Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,440,923	C→T	G36D (GGC→GAC)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,440,923	0	C	T	100.0%	326.1 / NA	84	G36D (GGC→GAC)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/41);  total (43/41)

CAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAG  >  NC_000913/3440775‑3441069                                                                                                                                                     |                                                                                                                                                   cAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCa                                                                                                                                                  <  2:482172/151‑1 (MQ=255)  aGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCaa                                                                                                                                                 <  1:253736/151‑1 (MQ=255)  aGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCa                                                                                                                                                  <  1:563273/150‑1 (MQ=255)   ggTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCaaa                                                                                                                                                <  2:400087/151‑1 (MQ=255)        aGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCAc                                                                                                                                           <  2:965402/151‑1 (MQ=255)          cAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGc                                                                                                                                         >  2:311746/1‑151 (MQ=255)            ggATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGctc                                                                                                                                       >  1:478519/1‑151 (MQ=255)               tttCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTa                                                                                                                                     >  2:925434/1‑150 (MQ=255)                    aGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGc                                                                                                                                >  1:167971/1‑150 (MQ=255)                    aGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCt                                                                                                                               <  2:958553/151‑1 (MQ=255)                            ttCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                                       >  2:834721/1‑151 (MQ=255)                             tCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAggg                                                                                                                         <  2:820388/148‑1 (MQ=255)                             tCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                                       >  2:810473/1‑150 (MQ=255)                              ccTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCAc                                                                                                                     <  2:96713/151‑1 (MQ=255)                               cTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCAcc                                                                                                                    >  2:179210/1‑151 (MQ=255)                                 gAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCtt                                                                                                                  >  1:887231/1‑151 (MQ=255)                                 gAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCtt                                                                                                                  >  1:94100/1‑151 (MQ=255)                                 gAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCtt                                                                                                                  <  1:735526/151‑1 (MQ=255)                                 gAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCt                                                                                                                   <  1:546928/150‑1 (MQ=255)                                 gAACGCCTTCTTTGGTGCTGTACTCATGTAGCACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCtt                                                                                                                  >  1:683103/1‑151 (MQ=255)                                  aaCGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTg                                                                                                                 >  1:901196/1‑151 (MQ=255)                                     gCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcg                                                                                                              >  1:627987/1‑151 (MQ=255)                                       cttcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtg                                                                                                            >  2:264200/1‑151 (MQ=255)                                         tcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtgcg                                                                                                          >  2:545102/1‑151 (MQ=255)                                            ttGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTc                                                                                                        <  1:192919/150‑1 (MQ=255)                                            ttGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCg                                                                                                       >  1:868761/1‑151 (MQ=255)                                               gTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGaa                                                                                                     <  2:803440/150‑1 (MQ=255)                                               gTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAAc                                                                                                    >  1:260278/1‑151 (MQ=255)                                                 gCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACt                                                                                                   <  1:216012/150‑1 (MQ=255)                                                  cTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTc                                                                                                  >  1:922053/1‑150 (MQ=255)                                                   tGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCAc                                                                                                >  2:831712/1‑151 (MQ=255)                                                    gTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCa                                                                                                 <  2:612132/149‑1 (MQ=255)                                                      aCTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACtt                                                                                              >  1:126102/1‑150 (MQ=255)                                                      aCTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACtt                                                                                              <  1:693150/150‑1 (MQ=255)                                                         cATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGc                                                                                            <  1:75488/149‑1 (MQ=255)                                                            gtagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGat                                                                                       >  2:670843/1‑151 (MQ=255)                                                               gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATc                                                                                    >  2:722802/1‑151 (MQ=255)                                                                  cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAc                                                                                 >  1:770200/1‑151 (MQ=255)                                                                  cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAc                                                                                 <  2:170540/151‑1 (MQ=255)                                                                      aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAgg                                                                             >  2:613754/1‑151 (MQ=255)                                                                         aaTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcgc                                                                          <  2:573553/151‑1 (MQ=255)                                                                         aaTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcgc                                                                          <  2:515528/151‑1 (MQ=255)                                                                          aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGcgcg                                                                         >  2:805063/1‑151 (MQ=255)                                                                              cAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGttt                                                                     <  1:755038/151‑1 (MQ=255)                                                                              cAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGtt                                                                      <  1:826384/150‑1 (MQ=255)                                                                                 ccTCGGTCACCGCGCATCCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTa                                                                   <  1:195292/150‑1 (MQ=255)                                                                                 ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGtttt                                                                    >  2:879915/1‑149 (MQ=255)                                                                                    cGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGaa                                                                <  2:73415/150‑1 (MQ=255)                                                                                     ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAAc                                                              >  2:964459/1‑151 (MQ=255)                                                                                           cgcgCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTc                                                        <  2:543588/151‑1 (MQ=255)                                                                                            gcgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTca                                                       <  2:803158/151‑1 (MQ=255)                                                                                            gcgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTc                                                        >  2:843702/1‑150 (MQ=255)                                                                                            gcgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTc                                                        >  1:268504/1‑150 (MQ=255)                                                                                             cgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcac                                                      <  1:544586/151‑1 (MQ=255)                                                                                                     ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCt                                              <  1:938636/151‑1 (MQ=255)                                                                                                       cATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGc                                            <  2:294159/151‑1 (MQ=255)                                                                                                        aTCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCa                                           <  1:670820/151‑1 (MQ=255)                                                                                                          cGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAtt                                         <  2:587553/151‑1 (MQ=255)                                                                                                          cGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAtt                                         <  1:852326/151‑1 (MQ=255)                                                                                                             tGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtgtg                                      >  1:202856/1‑151 (MQ=255)                                                                                                                 agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                  >  2:823220/1‑151 (MQ=255)                                                                                                                 agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                  >  2:449916/1‑151 (MQ=255)                                                                                                                  gCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctc                                 <  2:546128/151‑1 (MQ=255)                                                                                                                   cAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctc                                 <  1:688700/150‑1 (MQ=255)                                                                                                                    aGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctc                               >  1:687601/1‑151 (MQ=255)                                                                                                                      aaTACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                             <  2:683125/151‑1 (MQ=255)                                                                                                                      aaTACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                             <  1:781562/151‑1 (MQ=255)                                                                                                                       aTACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt                            >  2:262319/1‑151 (MQ=255)                                                                                                                        tACTGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTg                           >  2:405671/1‑151 (MQ=255)                                                                                                                         aCGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTg                           <  1:217624/150‑1 (MQ=255)                                                                                                                          cGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACTAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGt                         <  1:810441/151‑1 (MQ=255)                                                                                                                                aGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTa                     >  2:61697/1‑149 (MQ=255)                                                                                                                                  tGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCt                 >  2:732305/1‑151 (MQ=255)                                                                                                                                   gCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCtt                >  1:216340/1‑151 (MQ=255)                                                                                                                                   gCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCtt                <  1:311732/151‑1 (MQ=255)                                                                                                                                   gCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCtt                <  2:167973/151‑1 (MQ=255)                                                                                                                                    cGTTACCCAGAGTATGGTAAAAGCCAAGCTCTAAAGGCTCAAGGAACACCTTGGCGTGCGTCGATCTCGCTTGCACGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAATCGTGCATTGTGTCGTCTCTTTGGTACTAAGc                  <  1:688969/148‑1 (MQ=255)                                                                                                                                        aCCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt           >  1:654187/1‑151 (MQ=255)                                                                                                                                         cccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTg          >  2:728868/1‑151 (MQ=255)                                                                                                                                         cccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTg          >  2:682864/1‑151 (MQ=255)                                                                                                                                             gagTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGt      >  2:709101/1‑151 (MQ=255)                                                                                                                                              agTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGt      >  2:568417/1‑150 (MQ=255)                                                                                                                                              agTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGt      >  1:109843/1‑150 (MQ=255)                                                                                                                                                 aTGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAg  <  2:895750/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAG  >  NC_000913/3440775‑3441069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑