Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,864,0990CA42.1% 56.8 / 13.3 39R67R (CGC→CGA) yidPUTRA domain‑containing GntR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (16/6);  new base A (0/16);  total (16/23)
Fisher's exact test for biased strand distribution p-value = 4.62e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

GATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATCAACG  >  NC_000913/3863950‑3864247
                                                                                                                                                     |                                                                                                                                                    
gATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTgcgc                                                                                                                                                      >  2:205507/1‑150 (MQ=255)
        gCTAAACAGCCTTCTTCCCGGTGAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGt                                                                                                                                              >  2:669850/1‑150 (MQ=255)
           aaaCAGCCTTCTTCCCGGTGAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTg                                                                                                                                          <  1:489431/151‑1 (MQ=255)
            aaCAGCCTTCTTCCCGGTGAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGc                                                                                                                                         <  1:745461/151‑1 (MQ=255)
                     cttcCCGGTGAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCg                                                                                                                                >  2:749355/1‑151 (MQ=255)
                        cccGGTGAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGcc                                                                                                                              >  1:144758/1‑150 (MQ=255)
                               aaaaaaaGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGAc                                                                                                                      >  1:254459/1‑151 (MQ=255)
                               aaaaaaaGCGGGCGGAAGATTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTGGCGGGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGAc                                                                                                                      <  2:221663/151‑1 (MQ=255)
                                       cTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCtgg                                                                                                              >  1:652702/1‑151 (MQ=255)
                                                   tgtGCGTTATGGGGAATGACCACCCGAAAGGCCATTGCCCGGCTGGTGGGGGGGGGGTGGGTGGCCCGCCGCCCCGGGGGTGGCACTTCCCGGGGGCGAAAAGATGTACTGCATCAACCCGCCAGTCTGACCGGGCTGGTGGAGgtg                                                                                                      <  1:453104/145‑1 (MQ=255)
                                                          tcccgggggagACCACCCGAAAAGCCATTTCCCGGCTGGGTGGGTGGGGGCTGGTGCTCCGCCGCCCCGGTGGGGGCACTTCCCTGGTGCGAAAATATGTTCGGCATAAAACCGCCAGTCTGACCGGGCTGGTGGAg                                                                                                         <  1:228436/127‑1 (MQ=255)
                                                          tATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACgg                                                                                             >  1:54998/1‑149 (MQ=255)
                                                           aTCGGGGATGACCATCCGAAAAGCCATTGCCCGGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCCGGGTAGGGGCACTTACCTGGTGCGAAAAGATGTACTGCATAAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCa                                                                                           <  2:772819/150‑1 (MQ=255)
                                                                ggATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGaa                                                                                      >  2:786432/1‑150 (MQ=255)
                                                                      ccagcggaaaaGCCATTGCCCGGCTGGTAGCGGGGGGGCGGGTGGCCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGAAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACg                                                                                 <  1:602764/141‑1 (MQ=255)
                                                                          ccGAAAAGCCATTGCCCGGCTGGTGGCGGGGGGGCTGGTGGCCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGAAAAGATGTACGGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCa                                                                             <  2:47523/149‑1 (MQ=255)
                                                                                   cATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAgg                                                                    >  1:589087/1‑149 (MQ=255)
                                                                                       gACCTGCTGGTGGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTCCCTGGTGCGAAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGa                                                              <  2:535420/151‑1 (MQ=255)
                                                                                        cccTGCTGGGAGCGGGGGGGGTGGTGGCCCGCCGCCACGGTGGTGGCACTTCCCTGGTGCGAAAAGATGTCCTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGAt                                                             <  2:752278/150‑1 (MQ=255)
                                                                                        aCCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGAt                                                             >  1:652829/1‑151 (MQ=255)
                                                                                         ccggttgtgggggTGGGGGGTGGGGTGCCGCCCCCCCGTTGTTGGCTCTTCCCGGGTGCGAAAAGATGTACGGCACAAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCt                                                                <  1:688313/135‑1 (MQ=255)
                                                                                               ggTAGGGTGGGGGGGGGGGGTCCGCCCCCACGGTGGTGGCACTTACCTGGGGCGAAAAGATGTACTGAACCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGAttttt                                                         <  1:300999/148‑1 (MQ=255)
                                                                                               ggTAGCGGGGGGGCGGGTGGTCCCCCGCCACGGTGGGGGCATTTACCTGGTGCGAAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGAttttt                                                         <  1:683029/148‑1 (MQ=255)
                                                                                                 tAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAAt                                                    >  2:73172/1‑151 (MQ=255)
                                                                                                   gggggggggCTGGTGGCCCGCCGCCAGGGTAGGGGCACTTACCTGGTGTGAAAAGATGTAATGAAACAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGaaa                                                     <  1:161938/144‑1 (MQ=255)
                                                                                                      tGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGc                                               >  1:663922/1‑151 (MQ=255)
                                                                                                         gggggggtggcCCGCCGCCACGGTGGTGGCATTTCCCTGGTGCGAAAAGATGTACTCCATAAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTg                                            <  1:499077/146‑1 (MQ=255)
                                                                                                              ggtgtcccgccgCCACGGTGGTGGCACTTGCGGGGTGCGAAAAGATGTCCTGCATAAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGG‑AAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCt                                       <  1:244000/144‑1 (MQ=255)
                                                                                                                   tCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGc                                  >  2:73072/1‑151 (MQ=255)
                                                                                                                    cccccgccAGGGTATGGGCACTTCCCTGGTGCGCAACGATGTACGCCATCAACCCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGcc                                 <  1:799469/148‑1 (MQ=255)
                                                                                                                    cccccgccACGGTAGTGCCCCTTCCCTGGGGGGCAAAGTTGTACTGAATAAAGCCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTcc                                     <  2:110240/144‑1 (MQ=255)
                                                                                                                    ccccccccACGGTAGTGGCACTTACCTGGTGCGAAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGcc                                 <  2:429683/145‑1 (MQ=255)
                                                                                                                        cgccACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTg                             >  1:539270/1‑151 (MQ=255)
                                                                                                                        cgccACGGGGGTGGCAGTTACGGTGGGCGTAATAATATGCTGAATAAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCATGCGCCTCCg                                    <  1:506129/144‑1 (MQ=255)
                                                                                                                          ccACGGTAGGGGCACTTCCCTGGTGCGAAAAGATGTACTGCATAAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTgc                            <  2:233219/150‑1 (MQ=255)
                                                                                                                           cACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGGTTTTTGAAATCATGCCTGCGCCTCCGGCCATTgcc                           >  1:130968/1‑150 (MQ=255)
                                                                                                                           cACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTgcc                           >  1:502634/1‑150 (MQ=255)
                                                                                                                                  gTGGCCCTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGtt                   <  2:652024/151‑1 (MQ=255)
                                                                                                                                      cACTTCCCTGGTGCGAAAAGATGTACTGCATAAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACg                <  2:798063/150‑1 (MQ=255)
                                                                                                                                              tGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAAt       >  2:404784/1‑151 (MQ=255)
                                                                                                                                                  gcgaAAAGATGTACTGCATAAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATCaa    <  2:521348/150‑1 (MQ=255)
                                                                                                                                                    gcAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATCAACg  >  2:480433/1‑150 (MQ=255)
                                                                                                                                                     cAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATCAACg  >  1:174193/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GATTTCACGCTAAACAGCCTTCTTCCCGGTGAAAAAAAGCTGGCGGAAGAGTTTGCGGTATCGCGGATGACCATCCGTAAAGCCATTGACCTGCTGGTAGCGTGGGGGCTGGTGGTCCGCCGCCACGGTAGTGGCACTTACCTGGTGCGCAAAGATGTACTGCATCAAACCGCCAGTCTGACCGGGCTGGTGGAGGTGTTAAAACGGCAGGGAAAAACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATCAACG  >  NC_000913/3863950‑3864247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑