| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,478,324 | 0 | T | G | 46.3% | 76.1 / 23.8 | 55 | G33G (GGT→GGG) | ynbB | putative CDP‑diglyceride synthase |
| Reads supporting (aligned to +/- strand): ref base T (7/22); new base G (25/0); total (33/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACA > NC_000913/1478176‑1478470 | aCTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGa < 2:227662/151‑1 (MQ=255) gATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGGGGAAAAGGGGGGGG‑GGt > 2:599989/1‑150 (MQ=255) ttAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGGAAAGGGGGGGA‑gg > 2:980389/1‑146 (MQ=255) aTCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTa < 1:401183/151‑1 (MQ=255) aTCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTa < 1:640475/151‑1 (MQ=255) gTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTAtt < 2:266656/151‑1 (MQ=255) cGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTc < 2:749403/150‑1 (MQ=255) cGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTc < 2:626113/150‑1 (MQ=255) cttATGCGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCT‑CGTGCGGAGAGGGGGCGA‑GGTTACCTTAATTATTCCTTCATTGTgggg > 2:58250/1‑151 (MQ=255) ttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATC‑GGAGAGAAAAAGGGGGGG‑GGTTTCATTACGTATTTGTAACTGGTgggg > 1:509154/1‑150 (MQ=255) aTGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCAt < 2:627309/151‑1 (MQ=255) cgcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGGGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCg > 2:247049/1‑148 (MQ=255) gcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATA‑GGGGAGCAAAGGGGGGGA‑GGGAACATTTCGCAGTCGTATCTGTGgggggtta > 1:739571/1‑142 (MQ=255) cTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAAC‑GGGGAGAAAAGGGGGGGA‑GGGTACATTATGTATTTGTACCTGGTGGGGGATCGTCCtt > 2:515308/1‑151 (MQ=255) tGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGGTACC‑GGGGAGAAAAGGGGGGGG‑GGTTACATTACTGATTTGTTTCTTGTGGGGGATCGTCAttt > 1:606128/1‑151 (MQ=255) aaaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGGGAGAAAAGGGGGGGA‑GGGTTCCTTACGTATTTGTACCTGGGGGGGGGTCGTCAttt > 1:602540/1‑149 (MQ=255) taATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGGGG‑GGGTACATTAAGTATTTGTACCTGGTGGGGGATCGGGCTTTGtttttt > 2:560264/1‑150 (MQ=255) tGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCC‑GGGGAGAAAAAGGGGGGG‑GGGTACATTACGTATTTGTACCTGGTGGGGGGTCGTCATttttttttttat > 1:472352/1‑147 (MQ=255) gAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑g < 1:230570/150‑1 (MQ=255) aaaaaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCC‑CGAGAGAAAAAGGGGGGA‑GGGTAAATTTCCTATTTGTACCTGGGGGGGGATCGTCCTTTTTTTTTCTATtgt‑gg > 1:715563/1‑149 (MQ=255) aaaaaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATC‑CGGGAGAAAAAGGGGGGG‑GGGTAAATTACGTATTTGTGCCCGGTGGGGGATCGGCCTttttttttttat > 1:616394/1‑141 (MQ=255) aaaaaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGGGAGAAAAGGGGGGGG‑GGTTTCATTACGTATTTGTACCTGGTGGGGGATCGTCATTTTTTTTTCTATgggg > 2:154463/1‑147 (MQ=255) aaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGGGA‑GGTTACATTACGTATTCGTACCTGGGGGGGCATCGTCATTTGTTTTTTAATGGT‑GAttt > 2:570596/1‑150 (MQ=255) ctctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAAC‑GGGGAGAAAAAGGGGGGG‑GGTTTCATTACGTATTCGTACCTGGTGGGGGCTCGGCATTTGTTTTTTTATGGG‑GATTTTAgg > 2:653997/1‑151 (MQ=255) ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAg < 2:814816/148‑1 (MQ=255) ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAg < 2:506435/148‑1 (MQ=255) gcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCC‑GGGGGGAAAAGGGGGGGG‑GGGTAAATTATGTATTTGGAACCGGTGGGGGCTCGGCCATTTTTTTTCCATTGT‑GATTTTTGGGCCAAGCCCGGtgacgt > 1:97004/1‑151 (MQ=255) tgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATC‑GGGGAGAAAAGGGGGGGG‑GGTTTCATTTCGTATTTGTAACTGGTGGGGGATCGTCATTTTTTTTTTTATGGG‑GGTTTTAGGGCCACGCCGGGtgacgtt > 1:200137/1‑148 (MQ=255) gttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGAtgacgttgacg < 2:520727/151‑1 (MQ=255) ttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGATACA‑AGGGAGAAAAGGGGGGGA‑GGGTTTATTTTGTATTTGTTCCTTGTGGGGCATCGTTATTTTTTTTTTTATGGT‑GATTTCATGCACACGCTCGATGACGATGACGtttttt > 1:180014/1‑150 (MQ=255) gCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTgcgc < 2:213143/151‑1 (MQ=255) gCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑CGGGAGGAAAGGGGGGGG‑GGGTTCATTTAGTATTTGTTCCTCGTGGGGGATCGTCATTTTTTTTTTTATGGT‑GGTTTTATGTCCCCGCTGGGTGGCGTTTACGtttttt > 1:417590/1‑147 (MQ=255) cAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAAC‑GGAGAGAAAAGGGGGGGAGGGTTAAATTA‑GTATTCGTACCCGGGGGGGGATCGGGCTTTGTTTTTCTATGGG‑GATTTTAGGGCCCCGCTGGGTGGCGTTTGCGgtttttt > 1:167561/1‑146 (MQ=255) aaCGCTGATAAACCGTTTTCTGCTATGGCGGTTTCC‑GGGGAGAAAAGGGGGGGG‑GGGTTAATTTAGTATTCGTACCTCGGGGGGGGTCGGCATTTTTTTTTTTATGGTGGTTTTCAGTGCCA‑GCTGGGGGAAGTTGACGtttttt > 2:244809/1‑145 (MQ=255) aaCGCTGATAAACCGTTTTCTGCTATGGCGGTTTCC‑GGAGAGAAAAAGGGGGGG‑GGGTACATTACCTATTTCTACCTGGTGGGGGATCGGTATTTTTTTTTTTCTGGT‑GGTTTCCGGCCCACGCCGGGTGACGTTGAAGtttttt > 2:12786/1‑145 (MQ=255) gCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATa < 1:176465/151‑1 (MQ=255) gATAAACCGTTTTCTGCTATGGCGGTTACC‑GGGGAGAAAAGGGGGTGG‑GGGTACATTTCGTATTTGTTACCGGGGGGGGAGCGGCATTTGTTTTTTAATGGG‑GATTTTAGGTCCACGCTGGGTGACGGTGACGGTTTTTTTGCTgtt > 1:514598/1‑145 (MQ=255) gATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGGGA‑GGTTACATTACGTATTCGTACCTGGGGGGGGATCGTCATTTTTTTTTTAATGGT‑GATTTTAGGTCCACGCTGGGGGGCGTTGACGTTTTTTTTGCTGTTAAg > 1:498247/1‑150 (MQ=255) aTAAACCGTTTTCTGCTATGGCGGTTACC‑GGGGAGAAAAGGGGGGGA‑GGGTACATTACGTATTTGTACCTGGTGGGGGATCGTCATTTGTTTTTTAATGGG‑GATTTCAGGTCCACGCTGGGTGACGTTGACGTTTTTTTCGCTGGTAAggt > 2:871919/1‑149 (MQ=255) aTAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGtt < 2:806859/151‑1 (MQ=255) cGTTTTCTGCTATGGCGGTTACC‑GGGGAGGAAAAGGGGGGG‑GGGTACATTACGGATTCGTACCTGGGGGGGGGTCGTCATTTGTTTTTCAATGGT‑GTTTTCAGGTTCACGCCGGGTGACGTTGGCGTTTTTTTTGCTGATAAGTTTTCt > 2:66583/1‑149 (MQ=255) tCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCAtt < 2:913989/150‑1 (MQ=255) gCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGa < 1:488474/149‑1 (MQ=255) aTGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGa < 2:484017/150‑1 (MQ=255) ggTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTg > 2:383680/1‑151 (MQ=255) ggAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCtt > 2:738029/1‑151 (MQ=255) ggAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCtt > 2:34907/1‑151 (MQ=255) gagaAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtata < 2:472437/151‑1 (MQ=255) gaAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATc > 1:526386/1‑151 (MQ=255) aaaaGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCt < 2:752517/151‑1 (MQ=255) aaaGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTg < 1:25582/151‑1 (MQ=255) aaGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGt < 2:514148/151‑1 (MQ=255) aGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGt > 1:298174/1‑150 (MQ=255) ggggTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGTCGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACa < 1:560170/151‑1 (MQ=255) ggggTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTAc > 2:606259/1‑150 (MQ=255) | ACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGA‑GGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGT‑GATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACA > NC_000913/1478176‑1478470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |