Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,574,0790CA42.2% 75.4 / 20.9 45G375V (GGT→GTT) pqqLputative periplasmic M16 family zinc metalloendopeptidase
Reads supporting (aligned to +/- strand):  ref base C (6/20);  new base A (19/0);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 7.46e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAGAAATAGTCTTCAAC  >  NC_000913/1573937‑1574228
                                                                                                                                              |                                                                                                                                                       
cAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTTGGTTTTCAATTATCGGGTTCTTCTACAGAAAAAACa                                                                                                                                                       >  2:232968/1‑145 (MQ=255)
  aGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTAAATTATCGGGTTTTTTAGCAGAAAAAACa                                                                                                                                                       >  1:451694/1‑143 (MQ=255)
  aGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTAAATCATCGAGTTCTTTTACAGAAAAAACAAGCTg                                                                                                                                                  >  1:671367/1‑148 (MQ=255)
     aCGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AAt                                                                                                                                           <  2:697427/151‑1 (MQ=255)
     aCGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTGACATCATCGCGGTTTTTCGCCGAAAAACCAAGCTGAt                                                                                                                                                >  2:486298/1‑147 (MQ=255)
       ggCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATgg                                                                                                                                         <  1:289495/151‑1 (MQ=255)
        gCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGt                                                                                                                                        <  2:159835/151‑1 (MQ=255)
          gTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGGGATTTTGCCTCCACCAGTTCTTTACCACAAAAAACACGCTGAAC‑AAttggt                                                                                                                                       >  2:186588/1‑146 (MQ=255)
              tattTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCa                                                                                                                                   <  2:58179/150‑1 (MQ=255)
                     tGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACCACATCGAGGTCTTTATCAGAAAAAACAATCTGATT‑AAAGGGTGCCAACACTGcc                                                                                                                           >  2:494035/1‑151 (MQ=255)
                     tGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGAATTTGCATCATCGGGGTTTTCAGCAGAAAAAACACGCTGATC‑AATTGTTGCCCACACTGcc                                                                                                                           >  2:191065/1‑151 (MQ=255)
                          tGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGAATTGACATCATCGAGTTTTTTAGCAGAAAAAACAAACTGATCAAATGGTGGCC‑ACTCTTCCA‑tt                                                                                                                       >  1:930013/1‑149 (MQ=255)
                           ggATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTaa                                                                                                                     <  1:578155/150‑1 (MQ=255)
                           ggATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAAt                                                                                                                    <  2:245872/151‑1 (MQ=255)
                              tGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTGTCATCAACCGGATTTTTTGCAGAAAAAACACGCCGATC‑AATTGTTTGCCACACTGCCC‑TTTATgt                                                                                                                  >  1:597081/1‑149 (MQ=255)
                               gCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCAt                                                                                                                <  2:316295/151‑1 (MQ=255)
                                ccAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGAATTGTAAACATCGAGGTTTTCCGCAGAAAAAACACGCTGATC‑AATGGGTGCCAACTCTGCCC‑TTAATgtctt                                                                                                               >  1:761059/1‑147 (MQ=255)
                                 cAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTATATTATCGAGGTATTCAGCCGAAAAAACAAGCCGATT‑AAAGGTTTCCAACTCTGCCC‑TTAATGAAtt                                                                                                               >  2:346086/1‑150 (MQ=255)
                                 cAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATTGAGGTCTTTAGCAGAAAAAACAAACTGGTT‑AAAGGGTGCCAAATCTTCCC‑TTTAttgatt                                                                                                               >  2:583265/1‑145 (MQ=255)
                                      gACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACAATATCGCGTTTTTTATCAGAAAAACCACGCTGATC‑AATGGTTGCCCACCCTGCCA‑TTTATGccatctcacc                                                                                                         >  2:59140/1‑142 (MQ=255)
                                       aCTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGCGTTCTTCAGCCGAAAAAACATGCTGATC‑AATGGTTGGCAACTCTGCCA‑TTTATGCATTTgcagc                                                                                                         >  2:288463/1‑150 (MQ=255)
                                       aCTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCgcagca                                                                                                        <  1:128961/151‑1 (MQ=255)
                                                    cGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCAtattat                                                                                           <  1:327202/151‑1 (MQ=255)
                                                             cGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTg                                                                                  <  2:527958/151‑1 (MQ=255)
                                                             cGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTg                                                                                  <  1:73647/151‑1 (MQ=255)
                                                                  aGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCAttt                                                                             <  1:968279/151‑1 (MQ=255)
                                                                    cTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGATATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCACCTCTGCCA‑TTAATGCATTCGCAGCTTCCTGCATATTATCGTCTCGTGCAttt                                                                             <  1:224889/149‑1 (MQ=255)
                                                                        cTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGAATTTACATCATCGAGGTCTTTAGCAGAAAAAACATGCTGATC‑AATTGTTGCCCACCCTTCCC‑TTTATTCATTTCCAGCATCCTGCATATTTTCGTCTCGTGCCTTTTCACgg                                                                       >  2:139755/1‑151 (MQ=255)
                                                                          gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGa                                                                      <  2:658954/150‑1 (MQ=255)
                                                                           aTCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATCGAGTTCTTCAGCAGAAAAAACAAGCTGATC‑AATGGGTGCCAACTCTTCCC‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGaa                                                                     >  2:500568/1‑150 (MQ=255)
                                                                             cAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGaaaaa                                                                  <  2:714105/151‑1 (MQ=255)
                                                                              aaCCGCATTTTTCAGCCAGGTGAGGCGGGGAGAATTTACATCATCCGGTTTTTTTGCAGAAAAAAAAAGCTGGTC‑AATGGGTGGCAAACCTGCCC‑TTTATTCAATCGCCGCCTCCTGCATAATTTTGTTTCCTGCATTTAAACGGgaaaa                                                                  >  1:128215/1‑150 (MQ=255)
                                                                               aCCGCATTTTTCAGCCAGGTGAGGCGGGTTGATTTTACATCCACCAGATTTTTTGCACAAAAACCAAGCTGATT‑AATGGGTGCCAAATCTTTCATTTATTGCTTTC‑CAGCACCCCTCATATTTTCGTTTCCTGCCTTTTAACGGgaaaa                                                                  >  1:260749/1‑149 (MQ=255)
                                                                                  gCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCCTCGAGGTTTTTCTCAGAAAAAACATGCTGATC‑AATGGGTTCCAACTCTGCCC‑TTTATGCATTCCCAGCATCCTGCATATTTTCGTCTCGTGCATTTTCACGGgaaaa                                                                  >  2:674586/1‑146 (MQ=255)
                                                                                   cATTTTTCAGCCAGGTGAGGCGGGTAGATTTTTCATCATCGAGGTTTTCAGCAGAAAAAACATGCTGATC‑AATGGTTGCCAACCCTTCCC‑TTAATGCATTCGCCGCATCCTGCATATTATTGTCTCGTGCATTTTCACGGaaaaaaa                                                                >  2:505422/1‑145 (MQ=255)
                                                                                    aTTTTTCAGCCAGGTGAGGCGGGGAGATTTTTCATCCTCGAGTTTTTTCACCGAAAAAACAAGCTGATC‑AAAGGTTGCCAACTCTGCCA‑TTTATGCATTTCCAACATCCTGCCTATTTTCGTCTCCTGCATTTTCACGGaaaaa                                                                  >  1:125596/1‑144 (MQ=255)
                                                                                    aTTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATCGAGTTCTTTTGCAGAAAAAACAAACTGATC‑AATGGTTGTCCACTCTGCCA‑TTAATGCATTCTCAGCATCCTGCATATTTTCGTCTCGTGCATTTTCACGGGAAAACAGCGa                                                            >  2:102292/1‑150 (MQ=255)
                                                                                             ccAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCg                                                  <  1:805548/151‑1 (MQ=255)
                                                                                                                ttGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTgc                               >  1:479494/1‑151 (MQ=255)
                                                                                                                   aCATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTgcgcg                            >  1:151055/1‑151 (MQ=255)
                                                                                                                                  tcAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAg                <  2:440093/148‑1 (MQ=255)
                                                                                                                                    agcagAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAGAAATa           <  1:148316/151‑1 (MQ=255)
                                                                                                                                      cagAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAGAAATAGt         <  2:671853/151‑1 (MQ=255)
                                                                                                                                         aaaaaCCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAGAAATAGTCt       <  2:105689/150‑1 (MQ=255)
                                                                                                                                             aCCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAGAAATAGTCTTCAAc  >  1:787336/1‑151 (MQ=255)
                                                                                                                                              |                                                                                                                                                       
CAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATC‑AATGGTTGCCAACTCTGCCA‑TTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAGAAATAGTCTTCAAC  >  NC_000913/1573937‑1574228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑