Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,175	C→G	E48D (GAG→GAC)	glyQ ←	glycine tRNA synthetase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,175	0	C	G	100.0%	317.3 / NA	82	E48D (GAG→GAC)	glyQ	glycine tRNA synthetase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (43/39);  total (43/39)

AGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTA  >  NC_000913/3725029‑3725322                                                                                                                                                   |                                                                                                                                                    aGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTcgc                                                                                                                                                 >  1:74511/1‑151 (MQ=255) aGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTcgc                                                                                                                                                 >  2:67024/1‑151 (MQ=255)  acTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTcgcg                                                                                                                                                >  2:275715/2‑151 (MQ=255)      cTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCgcgc                                                                                                                                               >  2:424496/1‑148 (MQ=255)        gAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGAc                                                                                                                                           >  2:970668/1‑150 (MQ=255)        gAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACa                                                                                                                                          <  2:295031/151‑1 (MQ=255)         aaTATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGAc                                                                                                                                           >  1:831005/1‑149 (MQ=255)         aaTATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGAc                                                                                                                                           >  2:480260/1‑149 (MQ=255)            atTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGt                                                                                                                                       <  1:528735/150‑1 (MQ=255)               gTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCAt                                                                                                                                    <  1:94306/150‑1 (MQ=255)                   ggCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGt                                                                                                                               >  1:522664/1‑151 (MQ=255)                    gCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTg                                                                                                                              <  2:1043978/151‑1 (MQ=255)                      gATGGCTTAATGACCACCTGGACCTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTg                                                                                                                              >  2:193465/1‑149 (MQ=255)                      gATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTg                                                                                                                              >  1:46556/1‑149 (MQ=255)                             tAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGtt                                                                                                                       <  1:539913/149‑1 (MQ=255)                                   ccaccTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGcc                                                                                                               <  1:460464/151‑1 (MQ=255)                                      ccTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGAc                                                                                                            <  1:1068732/151‑1 (MQ=255)                                          gAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTcc                                                                                                        <  1:338508/151‑1 (MQ=255)                                          gAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTcc                                                                                                        <  1:594957/151‑1 (MQ=255)                                           aaCTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTcc                                                                                                        >  2:664907/1‑150 (MQ=255)                                           aaCTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCa                                                                                                       <  2:483139/151‑1 (MQ=255)                                            aCTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCa                                                                                                       <  2:194562/150‑1 (MQ=255)                                              tGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGt                                                                                                    >  1:5198/1‑151 (MQ=255)                                              tGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCGGGCCCCAGGCCGCGCAGACAGGTCATTGGGGGAGAGGTTCCCGCGCCGACTTCCATg                                                                                                     >  2:363403/1‑150 (MQ=255)                                               gATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATg                                                                                                     <  1:459742/149‑1 (MQ=255)                                               gATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGCGAGAGGTTCCCGCGCCGACTTCCATGTc                                                                                                   >  2:178846/1‑151 (MQ=255)                                                aTAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTcc                                                                                                  <  2:997518/151‑1 (MQ=255)                                                aTAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTcc                                                                                                  >  2:728417/1‑151 (MQ=255)                                                aTAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTcc                                                                                                  <  1:820574/151‑1 (MQ=255)                                                aTAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGGTGGGCGACGAGAAGGCTGAACATAAGCAGTCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACCGGTCCTTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTcc                                                                                                  <  2:784549/151‑1 (MQ=255)                                                 tagtagTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTcc                                                                                                  >  1:618616/1‑150 (MQ=255)                                                    tagtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATg                                                                                              >  1:126508/1‑151 (MQ=255)                                                     agtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATgg                                                                                             <  2:563957/151‑1 (MQ=255)                                                      gtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGt                                                                                            >  1:225480/1‑151 (MQ=255)                                                      gtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGt                                                                                            >  2:343740/1‑151 (MQ=255)                                                              aaCGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAAt                                                                                    <  1:30022/151‑1 (MQ=255)                                                               aCGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATg                                                                                   <  1:751700/151‑1 (MQ=255)                                                                cGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATgg                                                                                  <  2:751932/151‑1 (MQ=255)                                                                cGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATgg                                                                                  <  2:28597/151‑1 (MQ=255)                                                                cGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATgg                                                                                  >  1:631634/1‑151 (MQ=255)                                                                   ttGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTg                                                                                <  2:603442/150‑1 (MQ=255)                                                                   ttGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGc                                                                               >  1:180328/1‑151 (MQ=255)                                                                   ttGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGc                                                                               >  2:826420/1‑151 (MQ=255)                                                                       ggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGcc                                                                           <  1:832611/151‑1 (MQ=255)                                                                       ggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGcc                                                                           >  2:323856/1‑151 (MQ=255)                                                                        gTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGccc                                                                          >  1:867133/1‑151 (MQ=255)                                                                        gTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGccc                                                                          <  2:320308/151‑1 (MQ=255)                                                                          tttCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCt                                                                         <  1:552910/150‑1 (MQ=255)                                                                           ttCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTg                                                                        <  1:967236/150‑1 (MQ=255)                                                                           ttCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTg                                                                        <  2:815256/150‑1 (MQ=255)                                                                            tCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTgg                                                                       <  1:523315/150‑1 (MQ=255)                                                                              gCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCg                                                                     >  1:389092/1‑150 (MQ=255)                                                                              gCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGa                                                                    <  2:479567/151‑1 (MQ=255)                                                                                   aGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGccc                                                                >  1:960475/1‑150 (MQ=255)                                                                                     cGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGt                                                             <  2:194510/151‑1 (MQ=255)                                                                                      gACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGt                                                             >  2:178713/1‑150 (MQ=255)                                                                                              gtcggGGGGCGAGAAGGCTGAACATAAGCCGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGt                                                     <  2:533614/150‑1 (MQ=255)                                                                                              gtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGt                                                     >  1:418750/1‑150 (MQ=255)                                                                                               tcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTa                                                    >  2:173609/1‑150 (MQ=255)                                                                                                     gacgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAg                                             <  2:221332/151‑1 (MQ=255)                                                                                                      acgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAgg                                            >  2:752267/1‑151 (MQ=255)                                                                                                        gagaAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGAt                                          >  1:1115504/1‑151 (MQ=255)                                                                                                          gaAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATc                                         >  2:295664/1‑150 (MQ=255)                                                                                                           aaGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCa                                        >  1:885183/1‑150 (MQ=255)                                                                                                             ggCTGAACATAAGCAGCCGCCATCGGTTCTGG‑CCCAGGGCGCGCAAAACGGGCATTGGGGGGGGGGGTTCCGCGCCGACTTCCATGTCCCATGGGTGAACAATGGGGCAACCCTGGCCAGCCCAGTAATCCTGTAAGGTCAGGATCaaagc                                    >  1:1108694/1‑148 (MQ=255)                                                                                                                 gAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTg                                 >  1:304440/1‑151 (MQ=255)                                                                                                                 gAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTg                                 <  2:699106/151‑1 (MQ=255)                                                                                                                         gcagcCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTcc                         >  1:461307/1‑151 (MQ=255)                                                                                                                          cagcCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCt                        >  1:870233/1‑151 (MQ=255)                                                                                                                           agcCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGTACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGGCCTg                       >  2:104681/1‑151 (MQ=255)                                                                                                                           agcCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTg                       <  2:950989/151‑1 (MQ=255)                                                                                                                             ccgccATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGt                     <  1:796500/151‑1 (MQ=255)                                                                                                                               gccATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTAt                   <  1:702351/151‑1 (MQ=255)                                                                                                                                ccATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTAt                   >  2:317765/1‑150 (MQ=255)                                                                                                                                 cATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGACAGGATCAAGCCCTGGAAGGTCCTGGTATc                  >  2:1141290/1‑150 (MQ=255)                                                                                                                                   tCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCaa                >  2:547335/1‑150 (MQ=255)                                                                                                                                    cGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCaaa               <  2:799581/150‑1 (MQ=255)                                                                                                                                     ggTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACt             >  2:905159/1‑151 (MQ=255)                                                                                                                                      gTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACtt            <  1:390095/151‑1 (MQ=255)                                                                                                                                           ggCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGc        >  1:48259/1‑150 (MQ=255)                                                                                                                                             ccccAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGc        <  1:892482/148‑1 (MQ=255)                                                                                                                                                cAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCAtatta  <  1:1118210/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    AGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTA  >  NC_000913/3725029‑3725322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑