Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,851,044	T→G	N17H (AAC→CAC)	ilvN ←	acetolactate synthase 1 small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,851,044	0	T	G	100.0%	239.4 / NA	65	N17H (AAC→CAC)	ilvN	acetolactate synthase 1 small subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (32/33);  total (32/33)

ATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGCACATGGATCAGCGCCGG  >  NC_000913/3850899‑3851188                                                                                                                                                  |                                                                                                                                                 atcTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGa                                                                                                                                              >  1:895092/1‑150 (MQ=255)     gCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAAc                                                                                                                                            <  1:483492/148‑1 (MQ=255)     gCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACgg                                                                                                                                          <  2:817437/150‑1 (MQ=255)     gCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGt                                                                                                                                         <  2:335352/151‑1 (MQ=255)       tCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGGCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGGAAAAAGGCCACAAACGTGGGGCCTTAAGCCCGGATGGGGGCGAACGGt                                                                                                                                         >  2:616551/1‑149 (MQ=255)        ccAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTg                                                                                                                                        >  2:1061795/1‑149 (MQ=255)        ccAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGa                                                                                                                                       <  1:781506/150‑1 (MQ=255)                tGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGa                                                                                                                               >  1:385507/1‑150 (MQ=255)                 ggTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAAt                                                                                                                             >  1:619137/1‑151 (MQ=255)                       cATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGtt                                                                                                                       >  2:662550/1‑151 (MQ=255)                       cATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGtt                                                                                                                       <  2:1127169/151‑1 (MQ=255)                       cATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGtt                                                                                                                       >  2:889430/1‑151 (MQ=255)                         ttGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGGGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACgct                                                                                                                       >  2:328005/1‑147 (MQ=255)                              cAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATg                                                                                                                 >  1:553634/1‑150 (MQ=255)                                      aGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGtt                                                                                                        >  2:1105190/1‑151 (MQ=255)                                      aGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGtt                                                                                                        >  1:539468/1‑151 (MQ=255)                                      aGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGtt                                                                                                        <  2:149408/151‑1 (MQ=255)                                        atatGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGtttt                                                                                                      >  2:967108/1‑151 (MQ=255)                                            ggCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCa                                                                                                   <  1:1154382/150‑1 (MQ=255)                                                tttGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGc                                                                                               <  1:590014/150‑1 (MQ=255)                                                  tGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCtt                                                                                             <  1:545374/150‑1 (MQ=255)                                                  tGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCtt                                                                                             <  1:570971/150‑1 (MQ=255)                                                   gTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTAt                                                                                           >  2:70160/1‑151 (MQ=255)                                                     cGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTAtt                                                                                          >  2:998242/1‑150 (MQ=255)                                                     cGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTc                                                                                         <  2:451740/151‑1 (MQ=255)                                                       cTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTccc                                                                                       >  1:1042539/1‑151 (MQ=255)                                                       cTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCACGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTccc                                                                                       >  2:296621/1‑151 (MQ=255)                                                         gTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTcccc                                                                                      <  2:1773/150‑1 (MQ=255)                                                          tCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTccccc                                                                                     >  1:543591/1‑150 (MQ=255)                                                              gAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCccacca                                                                                 <  1:718018/150‑1 (MQ=255)                                                               aaTCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCAtt                                                                               <  1:165234/151‑1 (MQ=255)                                                                aTCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCAttt                                                                              <  2:474329/151‑1 (MQ=255)                                                                    gCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGt                                                                          <  1:921205/151‑1 (MQ=255)                                                                      aGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTAt                                                                        <  1:885648/151‑1 (MQ=255)                                                                       gACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTAtt                                                                       <  1:1053558/151‑1 (MQ=255)                                                                           aaGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTcgcc                                                                   <  1:817063/151‑1 (MQ=255)                                                                           aaGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTcgcc                                                                   >  2:829810/1‑151 (MQ=255)                                                                            aGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTcgccg                                                                  <  1:1106409/151‑1 (MQ=255)                                                                                tGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCCCAAACGTGGGGCATTACGACCGGAAGGCGGCGAACGGTGAGCTCCAGACTTACGTTGTCATGAGTTGTGTTTTGCCTTGCTTATTCCCCCACCATTTCAGGATTAGCCGCAcc                                                              >  2:256532/1‑151 (MQ=255)                                                                                   cTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTgg                                                           >  2:204731/1‑151 (MQ=255)                                                                                       aaCGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTggcggc                                                       <  2:1098845/151‑1 (MQ=255)                                                                                       aaCGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTggcggc                                                       >  1:472220/1‑151 (MQ=255)                                                                                           ttAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCAcc                                                    >  1:390640/1‑150 (MQ=255)                                                                                           ttAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGGGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACAATTTCAGTATTCGCCGAACCTGGCGGCAcc                                                    >  2:927124/1‑150 (MQ=255)                                                                                            tAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCAt                                                  >  1:270402/1‑151 (MQ=255)                                                                                             aaaaGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATc                                                 <  2:630021/151‑1 (MQ=255)                                                                                              aaaGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCg                                                >  1:391062/1‑151 (MQ=255)                                                                                                  cgcgGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTa                                            <  2:519958/151‑1 (MQ=255)                                                                                                     ggcggGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAAc                                         <  1:761905/151‑1 (MQ=255)                                                                                                         ggCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACtttt                                     <  1:1046557/151‑1 (MQ=255)                                                                                                          gCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACttttt                                    >  1:894182/1‑151 (MQ=255)                                                                                                           cAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACttttt                                    >  1:27328/1‑150 (MQ=255)                                                                                                             aaaaGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTttctt                                 >  1:263741/1‑151 (MQ=255)                                                                                                               aaGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCg                               <  2:533027/151‑1 (MQ=255)                                                                                                                aGGCCACAAACGTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCgg                              >  1:520526/1‑151 (MQ=255)                                                                                                                           gTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATgcgc                   >  1:535922/1‑151 (MQ=255)                                                                                                                           gTGGGTCATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATgcgc                   <  1:967227/151‑1 (MQ=255)                                                                                                                                 cATTACGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGCACATgg             <  1:579233/151‑1 (MQ=255)                                                                                                                                     aCGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGCACATGGATc          <  2:913739/150‑1 (MQ=255)                                                                                                                                      cGCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGCACATGGATc          >  2:159369/1‑149 (MQ=255)                                                                                                                                       gCCCGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGCACATGGATCAgc       <  2:154600/151‑1 (MQ=255)                                                                                                                                        cccGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGCACATGGATCAgcg      <  2:619052/151‑1 (MQ=255)                                                                                                                                         ccGGATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGCGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGGAAACTTTTTCTTCGGCATCAATGCGCACATGGATCAgc       >  2:624382/1‑149 (MQ=255)                                                                                                                                            gATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGCACATGGATCAGCGCCgg  <  2:472154/151‑1 (MQ=255)                                                                                                                                            gATGGTGGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGCACATGGATCAGCGCCgg  <  1:893262/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                 ATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGCACATGGATCAGCGCCGG  >  NC_000913/3850899‑3851188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑