Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 436,516 | 0 | C | A | 44.1% | 85.3 / 25.4 | 59 | Q295K (CAA→AAA) | thiL | thiamine monophosphate kinase |
Reads supporting (aligned to +/- strand): ref base C (30/3); new base A (0/26); total (30/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 9.23e-14 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 |
TCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCT > NC_000913/436369‑436663 | tctcGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCCCTGGCGGTGAAGCTTACGCGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGCGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCa > 2:590469/1‑149 (MQ=255) ttGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGAccgccg > 2:778539/1‑150 (MQ=255) aCCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATc > 2:162061/1‑151 (MQ=255) ccGGACCAGGCGCTGCGCTGGGCGCTCTCGGGCGGTGAAGATTACGAGTTGTGTTTCCCTGTGCCGGAACTAACCCGTGGCGCGCTGGATGTGGCTCTCGGACCCCTGGGCGTCCGGTTTACCTGTAGCGGGAAAATGACCGCCGATATc < 2:193629/150‑1 (MQ=255) ggAACGGGCGCGGCGCGGGGCGCTCTCGGGGGGGGAAGATTCCGAGTTGTTTTTCACTGTGCCGGAACTGACCGGTGGCGCGCTGGAGGTGGCTCTCGCACCCCTGGGCGTCCCTTTTACCTGTATCGGGAAAATGACCGCCGATATCGaa < 2:160610/151‑1 (MQ=255) gctgcgctgGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGt > 2:882878/1‑151 (MQ=255) tgcgctgGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGttt > 2:788637/1‑151 (MQ=255) gcgctgGGCGCTCTTGGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGGGGCGCGCGGGATGTGGCTCTCGGACACCTGGGCGTCCCGTTTCCCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGtt < 1:131500/149‑1 (MQ=255) cgctgGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGttt > 2:739771/1‑149 (MQ=255) gctgGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTAt > 2:225908/1‑151 (MQ=255) ctgGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTAtt > 1:12403/1‑151 (MQ=255) gggCGCTCTCTGGGGGTAAAGATTACGAGTTGTTTTTCCTTGGGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCGGGGCGTACCGTTTCCTTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTAtt < 1:256593/149‑1 (MQ=255) ctctGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGc > 2:222455/1‑151 (MQ=255) tGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGa > 2:57384/1‑150 (MQ=255) gggggTGAAGTTTAGGATTTTTGTTTCCCTGTGCGGGAACTGACCGGGGGCGGGCTGGATGTGGCTCTCGGACCCCGGGGCGTCCTTTTTACTTGTGGCGGGAAAAGGCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCg < 2:538332/145‑1 (MQ=255) ggagAAGATTCGGGGTTGTGTTTCAGGGTGCGGAAACTGACCCGTGGCGGGCTGGTTGGGGCTCTGGGCCACCTGGGCTTCCCTTTTCCCTGTAGCGGACAAAGAACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTg < 2:304454/148‑1 (MQ=255) ggTGAAGTTTACGATTTGTGTTTCCCTGTGCCGGAACTGAACGGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTCCCTTTTCCCTTTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCt < 1:394945/150‑1 (MQ=255) ggTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTg > 1:599293/1‑151 (MQ=255) ggTAAAGATTACGAGTTGTGTTTCATTGTGCCGGAACTGACCCGGGGCGCGTTGGATGTGGCTCTCGGACACCTGGGCGTCCCGTTTCCTTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCt < 1:954066/150‑1 (MQ=255) tGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGtt > 1:1067314/1‑151 (MQ=255) agatttACGGGTTGTTTTTCCCTGGGCGGAAACGGACCCGGGGCGCGTTGGTGGGGGCTCGCGGCCCCCGGGGCGTTCTGTTTCCTTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTa < 2:413660/146‑1 (MQ=255) aaGATTACGAGTTGTGTTTCACTGTGCCGGAGCTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTAc > 2:236118/1‑151 (MQ=255) gAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACt > 1:670552/1‑151 (MQ=255) gAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACt > 2:880873/1‑151 (MQ=255) aGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTg > 1:902150/1‑151 (MQ=255) tgtgTTTCCCTGTGCCGGAACGGACCCGTGGCGCGTTGGAGGTGGCTCTCGGACCCCTGGGCGTACCGTTTCCCTTTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGa < 1:322700/150‑1 (MQ=255) tgtTTCCCTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCGGGGCGTCCCTTTTACCTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGaaa < 1:295275/150‑1 (MQ=255) gtTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAgg > 1:600668/1‑151 (MQ=255) tttCACGGTGCGGGAACGGACCCGGGGCGCGTGGGTTGTGGCTCTCGGACCCCGGGGCGTCCCGTTTCCCTGTACCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGa < 1:191260/146‑1 (MQ=255) aCTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATg > 2:519238/1‑151 (MQ=255) ccGGACCTGACCCGTGGCGCGTTGGTTGTGGCTCTCGGCCCCCGGGGGGTCCCTTTTACCTTTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCAtt < 1:1096157/151‑1 (MQ=255) ccGGAACTGACCCGTGGCGCTTTGGATGTGCCTCTCGGACCCTTGGGCGTCCCTTTTACTTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCAtt < 2:161974/151‑1 (MQ=255) tGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCAc > 1:757212/1‑151 (MQ=255) gaggcgctttggcggtgGCTCTGGGCCACTGGGGCGTCCGTTTTACTTGTTCCGGGAAAAGAACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACg < 2:914689/132‑1 (MQ=255) gTGGCGCGTTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTCCCTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCAc < 1:575704/145‑1 (MQ=255) ggCGCGTGGGGTGGGTCTCTCGGACCCCTGGGCGTCCCTTTTCCTTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATaa < 2:815530/150‑1 (MQ=255) ggCGCGCTGGTTGTGTCTCTCGCCCTCCTGGGCGCCCCTTTTACCTGTAGCGGAAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCa < 1:500586/147‑1 (MQ=255) ggCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATaa > 2:410973/1‑150 (MQ=255) gcgcgcGGGATGTGCCTCTCGGCCACCGGGGCGTACCTTTTCCCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAg < 1:383226/151‑1 (MQ=255) gttGGATGGGGCTCCGGGCCCCCGGGGGGTCCCTTTTCCTTGTATGGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATg < 1:711242/148‑1 (MQ=255) tGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTcg > 1:106965/1‑151 (MQ=255) ggggCTCCGGGACCCCGGGGCGTCCCTTTTCCCTGTTCGGGGAAAAGGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGt < 1:909596/142‑1 (MQ=255) ggCTCTGGGTCACCGGGGGGTTCCTTTTACCTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAg < 1:364367/151‑1 (MQ=255) tcGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGcc > 2:1025904/1‑151 (MQ=255) cGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCt > 2:595014/1‑151 (MQ=255) ccgcTGGGCGTCCCTTTTACCTTTAGCGGGAAAAGGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGCCTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGc < 2:152522/142‑1 (MQ=255) acCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGa > 1:180502/1‑148 (MQ=255) ggggCGTACTTTTTACCTGTAGGGGAAAAAGGGCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGa < 2:289550/150‑1 (MQ=255) gtccgttttgcttGTATGGGAAAAAAGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAAt < 1:241459/139‑1 (MQ=255) tACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATc > 2:186563/1‑151 (MQ=255) ccGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCg > 1:114804/1‑151 (MQ=255) tttccttttttggggAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGt < 1:528320/137‑1 (MQ=255) tttccttgattCGGGAAAAGTACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTg < 2:422863/140‑1 (MQ=255) tttACCTGTAGCGGAAAAATAACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTg < 2:982610/150‑1 (MQ=255) aCCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCAt > 1:1108042/1‑151 (MQ=255) tGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCt > 1:98486/1‑150 (MQ=255) tCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTg > 2:123611/1‑151 (MQ=255) gggCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCt > 2:11246/1‑151 (MQ=255) gggAAAAAGTCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACtt < 2:393277/148‑1 (MQ=255) | TCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCT > NC_000913/436369‑436663 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |