Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,574,0790CA42.2% 75.4 / 20.9 45G375V (GGT→GTT) pqqLputative periplasmic M16 family zinc metalloendopeptidase
Reads supporting (aligned to +/- strand):  ref base C (6/20);  new base A (19/0);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 7.46e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAGAAATAGTCTTCAAC  >  NC_000913/1573937‑1574228
                                                                                                                                              |                                                                                                                                                       
cAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTTGGTTTTCAATTATCGGGTTCTTCTACAGAAAAAACa                                                                                                                                                       >  2:232968/1‑145 (MQ=255)
  aGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTAAATTATCGGGTTTTTTAGCAGAAAAAACa                                                                                                                                                       >  1:451694/1‑143 (MQ=255)
  aGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTAAATCATCGAGTTCTTTTACAGAAAAAACAAGCTg                                                                                                                                                  >  1:671367/1‑148 (MQ=255)
     aCGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAAt                                                                                                                                           <  2:697427/151‑1 (MQ=255)
     aCGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTGACATCATCGCGGTTTTTCGCCGAAAAACCAAGCTGAt                                                                                                                                                >  2:486298/1‑147 (MQ=255)
       ggCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATgg                                                                                                                                         <  1:289495/151‑1 (MQ=255)
        gCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGt                                                                                                                                        <  2:159835/151‑1 (MQ=255)
          gTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGGGATTTTGCCTCCACCAGTTCTTTACCACAAAAAACACGCTGAACAAttggt                                                                                                                                       >  2:186588/1‑146 (MQ=255)
              tattTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCa                                                                                                                                   <  2:58179/150‑1 (MQ=255)
                     tGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACCACATCGAGGTCTTTATCAGAAAAAACAATCTGATTAAAGGGTGCCAACACTGcc                                                                                                                           >  2:494035/1‑151 (MQ=255)
                     tGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGAATTTGCATCATCGGGGTTTTCAGCAGAAAAAACACGCTGATCAATTGTTGCCCACACTGcc                                                                                                                           >  2:191065/1‑151 (MQ=255)
                          tGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGAATTGACATCATCGAGTTTTTTAGCAGAAAAAACAAACTGATCAAATGGTGGCCACTCTTCCAtt                                                                                                                       >  1:930013/1‑149 (MQ=255)
                           ggATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa                                                                                                                     <  1:578155/150‑1 (MQ=255)
                           ggATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAAt                                                                                                                    <  2:245872/151‑1 (MQ=255)
                              tGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTGTCATCAACCGGATTTTTTGCAGAAAAAACACGCCGATCAATTGTTTGCCACACTGCCCTTTATgt                                                                                                                  >  1:597081/1‑149 (MQ=255)
                               gCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCAt                                                                                                                <  2:316295/151‑1 (MQ=255)
                                ccAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGAATTGTAAACATCGAGGTTTTCCGCAGAAAAAACACGCTGATCAATGGGTGCCAACTCTGCCCTTAATgtctt                                                                                                               >  1:761059/1‑147 (MQ=255)
                                 cAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTATATTATCGAGGTATTCAGCCGAAAAAACAAGCCGATTAAAGGTTTCCAACTCTGCCCTTAATGAAtt                                                                                                               >  2:346086/1‑150 (MQ=255)
                                 cAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATTGAGGTCTTTAGCAGAAAAAACAAACTGGTTAAAGGGTGCCAAATCTTCCCTTTAttgatt                                                                                                               >  2:583265/1‑145 (MQ=255)
                                      gACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACAATATCGCGTTTTTTATCAGAAAAACCACGCTGATCAATGGTTGCCCACCCTGCCATTTATGccatctcacc                                                                                                         >  2:59140/1‑142 (MQ=255)
                                       aCTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGCGTTCTTCAGCCGAAAAAACATGCTGATCAATGGTTGGCAACTCTGCCATTTATGCATTTgcagc                                                                                                         >  2:288463/1‑150 (MQ=255)
                                       aCTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcagca                                                                                                        <  1:128961/151‑1 (MQ=255)
                                                    cGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCAtattat                                                                                           <  1:327202/151‑1 (MQ=255)
                                                             cGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTg                                                                                  <  2:527958/151‑1 (MQ=255)
                                                             cGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTg                                                                                  <  1:73647/151‑1 (MQ=255)
                                                                  aGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCAttt                                                                             <  1:968279/151‑1 (MQ=255)
                                                                    cTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGATATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCACCTCTGCCATTAATGCATTCGCAGCTTCCTGCATATTATCGTCTCGTGCAttt                                                                             <  1:224889/149‑1 (MQ=255)
                                                                        cTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGAATTTACATCATCGAGGTCTTTAGCAGAAAAAACATGCTGATCAATTGTTGCCCACCCTTCCCTTTATTCATTTCCAGCATCCTGCATATTTTCGTCTCGTGCCTTTTCACgg                                                                       >  2:139755/1‑151 (MQ=255)
                                                                          gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGa                                                                      <  2:658954/150‑1 (MQ=255)
                                                                           aTCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATCGAGTTCTTCAGCAGAAAAAACAAGCTGATCAATGGGTGCCAACTCTTCCCTTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGaa                                                                     >  2:500568/1‑150 (MQ=255)
                                                                             cAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGaaaaa                                                                  <  2:714105/151‑1 (MQ=255)
                                                                              aaCCGCATTTTTCAGCCAGGTGAGGCGGGGAGAATTTACATCATCCGGTTTTTTTGCAGAAAAAAAAAGCTGGTCAATGGGTGGCAAACCTGCCCTTTATTCAATCGCCGCCTCCTGCATAATTTTGTTTCCTGCATTTAAACGGgaaaa                                                                  >  1:128215/1‑150 (MQ=255)
                                                                               aCCGCATTTTTCAGCCAGGTGAGGCGGGTTGATTTTACATCCACCAGATTTTTTGCACAAAAACCAAGCTGATTAATGGGTGCCAAATCTTTCATTTATTGCTTTCCAGCACCCCTCATATTTTCGTTTCCTGCCTTTTAACGGgaaaa                                                                  >  1:260749/1‑149 (MQ=255)
                                                                                  gCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCCTCGAGGTTTTTCTCAGAAAAAACATGCTGATCAATGGGTTCCAACTCTGCCCTTTATGCATTCCCAGCATCCTGCATATTTTCGTCTCGTGCATTTTCACGGgaaaa                                                                  >  2:674586/1‑146 (MQ=255)
                                                                                   cATTTTTCAGCCAGGTGAGGCGGGTAGATTTTTCATCATCGAGGTTTTCAGCAGAAAAAACATGCTGATCAATGGTTGCCAACCCTTCCCTTAATGCATTCGCCGCATCCTGCATATTATTGTCTCGTGCATTTTCACGGaaaaaaa                                                                >  2:505422/1‑145 (MQ=255)
                                                                                    aTTTTTCAGCCAGGTGAGGCGGGGAGATTTTTCATCCTCGAGTTTTTTCACCGAAAAAACAAGCTGATCAAAGGTTGCCAACTCTGCCATTTATGCATTTCCAACATCCTGCCTATTTTCGTCTCCTGCATTTTCACGGaaaaa                                                                  >  1:125596/1‑144 (MQ=255)
                                                                                    aTTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATCGAGTTCTTTTGCAGAAAAAACAAACTGATCAATGGTTGTCCACTCTGCCATTAATGCATTCTCAGCATCCTGCATATTTTCGTCTCGTGCATTTTCACGGGAAAACAGCGa                                                            >  2:102292/1‑150 (MQ=255)
                                                                                             ccAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCg                                                  <  1:805548/151‑1 (MQ=255)
                                                                                                                ttGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTgc                               >  1:479494/1‑151 (MQ=255)
                                                                                                                   aCATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTgcgcg                            >  1:151055/1‑151 (MQ=255)
                                                                                                                                  tcAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAg                <  2:440093/148‑1 (MQ=255)
                                                                                                                                    agcagAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAGAAATa           <  1:148316/151‑1 (MQ=255)
                                                                                                                                      cagAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAGAAATAGt         <  2:671853/151‑1 (MQ=255)
                                                                                                                                         aaaaaCCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAGAAATAGTCt       <  2:105689/150‑1 (MQ=255)
                                                                                                                                             aCCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAGAAATAGTCTTCAAc  >  1:787336/1‑151 (MQ=255)
                                                                                                                                              |                                                                                                                                                       
CAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGCGCAGTGCCGCCAGAAATAGTCTTCAAC  >  NC_000913/1573937‑1574228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: