Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,001,164 | 0 | A | C | 46.2% | 75.7 / 22.0 | 52 | T274P (ACC→CCC) | xdhA | xanthine dehydrogenase, molybdenum binding subunit |
Reads supporting (aligned to +/- strand): ref base A (27/1); new base C (0/24); total (27/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.86e-14 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAA > NC_000913/3001020‑3001309 | gTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGt > 1:180058/1‑150 (MQ=255) gAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑Accc > 1:1068277/1‑150 (MQ=255) aaaCCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑TCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCtt > 1:63555/1‑151 (MQ=255) aTTTGTCGGTGGCGGTTTTGGTAATAAACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTAcc > 1:378912/1‑151 (MQ=255) ggTGGCGGTTTTGGTAATAAACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTTCTGACCAGCAAGCTTGGCGGCATT‑CCGGGG‑AAAGTTT‑TCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGAc > 2:965656/1‑150 (MQ=255) ggTGGCGGTTTTGAAAATAAA‑AGGATTGTGTGGGAGGACCAAAT‑GCCGGCTTTCCGACCCAGCATTTTTGGCGGC‑TT‑CCGGGT‑AAAGTTT‑CCC‑CT‑GTCCGGTG‑AGGTTTTTTCCCCCGAACCCCGT‑CCCCGCCACGCTTTTACCATTGACg < 1:743004/151‑1 (MQ=255) tGGCGGTTTTGGTAATAAACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGC‑TTTACCATTGACggg > 1:922023/1‑150 (MQ=255) cGGTTTTGGTAATAAACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCa > 1:813177/1‑150 (MQ=255) ttttGGTAATAAACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATg > 2:718531/1‑151 (MQ=255) tttGGTAATAAACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATgg > 1:438722/1‑151 (MQ=255) tGGTAATAAACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGc > 1:1020040/1‑151 (MQ=255) ggTAATAAACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCg > 2:142443/1‑151 (MQ=255) gaaaaaaaaCGGGA‑TGTACGGGAAGACCCAAT‑GGCGGCTTTCCTGCCCAGCAAGTGGGGCGGCTTT‑CCGGTA‑AAATTTC‑CCC‑TT‑AGCC‑GTG‑AAGGGTTTTTCCCCCCACCCCGT‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGc < 1:144655/144‑1 (MQ=255) aaataaACAGGA‑TGTACGGGAAGAGACAAG‑GGGGGCTTTCCGGCCAAGCAGGCTGGGGGGCTTC‑CGGGTA‑AATGTTC‑CCC‑TT‑AGCG‑GTG‑AAGTGTTTTCCCCCGCACCCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGc < 1:179686/147‑1 (MQ=255) aaaaaaCGGGA‑TGTACGGGAAGAGCAATT‑GGCGCTATCCCGGACCAGCAACGTTGG‑GGCATTGCCGGTA‑AAATTTC‑CCC‑TC‑ACCC‑GGG‑AGGTTTTTTCCCCCGCACCCGGT‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCg < 1:441290/145‑1 (MQ=255) aaaaaaCAGGA‑TGTACGGGAAGAGCAAAG‑GGCGGCATTCCGGACCAGCAAGCTTGGCGGCTTT‑CGGGTA‑AAATTTC‑CCC‑TT‑AGCC‑GTA‑AAGAGTTTTCCCCCGCACCCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGa < 1:1126533/148‑1 (MQ=255) taaACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAAc > 2:489532/1‑151 (MQ=255) accaggtttttcTGGAAGAGCAAGG‑GGGGGCATTCCTGACCAGCAAGCTGGGCGGCATC‑CGGGAA‑AAATTTT‑CCT‑TG‑AGCC‑TGA‑AAGGGTTTTCCCTCGCACCCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACcg < 1:1034121/140‑1 (MQ=255) ggA‑TGTACGGGAAGACCCAAG‑GGCGGCTTTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTA‑AATTTTC‑CCC‑TT‑AGCC‑GTG‑AAGAGTTTTTCCTCGCACCCCGT‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACcgc < 1:187249/148‑1 (MQ=255) gtttGTACGGAAAGACCCAGT‑GGCGCCA‑TCCCGACCCGGCAACTTGGGCGCCAT‑CCGGGT‑AAATTTT‑CCC‑TG‑TGCC‑GGGAAAGAGTTTTCCCCCCCACCCCAA‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACcgc < 2:1097899/145‑1 (MQ=255) tACTGGAAGAGCAAAG‑GGCGGCTTTCCGGACCAGCAAGCTTGGCGGCATT‑CCGGTA‑AAATTTC‑CCC‑TT‑AGCC‑GTG‑AAGGGTTTTTCCCCGCACCCCGT‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGaa < 1:884312/150‑1 (MQ=255) aCTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAAc > 1:27894/1‑150 (MQ=255) tGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACAtt > 1:249186/1‑151 (MQ=255) aaGAGCAAAG‑GGCGGTATTCCTCACCAGCAAGTTTGGGGGCATT‑CGGGTG‑AAAGTTC‑CCC‑TT‑ACCC‑GTA‑AAGAGTTTTCCCCCGCACCCCGT‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGa < 1:871244/150‑1 (MQ=255) aaggCCAATGGGCGCCATCCCTGACCAGCAGGTTGGGCGGCTTC‑CGGGTA‑AAATTTC‑CCC‑TT‑CGCC‑GTA‑AAGAGTGTTCCCCCCCACCCCTT‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACa < 1:411798/143‑1 (MQ=255) gccaggggcgccATCCCGGACCAGAAGGTTTGGCGCCTTT‑CCGGTA‑AAATTTC‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCCCGCACCCCGT‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGaaa < 2:579194/142‑1 (MQ=255) aagggcggcTTCCCTGACAAGAAAGTTTGGGGGCTTT‑CGGGTA‑AAATTTC‑CCT‑TT‑AGCG‑GTA‑AAGAGTTTTCCCCCGAACCCCGT‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTtat < 1:779240/148‑1 (MQ=255) t‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTtat > 1:583972/1‑149 (MQ=255) aTTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGAt > 2:1017655/1‑151 (MQ=255) cTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTc > 2:526867/1‑151 (MQ=255) tGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCt > 1:295689/1‑151 (MQ=255) aCCAGCAGGCTTGGCGTCTTC‑CGGGTG‑AATTTTC‑CCT‑TT‑AGCC‑GGA‑AAGTGTTTTTCCCCGAACCCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCt < 2:1101868/149‑1 (MQ=255) agaaaGCTGGGGGGCATC‑CCGGTG‑AATTTTC‑CCC‑TG‑AGCC‑GTG‑AAGAGTGTTTCCTCGCACCCCGT‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCt < 2:929307/143‑1 (MQ=255) ggcggcctgcGGTGAAAAGTTC‑CCC‑TT‑TTCC‑GTG‑AAG‑TTTTTTCCCCGACACCCGTAACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGgc < 1:657803/141‑1 (MQ=255) ggcgcgattcgggtaaaTTTT‑CCCTTT‑CGCC‑GTG‑AAGGGTTTTTCCCCGCACCCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCgg < 2:912845/135‑1 (MQ=255) cATT‑CCGGTG‑AATTTTT‑CCC‑TT‑GGCC‑GTG‑AAGAGTGTTTCCCCGCACCCCGT‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTAt < 2:1107133/151‑1 (MQ=255) ggaaaatgTTC‑CCC‑TT‑AGCC‑GTG‑AGGATTTTTCCCCCGCACCCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGc < 2:1130554/140‑1 (MQ=255) gaaaaaTTT‑CCC‑TTGCGCC‑GAG‑AAGGTTTTTCCCCCCCACCCCGA‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCa < 2:502056/145‑1 (MQ=255) aaaGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACg > 2:326499/1‑151 (MQ=255) aaGTTTCCCC‑TT‑GGCC‑GGG‑AAGGGTGTTCCCCCCCACCCCGC‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCa < 1:1072780/149‑1 (MQ=255) aaGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACgg > 2:1035388/1‑151 (MQ=255) gTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACggg > 2:586424/1‑150 (MQ=255) t‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATc > 2:458626/1‑151 (MQ=255) cc‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCt > 1:198262/1‑151 (MQ=255) cc‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCt > 2:665491/1‑151 (MQ=255) aaggtttttcccccGCACCCCTT‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTCCTg < 1:538515/137‑1 (MQ=255) ttttcccgcGCACCCCTA‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGc < 2:1015718/138‑1 (MQ=255) tCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGa > 2:708179/1‑150 (MQ=255) ccccGCACCCCGT‑CCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCggggggg < 1:748122/145‑1 (MQ=255) ccccGCACCCAGT‑GCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCgggggg < 2:267490/144‑1 (MQ=255) cGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATaa > 1:642439/1‑150 (MQ=255) gCAACACGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATaa > 2:389988/1‑149 (MQ=255) | GTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGA‑TGTACTGGAAGAGCCAAT‑GGCGGCATTCCTGACCAGCAAGCTTGGCGGCATT‑CCGGTG‑AAAGTTT‑CCC‑TT‑AGCC‑GTG‑AAGAGTGTTTCCTCGCAACCCGT‑ACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAA > NC_000913/3001020‑3001309 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |