Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,177,7480CA40.8% 76.2 / 24.3 49E55* (GAG→TAG) nudFADP‑ribose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (23/6);  new base A (0/20);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.63e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGT  >  NC_000913/3177606‑3177894
                                                                                                                                              |                                                                                                                                                  
cccTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAAtt                                                                                                                                             <  1:359637/150‑1 (MQ=255)
 ccTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTc                                                                                                                                           >  1:279656/1‑151 (MQ=255)
   tctTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTccc                                                                                                                                         >  1:96860/1‑151 (MQ=255)
    ctTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTcc                                                                                                                                          <  2:520663/149‑1 (MQ=255)
      tCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCccgcc                                                                                                                                      >  1:797798/1‑151 (MQ=255)
      tCAATCATCACGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTccc                                                                                                                                         <  1:60475/148‑1 (MQ=255)
           catcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCAc                                                                                                                                  <  1:857833/150‑1 (MQ=255)
             tcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTc                                                                                                                               >  1:931967/1‑151 (MQ=255)
                    aaCCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTc                                                                                                                        >  2:417896/1‑151 (MQ=255)
                     aCCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTc                                                                                                                        >  2:220648/1‑150 (MQ=255)
                          ctcCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGGGGAAACCCGAATCTGCCCAATCAGCACACCTTCATCCCGCCGTGGGCAAAGGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTg                                                                                                                   <  1:608734/150‑1 (MQ=255)
                              aGTAGCCAAGGGTTTTCGCGGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACACCTTCATCACGCACTGGGTAAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGt                                                                                                              <  2:450281/151‑1 (MQ=255)
                              aGTAGCCAAGGGGTATCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGt                                                                                                              >  2:540041/1‑151 (MQ=255)
                                     aaGGGGTTTCGCTGGTGTCGTACGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCACCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATa                                                                                                        <  1:327413/150‑1 (MQ=255)
                                       gggtTTCCGCTGTTGTCGTGCGGGGCAATCCGAATCTGCTCAATCACCACAACTTCACCCCCCCCGGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGa                                                                                                      <  1:36454/150‑1 (MQ=255)
                                       gggtTTCCGCTGGTGTCTTACGCGGCAATCCGAATCTCCTCAATCACAACACCTTCATCACGCTCTGGTTCAAAGGTTAGCAAGCCTGCGGGGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGAc                                                                                                     <  2:258733/151‑1 (MQ=255)
                                       ggggTTCAGCTGGTTCCGTCGGCGGAAATCCGAAGCTACCAAAACAGCCCAACTTCACCCCGCACTGGGAAAAGGGGCAGAAAAGGCGTGGTGAAACCGCGTAAAAAAATTGCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATa                                                                                                        <  1:591353/148‑1 (MQ=255)
                                        ggttttCGCTGGTGTCGTACGCGGCAATCCAAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTAAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACg                                                                                                    <  2:395726/148‑1 (MQ=255)
                                        gggTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCACGCACGGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTCAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACg                                                                                                    <  1:395926/151‑1 (MQ=255)
                                            ttCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg                                                                                                 >  2:280234/1‑150 (MQ=255)
                                                  ggTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAAt                                                                                          >  1:404240/1‑151 (MQ=255)
                                                     gtCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCt                                                                                        >  2:167745/1‑150 (MQ=255)
                                                       cGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtat                                                                                      <  2:260398/150‑1 (MQ=255)
                                                           cgcgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACGGGGTAAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAg                                                                                  <  2:631444/150‑1 (MQ=255)
                                                              ggCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCt                                                                              >  2:831722/1‑151 (MQ=255)
                                                                 aaTCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAAt                                                                           >  2:283116/1‑151 (MQ=255)
                                                                 aaTCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTa                                                                             <  2:639752/149‑1 (MQ=255)
                                                                    ccaaaTCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGa                                                                         <  2:742262/147‑1 (MQ=255)
                                                                       attCTGCTCATTCAGCACACCTACATCTCGCACGGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaa                                                                      <  2:375777/148‑1 (MQ=255)
                                                                               cAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcgcg                                                             >  1:668032/1‑151 (MQ=255)
                                                                                attCAACACAACTTCAGCACGCTCTGGGTCAAAGGTAAGCAAGACTGCGGCGTGACCGCCCCAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGATGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGc                                                                 <  2:191317/144‑1 (MQ=255)
                                                                                aagcacaacaACTTCAGCGCGCACTGGGTCAAAGGGTAGAAAGGCGGGGGCGGGACCGGGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGa                                                            <  1:920972/144‑1 (MQ=255)
                                                                                aaTCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcgcg                                                             >  1:141593/1‑150 (MQ=255)
                                                                                              catcaCGCACGGGGTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtct                                                <  2:260518/149‑1 (MQ=255)
                                                                                                tcaCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTg                                            >  2:346810/1‑151 (MQ=255)
                                                                                                  ccGCACGGGTTCAAAGGGTAGAAAGCCTGCGGGGTGACCGCCCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtct                                                <  2:510990/144‑1 (MQ=255)
                                                                                                      aCTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaata                                       >  2:518075/1‑150 (MQ=255)
                                                                                                         gggTAAAAGGGTAGCAAGACGGCGGCGTGACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAAtt                                    <  1:507972/150‑1 (MQ=255)
                                                                                                          ggTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTc                                  >  2:768938/1‑151 (MQ=255)
                                                                                                            tCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCt                                 >  1:395314/1‑150 (MQ=255)
                                                                                                             cAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTc                                  >  1:717606/1‑148 (MQ=255)
                                                                                                               aaGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACAt                             >  2:533842/1‑151 (MQ=255)
                                                                                                                              gcggggTGCCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAAt               <  1:556566/150‑1 (MQ=255)
                                                                                                                              gcggcgTGACCGCCCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCaaa                <  2:875524/149‑1 (MQ=255)
                                                                                                                              gcggcgGGCCCGCTCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATg              <  1:311252/151‑1 (MQ=255)
                                                                                                                                 gcgTGCCCGCGCAAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTaa           <  2:931997/151‑1 (MQ=255)
                                                                                                                                      aCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGc      >  1:656554/1‑151 (MQ=255)
                                                                                                                                        cgcgCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGc      >  1:81578/1‑149 (MQ=255)
                                                                                                                                          cgcTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCCAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGt  >  1:607206/1‑151 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGT  >  NC_000913/3177606‑3177894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑