Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,676,106	0	C	A	43.7%	103.3 / 36.0	71	L240F (TTG→TTT)	yphD	putative sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (29/11);  new base A (0/31);  total (29/42)
Fisher's exact test for biased strand distribution p-value = 5.86e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTG  >  NC_000913/2675960‑2676250                                                                                                                                                    |                                                                                                                                                 gTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACaaa                                                                                                                                               >  2:497053/1‑150 (MQ=255)  tAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAAt                                                                                                                                             >  1:286448/1‑151 (MQ=255)   aaTCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAAt                                                                                                                                             <  1:1015861/150‑1 (MQ=255)      cACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGcc                                                                                                                                          <  1:178582/150‑1 (MQ=255)        ccAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGt                                                                                                                                       <  1:14034/151‑1 (MQ=255)         cAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTc                                                                                                                                      <  2:1111237/151‑1 (MQ=255)                ccaaccaaTGTCCCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCAccgccg                                                                                                                               <  2:595363/151‑1 (MQ=255)                    gCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACAAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCta                                                                                                                            >  2:128974/1‑150 (MQ=255)                     cAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCtaat                                                                                                                          >  1:960126/1‑151 (MQ=255)                          tACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATcc                                                                                                                     >  1:313297/1‑151 (MQ=255)                            caaaggggcgAACCCCGCCCCCCGGAAAGTACCGACCCCCCGCCCACGGCCGGGGCGGGGACGTCAACCTCAAGCCCTTTGGCGGCCCCTGCGTTACCAGAACGGAGG‑CGCGCCGCCAAAAAAATGCCGGTCACCGCCGCta                                                                                                                            <  2:133291/133‑1 (MQ=255)                             caaaCAGGGACCCGCGCCCGCCGAAAAGTGCCGTACCGCCGACCACGCCCGCGGGGATGACGTCAAACTCCAGACCGTTTGCGGCCCCTGCGTTCCCAAAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATcc                                                                                                                     <  2:137731/146‑1 (MQ=255)                               aaCAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGaa                                                                                                                 >  1:1019055/1‑150 (MQ=255)                                   gggACCCGCGACCGCCGGAAAGTGCCGTACCGCCGCCCACGCCCGCGGCGATGGCGTCAACCTCCAGACTGTTTGCGGCACCTGCGTTCCCAGAACCGAGG‑CGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGa                                                                                                                  <  2:951015/145‑1 (MQ=255)                                      aaCCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTaa                                                                                                          >  2:150322/1‑150 (MQ=255)                                        ccGCGCCCGCCGGAAAGTGCCGTACCGCCGCCCACGACCGCGGCGATGACGTCAAACCCCAGCCCGTTTGCGGCACCTGCGTTCCCAGAACCGAGG‑CGCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTaaa                                                                                                         <  2:629311/149‑1 (MQ=255)                                         cgcgACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTaa                                                                                                          >  2:353402/1‑147 (MQ=255)                                          gcgACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGAt                                                                                                      >  1:761950/1‑150 (MQ=255)                                              ccgcggGAAAGTGCCGTCCCGCCGCCCACGCCCGCGGCGATGACGTCAAACCCCAGACCGTTTGCGGCCCCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAAAAAAATGCCGGTCGCCGCCGCTAATAATCCCGAAAGGGTAAAg                                                                                                        <  2:701608/144‑1 (MQ=255)                                              ccgccgGAAAGTGCGGTCCCGCCGACCCCGCCCGCGGCGATGACGTAAAACCCCAGCCCTTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGa                                                                                                  <  1:820571/150‑1 (MQ=255)                                               cgccgAAAAGTGCCGTCCCGCCGCCCACGCCCGCGGCGATGACGTCAACCTCCAGACCGTTGGCGGCACCTGCGTTCCCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAAt                                                                                                <  1:568082/151‑1 (MQ=255)                                               cgccgAAAAGTGCCGTACCGCCGACCCCCCCCGCGGCGATGACTTCAAACCCCAGACCGTTGGCGGCACCTGCGTTCCCAGAACCGAGG‑CGCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGAt                                                                                                      <  2:597119/145‑1 (MQ=255)                                                gccgGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATg                                                                                               >  2:171993/1‑151 (MQ=255)                                                 ccgAAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTAAAACTCCAGCCCGTTGGCGGCCCCTGCTTTCCCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATg                                                                                               <  1:206408/150‑1 (MQ=255)                                                  cgGAAAGTGCCGTACCGCCGACAACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcg                                                                                             >  2:1095475/1‑151 (MQ=255)                                                   gaaaaGTCCCGTCCCGCCGACCACGACCGCGGCGATGACGTCAAACCCCAGACCGTTGGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcg                                                                                             <  2:1162727/148‑1 (MQ=255)                                                     aaaGTGCCGTCCCGCCGCCCACGCCCGCGGCGATGACGTCAAACTCCAGACCTTTGGCGGCCCCTGCGTTACCAGAACCGAGG‑CGCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCa                                                                                           <  2:668388/150‑1 (MQ=255)                                                        gcgccgccccgccgCCCCCGCCCGCGGCGATGACGCCAAACCCCAGACCGTTTGGGGCCCCTGCGTTACCAGAACCGAGG‑CGCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcg                                                                                       <  2:99197/143‑1 (MQ=255)                                                        gTGCCGTCCCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGCCACCGGCGTTACCAGAACCGAGG‑CGCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCAccc                                                                                        <  2:737333/150‑1 (MQ=255)                                                           ccctccccccgACCCCGCCCGGGGCGATGACGTAAAACCCCAGACCTTTGGCGGCCCCTGCGTTACCAAAACCGAGG‑CGCGCCGCAAAAAAAATGCCGTTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcgac                                                                                     <  2:810256/142‑1 (MQ=255)                                                           ccGTCCCGCCGCCCACGACCGCGGCGATGACGTCAAACCCCAGCCCGTTTGCGGCACCTGCGTTCCCAGACCCGAGG‑CGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcg                                                                                       <  1:51292/148‑1 (MQ=255)                                                            cGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcgacg                                                                                    >  1:690309/1‑150 (MQ=255)                                                                ccgccgACCACGACCGGGGCGATGACGTCAACCCCCAGCCCGTTTGCGGCCCCGGCGTTACCAAAACCGAGG‑CGCGCCGCAAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAAc                                                                                 <  2:489455/149‑1 (MQ=255)                                                                ccgccgACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGt                                                                               >  1:182413/1‑151 (MQ=255)                                                                   ccgCCCACGCCCGCGGCGATGACGTCAAACCCCAGACCGTTTGCGGCACCTGCGTTCCCAGAACCGAGG‑CGCGCCGCAAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTg                                                                             <  2:48687/150‑1 (MQ=255)                                                                   ccgCCCACGCCCGCGGCGAGGACGTCAAACTCCAGCCCGTTGGCGCCCCGTGCGTCCCCAGAACCGAGG‑GGCGCCGCCAAAAAAATGCCGGCCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGtt                                                                              <  2:1158011/149‑1 (MQ=255)                                                                   ccgACCACGACCGCGGGGATGACGTCAAACCCCAGCCCGTTTGCGGCACCTGCTTTCCCAGACCCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTg                                                                             <  1:952950/150‑1 (MQ=255)                                                                    cgACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGAt                                                                           >  2:63891/1‑151 (MQ=255)                                                                        cACGCCCGCGGGGATGACGTAAAACCCAAGACCGTTTGCGCCACCGGCGTTCCCAGAACCGAGG‑CGCGCCGCCAAAAAAACGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCg                                                                       <  2:141153/151‑1 (MQ=255)                                                                        cACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGcc                                                                        >  2:93562/1‑150 (MQ=255)                                                                          cGACCGCGGCGATAACGTCAAACCCCAGACCTTTGGCGCCCCCTGCTTTCCCAAAACCAAGG‑CGGGCGTCCAACAAAATGCCGATCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCGCCCGACGAACGTTGATGCCGc                                                                      <  1:409761/150‑1 (MQ=255)                                                                                 ggcgAAGACTTCAACCTCCAGACCGTTTGCGGCACCTGCTTTCCCAGACCCGAGG‑CGCGCCCCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACt                                                                <  2:509007/149‑1 (MQ=255)                                                                                       gACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCgccgcc                                                        >  1:315125/1‑151 (MQ=255)                                                                                          gTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGccgccg                                                       >  2:1085864/1‑149 (MQ=255)                                                                                           tCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGt                                                      >  2:948911/1‑149 (MQ=255)                                                                                           tCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGt                                                      >  2:356298/1‑149 (MQ=255)                                                                                                cTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCAtt                                                >  2:450167/1‑150 (MQ=255)                                                                                                 cccAGCCCGTTTGCGGCACCTGCGTTCCCAGACCCGAGG‑CGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTAc                                              <  1:440190/150‑1 (MQ=255)                                                                                                     gACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGCCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGcc                                          >  1:488516/1‑151 (MQ=255)                                                                                                          tttGCGGCCCGTGGTTCACAAAAACCGGGG‑CCCCCCCCAAAAAAAAGGCCGGCCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAAc                                       <  2:492649/149‑1 (MQ=255)                                                                                                                 ccccTGCGTTCCCAGAACGGGGG‑CGCACCGCCAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCa                                   <  2:162608/144‑1 (MQ=255)                                                                                                                      gCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAgc                         >  1:726637/1‑151 (MQ=255)                                                                                                                       ctttcccaaacccGGGG‑CCCCCCGCAAAAAAACTCCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCg                            <  2:881954/136‑1 (MQ=255)                                                                                                                        gTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAgcgc                       >  2:428034/1‑151 (MQ=255)                                                                                                                         ttcccAGACCCGAGG‑CGCGCCGCAAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGa                           <  1:893560/143‑1 (MQ=255)                                                                                                                          tcacaaaaCCGGGGCCGCGCCGCAAAAAAAAGGCGGGTAACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAg                          <  1:252139/141‑1 (MQ=255)                                                                                                                             caaaaCCGGGG‑CCCCCCGCCAAAAAAAGGCGGCTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAgcgc                       <  2:286769/143‑1 (MQ=255)                                                                                                                                acccGGGG‑CGCCCCGCCAAAAAAAGGCCGGTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAg                 <  2:1018930/147‑1 (MQ=255)                                                                                                                                acccGGGG‑CCCCCCCCCAAAAAAAGGCCGTTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAgg                <  2:905740/148‑1 (MQ=255)                                                                                                                                 aCCGAGG‑CGCGCCCCCCACAAAATCCCGGTCACCGCCGCTAATAATCCCGAAAGGGCAAAGCTAAGAATGCGCACCCGACGAACGTTGATGCCGCACCACCGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGc               >  2:324630/1‑150 (MQ=255)                                                                                                                                  ccGAGG‑CGCGCCGCAAAAAAAAGGCCGTTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCg              <  2:1131420/150‑1 (MQ=255)                                                                                                                                  ccGAGG‑CGCCCCGCCAAAAAAAGCCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCgg             <  2:1054244/151‑1 (MQ=255)                                                                                                                                   cGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGt            >  2:889204/1‑151 (MQ=255)                                                                                                                                     aGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGttt          >  2:833075/1‑151 (MQ=255)                                                                                                                                      gg‑CGCGCCCCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGttt          <  1:275489/150‑1 (MQ=255)                                                                                                                                      gg‑CGCCCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGtt           <  1:585230/149‑1 (MQ=255)                                                                                                                                         cgcgcCCCAAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGttt          <  1:221225/148‑1 (MQ=255)                                                                                                                                           cgccgcCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCgg     >  1:1132932/1‑151 (MQ=255)                                                                                                                                             ccgccAAAAAAATGCCGGCCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCt   <  2:101262/151‑1 (MQ=255)                                                                                                                                              cgccAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCtg  >  2:901429/1‑151 (MQ=255)                                                                                                                                               gccAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCtg  >  2:60561/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTG  >  NC_000913/2675960‑2676250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑