Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,258,974	0	T	G	42.0%	84.4 / 12.3	50	G411G (GGT→GGG)	dinF	oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein
Reads supporting (aligned to +/- strand):  ref base T (6/23);  new base G (21/0);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 5.11e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGG  >  NC_000913/4258836‑4259124                                                                                                                                           |                                                                                                                                                        gcagcTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCACAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCCGGGTTTGCGCTGGCg                                                                                                                                             >  1:957031/1‑151 (MQ=255)   agcTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCCGGGTTTGCGCTGACGc                                                                                                                                            >  2:385795/1‑150 (MQ=255)       ggctgACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCCCCGGGTTTGCCCCGACGCTCCtt                                                                                                                                       >  1:61779/1‑151 (MQ=255)          tgACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTTCGCTGACGCTCCtt                                                                                                                                       >  2:506052/1‑148 (MQ=255)           gACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTTCGCTGACGCTCCTTACg                                                                                                                                    >  2:500973/1‑150 (MQ=255)               gCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGcc                                                                                                                               <  1:117611/151‑1 (MQ=255)               gCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGcc                                                                                                                               <  2:327103/151‑1 (MQ=255)                  atcttatctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGcca                                                                                                                              <  1:628896/149‑2 (MQ=255)                     ttatctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGTGCTGACGCTCCTTACGCTGCCGTgg                                                                                                                           >  2:185648/1‑149 (MQ=255)                      tatctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCCCAGGGTTTGCGGTGACGCTCCTTACCCTGCCGTGGc                                                                                                                          >  2:13462/1‑149 (MQ=255)                       atctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCACAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCCCAGGGTTTTCGCCGACGCTCCTTACGCCGCCGTGGCTgg                                                                                                                       >  1:783086/1‑151 (MQ=255)                         ctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCCCCGGGTTTTCGCTGACGCCCCCTACGCCGCCGTGGCTggg                                                                                                                      >  1:435149/1‑150 (MQ=255)                             cAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCCCAGGTTTTGCGCTGACGCCCCTTACGCTGCcggggcgggg                                                                                                                      >  1:185356/1‑146 (MQ=255)                             cAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATc                                                                                                                 <  1:789614/151‑1 (MQ=255)                               ggTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCAt                                                                                                               <  1:319775/151‑1 (MQ=255)                                      ttGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGAGGCCGCCGCAGGGTTTGGGCTGGCGCTCCTTTCGCTGCCGGGGGTGGGGAATCaatgtt                                                                                                           >  1:762946/1‑143 (MQ=255)                                        gCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCCGGGTTTTCGCTGACGCTCCCTACGCTGCCGTGGCTGGGGAAATATGCCTTTtgg                                                                                                      >  1:387334/1‑151 (MQ=255)                                         ccGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGCTTTGtgg                                                                                                      >  1:743021/1‑150 (MQ=255)                                            gtggtTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCCGGGTTTTCGCTGACGCTCCCTACGCCGCCGTGGCTGGGGAATCATTCTTTGtgg‑ctgg                                                                                                 >  1:425712/1‑151 (MQ=255)                                            gtggtTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtgg‑ctgg                                                                                                 <  2:743545/151‑1 (MQ=255)                                                  ggCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTa                                                                                            <  1:205617/150‑1 (MQ=255)                                                       cTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCCGGGTTTTCGCCTACGCCCCTTAAGCTGCCGGGGCTGGGGAATCATGCTTTGTGGCCTGGCTTTAAcctc                                                                                       >  2:912498/1‑149 (MQ=255)                                                          gtgtTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCttt                                                                                   <  1:963982/151‑1 (MQ=255)                                                            gtTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCt                                                                                 <  2:805214/151‑1 (MQ=255)                                                              tATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTg                                                                                <  2:795245/150‑1 (MQ=255)                                                                  tgctgGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTTCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGCTTTTTGG‑CTGGCATTAACCGGCTTTCTGGCg                                                                             >  2:961142/1‑149 (MQ=255)                                                                  tgctgGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAAACATGCTTTGTGG‑CTGGCATTAAACGTCTTTCTGGGGtt                                                                           >  2:63448/1‑151 (MQ=255)                                                                   gctgGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGc                                                                              <  1:739173/147‑1 (MQ=255)                                                                   gctgGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGc                                                                              <  2:498514/147‑1 (MQ=255)                                                                     tgGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCCGGGTTTTCGCTGAAGCTCCTTTCGCCGGCCTGGGCGGGGAATCATGCTTTTTGG‑CTGGCGTTTACCGTCttttttggggt                                                                           >  1:377675/1‑140 (MQ=255)                                                                     tgGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTTCGCTGGCCCTCCTTTCGCCGCCGGGGCTGGGGAATCATGCTTTTTGG‑CTGGGCTTTAACGCCTTTTTTGGGTTgcg                                                                        >  1:809489/1‑151 (MQ=255)                                                                      ggACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTgc                                                                         <  1:941698/149‑1 (MQ=255)                                                                       gACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGcgcg                                                                      <  2:598934/151‑1 (MQ=255)                                                                        aCGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCCCCCCAGGTTTTGCGCTGACGCCCCTTACGCTGCCGTGGCTGGGGAATCATGCTTTTTGG‑CTGGCCTTTACCGTCCTTTTTGCGCTTCGCgg                                                                     >  1:784623/1‑151 (MQ=255)                                                                           gCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCt                                                                  <  1:716629/151‑1 (MQ=255)                                                                                   ataGGCGCAACGCGCGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTGCGCTGACGCTCCTTACGCTGCCGTGGGTGGGGAATCAAGCTTTGTGG‑CTGGCATTTAACGTCTTTTTGGGGGTTCGCggggctt                                                                >  2:63527/1‑141 (MQ=255)                                                                                           aaCGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATc                                                  <  2:176889/151‑1 (MQ=255)                                                                                                  gCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTggcggc                                           <  2:89372/151‑1 (MQ=255)                                                                                                     aCCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGCTTTGTGG‑CTGGCATTAAACGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTTGCGGCGTc                                        >  2:650221/1‑151 (MQ=255)                                                                                                      ccGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCa                                       <  1:818640/151‑1 (MQ=255)                                                                                                      ccGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGgcggcggc                                        >  2:652261/1‑148 (MQ=255)                                                                                                          aaTGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTGCGCTGACGCTCCTTAAGCCGCCGTGGCTGGGGAATCATGCTTTGTGG‑CTGGCATTTACCGTCTTTCTTGCGTTGCGCGGGGTTTCTCTGGCGGCTATCTGGCGGCGTCACTgg                                   >  1:123336/1‑151 (MQ=255)                                                                                                          aaTGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTGCGCTGACGCCCCTTACCCCGCCGTGGCTGGGGAATCATGCTTTTTGG‑CCGGCATTAAACGGCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGgcggcg                                          >  2:310631/1‑144 (MQ=255)                                                                                                           aTGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGgc                                  <  1:549282/151‑1 (MQ=255)                                                                                                                 aaCAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATg                            <  2:61109/151‑1 (MQ=255)                                                                                                                       aTGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTAcc                       <  2:803468/150‑1 (MQ=255)                                                                                                                          gCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTgg                    >  2:848018/1‑150 (MQ=255)                                                                                                                               ggCCGCCGCAGTTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTATCCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGc               <  2:212547/150‑1 (MQ=255)                                                                                                                                   gccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCa           >  2:257948/1‑150 (MQ=255)                                                                                                                                      gcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGt       <  2:467937/151‑1 (MQ=255)                                                                                                                                           ttttGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACgg  >  2:340270/1‑151 (MQ=255)                                                                                                                                           ttttGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACgg  >  1:324516/1‑151 (MQ=255)                                                                                                                                           |                                                                                                                                                        GCAGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGG‑CTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGG  >  NC_000913/4258836‑4259124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑