Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,687	C→A	V262F (GTT→TTT)	sapB ←	antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,687	0	C	A	100.0%	182.8 / NA	48	V262F (GTT→TTT)	sapB	antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/26);  total (22/26)

GCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATA  >  NC_000913/1354551‑1354831                                                                                                                                         |                                                                                                                                                 gCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGatc                                                                                                                                     >  2:765157/1‑150 (MQ=255)  cACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGACATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGatca                                                                                                                                    >  2:958858/1‑150 (MQ=255)     ccAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGc                                                                                                                                <  2:538701/151‑1 (MQ=255)       aaaaTATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGcc                                                                                                                               >  2:279659/1‑150 (MQ=255)       aaaaTATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCa                                                                                                                              <  1:135391/151‑1 (MQ=255)       aaaaTATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCa                                                                                                                              <  2:323242/151‑1 (MQ=255)              cAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGt                                                                                                                          >  2:389946/1‑148 (MQ=255)              cAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTc                                                                                                                         >  2:441276/1‑149 (MQ=255)                gAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGc                                                                                                                      >  2:714166/1‑150 (MQ=255)                gAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCa                                                                                                                     <  2:764984/151‑1 (MQ=255)                   aTCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCAtgg                                                                                                                  <  1:793715/151‑1 (MQ=255)                   aTCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCAtgg                                                                                                                  >  2:495314/1‑151 (MQ=255)                        gTTAACAATAATGACCAGTGAGCCACCCACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGCTCATCGCCAGCGTCAGCATGGTGGaa                                                                                                             <  2:360279/151‑1 (MQ=255)                           aaCAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAAc                                                                                                          >  1:418169/1‑151 (MQ=255)                               ataatGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCCGCGTCAGCATGGTGGAAAACTGt                                                                                                       >  1:192413/1‑150 (MQ=255)                               ataatGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTa                                                                                                      <  1:839811/151‑1 (MQ=255)                                 aatGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAg                                                                                                    <  1:800343/151‑1 (MQ=255)                                       cAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAgg                                                                                              >  1:20651/1‑151 (MQ=255)                                        aGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGc                                                                                             >  2:465012/1‑151 (MQ=255)                                          tGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCg                                                                                            >  2:20046/1‑150 (MQ=255)                                           gAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCg                                                                                            >  1:20343/1‑149 (MQ=255)                                                acacacCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAAt                                                                                      >  2:330358/1‑150 (MQ=255)                                                acacacCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATa                                                                                     <  2:588072/151‑1 (MQ=255)                                                 cacacCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATa                                                                                     <  1:721840/150‑1 (MQ=255)                                                   cacCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAAcc                                                                                  <  1:133778/151‑1 (MQ=255)                                                        tCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGGGGAAAACTGTAAGCCCAGGCGAGGAATAACcggcgg                                                                             >  2:943481/1‑151 (MQ=255)                                                          aCTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGt                                                                            >  2:754144/1‑150 (MQ=255)                                                           cTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTa                                                                           <  1:464998/150‑1 (MQ=255)                                                              cggcggAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAAcgc                                                                       <  2:126578/151‑1 (MQ=255)                                                                 cggAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGACAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGtt                                                                    <  2:512939/151‑1 (MQ=255)                                                                        ggCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGc                                                                >  2:275856/1‑148 (MQ=255)                                                                             cGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTg                                                        >  1:315803/1‑151 (MQ=255)                                                                             cGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTg                                                        <  2:435780/151‑1 (MQ=255)                                                                                 aTCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGa                                                    <  1:862148/151‑1 (MQ=255)                                                                                                       attaaCCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACa                              <  1:704142/151‑1 (MQ=255)                                                                                                        ttaaCCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACa                              >  1:761802/1‑150 (MQ=255)                                                                                                         taaCCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACaa                             <  1:20407/150‑1 (MQ=255)                                                                                                          aaCCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACaaa                            <  2:334685/150‑1 (MQ=255)                                                                                                           aCCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAAcc                          <  1:979074/151‑1 (MQ=255)                                                                                                           aCCAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAAc                           <  2:767805/150‑1 (MQ=255)                                                                                                            ccAGCGCCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACcg                         <  1:763704/151‑1 (MQ=255)                                                                                                               gcgcCCTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCgg                      <  1:697991/151‑1 (MQ=255)                                                                                                                    cTAAACCCGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCACAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTcgc                 >  1:822789/1‑151 (MQ=255)                                                                                                                      aaaCCCTGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTcgcca               <  1:559087/151‑2 (MQ=255)                                                                                                                         cccGGCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTcgccgccg            <  1:765133/151‑1 (MQ=255)                                                                                                                             gCCAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCttt        <  1:142281/151‑1 (MQ=255)                                                                                                                               cAGCTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCtttt       >  1:943512/1‑150 (MQ=255)                                                                                                                                  cTAAAAAACATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACa    >  1:910634/1‑150 (MQ=255)                                                                                                                                    aaaaaaCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACAt   >  2:432859/1‑149 (MQ=255)                                                                                                                                    aaaaaaCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATa  <  1:943452/150‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                 GCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATA  >  NC_000913/1354551‑1354831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑