAAAAGTTTTCGCGACAGC‑GCCCGTTTTTCGGTAGCCTGCGCGGC‑AAATTCTCGCGCCCGC‑GGCAG‑C‑TTAAAAAACCCCACCCCGTCTGcc                                        >  1:452949/1‑147 (MQ=255)                                                                                                                    ttttGGTGCTGACGATGGTTTTATGGC‑GGGCCCGGGATATT‑TTTTTCCTTTAGGGGACAGTTTTCGCGACAGC‑GCCCCCTTTTTGGGAGGCTGCGCGGG‑AATTTCTCGCGCCAGC‑GGCAG‑C‑TTTAAAAACCCTACCCCCTCTTCCGATaa                                   >  1:978629/1‑150 (MQ=255)                                                                                                                      ttGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAAcg                                 <  1:572808/150‑1 (MQ=255)                                                                                                                      ttGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAAcg                                 <  1:440179/150‑1 (MQ=255)                                                                                                                      ttGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGGGTATA‑GGTTTTTTTAAGGGAAAAATTTTCGCGCCAAC‑GCCCCTTTTTCCGGGGGCCGCGCGGC‑ACATTTTCGCGCCCGCGGGCGG‑C‑TTAAAAAacccaccccttcttccgcaaa                                    >  1:597118/1‑137 (MQ=255)                                                                                                                        ggTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACgcgc                              <  1:495448/151‑1 (MQ=255)                                                                                                                          tGCTGACGATGGTTTTATGGC‑GGGCCTGGGGTTTATTCTTTTTTTAA‑GAGAAAATTTTCCCGCCAAC‑GGCCGGTTTGCTGTAGTCTGCGCGGG‑AATTTTTCGCGCCACC‑GGCCG‑C‑TTAAACAacccctcccttcttttc                                        >  1:73767/1‑132 (MQ=255)                                                                                                                             tGACGATGGTTTTATGGC‑GGGCCCGGGATATA‑GCTTTTTTTTAGGAGAAAGTTTTCGCGGCAGC‑GCCCGCTTTTCGGGGGCCTGCGCGGC‑AATTTCGCGCGCCCGC‑GGCAG‑C‑TTAAAAAAACCCACCCCGTCCGCCGCTaaacc                                >  2:382832/1‑141 (MQ=255)                                                                                                                                            ggC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgt           <  1:267496/150‑1 (MQ=255)                                                                                                                                              c‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTa        <  1:138989/151‑1 (MQ=255)                                                                                                                                                gggCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTa        >  2:703559/1‑150 (MQ=255)                                                                                                                                                  gCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACa     <  2:498256/151‑1 (MQ=255)                                                                                                                                                      ggTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATg  <  2:742689/150‑1 (MQ=255)                                                                                                                                                      ggTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATg  <  2:1046266/150‑1 (MQ=255)                                                                                                                                                      ggTATATA‑GCTCTCTTGGAGGATACACTTGCCGCGACCGG‑TCCCGTTATGCGGTAGCCTGCGCGTG‑CATTGCGCGCGCCACC‑GGTAG‑C‑TTAAACATTCCTACCCTGTCTTCGGATAACGCGCTTTCGTAAGTGCCAATGGTGTAACAAt   <  2:876988/149‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                          ATCAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGC‑GGGCCTGGTATATA‑GCTTTCTTGAAGGATACAGTTTCCGCGACAGC‑GCCCGTTTTGCGGTAGCCTGCGCGGC‑AATTTCGCGCGCCAGC‑GGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATG  >  NC_000913/2252496‑2252793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑