Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,356,297 A→T W272R (TGG→AGG)  sapA ← antimicrobial peptide transport ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,356,2970AT97.3% 126.2 / ‑5.2 38W272R (TGG→AGG) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (20/16);  minor base C (1/0);  total (21/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGC  >  NC_000913/1356151‑1356440
                                                                                                                                                  |                                                                                                                                               
cAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGGTAAATGCCAGATAGGCGACGTTCATCCCCGGGCGCAGCGGTTAACGCAAGCGCGGGGCGGCACGCCAAAAGGATAGCTGGGTGGCAGCcgggctggcc                                                                                                                                             >  2:338907/1‑151 (MQ=255)
               gCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTcc                                                                                                                              <  2:786839/151‑1 (MQ=255)
                cGGACAGCGGGATTATTTAGCGGCGGTTTGGGGGGGGTAAATGCCAGAAAGGCGGCGGTCATCCCAGGGCGCAGGGGTAAAAGCAAACGGGGGGCGTCACGCAAAATGGGTAGCTGGCTGGGAGCCGGGCCGGCCCCAAAGCCCCATTccc                                                                                                                             >  2:402166/1‑151 (MQ=255)
                                      gcggTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGAc                                                                                                        >  1:46964/1‑150 (MQ=255)
                                           ttGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCg                                                                                                   <  1:728961/150‑1 (MQ=255)
                                            tGGCGGTGTTAAATGCCAGGTAGGCGACGTTCATCCCAGGACGCAGCGATAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTTGCCAGAACGTCGCATTCCCCGGGCAGGAGTTTCGACAGACGTCCg                                                                                                   >  1:482409/1‑149 (MQ=255)
                                                ggTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgccg                                                                                             >  2:185484/1‑151 (MQ=255)
                                                   gtTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGccgccg                                                                                          >  2:634265/1‑151 (MQ=255)
                                                     tAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCgg                                                                                         >  1:146604/1‑150 (MQ=255)
                                                      aaaTGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCgg                                                                                         >  2:627796/1‑149 (MQ=255)
                                                       aaTGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGc                                                                                      <  1:402172/151‑1 (MQ=255)
                                                         agCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCt                                                                                    >  1:38827/2‑151 (MQ=255)
                                                                    cGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCcaccac                                                                         >  1:347876/1‑151 (MQ=255)
                                                                       cGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTAc                                                                      >  1:433212/1‑151 (MQ=255)
                                                                        gTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTAcc                                                                     >  1:741499/1‑151 (MQ=255)
                                                                              cccAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCgg                                                                <  2:75635/150‑1 (MQ=255)
                                                                                   gACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTa                                                           >  2:671176/1‑150 (MQ=255)
                                                                                    aCGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAAc                                                         >  1:106263/1‑151 (MQ=255)
                                                                                     cGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCCTTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACg                                                        >  1:447311/1‑151 (MQ=255)
                                                                                      gCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCACTAACgg                                                       <  1:696595/151‑1 (MQ=255)
                                                                                        aGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTCCCTGCGGCATTAACGGtt                                                     <  2:323980/151‑1 (MQ=255)
                                                                                        aGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGtt                                                     <  1:748394/151‑1 (MQ=255)
                                                                                             tAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTAcc                                                 <  2:607867/150‑1 (MQ=255)
                                                                                                       cgcgGGTCGTCACGCTAAATGGATAGCTTGCTGGCAGCAGGCCGGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTCCCTGCGGCATTAACGGTTTTCCGCGCATGAAg                                       <  1:85223/150‑1 (MQ=255)
                                                                                                        gcgGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGtc                                     <  2:495669/151‑1 (MQ=255)
                                                                                                        gcgGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGtc                                     >  1:738630/1‑151 (MQ=255)
                                                                                                         cgGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGtc                                     <  1:772587/150‑1 (MQ=255)
                                                                                                         cgGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGtc                                     >  1:389955/1‑150 (MQ=255)
                                                                                                           ggTCGTCGCTCATAATGGACAGCTGGCTGGCAGCAGGCCTGGCCAGAACTTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTTCGGTGCCGCCGGAGCCGAAATCCACCCCTCCCTGCGGCATTAACGGTTTACCGCGCCAGAAGtcat                                   <  1:186062/150‑1 (MQ=255)
                                                                                                                 cACGCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGAc                             <  2:13451/150‑1 (MQ=255)
                                                                                                                 cACGCAAAATGGATACCTGGATGGCAGCAGGCCTGGCCAGAATGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGTCGCCGGAGCCTAAATCCACCACAACCTGCGGCATTAACGGTTTACCGCGACAGAAGTCATCATGACg                            >  1:287714/1‑151 (MQ=255)
                                                                                                                    gCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTg                         <  2:43446/151‑1 (MQ=255)
                                                                                                                    gCAAAATGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTg                         <  1:468180/151‑1 (MQ=255)
                                                                                                                      aaaaTGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGt                        >  2:685939/1‑150 (MQ=255)
                                                                                                                          tGGATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCg                   >  2:564190/1‑151 (MQ=255)
                                                                                                                           ggATAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCg                   >  2:311549/1‑150 (MQ=255)
                                                                                                                             aTAGCTGGCTGGCAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAAt                <  1:406759/151‑1 (MQ=255)
                                                                                                                                   ggctggcAGCAGGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAAtt           <  2:189829/150‑1 (MQ=255)
                                                                                                                                           gcagGCCTGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGgcgc  >  2:717528/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCATTAACGGTTTACCGCGCCAGAAGTCATCATGACGTTGTAGGCGAATAAATTGCCCGGCGC  >  NC_000913/1356151‑1356440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑