Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,921,173	0	T	C	45.0%	49.8 / 10.3	40	I310V (ATT→GTT)	gudP	putative D‑glucarate transporter
Reads supporting (aligned to +/- strand):  ref base T (19/3);  new base C (0/18);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.19e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGAC  >  NC_000913/2921032‑2921322                                                                                                                                              |                                                                                                                                                      ccATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATcccaccc                                                                                                                                                >  1:567838/1‑149 (MQ=255)   aTAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGca                                                                                                                                            <  1:736220/151‑1 (MQ=255)   aTAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGc                                                                                                                                             >  2:560044/1‑150 (MQ=255)         ccGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACAccgc                                                                                                                                      <  1:37979/151‑1 (MQ=255)             tgatCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGAt                                                                                                                                  >  2:84127/1‑151 (MQ=255)                            cGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAaaaccgcaaaccgc                                                                                                                   >  2:331153/1‑151 (MQ=255)                               tGACGAAGTTGCAGAACCCCACCCCCATCGACAGAAACATCCCCATTCCGACCGTTTTTTCCCGCCCAATGTCCGGCGACCCCGGGCGGCGAATCAGCCAGCCGGAAATAATCCCCCCCATCACACCGCCGATAAAACCGCAAAccgcc                                                                                                                  <  1:63332/149‑1 (MQ=255)                                 aCGTAGTTGCAAAACCCCACCACCTCCGACAGCACCATGCCCATTACGACCGGTGTTTTACGCGAAATGTCCAGCGACCCCGGGCGGCGCAACAGCCAGTCGGAAATAACCCCACCCAGAACACCGCCGATAAAACCGCAAACCGCcggaa                                                                                                              <  2:636586/151‑1 (MQ=255)                                  cGTAGTGGCAAACCCCCACCCCCACCGACAGCAACTTCCCCTTTACAACCGGTTTTTTCCGCGCAATGTTCAGCGACCCCGTGCGGCGCATCAGCCAGTCGGAAATAACCCCCCCCAGCACCCCGCCGATAAAACCGCAAACCGCcgga                                                                                                               <  2:256725/149‑1 (MQ=255)                                     aGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacgg                                                                                                           >  1:333924/1‑150 (MQ=255)                                        tccaaaaCCCCACCCCCATCGACAGCAACATCCCCATTACAACCGGGTTTTTCCGCCCATTGTTCAGCGACCCCGTGCGGCGCACCACCCGGCGGGAAAAAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAg                                                                                                        <  2:703656/145‑1 (MQ=255)                                         gCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGcc                                                                                                      >  1:538695/1‑151 (MQ=255)                                          cAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGAGGCGCATCAGTCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGAAAACCGCCGGAACGGAAGcc                                                                                                      >  1:571563/1‑150 (MQ=255)                                             aaCACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCcaca                                                                                                   >  1:516931/1‑150 (MQ=255)                                              acacCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACaa                                                                                                  >  1:104697/1‑150 (MQ=255)                                                  catcaccaccGACAGCAACATGCCCATTCCGGTGGGTGTTTTCCGCGCAATGTTCAGCGACCCGGTGCGGCGCATCAGCCAGTCGGAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCGCAAAGccc                                                                                             <  2:492050/151‑1 (MQ=255)                                                        caccGACAGAAAAATGCCCATTACGAACGGTTTTTTCCGCGAATTGTCCAGCGCCCCGGTGCGGCGCACCGGCCAGTGGAAAAAAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTc                                                                                       <  2:758139/148‑1 (MQ=255)                                                              cAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATc                                                                                 >  1:231784/1‑151 (MQ=255)                                                               aGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCg                                                                                >  1:381104/1‑151 (MQ=255)                                                                  aaCATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacg                                                                                                            >  1:519019/1‑120 (MQ=255)                                                                       ccccatacctaccGGTTTTTTACGCGAAAGGTACACCGCTCCCGCGGGGCCCACCACCCGGCCGGAAAAACCCCCCCCCAGCACCCCGCCGATAAAACCGCAACCCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcg                                                                        <  1:669124/139‑1 (MQ=255)                                                                        cccATTACGATCGGTTTTTTACGCGAAATGTTCAGCGCCCCCGTGCGGCGCATCAGCCAGTCGAAAATAACCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATccc                                                                         <  2:122463/149‑1 (MQ=255)                                                                        ccaaTTACGATCGTTGTTTTCCGCGAATTGTTCAGCGTCCCGGTGCGGCCCATCACCCGTTCGAAAATATCCCCCCCCACCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATccc                                                                         <  2:567799/146‑1 (MQ=255)                                                                                        ttttCCGCGCATTGTTCACCACCCCCGTGCGGCGCACCAGCCATTCGAAAATAACCCCACCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCa                                                              <  2:153442/144‑1 (MQ=255)                                                                                           tACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATaaa                                                     >  1:65166/1‑150 (MQ=255)                                                                                             cgcgCAATGCTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGACCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCg                                                  >  1:486376/1‑151 (MQ=255)                                                                                                acattGTCCAGCGCCCCCGTGCGGCCCACCAGCCAGCGGAAAATACCCCCCCCCACCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGa                                               <  1:94183/147‑1 (MQ=255)                                                                                                 cAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGa                                               >  1:788458/1‑150 (MQ=255)                                                                                                  aaTGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAAc                                             >  1:694397/1‑151 (MQ=255)                                                                                                     gTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAAcc                                            >  1:3667/1‑149 (MQ=255)                                                                                                        cAGCGACCCCGTGCGGCACACCACCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAg                                          <  2:132048/148‑1 (MQ=255)                                                                                                               cccGGGCGGCGAACCAGCCAGTCGGAAATAACCCCCCCCAGCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAaag                                 <  2:220325/150‑1 (MQ=255)                                                                                                                ccGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAaaga                                >  2:167696/1‑150 (MQ=255)                                                                                                                ccGTGCGGCGCATCAGCCAGCCGAAAATAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAaagaa                               <  2:455482/151‑1 (MQ=255)                                                                                                                    gcggccCACCACCCAGTCGAAAAAAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagtaa                           <  1:778784/151‑1 (MQ=255)                                                                                                                            ccacccAGTGGAAAAAAACCCCCCCCACCCCCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAggg                    <  2:281647/146‑1 (MQ=255)                                                                                                                             cAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCg                  >  2:766909/1‑151 (MQ=255)                                                                                                                                caagccGGAAAAAACCCCCCCCAGCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCg                  <  1:513484/143‑1 (MQ=255)                                                                                                                                    tCGAAAAAAACCCCCCCCAGCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGt                 <  2:244812/145‑1 (MQ=255)                                                                                                                                       aaaaaaaCCCCCCCCGCCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGt                 <  1:617268/141‑1 (MQ=255)                                                                                                                                          ataatCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACt     >  1:232713/1‑151 (MQ=255)                                                                                                                                            aatCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTg    >  1:109395/1‑150 (MQ=255)                                                                                                                                             atCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGAc  >  1:663827/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                      CCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGAC  >  NC_000913/2921032‑2921322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑