Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,337,723 | 0 | A | C | 43.6% | 88.2 / 18.1 | 56 | G566G (GGT→GGG) | gyrA | DNA gyrase (type II topoisomerase), subunit A |
Reads supporting (aligned to +/- strand): ref base A (27/4); new base C (0/24); total (27/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 9.40e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTAC > NC_000913/2337574‑2337868 | ccGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTAc > 2:657339/1‑151 (MQ=255) gcgcgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTACTACGTGCGGCAGATTTACCt > 2:696536/1‑151 (MQ=255) gcgcgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCt < 1:617108/151‑1 (MQ=255) gcgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCttt > 1:357552/1‑151 (MQ=255) cgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTc > 2:389756/1‑151 (MQ=255) cgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTc > 1:417510/1‑151 (MQ=255) gcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTcc > 2:679976/1‑151 (MQ=255) aCGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGcc > 2:874357/1‑150 (MQ=255) tGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCacgacg > 1:622098/1‑151 (MQ=255) ttCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTgc > 1:47000/1‑151 (MQ=255) ccGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTgcgc > 1:1114017/1‑151 (MQ=255) aaCTGATAAACTTTCATCGAATAGACGCGCCCCCGGCGGGAGAACCACAAAATATGGTCGTGGGTGTTCCCCCCCAGCAGTCGGTCGAAAAAGTCTTCTTCTTAAATACGTGCGGCAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGt < 1:359558/151‑1 (MQ=255) aaCTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATTTGTTCGTGGGTTTTCGCCCCCAGCAGTCGTTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTc < 1:52570/149‑1 (MQ=255) aatcTTTCATCGAATAGACGCGCCCACGGCTGGAGAAGCAAAGAATATGGTGGTGGGTGTTCCCCACCAGCAGTCGGTCAATAAAGTCTTCTTCTTTAATACGGGCGGCATTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGa < 1:953445/148‑1 (MQ=255) cTTTCATCGAATAGACGCGACCACGGCTGGCGAAGCACAGAATATGGTCGCGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGaa > 1:140734/1‑149 (MQ=255) tttCATCGAATAGACGCGCCCCCGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCCCCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAg < 1:241796/150‑1 (MQ=255) tttCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGc > 1:470896/1‑151 (MQ=255) ttCATCGAATAGACGCGCCCGCGGCGGGAAAAGCACAGAATATGTTCGGGAGTGTCCGCCACCGGCAGTCGGTCGAAAAAGTCTTCTTCTTAAATACGTGCGGAAAATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGc < 2:269826/150‑1 (MQ=255) agcGAATAGACGCGACCACGGCTGGAGAAGCAAAGAATATGGTGGTGATTGTCCCCCCCCAGCAGCCGGTCAATAAAGTCTTCTTTTTTAATACGTGGGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCt < 2:652923/149‑1 (MQ=255) tCGAATAGACGCGACCACGGCTGGCGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTg > 1:1125676/1‑151 (MQ=255) cGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTg > 2:135907/1‑150 (MQ=255) gAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGAt > 2:320369/1‑151 (MQ=255) aaTAGACGCGCCCGCGGCGGGAGAAGCAAAGAATATGGTGGTGTGTGTCCGCCCCCACCAGTCGGTCGATAAAGTCTTCTTTTTTAATACGGGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATa < 1:87644/151‑1 (MQ=255) agagccgcgCCCACGGCTGGAAAAGCAGAGAATATGGTCGTGAGTGTTCCCCCCCAGCAGTCGGCGAATAAAGTCTTCTTCTTAAATCCGTGCGGGATATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGTTg < 2:1012852/142‑1 (MQ=255) cgACCACGGCGGGAGAAGCACAAAATATGGTCGTGGGTGTTCCCCCCCAGCAGTCGGTCGATAAAGTCTTCTTTTTTAATCCGTGCGGAAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACt < 1:94075/145‑1 (MQ=255) cGGCTGGAGAAGCACAGAATATGGTCGTGAGTGCTCGCCACCAGCAGTCGGTCGCTAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAAccaagc > 2:948623/1‑143 (MQ=255) ggCTGGAGAAGCACAGAATAGGGTCGTGGGTGTTCGCCACCAGCAGTCGGTCGATAAAGTTTTCTTTTTTAATCCGTGCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTg < 1:421720/151‑1 (MQ=255) gggAGAAGCACAAAATATGGTCGTGAGTGTCCGCCACCAGCGGTCGGTCGATAAAGTCTTCTTTTTTAATGCGGGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTg < 2:1092854/150‑1 (MQ=255) aaaaGCAAAGAATATGGTGGTGAGTTTTCCCCACCAGCAGTGGGTCAAAAAATTTTTTTTCTTAAATCCGTGCGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgaga < 1:722709/149‑1 (MQ=255) gaAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgag > 2:265201/1‑149 (MQ=255) aaGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgag > 1:304285/1‑148 (MQ=255) accaaaaaaTATGGTCGTGAGTGTCCGCCCCCAGCAGTCGGTCGAAAAAGTCTTCTTCTTTAATACGTGGGGAAATTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGagagag < 2:357502/144‑1 (MQ=255) gCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGc > 2:864793/1‑151 (MQ=255) gggtgtCCGCCACCAGCGGTCGGTCGATAAAGTCTTCTTTTTTAAGCCGTGCGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTc < 2:867511/149‑1 (MQ=255) aGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTcc > 2:242012/1‑151 (MQ=255) ttCGCCCCCAGGGGTCGGTTAAAAAATTCTTTTTTTTTAATCCGTGCGGAAATTTTTCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCt < 2:597358/148‑1 (MQ=255) cagTCGGTCAATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGAta < 2:1055156/150‑2 (MQ=255) cggtcgATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTcc > 2:861381/1‑150 (MQ=255) ggtgataaacgccttgttcatAAATGCGTGCGGCAATTTCACCTCCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCa < 2:449009/138‑1 (MQ=255) ggtcgATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTcc > 2:516793/1‑149 (MQ=255) ggtcgAAAATGTTTTCTTCTTTAATTCGTGCGGGAGTTTTCCTTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATc < 2:554541/145‑1 (MQ=255) ggtcgAAAAAGTCTTCTTTTTTAATCGGGGCGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATc < 2:863214/145‑1 (MQ=255) ggtcgAAAAAGTCTTCTTCTTAAATACGTGCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCa < 1:367197/150‑1 (MQ=255) ggtcaAAAAATTCTTCTTCTTTAATCCGTGCGGAAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCt < 2:270244/146‑1 (MQ=255) ggtcaAAAAAGTCTTCTTCTTTAATCCGGGGGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCtt < 1:657428/147‑1 (MQ=255) gtcgATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAgg > 1:997000/1‑151 (MQ=255) tcgATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGt > 1:213895/1‑151 (MQ=255) gATAAAGTCTTCTTCTTTAATCCGTGCGGCAGTTTTCCCTTT‑CCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTg < 1:896132/150‑1 (MQ=255) tAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGAt > 2:907487/1‑151 (MQ=255) tttttaaaTCCGTGCGGAAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTgc < 2:847832/141‑1 (MQ=255) tcttTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGt > 2:1152392/1‑151 (MQ=255) gTGCGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGa < 1:389810/150‑1 (MQ=255) gcgggaaattttCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCgg < 2:762926/137‑1 (MQ=255) ggaagttttccctttcccGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTc < 1:696627/144‑1 (MQ=255) ggCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGAt < 1:179894/147‑1 (MQ=255) gATTTCCTTTTGCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTc < 1:612319/146‑1 (MQ=255) gATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTac > 1:1048485/1‑151 (MQ=255) | CCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTAC > NC_000913/2337574‑2337868 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |