Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,261,055	0	A	C	44.4%	98.1 / 12.1	63	V455G (GTG→GGG)	tdcE	pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (30/5);  new base C (0/28);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 5.91e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTC  >  NC_000913/3260905‑3261200                                                                                                                                                       |                                                                                                                                                  aTGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCa                                                                                                                                                   <  2:832336/151‑1 (MQ=255)           tGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCATCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGtt                                                                                                                                         >  2:671773/1‑151 (MQ=255)             atatTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTa                                                                                                                                        >  1:347487/1‑150 (MQ=255)                ttCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTg                                                                                                                                    >  1:754526/1‑151 (MQ=255)                  cAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCg                                                                                                                                  >  1:658998/1‑151 (MQ=255)                     cgcgcTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGt                                                                                                                                 >  1:879939/1‑149 (MQ=255)                         cTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGTCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGagc                                                                                                                            >  2:480292/1‑150 (MQ=255)                         cTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGagc                                                                                                                            >  2:99527/1‑150 (MQ=255)                             tttcctcccccGCCAGCCAGTCCAGGAAGTGATCGAGGCTGTCCATCTCTTTGTCGTAATCCGGCACGTCGTCCATCGGCGGTGTTGTTTTCGGCCCGCCTGGAATTTTCGGTTTCTCGCCCCCCCCGCCGTTAATTGCGTAGagcagc                                                                                                                         <  1:902515/140‑1 (MQ=255)                             tGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGt                                                                                                                       >  1:470850/1‑151 (MQ=255)                              ttccggccccGCCAGCCAGTCCATGAAGTGATCGAGGCTGCCCATCTCTTTGTCGTAACCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGagcagc                                                                                                                         <  2:728645/140‑1 (MQ=255)                               tcccgccccgccgGCCGGCCCATGAGGTGATGGGGGCTGTCCTTCATTTGGTCGTAACCCAGCCGGCCTTCCATCAGCGGTGTTGTTTTCGGCCCGACCAGAATCTTCGGCTTCTCGCCCACCCCGCCGTTAATTGCGTAGagcag                                                                                                                          <  1:256770/139‑1 (MQ=255)                                 cTGCCCCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGtttt                                                                                                                    <  2:425676/150‑1 (MQ=255)                                        gccagccaGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAg                                                                                                              >  2:361746/1‑149 (MQ=255)                                             ccagTCCATGAAGTGATCGAGGCTGTCCATCTTTTTGTCGTAATCCAGCACGTCGCCCATCAGCGGTGCTGTTTTCGCCCCGCCCTAAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTa                                                                                                          <  2:43903/148‑1 (MQ=255)                                             ccagTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCTTAACCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTAAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgc                                                                                                        <  2:242915/150‑1 (MQ=255)                                               agTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcg                                                                                                     >  2:93155/1‑151 (MQ=255)                                                gTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGt                                                                                                    >  1:85565/1‑151 (MQ=255)                                                 tCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTg                                                                                                   >  1:537233/1‑151 (MQ=255)                                                 cccATGAGGTGACCGAGGCTGTCCATCTTTTTGTCGTAATCCAGCACGTCGCCCATCAGCGGTGCTTTTTTCGCCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTg                                                                                                   <  1:694900/150‑1 (MQ=255)                                                  ccATGAGGTGACCGGGGCTTTCCATCCTTTTGTGGTATCCCACCCGGTCGCCCTCCGGCGGTGTTTTTTTCGGCCCGCCTTGATTTTTCACCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgcgc                                                                                                      <  1:444600/147‑1 (MQ=255)                                                       aaGTGATCGAGGCTGTCCATCCCTTTGTCTTAACCCAGCACGCCGTCCATCAGCGGTGTTGTTTTCGCCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCa                                                                                              <  1:490134/150‑1 (MQ=255)                                                        aGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaa                                                                                             >  1:921414/1‑150 (MQ=255)                                                         gTGATCGAGGCTGTCCATCACTTTGTCGTAACCCAGCCCGTCGTCCATCAGCGGTGTTTTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAg                                                                                           <  2:909795/151‑1 (MQ=255)                                                          tGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaa                                                                                             >  2:807726/1‑148 (MQ=255)                                                               gAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGc                                                                                     >  1:350654/1‑151 (MQ=255)                                                                gggCTGCCCATCATTTTGCCGTAACCCAGCACGTCCTCCACCGGCGGTGCTGTTTCGGGCCCGACCTGAATCTTCAGCTCCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGc                                                                                     <  1:132570/149‑1 (MQ=255)                                                                 ggCTGTCCATCATTTTTTCGTAACCCAGCCCGTCGTCCACCGGCGGTGCTTTTTTCGGCCCGACCTGAATTTTCAGTTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGGACTGCa                                                                                    <  2:132905/150‑1 (MQ=255)                                                                 ggCTGTCCATCACTTTGTCGTAACCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCa                                                                                    <  2:757546/150‑1 (MQ=255)                                                                    ttcccATCCCTTTGTCTTAACCCAGCCCTTCGTCCACCGGGGGTGTTTTTTTCGGCCCGACCTGAATCTCCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAtt                                                                                  <  1:914010/146‑1 (MQ=255)                                                                       ccATCTTTTTGTCGTAATCCAGCACGTCGCCCACCGGCGGTGCTGTTTTCGGCCCGACCTAAATCTTCGGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAt                                                                                   <  2:636380/145‑1 (MQ=255)                                                                       ccATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCtt                                                                             >  1:430650/1‑151 (MQ=255)                                                                        catcatTTTGTCGTAATCCAGCACGTCGCCCATCAGCGGTGCTGTTTGCGGCCCGACCTAAATCTTCAGCTTCTCTTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAttt                                                                                 <  1:476082/146‑1 (MQ=255)                                                                              tttGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATc                                                                      >  2:674341/1‑151 (MQ=255)                                                                                 gTCGTAATCCAGCCCGTCGTCCACCGGCGGTGTTGTTTTCGCCCCGCCCTGAATCTTCACCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCAc                                                                    <  1:485356/150‑1 (MQ=255)                                                                                  tCGTAACCCAGCACGTCGCCCATCAGCGGTGCTGTTTTCGGCCCGACCTAATTTTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGTGCGTGCACCAAAGAACTGCATTTGCTTACCAATCAcc                                                                   <  2:892748/150‑1 (MQ=255)                                                                                       aTCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTggg                                                             >  1:339792/1‑151 (MQ=255)                                                                                          cAGCCCGTCGTCCACCGGCGGGGTTGTTTCCCGCCGGCCCTGAATCTTCACCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTg                                                          <  1:404612/151‑1 (MQ=255)                                                                                          cAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGc                                                            >  1:558938/1‑149 (MQ=255)                                                                                          cAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTg                                                          >  2:947207/1‑151 (MQ=255)                                                                                             cACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACg                                                       >  1:781521/1‑151 (MQ=255)                                                                                               cgtcgtCCATCAGCGGTGCTGTTTTCGGCCCGCCCTAAATCTTCAGCTTCTCTTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgca                                                     <  2:164736/151‑1 (MQ=255)                                                                                                  cgtcCACCGGCGGCTTTGTTTTCGGCCCGCCCTGAATCTCCATCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagc                                                   <  1:897630/150‑1 (MQ=255)                                                                                                   gtcCATCGGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagc                                                   <  2:462447/149‑1 (MQ=255)                                                                                                     ccATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGc                                               >  2:351094/1‑151 (MQ=255)                                                                                                           gCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcg                                          <  2:751975/150‑1 (MQ=255)                                                                                                                  tttttgCGGCCCGACCTGAATCTTCAGCTTCTCCTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTa                                      <  1:895423/145‑1 (MQ=255)                                                                                                                    ttttCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgtc                                >  2:878026/1‑151 (MQ=255)                                                                                                                     tttCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgtcg                               >  1:916990/1‑151 (MQ=255)                                                                                                                      ttCGGCCCGCCCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgtc                                <  2:36642/149‑1 (MQ=255)                                                                                                                         ggCCCGCCCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCt                             <  2:669816/149‑1 (MQ=255)                                                                                                                                ctggAATCTCCACCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAg                      <  1:678094/146‑1 (MQ=255)                                                                                                                                  tgtaTCTTCCGCTCCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAg                      <  2:66519/144‑1 (MQ=255)                                                                                                                                  tGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGt                     >  2:692528/1‑148 (MQ=255)                                                                                                                                      tCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACg              >  2:103667/1‑151 (MQ=255)                                                                                                                                          cAGTTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACg              <  1:922456/147‑1 (MQ=255)                                                                                                                                           aGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc          >  1:707066/1‑150 (MQ=255)                                                                                                                                              ttCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCa         <  2:921547/148‑1 (MQ=255)                                                                                                                                               tctcGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc          >  2:87077/1‑146 (MQ=255)                                                                                                                                                ctcGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc          >  2:353166/1‑145 (MQ=255)                                                                                                                                                ctcGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGAt      <  2:535590/149‑1 (MQ=255)                                                                                                                                                  cttCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATc     <  2:422604/146‑1 (MQ=255)                                                                                                                                                  cGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGa       <  1:583998/146‑1 (MQ=255)                                                                                                                                                   gTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTc  >  2:28788/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                  ATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTC  >  NC_000913/3260905‑3261200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑