| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 17,000 | 0 | C | T | 40.0% | 60.1 / 17.0 | 45 | intergenic (‑40/‑489) | mokC/nhaA | regulatory protein for HokC, overlaps CDS of hokC/sodium‑proton antiporter |
| Reads supporting (aligned to +/- strand): ref base C (19/8); new base T (0/18); total (19/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.10e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAT > NC_000913/16851‑17146 | gcggTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCt < 2:971359/151‑1 (MQ=255) gATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTtct > 1:136157/1‑151 (MQ=255) aCAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTa < 2:861288/151‑1 (MQ=255) cAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTc > 2:281560/1‑149 (MQ=255) aGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGc > 1:865861/1‑151 (MQ=255) ggCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTa < 1:369123/151‑1 (MQ=255) aCAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGc > 2:41911/1‑151 (MQ=255) aaTCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCt < 2:640746/151‑1 (MQ=255) aaTCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCt < 1:374868/151‑1 (MQ=255) tATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGagacagac < 2:867642/149‑1 (MQ=255) tATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGagacagac > 1:281174/1‑149 (MQ=255) tCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGc > 2:251982/1‑150 (MQ=255) cATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCAc > 1:634179/1‑151 (MQ=255) ctctctTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACccgcc > 2:100991/1‑151 (MQ=255) tGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCg > 2:896706/1‑151 (MQ=255) tGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCg > 2:699281/1‑151 (MQ=255) gACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGTCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCCCCCGCCGCTATTATATc < 2:633517/149‑1 (MQ=255) gcGGTGAGTAAGAGCCATTCAACTTTTTTTCACAATATAGGACGCGTGGGGTTGATAAAAAAATCCCTCTGGGGTTTTTTCTCTACCTGCCGTTCTGCTAA‑TCCCGAAAACAGACAGCCTAAACCCCCCGCCGCTATTATATCGctct < 1:325523/147‑1 (MQ=255) aaGAGGCACTCTACATGTGTTCAGAATAGAGGAGGCCTGGGGTTGATGGAAAAATACCCCTGGGGTTTTTTCTCTATCTCCCTTTCAGTTAA‑TGCCTGAGACAGACACCCCCAACCCCCCGCCGCTATTATATCGCTCTCTTTAAcc < 2:684308/147‑1 (MQ=255) aaGAGCCCCTCTACATTTTTTCAGCAAATAGGAGCCCTGGGTTTGATGTAAAAATACCCCTGGGGGTTTTTCTCATTCTGCCGTTAAGTTAA‑TGCTGGAGACAGACACCCTCAACCACCCGCCGCTATTATATCGCTCTCTTTaa < 2:34363/145‑1 (MQ=255) aaGAGCCACTCTACATGTGTTCAGAAAATAGGAGGCCTGGGGTTGATGGTAAAATACCACCCGGGTTTTTTCTCTATCGCCCGTTCAGCTAA‑TGCCTGAGACAGACACCCTAAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCa < 2:398154/149‑1 (MQ=255) gagGCACTCTACATTTGTTCAGCATAGAGGGGCCCTCGGGTTGATGATAAAATACCCCGCGGGGTTTTTCTCTCTCCGCCGTTAATCTAA‑TGCCTGAAACAAACACCTACAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCAttt < 1:941537/150‑1 (MQ=255) gagGCACTCTACATGTTTTCAGAATAAGGGGGGCCTGGGTTTGATGGAAAAATATCCCTCGGGTTTTTTCTCTATCTGCCTTTCAGCTAA‑TCCCTGAGACAGCCACCCCAAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCAttt < 2:325944/150‑1 (MQ=255) ccctctACATGTGTTCAAAATATAGGAGGCCTGGGGTTGATGGAAAAATTTCCCTCGGGGTTTTTCTTTATCTGCCGTTCAGCTAA‑TGCCTGAGAAAGACAGCCACAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGt < 2:859511/147‑1 (MQ=255) cATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGAt > 2:591107/1‑151 (MQ=255) tgtgTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTc > 2:427271/1‑151 (MQ=255) gtgtTCATCATATAGGGGGCCTGGGTTTGGTGAAAAAATCTCCCGCGGGTTTTTTCTCTCCCTCCCTTTCAGCTAA‑TCCCTGAGACAGACACCTTAAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCg < 1:528962/146‑1 (MQ=255) aggaggCTTGGGGTTAAGGAAAAAATACCACTGGGGGCTTTTCTCTATCCCCCGTTCAGTTAA‑TGCCTGAAACAGCCCCCCTCAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCt < 1:641941/138‑1 (MQ=255) ggCCTCGGTTGGATGATAAAATATCCCTGGGGGTTTTTCTCTATCCGCCGTTCATCTAA‑TCCCTGAGACAGACACCCTCAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTc < 1:279673/151‑1 (MQ=255) ggCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTc > 1:593301/1‑151 (MQ=255) cctgggggtgaagaaaaaaTATCACGCGGGGTTTTTCTCTATCTGCCGTTCA‑CTAATTCCCAGAGACAGACCCCCCAAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGc < 2:892216/136‑1 (MQ=255) ccTGGGTTTTATGATAAAATATCCCGGGGCGTTTTTCTCTATCTGCCTTTCATCTAT‑TGCCTGAGACCAACTGCCTCAACCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGAc < 2:237609/144‑1 (MQ=255) gggTTAAGGTTAAAATATCGTTGGGGGTTTTTCTTTTCCTCCCTTTCATCTAT‑TGCCGCAGAAAGACACCCTAAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAttt < 2:739484/151‑1 (MQ=255) tggatgaaaaaaTATCCCTGGGGCTTTTTCTCTACCGGCCTTTCACCTAA‑TCCCTGAGACAGACACCCTCAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAttttt < 1:717027/142‑1 (MQ=255) tGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGc > 2:160169/1‑151 (MQ=255) gTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGt > 2:146980/1‑151 (MQ=255) aaaTACCCCCCGGGGTTTTTCTTTACCTCCCGTTCAGCAAA‑TGCCGGAGACAGACACCCTCAAGCCACCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAAt < 2:71506/151‑1 (MQ=255) atatCCCTCGGGCTTTTTCTCTATCTGCCTTTAAGTTAA‑TGCCTGAGACAGACCCCCTAAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAAttt < 1:236735/151‑1 (MQ=255) aCTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTa > 2:349397/1‑150 (MQ=255) ccgggggTTTTTCTCTTTCTGCCTTTCATCTAA‑TCCTTGAGACAACCACCCTCAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAAt < 1:740415/148‑1 (MQ=255) cTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAAt > 1:312677/1‑151 (MQ=255) tCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATg > 1:543206/1‑151 (MQ=255) ggggTTTTTCTTTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACACCCTCAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAt < 2:281225/151‑1 (MQ=255) ggggTTTTTCTCTATCTGCCGTTAAGCAAA‑TGCCGGAGACAGACACCCTCAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAt < 2:439363/151‑1 (MQ=255) ggggCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAt > 2:208330/1‑151 (MQ=255) | GCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAA‑TGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAT > NC_000913/16851‑17146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |