Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,001,1640AC46.2% 75.7 / 22.0 52T274P (ACC→CCC) xdhAxanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand):  ref base A (27/1);  new base C (0/24);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 5.86e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAA  >  NC_000913/3001020‑3001309
                                                                                                                                                         |                                                                                                                                                  
gTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGt                                                                                                                                               >  1:180058/1‑150 (MQ=255)
    gAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTAccc                                                                                                                                          >  1:1068277/1‑150 (MQ=255)
            aaaCCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTTCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCtt                                                                                                                                 >  1:63555/1‑151 (MQ=255)
                 aTTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTAcc                                                                                                                            >  1:378912/1‑151 (MQ=255)
                        ggTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTTCTGACCAGCAAGCTTGGCGGCATTCCGGGGAAAGTTTTCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGAc                                                                                                                      >  2:965656/1‑150 (MQ=255)
                        ggTGGCGGTTTTGAAAATAAAAGGATTGTGTGGGAGGACCAAATGCCGGCTTTCCGACCCAGCATTTTTGGCGGCTTCCGGGTAAAGTTTCCCCTGTCCGGTGAGGTTTTTTCCCCCGAACCCCGTCCCCGCCACGCTTTTACCATTGACg                                                                                                                     <  1:743004/151‑1 (MQ=255)
                          tGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTACCATTGACggg                                                                                                                   >  1:922023/1‑150 (MQ=255)
                             cGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCa                                                                                                                 >  1:813177/1‑150 (MQ=255)
                                ttttGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATg                                                                                                             >  2:718531/1‑151 (MQ=255)
                                 tttGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATgg                                                                                                            >  1:438722/1‑151 (MQ=255)
                                   tGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGc                                                                                                          >  1:1020040/1‑151 (MQ=255)
                                    ggTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCg                                                                                                         >  2:142443/1‑151 (MQ=255)
                                    gaaaaaaaaCGGGATGTACGGGAAGACCCAATGGCGGCTTTCCTGCCCAGCAAGTGGGGCGGCTTTCCGGTAAAATTTCCCCTTAGCCGTGAAGGGTTTTTCCCCCCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGc                                                                                                          <  1:144655/144‑1 (MQ=255)
                                      aaataaACAGGATGTACGGGAAGAGACAAGGGGGGCTTTCCGGCCAAGCAGGCTGGGGGGCTTCCGGGTAAATGTTCCCCTTAGCGGTGAAGTGTTTTCCCCCGCACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGc                                                                                                          <  1:179686/147‑1 (MQ=255)
                                       aaaaaaCGGGATGTACGGGAAGAGCAATTGGCGCTATCCCGGACCAGCAACGTTGGGGCATTGCCGGTAAAATTTCCCCTCACCCGGGAGGTTTTTTCCCCCGCACCCGGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCg                                                                                                         <  1:441290/145‑1 (MQ=255)
                                       aaaaaaCAGGATGTACGGGAAGAGCAAAGGGCGGCATTCCGGACCAGCAAGCTTGGCGGCTTTCGGGTAAAATTTCCCCTTAGCCGTAAAGAGTTTTCCCCCGCACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGa                                                                                                      <  1:1126533/148‑1 (MQ=255)
                                         taaACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAAc                                                                                                    >  2:489532/1‑151 (MQ=255)
                                            accaggtttttcTGGAAGAGCAAGGGGGGGCATTCCTGACCAGCAAGCTGGGCGGCATCCGGGAAAAATTTTCCTTGAGCCTGAAAGGGTTTTCCCTCGCACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACcg                                                                                                  <  1:1034121/140‑1 (MQ=255)
                                               ggATGTACGGGAAGACCCAAGGGCGGCTTTCCTGACCAGCAAGCTTGGCGGCATTCCGGTAAATTTTCCCCTTAGCCGTGAAGAGTTTTTCCTCGCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACcgc                                                                                                 <  1:187249/148‑1 (MQ=255)
                                                gtttGTACGGAAAGACCCAGTGGCGCCATCCCGACCCGGCAACTTGGGCGCCATCCGGGTAAATTTTCCCTGTGCCGGGAAAGAGTTTTCCCCCCCACCCCAACCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACcgc                                                                                                 <  2:1097899/145‑1 (MQ=255)
                                                     tACTGGAAGAGCAAAGGGCGGCTTTCCGGACCAGCAAGCTTGGCGGCATTCCGGTAAAATTTCCCCTTAGCCGTGAAGGGTTTTTCCCCGCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGaa                                                                                          <  1:884312/150‑1 (MQ=255)
                                                      aCTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAAc                                                                                         >  1:27894/1‑150 (MQ=255)
                                                        tGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACAtt                                                                                      >  1:249186/1‑151 (MQ=255)
                                                           aaGAGCAAAGGGCGGTATTCCTCACCAGCAAGTTTGGGGGCATTCGGGTGAAAGTTCCCCTTACCCGTAAAGAGTTTTCCCCCGCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGa                                                                                    <  1:871244/150‑1 (MQ=255)
                                                            aaggCCAATGGGCGCCATCCCTGACCAGCAGGTTGGGCGGCTTCCGGGTAAAATTTCCCCTTCGCCGTAAAGAGTGTTCCCCCCCACCCCTTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACa                                                                                        <  1:411798/143‑1 (MQ=255)
                                                                gccaggggcgccATCCCGGACCAGAAGGTTTGGCGCCTTTCCGGTAAAATTTCCCCTTAGCCGTGAAGAGTGTTTCCCCGCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGaaa                                                                                  <  2:579194/142‑1 (MQ=255)
                                                                   aagggcggcTTCCCTGACAAGAAAGTTTGGGGGCTTTCGGGTAAAATTTCCCTTTAGCGGTAAAGAGTTTTCCCCCGAACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTtat                                                                            <  1:779240/148‑1 (MQ=255)
                                                                    tGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTtat                                                                            >  1:583972/1‑149 (MQ=255)
                                                                            aTTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGAt                                                                   >  2:1017655/1‑151 (MQ=255)
                                                                                cTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTc                                                               >  2:526867/1‑151 (MQ=255)
                                                                                 tGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCt                                                              >  1:295689/1‑151 (MQ=255)
                                                                                   aCCAGCAGGCTTGGCGTCTTCCGGGTGAATTTTCCCTTTAGCCGGAAAGTGTTTTTCCCCGAACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCt                                                              <  2:1101868/149‑1 (MQ=255)
                                                                                      agaaaGCTGGGGGGCATCCCGGTGAATTTTCCCCTGAGCCGTGAAGAGTGTTTCCTCGCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCt                                                              <  2:929307/143‑1 (MQ=255)
                                                                                                 ggcggcctgcGGTGAAAAGTTCCCCTTTTCCGTGAAGTTTTTTCCCCGACACCCGTAACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGgc                                                 <  1:657803/141‑1 (MQ=255)
                                                                                                  ggcgcgattcgggtaaaTTTTCCCTTTCGCCGTGAAGGGTTTTTCCCCGCACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCgg                                                  <  2:912845/135‑1 (MQ=255)
                                                                                                    cATTCCGGTGAATTTTTCCCTTGGCCGTGAAGAGTGTTTCCCCGCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTAt                                           <  2:1107133/151‑1 (MQ=255)
                                                                                                            ggaaaatgTTCCCCTTAGCCGTGAGGATTTTTCCCCCGCACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGc                                         <  2:1130554/140‑1 (MQ=255)
                                                                                                              gaaaaaTTTCCCTTGCGCCGAGAAGGTTTTTCCCCCCCACCCCGACCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCa                                        <  2:502056/145‑1 (MQ=255)
                                                                                                                aaaGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACg                                 >  2:326499/1‑151 (MQ=255)
                                                                                                                 aaGTTTCCCCTTGGCCGGGAAGGGTGTTCCCCCCCACCCCGCACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCa                                   <  1:1072780/149‑1 (MQ=255)
                                                                                                                 aaGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACgg                                >  2:1035388/1‑151 (MQ=255)
                                                                                                                   gTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACggg                               >  2:586424/1‑150 (MQ=255)
                                                                                                                             tAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATc                      >  2:458626/1‑151 (MQ=255)
                                                                                                                                 ccGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCt                   >  1:198262/1‑151 (MQ=255)
                                                                                                                                 ccGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCt                   >  2:665491/1‑151 (MQ=255)
                                                                                                                                        aaggtttttcccccGCACCCCTTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTCCTg               <  1:538515/137‑1 (MQ=255)
                                                                                                                                             ttttcccgcGCACCCCTAACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGc              <  2:1015718/138‑1 (MQ=255)
                                                                                                                                                 tCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGa      >  2:708179/1‑150 (MQ=255)
                                                                                                                                                  ccccGCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCggggggg       <  1:748122/145‑1 (MQ=255)
                                                                                                                                                  ccccGCACCCAGTGCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCgggggg        <  2:267490/144‑1 (MQ=255)
                                                                                                                                                     cGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATaa  >  1:642439/1‑150 (MQ=255)
                                                                                                                                                      gCAACACGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATaa  >  2:389988/1‑149 (MQ=255)
                                                                                                                                                         |                                                                                                                                                  
GTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAA  >  NC_000913/3001020‑3001309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: