Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,871	0	T	A	50.0%	49.7 / 32.5	50	G58G (GGT→GGA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (22/3);  new base A (0/25);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 5.18e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGT  >  NC_000913/1755724‑1756020                                                                                                                                                    |                                                                                                                                                      cATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATTGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTaaa                                                                                                                                                    <  1:154532/151‑1 (MQ=255)    tggACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAcc                                                                                                                                                 >  1:685986/2‑151 (MQ=255)    cGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAcc                                                                                                                                                 <  1:955572/151‑1 (MQ=255)     ggACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAccg                                                                                                                                                >  2:574579/1‑151 (MQ=255)      gACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAccgc                                                                                                                                               <  2:48968/151‑1 (MQ=255)                       gAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGAt                                                                                                                               >  2:905139/1‑150 (MQ=255)                          gagaTGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGGCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATAcc                                                                                                                            >  2:383412/1‑150 (MQ=255)                               gTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGt                                                                                                                      >  1:55460/1‑151 (MQ=255)                                 tAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTcc                                                                                                                    >  1:270647/1‑151 (MQ=255)                                  aGCAAAAATGCTGGCCGCTGGAATGAAGTTTAGGCGTCTGACCTCCTCTCATGGTGCCTAGGCAAAACCGGGTCAGCGCTTCCAGATTCTGCGCAAGGTGAGGAGAAAAACTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTc                                                                                                                     <  2:501784/149‑1 (MQ=255)                                   gcaaaaaTGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAGGGTGACTATGAAAAACACGGTCAGCGCATTAAGAATCTGCGAAACGTGATGAGAAAAACTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTc                                                                                                                     <  2:187930/145‑1 (MQ=255)                                      aaaaTGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGaa                                                                                                                >  1:515560/1‑150 (MQ=255)                                      aaaaTGCGGGCCGCTGAAATGAACGTTATGCGTCTAACCTTCTCTCGTGGTGACTAGACAAAACACGTTCAGCCCATTCAGATTCTGCGAAACGTGATGAGAAAAACTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGa                                                                                                                 <  1:846712/149‑1 (MQ=255)                                       aaaTGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAAt                                                                                                              >  1:152897/1‑151 (MQ=255)                                          tGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCg                                                                                                           >  1:502213/1‑151 (MQ=255)                                             tGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCacac                                                                                                        >  2:969163/1‑151 (MQ=255)                                              ggACGCTGGCATGACCGTTATGCGTCTAACCTTCTCTCATGGTGACTAGGCAAAACCCGGTCAGCTCATTCAGAATCTGCGCAACGTGATGAGAAAAATTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCAc                                                                                                        <  2:499863/150‑1 (MQ=255)                                              ggACGCGGGCATGATCGTTATGCGTCTAACCTTCTCTCGTGGTGACTAGGCAGAACCCGGTCGGCGCATTCAGATTCGGCGCAAGGTGAGGAGAAAACCGGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCg                                                                                                           <  2:570975/147‑1 (MQ=255)                                                  gCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATg                                                                                                    >  2:466685/1‑150 (MQ=255)                                                     ggaaTGAACGTTATGCGTCTAACCTTCTCTCAGGGTGACTATGCAGAACACGGTCAGCGCATTCAAAATCTGCGAAAGGTGATGAGAAAAACTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAAc                                                                                                <  2:813939/148‑1 (MQ=255)                                                     ggCATGAACGTTATGCGTCTGACCTCCCCTAAGGGTGAATAGGAAAAACCCGGCCAGCGCATTCAAAATCGGCGAAACGTGAGGAAAAAAACTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATg                                                                                                    <  1:785957/147‑1 (MQ=255)                                                         tGAACTTTATGCGTCTGACCTTCTCTCATGGTGACTATGAAAAACGCGGTCAGCGCTTTCAAAATCGGCGAAACGTGATGAGAAAAACTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACt                                                                                               <  1:764520/148‑1 (MQ=255)                                                         tGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTgg                                                                                             >  2:278225/1‑150 (MQ=255)                                                           aaCTTTATGCTTCTGAATTTCTCTCAGGGTGACTAGGCAAAACACGGTCAGCCCATTCAAAATCGGCGAAACGGGATAAAAAAAACGGAAAAACCCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAg                                                                                          <  1:302437/151‑1 (MQ=255)                                                            aCGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAgg                                                                                         >  1:722421/1‑151 (MQ=255)                                                            aCGTTATGCGTCGGATCTCCTCTCAGGGTAACTAGGAAAAACCCGGTCAGCCTATTCAAAATCTCCGAAACGGGATGAGCAAAATTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAgg                                                                                         <  1:895924/151‑1 (MQ=255)                                                                        taaccTTCCCTCAGGGTAACTAGACAGACCCGGGCCAGCCCATAAAAAATCGCCGAAACGTGATGAGAAAAATTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAacga                                                                                <  1:859132/144‑1 (MQ=255)                                                                         gAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGtt                                                                            >  2:49224/1‑151 (MQ=255)                                                                            cTTCTCTCATGGTGCCTATGAAAAACGCGGCCCGCCCTTTAAAATTCTGCACAACGTGATGAGAAAAACTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAacgacg                                                                              <  2:384532/146‑1 (MQ=255)                                                                             ttCTCTAAGGGTGACTATGAAAACCCCGGCCAGCGCATTAAGAATCTGCGAAACGGGAGGAGAAAAACTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTtc                                                                          <  2:462046/149‑1 (MQ=255)                                                                               ctctcATGGTGACTATGCAGAACACGGTCAGCGCATTCAAAATCTGCGCAACGTGATGAGCAAAACTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAacg                                                                                 <  2:314573/140‑1 (MQ=255)                                                                               ctctcATGGTGACTATGCAAAACACGGTCAGCCCATTCAGAATCTGCGAAACGTGATGAAAAAAACTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTtctc                                                                        <  2:622444/149‑1 (MQ=255)                                                                                 ctcATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGa                                                                     >  1:956174/1‑150 (MQ=255)                                                                                      ggTGACTATGAAAACCACGGACAGCTTATTAAAACCCGGCGAAGGGTGATGAAAAAACCTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAg                                                                  <  1:125726/148‑1 (MQ=255)                                                                                      ggTAACTATGCAGAACCGGGCCAGCCCATAAAGAATCTGCGAAACGTGATGAGAAAACCTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGc                                                                 <  1:866991/149‑1 (MQ=255)                                                                                      ggTAACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAAGGTGATGAGAAAAACTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGaaa                                                                   <  2:252217/147‑1 (MQ=255)                                                                                        tGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGt                                                             >  1:960724/1‑151 (MQ=255)                                                                                              tGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCACGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGAcc                                                       >  1:655969/1‑151 (MQ=255)                                                                                                    acacGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACt                                                 >  1:832372/1‑151 (MQ=255)                                                                                                                       aatcgccgcAACGTGATGAGCAAAACGGTAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGa                                      <  1:7061/137‑1 (MQ=255)                                                                                                                       aaTCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTg                              >  1:684931/1‑151 (MQ=255)                                                                                                                       aaTCGGCGCAACGGGATGAGAAAAGCGGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTg                              <  1:864404/151‑1 (MQ=255)                                                                                                                       aaTCGGCGAAACGTGATGAAAAAAACTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCt                               <  1:371495/150‑1 (MQ=255)                                                                                                                                agcgtaaggaaaaaaaCTGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCgg                       <  2:404304/137‑1 (MQ=255)                                                                                                                                aaggTGATGAAAAAAACTGAAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTAt                          <  1:268650/143‑1 (MQ=255)                                                                                                                                      ataaaaaaaaCGGGAAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCaa                    <  2:502229/140‑1 (MQ=255)                                                                                                                                         aaaaaaaCGGGAAAATCCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGaa             <  2:686009/147‑1 (MQ=255)                                                                                                                                           cAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATg          >  2:646440/1‑151 (MQ=255)                                                                                                                                             aaaCTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGt        >  1:938628/1‑151 (MQ=255)                                                                                                                                                   gTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGt  >  1:718534/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                      CATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGT  >  NC_000913/1755724‑1756020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑