Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,578,143	0	A	C	44.4%	94.2 / 26.4	63	L79V (TTA→GTA)	maeB	malic enzyme: putative oxidoreductase/phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base A (27/8);  new base C (0/28);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 5.21e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCC  >  NC_000913/2577996‑2578291                                                                                                                                                      |                                                                                                                                                     gttCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAAccc                                                                                                                                                   <  2:85593/151‑1 (MQ=255)   tCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAAcccc                                                                                                                                                  <  1:409820/150‑1 (MQ=255)     gAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGc                                                                                                                                               >  2:637438/1‑151 (MQ=255)      aGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGc                                                                                                                                               <  2:930293/150‑1 (MQ=255)       gcgcGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCAc                                                                                                                                             <  1:1000244/151‑1 (MQ=255)       gcgcGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCAc                                                                                                                                             <  2:660003/151‑1 (MQ=255)        cgcgGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCAcc                                                                                                                                            >  2:1116100/1‑151 (MQ=255)        cgcgGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCAcc                                                                                                                                            <  1:816109/151‑1 (MQ=255)         gcggcgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCAccg                                                                                                                                           <  2:558204/151‑1 (MQ=255)              gACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGt                                                                                                                                       >  2:827678/1‑150 (MQ=255)                    ttCAATAATTTTGTCCGGGTCGAGTTCGTCACCTTCAATGTCAAATACATCAACCCCGGCGATTTTCTTAAACAGACCGCCCTTGCCTCCCATCCCCGG‑TTT‑GCCTGCCAGCGCGCAAATGTTGCCTACCCCCAGCACCGCCGTACCGt                                                                                                                                  <  2:83512/149‑1 (MQ=255)                      cAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGtt                                                                                                                                 >  1:264194/1‑148 (MQ=255)                             tttGTCCGGGTCGATTTCGTCACCTTAAATGTAAAATCCATCAACCCCGGCAAATTTCTTAAAAAGACCGCCCTTCCCTCCCACCCCCGT‑TTT‑GCCTCCCGGCGCGCCAATGTTCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCAc                                                                                                                        <  2:124819/150‑1 (MQ=255)                                  ccGGGTCGAGTTCGTCACCTTCAATGTAAAATACACCAACCCCGGCGATTTTCTAAAACAGACCCCCCTTCCCTCCCACCCCCGG‑TTT‑GCCTGCCAGCGCCCCATTGTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGcca                                                                                                                   <  2:230206/150‑1 (MQ=255)                                    ggttCGAGTTCGTAAATTTAAATGAAAAATACACCAACCCCGG‑GAATTTTCTAAAAAAAACCCCCTTTCCCTCCCACCCCGGTTTT‑CCCGGCCGGCGCCCAATTGTTCCCAACCCCCAGCACCGCCGTACCGTTAGAGATCACCGccacc                                                                                                                 <  1:592877/147‑1 (MQ=255)                                       tCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGt                                                                                                             >  2:934405/1‑151 (MQ=255)                                            ttCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCt                                                                                                        >  1:746810/1‑151 (MQ=255)                                            ttCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCt                                                                                                        >  2:68708/1‑151 (MQ=255)                                              cGTCATCTTCATTGTCAAAAACATCAACCCGGGAGTATTTCTAAAGAAAAACGCCTTTG‑CTTCCCCCCCCGG‑TTTGGCCTGCAGGCGCGCAATTTTTCCCTCCCCCCGCCTCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTc                                                                                                       <  2:831508/150‑1 (MQ=255)                                               gTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCgg                                                                                                     >  1:473047/1‑151 (MQ=255)                                                  aatttCAATGTAAAATACACCAACCCCGGAGAATTTCTAAAACAGAACCCCTTTGCCTCCCACCCCCGG‑TTT‑GCCTGCCAGCGCGCAAATGTGGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCggg                                                                                                    <  1:854697/146‑1 (MQ=255)                                                  aaCTTAAATGTCAAATCCATCACCCCCGGCGAATTTCTAAAACAGACCGCCTTTCCCTCCCACCCCCGT‑TTT‑GCCGCCCGGCGCCCAATTGTTGCCTACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCg                                                                                                      <  1:421900/147‑1 (MQ=255)                                                  aaCTTAAATGTAAAATACATAAACCCCGGCGATTTTCTAAAACAAACCCCCCTTCCCTCCCACCCCCGT‑TTT‑CCCTGCCAGCGCGCAAATGTTCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCgg                                                                                                     <  1:458194/148‑1 (MQ=255)                                                       cAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGtat                                                                                              >  2:187788/1‑150 (MQ=255)                                                        aaTGTCAAAAACACAAACCCCGGCAATTTTCTAAAACAGACCCCCCTTGCCTCCCACCCCGGT‑TTT‑GCCGCCCGGCGCCCCAATGTTGCCAACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGtat                                                                                              <  2:382038/149‑1 (MQ=255)                                                        aaTGTAAAATCCACCAACCCCGGCAATTTTCTAAAACAAACCGCCCTTCCCTCCCACCCCCGT‑TTT‑GCCTGCCAGCCCGCCAATGTTCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGta                                                                                               <  2:153431/148‑1 (MQ=255)                                                          tGTAAAATACACCAACCCCGGCAAATTTCTAAAACAGACCGCCCTTGCCTTCCACCCCCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATAtt                                                                                           <  1:1121446/150‑1 (MQ=255)                                                           gtaaaaTACATCACCCCGGGCGATTTTCTAAAACAGACCCCTCTTCCCTCCCACCCCCGG‑TTT‑GCCTGCCAGCGCGCAAATTTTGCTAACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATAtt                                                                                           <  2:1090534/146‑1 (MQ=255)                                                           gtaaaaTACACCAACCCCGGCAATTTTTTAAAACAGAGCCCCCTGGCCTCCCCCCCCGGT‑TTT‑GCCCGCCGGCGCCCAAATGTGGCCAACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg                                                                                         <  1:754260/148‑1 (MQ=255)                                                              aaaTACATCAACCCCGGCAATTTTCTTAAACAGAACCCCCTTCCCTTCCACCCCCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCTTACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTa                                                                                       <  1:200819/150‑1 (MQ=255)                                                              aaaTACATCAACCCCGGCAAATTTCTAAAACAAAACGCCCTGGCCTCCCATCCCCGT‑TTT‑GCCTGCCAGCGCGCAAATGTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAg                                                                                      <  1:672855/151‑1 (MQ=255)                                                                  aCATAAACCCCGGCGATTTTCTTAAACAGACCCCCTTTCCCTCCCACCCCCGT‑TTT‑CCCTGCCAGCGCGCCAATTTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGc                                                                                    <  1:646067/149‑1 (MQ=255)                                                                       acccccGGCGAATTTCTAAAACAGACCGCCCTTCCCTCCCATCCCCGG‑TTT‑GCCTGCCAGCGCGCAAATGTTGCCTACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTaa                                                                              <  2:692210/147‑1 (MQ=255)                                                                       aaccccGGCGAATTTCTTAAACAGACCGCCCTGCCCTCCCATCACGGG‑TTT‑CCCGGCCGGCCCGCAAATGTTGCCAACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTaa                                                                              <  1:841512/147‑1 (MQ=255)                                                                       aaccccGGCAATTTTTTAAAACAGACCCCCCTTCCCTCCCACCCCCGT‑TTG‑GCCGGCCAGCGCGCCATTTTTGCCAACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTa                                                                               <  1:57002/146‑1 (MQ=255)                                                                       aaccccGGCAATTTTCTAAAACAGAACCCCTTTCCCTCCCACCCCGGT‑TTT‑GCCTGCCAGCGCCCAAATGTTCCCAACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAAc                                                                             <  1:1140364/148‑1 (MQ=255)                                                                        acccgggCGAATTTCTAAAACAGACCCCCCTTCCCTTCCACCCCGGG‑TTT‑GCCTCCGAGCGCGCAAATGTCGCCAACCCCCAGCACCGCCGTACCGTTAGAGATCGCCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTaa                                                                              <  2:511347/144‑1 (MQ=255)                                                                             ggCAAATTTCTTAAACAGACCCCCTTTGCCTCCCATCCCCGT‑TTT‑GCCTCCCAGCGCCCCAATGTTGCCAACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCt                                                                       <  1:674599/151‑1 (MQ=255)                                                                                gAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCtttt                                                                    >  2:92738/1‑151 (MQ=255)                                                                                  aTTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTc                                                                  >  1:644359/1‑151 (MQ=255)                                                                                   tttCTAAAACAGACCGCCTTTGCCTCCCACCCCCGG‑TTT‑GCCTGCCAGCGCGCAAATGTTGCCTACCCCCACCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTc                                                                  <  1:337541/150‑1 (MQ=255)                                                                                       ttAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAttt                                                             >  2:968271/1‑151 (MQ=255)                                                                                        tAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTc                                                            >  2:246020/1‑151 (MQ=255)                                                                                           aCAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCa                                                           >  1:232298/1‑149 (MQ=255)                                                                                             aGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGAc                                                       >  1:713327/1‑151 (MQ=255)                                                                                             aGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGAc                                                       >  1:65718/1‑151 (MQ=255)                                                                                                  gCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAgg                                                   >  2:317029/1‑150 (MQ=255)                                                                                                  gCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGt                                                  >  2:185801/1‑151 (MQ=255)                                                                                                   cccttcccttCCCCCCCCGT‑TTC‑GCCGGCCGCCGCGCCAATGTTCCCTACCCCCGCCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGt                                                  <  1:930215/150‑1 (MQ=255)                                                                                                       tGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGc                                             >  2:738522/1‑151 (MQ=255)                                                                                                         ccTCCCATCCCCGT‑TTT‑CCCTGCCAGCGCCCCAATGTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCa                                            <  1:1141547/150‑1 (MQ=255)                                                                                                         ccTCCCACCCCCGT‑TTG‑GCCGCCCAGCGCGCAATTTTTGCCAACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCg                                               <  1:777987/147‑1 (MQ=255)                                                                                                                      gtttt‑CCCTGCCACCGCGCAAATGTGGCCAACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAg                                <  1:637545/148‑1 (MQ=255)                                                                                                                      g‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGt                               >  1:107873/1‑150 (MQ=255)                                                                                                                        ttt‑GCCTGCCGGCGCGCAAATTTTGCCTAACCCCAGCGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTa                              <  1:375819/150‑1 (MQ=255)                                                                                                                         tt‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAgg                            >  1:307268/1‑151 (MQ=255)                                                                                                                              ctgccAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGcc                          >  2:27709/1‑149 (MQ=255)                                                                                                                                       cgcCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGcc                    >  2:1083606/1‑146 (MQ=255)                                                                                                                                        gcCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGcc                    >  1:228162/1‑145 (MQ=255)                                                                                                                                         ccAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcgc             >  2:1125317/1‑151 (MQ=255)                                                                                                                                           aaTGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCgcgc           >  1:1134714/1‑151 (MQ=255)                                                                                                                                           aaTGTTCCCAACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGAt                <  1:358053/146‑1 (MQ=255)                                                                                                                                             tGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCtg         >  2:171503/1‑151 (MQ=255)                                                                                                                                                      aCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGcc  >  1:480554/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                     GTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGG‑TTT‑GCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCC  >  NC_000913/2577996‑2578291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑