Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,222,596	0	G	A	45.7%	26.2 / 22.4	35	F13F (TTC→TTT)	ymgD	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (18/1);  new base A (0/16);  total (18/17)
Fisher's exact test for biased strand distribution p-value = 4.19e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGAC  >  NC_000913/1222481‑1222739                                                                                                                     |                                                                                                                                                 ctccagccTGTGCGCTGCCTTTTTTTGCCTTTTCTTTGGTTTCTGCAAAATTAATATAGTTTTCTGACGGCAACCCTTTTTT‑AATTTTGTGTTCCTGCGCCAAGTTCATTGGGGAAAAAAAAAACAGG‑CCCGCCAGTAAAGCAAAttt                                                                                                                 <  1:173833/142‑1 (MQ=255)   gCACCCTGTGCGCTGTCTTTTTTTGCCTTTTCGTTGGTTTCTGCGCAAGAAATATGGGTTTCTGACGGAAACCCTTTTTT‑AATATTTTAGTCCTGCCCCCAGGTCTTTGGTGCAAAAACAAACGGG‑CCCGCCAGTAAAGCAAATTTTTtc                                                                                                             <  2:738795/150‑1 (MQ=255)     acccttggcgcTTTCATTTTTGGCTTTTTTTTTGGTTTCTGCGCAAGTAATTTAGTTTTCTGACGCCAACCTTTTTTT‑AATATTGTATTCCGCCGCCCAGGCCTTGGGTGCAAAAAAAAACGGG‑CCCGCCAGTAAAGCAAATTTTTtc                                                                                                             <  2:431280/141‑1 (MQ=255)     acccTGGGCGCTGTCATTTTTTCCCTTTTCGTTGGTTTCTGCGAAAAAAATATAGGTTTCTGAGGGCAACCCTTTTTT‑AATTTTGTAGCCCTGCCCCCAGGTCATGGGTGAAAAAAAAAACAGG‑CCCGCCAGTAAAGCAAATTTTTtcatc                                                                                                          <  2:271181/149‑1 (MQ=255)          gtgCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTcatcatcat                                                                                                     >  2:722076/1‑151 (MQ=255)            gcgcTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTc                                                                                                   >  1:529115/1‑151 (MQ=255)                     ttttttCCCATTTGGTTGGTTTCTGCGCAATTAATATGGTTTTCGGCCGGCAAACGTTTTTT‑AATTTTGTGGTCCTGCCCCCAGTTAATTGGTGAAAAAAAAAACAGC‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCtt                                                                                                <  2:469578/144‑1 (MQ=255)                      tttttGCTTTTTCTTTGGTTTCTGCACAAATAATATAGTTTTCTGACGGCAAACCGTTTTT‑AATATTGTATCCCTGCCCCCAGGTCATTGGTGCAAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCtt                                                                                                <  2:433218/144‑1 (MQ=255)                       ttttGCCTTTTCGTTGGTTTCTGCGAAAGTAATATAGGTTTCTGACGGCAAACCTTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCAAAAACAAACGGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAt                                                                                         <  2:410021/150‑1 (MQ=255)                       ttttCCTATTTCGTTGTTTTGTGCGCAAGTAATATATTTTTCTGACGACAACCCTTTTTT‑AATATGATACGCTAGCGCCAAGTTTTTGGGTGCAAAAAAAAATTGC‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTc                                                                                           <  1:139513/148‑1 (MQ=255)                        tttGCCTTTTCGTTGGCTTCTGCGAAAGTAATATAGTTTTCTGACGACAACCCTTTTTT‑ATTATTGTAGTCCGGCCCCCGGGTCATGGGTGCAAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAtt                                                                                        <  2:558369/150‑1 (MQ=255)                             cATCTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCACAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTATTTCATCATTATTCCTTATTTCATTTTAcc                                                                                   >  1:29768/1‑150 (MQ=255)                               tttCGTTGTTTTCTGCGCAATAAATATAGTTTTCTGCGGGCAACCCTTTTTA‑AATTTTGTAGTCCTGCCCCCAGTTCTTTGGTGCAAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCa                                                                                 <  1:397633/150‑1 (MQ=255)                                   gTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAAACGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAtt                                                                            >  1:700559/1‑151 (MQ=255)                                    ttGGTTTCTGCGCAAGTAATATAGTTTTCTGACGGAAACCCTTTTTT‑AATTTTGTACTCCTGCGCCCAGGTCATTGGTGCAAAAAAAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGaa                                                                              <  2:185302/148‑1 (MQ=255)                                      ggCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGc                                                                          >  2:296031/1‑150 (MQ=255)                                          tCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcaccac                                                                     >  1:282648/1‑151 (MQ=255)                                                 aaGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTAcc                                                              >  2:547651/1‑151 (MQ=255)                                                    tAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGcc                                                           >  2:294355/1‑151 (MQ=255)                                                     aaTATGGTTTTCTGCCGG‑AAACCTTTTTTAAATATTGTTGTCCGGCCCCTAGTTCTTTGGTG‑GAAAAAAAACCGGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCa                                                          <  1:696674/151‑1 (MQ=255)                                                        ataGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATc                                                       >  1:431361/1‑151 (MQ=255)                                                           ggTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACg                                                    >  2:644797/1‑151 (MQ=255)                                                              ttCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGc                                                  >  1:754719/1‑150 (MQ=255)                                                                        aaccttttttt‑AATTTTGTACCCCTGCCCCCAGTTCATTGGTGCAAAAAAAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATc                                        <  2:361529/144‑1 (MQ=255)                                                                            cGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGcccc                                   >  2:458180/1‑151 (MQ=255)                                                                               tttt‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTc                                >  2:591041/1‑151 (MQ=255)                                                                                    aaTATTGTAGTCCTGCCCCCAGGTCTTTGGTGCAAAAAAAAACAGC‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCg                               <  2:482468/148‑1 (MQ=255)                                                                                       atTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGcc                          >  1:711411/1‑150 (MQ=255)                                                                                                 tGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCATCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGt                 >  1:739102/1‑149 (MQ=255)                                                                                                   cccccAGGTCATGGGTGAAAAAAAAAACGGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGa             <  2:711349/149‑1 (MQ=255)                                                                                                         gttctttGGGGCAAAAAAAAACCGC‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAAc           <  1:127415/142‑1 (MQ=255)                                                                                                           tCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGt     >  2:494356/1‑151 (MQ=255)                                                                                                             aTTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTg    >  2:365795/1‑150 (MQ=255)                                                                                                             aTTGGTCCAAAAAAAAACAGC‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGt     <  2:132844/149‑1 (MQ=255)                                                                                                                ggTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGAc  >  1:325588/1‑149 (MQ=255)                                                                                                                     |                                                                                                                                                 CTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGAC  >  NC_000913/1222481‑1222739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑