| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,400,673 | A→C | F139V (TTC→GTC) | mreB ← | cell wall structural complex MreBCD, actin‑like component MreB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,400,673 | 0 | A | C | 98.3% | 221.9 / ‑3.4 | 58 | F139V (TTC→GTC) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (25/32); total (25/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-01 | |||||||||||
CACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGA > NC_000913/3400527‑3400816 | cacaGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACt > 1:680491/1‑151 (MQ=255) agaagaGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCa < 1:718620/151‑1 (MQ=255) aggagTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGc < 2:835187/151‑1 (MQ=255) aggagTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGc < 2:592690/151‑1 (MQ=255) gagTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCAc > 1:17918/1‑151 (MQ=255) agTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCAcc > 1:594154/1‑151 (MQ=255) gTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCa < 1:958323/151‑1 (MQ=255) aaCCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAgcg > 2:605435/1‑151 (MQ=255) acCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTgc < 1:226619/151‑1 (MQ=255) ccGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTgcg < 1:397009/151‑1 (MQ=255) tCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCTAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCgg > 1:435923/1‑150 (MQ=255) cAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGAt > 2:291943/1‑151 (MQ=255) cAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGAt < 1:883974/151‑1 (MQ=255) ggAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCa < 1:493886/151‑1 (MQ=255) aaCAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAAt < 1:844567/150‑1 (MQ=255) aaCAGCAACTTCAGAGGTACCACCACCGATATCAACCACCATACAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGGTTCACGAAt > 1:590457/1‑150 (MQ=255) cAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgc > 1:316261/1‑151 (MQ=255) aGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACCTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgc > 1:380267/1‑150 (MQ=255) cAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGcgcg < 2:387469/151‑1 (MQ=255) aaCTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGAACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCgcgg > 1:560324/1‑151 (MQ=255) tCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCATCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGt > 2:922632/1‑150 (MQ=255) tCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCgcggcg < 2:546227/149‑1 (MQ=255) aGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCa < 2:323862/151‑1 (MQ=255) accaccaccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTgg > 1:611367/1‑151 (MQ=255) ccaccaccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTggg > 2:553630/1‑151 (MQ=255) ccaccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTcg > 1:231481/1‑151 (MQ=255) ccaccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTc < 1:650382/150‑1 (MQ=255) accGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTcgcg > 1:531677/1‑151 (MQ=255) accGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTcgcg > 2:555383/1‑151 (MQ=255) gATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCa < 1:819782/151‑1 (MQ=255) tatCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAAc > 2:955766/1‑151 (MQ=255) cAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAAcca < 1:788534/150‑2 (MQ=255) accaccATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGca < 2:326965/151‑1 (MQ=255) accaccATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGca < 1:329205/151‑1 (MQ=255) ccaccaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGcac > 2:828019/1‑151 (MQ=255) aGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAAcc > 2:763181/1‑151 (MQ=255) aGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAAcc < 2:110895/151‑1 (MQ=255) aaCCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAg < 1:685209/151‑1 (MQ=255) aaCCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAg < 2:414284/151‑1 (MQ=255) cGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcgcgc < 1:957668/151‑1 (MQ=255) cGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcgcg < 1:621845/150‑1 (MQ=255) aGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCtt < 1:3965/151‑1 (MQ=255) aaaCCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTgg > 1:336307/1‑151 (MQ=255) aCCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGAc < 2:17917/151‑1 (MQ=255) aCCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGAc < 1:584184/151‑1 (MQ=255) cAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATa < 1:123614/151‑1 (MQ=255) gCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATaaa < 1:31419/150‑1 (MQ=255) cACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGtt < 1:776140/151‑1 (MQ=255) aCCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTg > 1:369163/1‑151 (MQ=255) aTTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCtgt < 2:680513/151‑1 (MQ=255) tGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGc > 1:50645/1‑151 (MQ=255) tGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGc > 1:647818/1‑151 (MQ=255) gCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGc > 1:734615/1‑150 (MQ=255) gCCATCGGTTCTTCAACCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGt < 1:651763/150‑1 (MQ=255) cATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtg < 1:605412/151‑1 (MQ=255) tcAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTg < 1:807536/149‑1 (MQ=255) aTCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGa < 1:264995/151‑1 (MQ=255) tCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTgg > 1:828827/1‑149 (MQ=255) | CACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGA > NC_000913/3400527‑3400816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |