Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,511,772	0	A	C	43.1%	130.5 / 22.0	72	V312G (GTG→GGG)	mntH	manganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base A (39/2);  new base C (0/31);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 2.37e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACACCAGTATGACCGGAAAAGT  >  NC_000913/2511623‑2511922                                                                                                                                                      |                                                                                                                                                       gagCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATcaccac                                                                                                                                                       >  2:143102/1‑151 (MQ=255) gagCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATcaccac                                                                                                                                                       <  1:323088/151‑1 (MQ=255)   gCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCt                                                                                                                                                     >  2:469647/1‑151 (MQ=255)         ccAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGcccc                                                                                                                                                >  1:1020509/1‑150 (MQ=255)          cAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGc                                                                                                                                              >  2:426854/1‑151 (MQ=255)           aaaaCTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGcc                                                                                                                                             >  1:843513/1‑151 (MQ=255)             aaCTTAACAGCACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGcc                                                                                                                                             >  1:339365/1‑149 (MQ=255)               cTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAgtg                                                                                                                                         >  1:596093/1‑151 (MQ=255)               cTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAgtg                                                                                                                                         >  2:494613/1‑151 (MQ=255)                ttAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAgtgt                                                                                                                                        >  1:527251/1‑151 (MQ=255)                 tAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTc                                                                                                                                       >  2:344750/1‑151 (MQ=255)                  aaCAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTcc                                                                                                                                      >  2:1135240/1‑151 (MQ=255)                     aGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTcccc                                                                                                                                    >  2:514968/1‑150 (MQ=255)                       tACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAACGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCccacc                                                                                                                                 >  1:8248/1‑151 (MQ=255)                              cTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTg                                                                                                                          >  2:328224/1‑151 (MQ=255)                                cacaccaagatgccGGGTCGGATCTAACCCCGTCAAAATGAAAATAAATGTCGCCGAAATGTGGCGTGAACGACGCCCCCGCAGCGGAATTGGAAGGCAAGTGATTCTTTGTACCGCCACCTGCCCCGCCAGTGTCCCCACCACCGTTGa                                                                                                                         <  2:166496/138‑1 (MQ=255)                                 aaaacaaaaacccGTGTGGAATCTAATCCCATCAAAATGAAAAAAAATGACGCAAAAATGGTGATTGTACGACGCCCCCCAGGGGGGATATGAAAGCGAATGAGCCCCTGCATCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTg                                                                                                                          <  1:1114148/137‑1 (MQ=255)                                     ccAAAATCCGGGTCGGTTCTAACCCCAAAAAAAATACAAAAAACGACGGCAACATGGGGACGTTCCGCCGCCCCCACAGCGGGAAATGAAAGCGAAGGACGCCCTGCTCCCCCCCTTGCCCCGCCAGTGTCCCCACCACCGTTGAAGAc                                                                                                                     <  2:407511/149‑1 (MQ=255)                                       aGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGcc                                                                                                                 >  1:278195/1‑151 (MQ=255)                                       aGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGcc                                                                                                                 >  2:45409/1‑151 (MQ=255)                                         aacccGGGTCGGTCCTAACCCCACCAAAATGAAAATAAAGGACGGCAACAGGGTGACTGTCCGCCCCCCCCCCAGCGGGATAGGAAAGCGAATGAAGCCCTGCACCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGcccc                                                                                                               <  2:1074445/148‑1 (MQ=255)                                                   ggATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAAcc                                                                                                      >  1:484486/1‑150 (MQ=255)                                                   ggATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAAcc                                                                                                      >  2:88666/1‑150 (MQ=255)                                                   ggATCTAACCCCAACAGAATAACAAAAAATGACGGAAACATGTTGCTTGTACGACCCCCCCCCGGCGGGATATAAAACCGAATGAAGCCCTGCACCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCgca                                                                                                            <  2:427710/144‑1 (MQ=255)                                                   gaaTCAAACCCCATCAAAAGGAAAATAAATGACGGCAACAGGGTGACTGTCCGACGCACCCACAGCGGAATATGAAGGCGAATGAAGCCCTGCATCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCaa                                                                                                        <  1:803052/146‑1 (MQ=255)                                                       cTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTTCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCt                                                                                                 >  1:178449/1‑151 (MQ=255)                                                        tAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCtt                                                                                                >  2:525427/1‑151 (MQ=255)                                                         aaTCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCtt                                                                                                >  2:514377/1‑150 (MQ=255)                                                           tCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAAt                                                                                             >  1:764546/1‑151 (MQ=255)                                                             ccAGCAGAATGAAAAAAAATGACGGCAACATGGTGACTGTCCGCCCCCCCCACAGCGGGATATAAAAGCAAATGAAGCCCTGCACCACCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATcc                                                                                           <  1:932842/151‑1 (MQ=255)                                                                    aaggaaaaTAAAGGCCGGCAACAGGGTGCCTGTACGACCCCCCCACAGCGGGATATGAAAGCAAATGAACCCCTCCATCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGAcc                                                                                    <  1:505734/145‑1 (MQ=255)                                                                    aaTGAAAAAAAATACCGCCAACATGGTGACTGTACGACCCCCCCACGGGGGGAAAGGAAACCGAAGGAACCCCTCCACCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCaaa                                                                                        <  2:789830/147‑1 (MQ=255)                                                                           aTAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCg                                                                             >  1:686456/1‑151 (MQ=255)                                                                            tAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGcc                                                                              >  1:632433/1‑149 (MQ=255)                                                                            tAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGc                                                                            >  2:177766/1‑151 (MQ=255)                                                                            tAAATGACGGCAACATGGTGACTGTACGACGCACACACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGc                                                                            >  1:877827/1‑151 (MQ=255)                                                                              aaTGACGCCAACATGGTGACGGTCCGACGCCCCCACAGCGGGATATAAAAGCAAATGAAGCCCTGCACCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAg                                                                          <  1:191626/151‑1 (MQ=255)                                                                               atgcgggCAACGTGGTGCCTGTCCGACGCCCCCACAGCGGGATATAAAAGCAAATGAAGCCCTGCACCACCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGc                                                                         <  1:818229/146‑1 (MQ=255)                                                                                tGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCg                                                                        >  1:1128718/1‑151 (MQ=255)                                                                                ggccGGCAACATGGTGACGGTACGCCCCCCCCACAGGGGGATAGGAAGGCAAATGAAGCCCGGCATCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGc                                                                         <  2:807603/147‑1 (MQ=255)                                                                                    ggaaaCATGGGGCCTGTCCGACGCCCCCACGGGGGGATATAAAGGCGAAAGAAGCCCTCCACCCCCCCCGCCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTg                                                                      <  2:760957/146‑1 (MQ=255)                                                                                    ggCAACATGGTGCCTGTACGCCGCCCCCACAGGGGGATATGAAAGCGAATGAAGCCCTGCATCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCg                                                                        <  1:108082/147‑1 (MQ=255)                                                                                       aaCATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCtt                                                                  >  2:877587/1‑150 (MQ=255)                                                                                        aCATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTa                                                                 >  2:218085/1‑150 (MQ=255)                                                                                            ggTGACTGTCCGCCGCCCCCACGGCGGAATAAGAAAGCGAATGAACCCCGGCATCCCCCCCGGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACa                                                              <  2:274730/149‑1 (MQ=255)                                                                                            ggTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGc                                                            >  1:518971/1‑151 (MQ=255)                                                                                            ggTGACTGCACGACCCCCCCACGGCGGAATATAAAACCGAATAAAGCCCTGCACCCCCCCCCCCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACa                                                              <  2:756096/149‑1 (MQ=255)                                                                                             gTGACTGTACGCCGCCCCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCACCCCCCCCGGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCg                                                           <  2:401906/151‑1 (MQ=255)                                                                                             gTGACTGTACGACGCCCCCACAGGGGGATAGGAAAGCGAATGAGCCCCTCCACCACCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCg                                                           <  2:1006377/151‑1 (MQ=255)                                                                                                     acgacgCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGc                                                   >  1:351291/1‑151 (MQ=255)                                                                                                         cgCCCCCACAGCGGGATATGAAAGCGAATGAACCCCTGCATCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTc                                                <  1:514466/150‑1 (MQ=255)                                                                                                           cACCCCCGGGGGGAGAGGAAAGCCATTAAACCCCTGCACCCCCCCCCCCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCa                                                     <  2:298538/143‑1 (MQ=255)                                                                                                           cACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTc                                                >  2:891471/1‑148 (MQ=255)                                                                                                              ccACGGCGGGATATGAAAGCGAATGACGCCCTGCATCCCCCCCTCCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATaa                                          <  2:1116506/151‑1 (MQ=255)                                                                                                               cacaGCGGGATATGAAAGCAAAGGAAGCCCTGCACCCCCCCCTCCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAg                                         <  1:435554/151‑1 (MQ=255)                                                                                                                     gggttagaaaaGAGAAGGAGGCCCGACACCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCtca                                    <  2:643481/142‑1 (MQ=255)                                                                                                                     gggATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCtcat                                   >  1:921192/1‑151 (MQ=255)                                                                                                                      ggATATGAAAGCGAATGAACCCCGGCACCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCtcatc                                  <  1:393173/151‑1 (MQ=255)                                                                                                                         tatGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCCCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGGGCCTTAACAGCGGTTGCAGCGTCAGATAAGCCtcatca                                 >  2:467202/1‑149 (MQ=255)                                                                                                                           tGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCtcatca                                 >  2:174594/1‑147 (MQ=255)                                                                                                                             aaaggaaaTGAAGCCCGCCACCCCCCCCTCCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGAt                             <  2:1042048/143‑1 (MQ=255)                                                                                                                             aaaGCGAATGAGCCCCTGCATCCCCCCCTCCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATc                            <  1:499646/150‑1 (MQ=255)                                                                                                                             aaaGCGAATAAGCCCCTCCACCCCCCCCGCCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATc                            <  2:1037953/150‑1 (MQ=255)                                                                                                                             aaaGCGAAAGAAGCCCTGCAACACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCtcatca                                 <  1:133649/145‑1 (MQ=255)                                                                                                                              aaGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCtcatca                                 >  1:659375/1‑144 (MQ=255)                                                                                                                              aaGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGCGGTTTAACAGCGGTTGCAGCGTCAGATAAGCCtcatc                                  >  1:458088/1‑143 (MQ=255)                                                                                                                                gagAATAAACCCCTGCATCCCCCCCCGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCgg                          <  1:714485/147‑1 (MQ=255)                                                                                                                                   aaTGAAGCCCTGCATCCCCCCCTCCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAaca                     <  1:489465/151‑1 (MQ=255)                                                                                                                                   aaTGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAac                      >  1:851482/1‑150 (MQ=255)                                                                                                                                     tGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACAcc                   >  2:788775/1‑151 (MQ=255)                                                                                                                                     ggaggCCCTGCACCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACAcc                   <  2:421098/147‑1 (MQ=255)                                                                                                                                       acgCCCTGCACCCCCCCCGGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACACCa                  <  2:871521/148‑1 (MQ=255)                                                                                                                                          cccTGCACCACCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACACCAGTAt              <  2:311424/151‑1 (MQ=255)                                                                                                                                             tGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACACCAGTATGAc           >  2:998328/1‑151 (MQ=255)                                                                                                                                                  caccacCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACACCAGTATGACCGGa       >  2:223076/1‑150 (MQ=255)                                                                                                                                                   accaccTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACACCAGTATGACCGGaa      >  2:558114/1‑150 (MQ=255)                                                                                                                                                    ccaccTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACACCAGTATGACCGGaaa     >  1:902487/1‑150 (MQ=255)                                                                                                                                                      accTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACACCAGTATGACCGGAAAAGt  >  2:810815/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                       GAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCGGTTGCAGCGTCAGATAAGCCTCATCAAGATCGGCAACACCAGTATGACCGGAAAAGT  >  NC_000913/2511623‑2511922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑