Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,673	A→C	F139V (TTC→GTC)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,673	0	A	C	100.0%	206.1 / NA	55	F139V (TTC→GTC)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (32/23);  total (32/23)

GCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGC  >  NC_000913/3400526‑3400818                                                                                                                                                    |                                                                                                                                                  gcACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGgacgac                                                                                                                                                <  1:624569/151‑1 (MQ=255)      gaagaGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCAc                                                                                                                                           >  2:255314/1‑151 (MQ=255)        agagGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACgg                                                                                                                                         >  1:277736/1‑151 (MQ=255)        agagGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACgg                                                                                                                                         <  1:682012/151‑1 (MQ=255)         gaggagTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACggg                                                                                                                                        >  2:36892/1‑151 (MQ=255)            gagTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCa                                                                                                                                      >  1:239710/1‑150 (MQ=255)              gTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCa                                                                                                                                   <  1:30444/151‑1 (MQ=255)               tAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCAcc                                                                                                                                    >  2:429553/1‑149 (MQ=255)                aaaCCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAgc                                                                                                                                 >  1:190108/1‑151 (MQ=255)                         cGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTgcg                                                                                                                         >  1:648928/1‑150 (MQ=255)                         cGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTgc                                                                                                                          <  1:593419/149‑1 (MQ=255)                           ttCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCgg                                                                                                                      >  1:309275/1‑151 (MQ=255)                               aGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTc                                                                                                                  >  2:515660/1‑151 (MQ=255)                                ggAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTc                                                                                                                  >  2:353749/1‑150 (MQ=255)                                    aTAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGa                                                                                                              >  1:462141/1‑150 (MQ=255)                                         aGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgcg                                                                                                        <  2:273940/151‑1 (MQ=255)                                            aaCTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCCGAAACCGGCAGGCCAGCACCAATTGCGTCAGCCATCGGCTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCCCGGATTCACGAATTGCgcgg                                                                                                     >  1:216591/1‑151 (MQ=255)                                            aaCTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCgcgg                                                                                                     >  2:308607/1‑151 (MQ=255)                                                tCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGtt                                                                                                 >  2:380453/1‑151 (MQ=255)                                                tCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGt                                                                                                  <  2:87875/150‑1 (MQ=255)                                                 cAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTc                                                                                                >  2:533357/1‑151 (MQ=255)                                                  aGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCa                                                                                               <  1:3085/151‑1 (MQ=255)                                                        accaccaccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTg                                                                                          <  1:284574/150‑1 (MQ=255)                                                             caccGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTcgc                                                                                    <  2:35098/151‑1 (MQ=255)                                                              accGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTcgcg                                                                                   >  2:787963/1‑151 (MQ=255)                                                                   tatCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAAc                                                                              >  2:43468/1‑151 (MQ=255)                                                                        accaccTCAGCACCTGTCGTTTCAGAAACCAGCAGGCCAGCACCAATCACGGCAGCCATCGGTTCTTCAATCAGGACGATTTCACGGCCACCAGCCCCCTCCGCGGCTCCATGAACGGTGCGGCGCTCAACCTGGTCCGCGCCCACCCGc                                                                          >  1:186907/1‑150 (MQ=255)                                                                        accaccATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGca                                                                         <  1:37652/151‑1 (MQ=255)                                                                            ccaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGcacaca                                                                     <  2:491510/151‑1 (MQ=255)                                                                            ccaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGcacac                                                                      >  1:393397/1‑150 (MQ=255)                                                                              aTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACaaa                                                                   <  2:462123/151‑1 (MQ=255)                                                                                 gAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCa                                                                <  2:48947/151‑1 (MQ=255)                                                                                      ggTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAc                                                            >  2:756048/1‑150 (MQ=255)                                                                                            ttCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCggg                                                     >  2:796138/1‑151 (MQ=255)                                                                                             tCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCggg                                                     >  1:701899/1‑150 (MQ=255)                                                                                                gAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTg                                                 <  2:596936/151‑1 (MQ=255)                                                                                                gAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTg                                                 >  1:263492/1‑151 (MQ=255)                                                                                                 aaaCCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTgg                                                <  1:43471/151‑1 (MQ=255)                                                                                                 aaaCCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTgg                                                <  1:397295/151‑1 (MQ=255)                                                                                                    ccGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACg                                             >  1:422224/1‑151 (MQ=255)                                                                                                        cAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATa                                         <  1:761299/151‑1 (MQ=255)                                                                                                          ggCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATaaa                                       <  1:142845/151‑1 (MQ=255)                                                                                                           gCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAg                                      <  1:310839/151‑1 (MQ=255)                                                                                                               gCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTg                                   <  1:298977/150‑1 (MQ=255)                                                                                                                   cAATTGCGGCAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCt                              >  2:47147/1‑151 (MQ=255)                                                                                                                            cAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTg                     >  2:1447/1‑151 (MQ=255)                                                                                                                            cAGCCATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTg                     <  2:648870/151‑1 (MQ=255)                                                                                                                               ccATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGttt                  <  2:204646/151‑1 (MQ=255)                                                                                                                               ccATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGttt                  >  2:690303/1‑151 (MQ=255)                                                                                                                                cATCGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGCCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATTAAGCTGATGCTGTGCACTTGttt                  >  1:256468/1‑150 (MQ=255)                                                                                                                                   cGGTTCTTCAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtgat               <  1:159221/150‑1 (MQ=255)                                                                                                                                        cttcAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGAGCTTGGACGCATAAAGCTGTTGCTGTGGACATGTTTGATGAAGt          >  1:745972/1‑150 (MQ=255)                                                                                                                                          tcAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTg         <  1:547411/149‑1 (MQ=255)                                                                                                                                           cAATCAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTg      >  1:650263/1‑151 (MQ=255)                                                                                                                                               cAGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGc  >  1:629256/1‑151 (MQ=255)                                                                                                                                                aGGACGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAg   >  1:603046/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGC  >  NC_000913/3400526‑3400818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑