Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913589,9300AG41.7% 46.9 / 11.6 36L342P (CTC→CCC) nfrAbacteriophage N4 receptor, outer membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (4/17);  new base G (15/0);  total (19/17)
Fisher's exact test for biased strand distribution p-value = 7.21e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAACA  >  NC_000913/589784‑590080
                                                                                                                                                  |                                                                                                                                                      
gCATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAggg                                                                                                                                                     <  2:372644/150‑1 (MQ=255)
 cATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTg                                                                                                                                                   <  1:331850/151‑1 (MQ=255)
    cAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAAATTTGCCCGTTCTTTCTGATACAAGAAACGTGCCAGACGCAGCGCTTCAGGCTTTTTTCGGGTCTCCACGCTGGCAGCCTAACGCCCCCCAAACATTTCATTgggggggggggggggg                                                                                                                                                >  2:1587/1‑149 (MQ=255)
     aGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTTCTGATACAGCAATCGTGCCAGGCGCCGAGCTTCAGGCCTGTTTCGGGTCGCCACGCTGACAGCATAACGCCCCCCAAGCCTTTTATTGGCCggggggggggggg                                                                                                                                               >  2:570593/1‑148 (MQ=255)
     aGTTGCCAGGTTAGTTGATCCAGGCGGGGAAGATTTGCCGGGTTTTGCTGATAAAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCCCGCTGACAGCATAACGCTCCTCAAGCCTTTCATTGGCGggggggggggggg                                                                                                                                               >  2:163111/1‑148 (MQ=255)
         gCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAg                                                                                                                                           <  1:102313/151‑1 (MQ=255)
         gCCAGGTTAGTTGATCCAGGCGGGGAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCCTCAGCCTTGTTTCGGGTCGCCACGCTGACAGCCTAACGCTCCTCAAGCATTTCATTGGCGgggggggggggg                                                                                                                                                >  2:1117859/1‑144 (MQ=255)
          ccAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGGCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGgggggggggggggggcgg                                                                                                                                          >  1:646565/1‑143 (MQ=255)
                 aGTTGATCCAGGCGGGTAAGATTTGCCGGGTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCCCCTCCAGCATTTCATTGGCGgggggggggggggggggggtttt                                                                                                                                     >  2:480245/1‑136 (MQ=255)
                        ccAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGcgtc                                                                                                                            <  1:584888/151‑1 (MQ=255)
                        ccAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGcgtc                                                                                                                            <  1:65111/151‑1 (MQ=255)
                             cGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGTGGCGAGGAGGTTTTGCGCTTCCCCGTACt                                                                                                                       >  1:4372/1‑151 (MQ=255)
                                  aaGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACct                                                                                                                  <  1:317257/151‑1 (MQ=255)
                                   aGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACctt                                                                                                                 <  2:283024/151‑1 (MQ=255)
                                     aTTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACcttct                                                                                                               <  2:442399/151‑1 (MQ=255)
                                         gCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAGCAGTTTTTGCGCGGCGTCGTAATGACCTtttttt                                                                                                            >  1:278163/1‑150 (MQ=255)
                                         gCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTa                                                                                                           <  1:780500/151‑1 (MQ=255)
                                          ccGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGGGGAGTTTTTTTGCTGCGTCGTACTGACCTTCtttt                                                                                                            >  1:7449/1‑149 (MQ=255)
                                           cGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGCGGTTTTTTGCCTCGTCGTTCTTGCCTTCtttt                                                                                                            >  2:21082/1‑148 (MQ=255)
                                           cGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAGCAGTTTTTTCGCTGGGTCGTACTGACCTTCttttta                                                                                                          >  1:163583/1‑148 (MQ=255)
                                               tCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTCGCTGCGGCGTACTGACCTTCTTTTTACaac                                                                                                      >  1:405013/1‑148 (MQ=255)
                                                cTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAGCAGGTTTTGCGCTGCGGCGTACTGACCTTCTTTTTAAAGCAc                                                                                                    >  1:75506/1‑151 (MQ=255)
                                                   gCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCgg                                                                                                 <  2:440767/151‑1 (MQ=255)
                                                     tGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGGGGGGGGCAGGTTTTTCGCCGCGTCGGGCTGACCTTCTTTTTACAGCAcc                                                                                                   >  2:647721/1‑147 (MQ=255)
                                                           aGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTcg                                                                                         <  1:643895/151‑1 (MQ=255)
                                                             cAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTcgcg                                                                                       <  1:712343/151‑1 (MQ=255)
                                                              aaTCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAGCCGGTTTTGCGCCTCGTCGTCCCGACCTTCTTTTTAAAAGACCGGCAGCGTcgccc                                                                                      >  2:513412/1‑149 (MQ=255)
                                                                   tGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGGGCAGGTTTTGCGCTGCGTCGCACTGACCCTCTTTTTAACGCACCGGGAGGGGCGCGCccac                                                                                  >  2:940029/1‑150 (MQ=255)
                                                                       aGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAGCAGGTTTTTCGCTGCGTCGGACTGACCTTCTTTTTACAACAACCGTAGCGTCGCGCCCacaaca                                                                              >  1:957103/1‑150 (MQ=255)
                                                                          cGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACt                                                                          <  1:375985/151‑1 (MQ=255)
                                                                           gCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTTCGCTGCGTCGGTCTGAACCTCTTTTTATAGCACCGGGAGCGGCCCGCccacaa                                                                                >  2:29824/1‑144 (MQ=255)
                                                                                 cTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGTGGGGAGCCGTTTTTTCGCTGCGTCGTACTGACCTTCTTTTTAAAACACCGGGAGCGGCGCGCCCACAACATACTGGCggg                                                                    >  1:1019459/1‑149 (MQ=255)
                                                                                  ttCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGAGGAGTTTTTGCGCTGCGGCGTACTGAACTTCTTTTTTCAACACCGGTAGCGTCGCGCCCACAACAAACTGGccggt                                                                   >  1:718094/1‑146 (MQ=255)
                                                                                   tCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAGCAGGTTTTTCGCTTCGTCGTACTGAACTTCTTTTTACAAGAACGGGAGGGGCGCCCCCACAACAAAATGGCggggtt                                                                 >  2:469724/1‑147 (MQ=255)
                                                                                         ttGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGGGGCGAGCAGGTTTTTCGCTGCGTCCTACTGACCCTTTTTTTACAGCACCCGGAGCGTCGCGCCCACAAAATACTGGCgggttgtggg                                                             >  1:472476/1‑141 (MQ=255)
                                                                                                     gCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAGCAGTTTTTTCGCTGCGTCGTACTGACCTTCTTTTTAAAGCACCGGGAGCGTCGCGCCAACAACAAACTGGCGGGTGTTggggca                                                          >  2:1067949/1‑136 (MQ=255)
                                                                                                                   aTAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGcc                                 <  1:796705/151‑1 (MQ=255)
                                                                                                                     aaCGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTg                               <  1:17300/151‑1 (MQ=255)
                                                                                                                           cctcAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACg                           <  1:495234/149‑1 (MQ=255)
                                                                                                                                           ggCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTAttt          <  1:1169696/150‑1 (MQ=255)
                                                                                                                                              ggggAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTTGCCCACGCCTGAACCGGGGTGGCGCTGTAttt          >  1:608163/1‑147 (MQ=255)
                                                                                                                                                ggAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTTGGTAAAATGTACCCTATAATTTCCCCAAGCCCGCAAGGGGGTAGGGCGGTAttttt        >  2:330563/1‑145 (MQ=255)
                                                                                                                                                  aGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAACa  <  1:581092/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
GCATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAACA  >  NC_000913/589784‑590080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑