Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,604,344	0	C	T	46.7%	51.5 / 32.4	60	S62F (TCT→TTT)	bglJ	bgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (12/20);  new base T (28/0);  total (40/20)
Fisher's exact test for biased strand distribution p-value = 1.07e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  NC_000913/4604198‑4604492                                                                                                                                                   |                                                                                                                                                     cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttt                                                                                                                                                   <  1:976373/150‑1 (MQ=255)  aTAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTtttttcttttt                                                                                                                                                 >  2:700101/1‑151 (MQ=255)    aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcctttt                                                                                                                                                 >  2:152628/1‑149 (MQ=255)        aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGcc                                                                                                                                            >  2:371896/1‑150 (MQ=255)        aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCa                                                                                                                                           <  2:200647/151‑1 (MQ=255)        aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCa                                                                                                                                           <  2:115051/151‑1 (MQ=255)         aaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTTcctt                                                                                                                                          >  2:579075/1‑149 (MQ=255)          aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATg                                                                                                                                         <  2:453527/151‑1 (MQ=255)               tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGCCATTTTTTTTTTTTTTGCCATgag                                                                                                                                       >  1:847791/1‑148 (MQ=255)                    agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgc                                                                                                                               <  2:345818/151‑1 (MQ=255)                       agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                            <  2:630720/151‑1 (MQ=255)                           tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTATTTTTTTTTTTTTTTCCGTGAGAAGTGGgcg                                                                                                                              >  1:664667/1‑145 (MQ=255)                            cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                              >  2:65523/1‑144 (MQ=255)                                  tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTTTTTTTTTTTTTTTTGCTTGGTGAAGTGGGCGCCGGGAggg                                                                                                                     >  1:135607/1‑147 (MQ=255)                                  tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAtt                                                                                                                  <  2:657278/150‑1 (MQ=255)                                    agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                <  1:719373/150‑1 (MQ=255)                                      agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGTGAGCGCAGAGAgggggt                                                                                                                  >  2:743924/1‑143 (MQ=255)                                      agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCtt                                                                                                             >  2:175180/1‑151 (MQ=255)                                      agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt                                                                                                             <  1:272504/151‑1 (MQ=255)                                       gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTGCATGAGAAGTGAGCGGCGAGAGGGGTTAtttt                                                                                                             >  2:525152/1‑150 (MQ=255)                                               aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgactg                                                                                                    <  1:374674/151‑1 (MQ=255)                                                     tttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGGTTTCTTGCctgactgta                                                                                                  >  1:98605/1‑145 (MQ=255)                                                      ttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTgg                                                                                              <  1:135707/150‑1 (MQ=255)                                                       tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTTAGAAGAGGGCGCTGAGAGGGAATATTTTGCCTGACTGAAATGGCg                                                                                            >  2:665462/1‑151 (MQ=255)                                                              aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTCTTTTTTCTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGATTATTTTTCCTGACTGAACTTGCGCTTAAGt                                                                                     >  2:77079/1‑151 (MQ=255)                                                               aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGtt                                                                                    <  1:547663/151‑1 (MQ=255)                                                                cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCAAGAGTAGTGAACGCAGAGAGGGATTATTTTTCCTCTCCGAACAGGCGGTTAAGgtt                                                                                   >  1:862512/1‑151 (MQ=255)                                                                 ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTc                                                                                  <  2:328659/151‑1 (MQ=255)                                                                    tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTcc                                                                                 <  2:742696/149‑1 (MQ=255)                                                                      taAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGATATTTTGCCTTACTGAACGGGCGATTAAGttt                                                                                   >  2:537218/1‑145 (MQ=255)                                                                        aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGGAGTGAGCGCCGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTCcgcgga                                                                            >  2:365516/1‑148 (MQ=255)                                                                        aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAc                                                                           <  1:143336/151‑1 (MQ=255)                                                                         gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGGGGAGGGAGCGCAGAGGGGGGTTATCTTTCCTGACTGAAATGGGGGTTAAGTTTCCGCGTAcc                                                                          >  1:24354/1‑151 (MQ=255)                                                                          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGGAGGGAGGGCCAAGCGGGGGTATCTTGCCTGACTGAACTGGAGATTAAGTTTCcgcggaacc                                                                         >  1:474121/1‑146 (MQ=255)                                                                          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTGCCTGAGAAGGGGGCGCAGAGAGGGATTATCTTTCCTGACTGGACTGGGGGTTAAGGTTCCGCGTAccc                                                                         >  1:949674/1‑151 (MQ=255)                                                                           tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc                                                                         >  1:952297/1‑150 (MQ=255)                                                                           tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTTTCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc                                                                         >  2:35355/1‑150 (MQ=255)                                                                                aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCggt                                                                   >  2:208670/1‑149 (MQ=255)                                                                                 ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCCTGGGCAGGGGGGGGAGGGAGGGATTGTCTTGGCTGACTCAAACGGCGATTAAGTTTCCGGGTACCCGGCGttt                                                                  >  2:684888/1‑151 (MQ=255)                                                                                  cTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGGGAGGGATTATCTTTCCTGACTGAAATGGCGGTTAAGTTTTCGCGGACCCGGCGttt                                                                  >  1:109360/1‑150 (MQ=255)                                                                                    ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAg                                                                <  1:134482/150‑1 (MQ=255)                                                                                    ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAg                                                                <  1:152074/150‑1 (MQ=255)                                                                                      aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTGCCCCGAGAAGAGAGCGCCGGGAGGGGTTTTCTTTCCCGACTGAACTGGGGGTTAAGTTTCCGCGGACCCCGCGTTTTGtt                                                              >  2:9991/1‑150 (MQ=255)                                                                                                    aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGGAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGGTTCCGCGTACCCGGCGTTTTGTTATTTCGggtgatga                                                >  2:739548/1‑150 (MQ=255)                                                                                                    aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAATTTTTTTTTTTTTGCCCTGAGCAGGGGGCGCCGGGAGGGGCTATCTTGCCTGACTGAACTGGGGATTAAGTTTCCGCGGAACCGGGGTTTAgttgttttgggt                                                     >  2:766519/1‑139 (MQ=255)                                                                                                     tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTGAGAAGTGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGTTTTGTTATTGCGggtg                                                    >  2:642945/1‑145 (MQ=255)                                                                                                        cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGGGGGATTATCTTTCCTGACTGAACTGGGGGTTAAGTTTTCGCGGACCCGGGGGTTAGTTTTTgggggagattgtatt                                            >  1:797199/1‑139 (MQ=255)                                                                                                         aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGa                                          <  2:91643/151‑1 (MQ=255)                                                                                                                 aTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTGCCTGAGGAGGGAGCGGAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGATTTAGTTTTCGCGGAACCGGCGGTTTGGTTTTGGGGGTGATGATATTGaatccccg                                   >  1:657518/1‑144 (MQ=255)                                                                                                                 aTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCCGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGGTTTGTTATTGCGGGTGATGATATTTAAGCTCgg                                   >  2:698940/1‑150 (MQ=255)                                                                                                                  tGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCGATTAAGTTTTCGCGGACCCGGCGTTTTGTTATTGCGGGTGATGATATTGAAGCTCGGc                                  >  2:196682/1‑150 (MQ=255)                                                                                                                   gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTg                                <  1:731687/151‑1 (MQ=255)                                                                                                                     cgcgGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGATTATCTTGCCTGACTTAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTTGTTATTGCGGGTGGTGATATTGAAGCTCGGCTg                                >  2:169927/1‑149 (MQ=255)                                                                                                                      gcgGATCTCGTTTGCGGCGGTGATTTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCGGAGAGGGGGTATATTTGCTGACTGAAATTGCGATTAAGATTTTGCGTAACCGGGGTTTTGTTATTTGGGATGATGATATTTAAGATCGGGTGttt                             >  1:450493/1‑151 (MQ=255)                                                                                                                      gcgGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGGAGTGAGCGCAGAGAGGGGTTATCTTTCCCTACTGAACTGGGGATTAAGTTTTCGCGTACCCGGCGTTTTGTTTTTGCGGGTGGTGATATTTTAACTCGGCTGGtt                             >  2:471166/1‑151 (MQ=255)                                                                                                                              cGTTTGCGGCGGTCATTTTTTCTTTTTCTTCCCTGAGAAGTGAGCGCAGAGAGGGATTATCTTTCCTGACTTAACTGGCGATTAAGTTTCCGCGGACCCGGCGTTTTGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt                     >  2:252660/1‑151 (MQ=255)                                                                                                                              cGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGtt                         <  1:40304/147‑1 (MQ=255)                                                                                                                                   gcggcgGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGc                <  2:605709/151‑1 (MQ=255)                                                                                                                                         gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc          >  2:311335/1‑151 (MQ=255)                                                                                                                                         gTCATTTTTTCTTTTTCTGCCATGAGAAGAGAGCGCAGAGAGGGCTTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc          >  1:678278/1‑151 (MQ=255)                                                                                                                                          tCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc         >  2:473838/1‑151 (MQ=255)                                                                                                                                            aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGc       >  2:127608/1‑151 (MQ=255)                                                                                                                                            aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGc       >  2:510132/1‑151 (MQ=255)                                                                                                                                                  tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc  <  1:371881/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  NC_000913/4604198‑4604492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑