Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,581	0	G	T	44.2%	74.8 / 28.6	53	D133Y (GAT→TAT)	yegJ	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (7/22);  new base T (23/0);  total (31/22)
Fisher's exact test for biased strand distribution p-value = 8.45e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

TCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCC  >  NC_000913/2151434‑2151729                                                                                                                                                     |                                                                                                                                                      tCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGAt                                                                                                                                                     <  2:936788/150‑1 (MQ=255)    aaCGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATcc                                                                                                                                                   <  2:694566/149‑1 (MQ=255)          aTGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTgg                                                                                                                                           <  2:402934/151‑1 (MQ=255)          aTGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACATTTATCCttt                                                                                                                                                >  2:6553/1‑146 (MQ=255)                   ggCGTAATCAGCAATGGCCCTAGTATTGTTACAAACGTAGAATATGGGCAGGAGTGGAAAATCAAGAAAGAAGATATTACAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑TCCATTGATCCTTTATTGGTCACCTa                                                                                                                                    <  1:856222/149‑1 (MQ=255)                   ggCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTAt                                                                                                                                   <  1:216328/150‑1 (MQ=255)                    gCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑TTCATTTATCCTTTTTTTGTcccc                                                                                                                                      >  1:690784/1‑146 (MQ=255)                    gCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACAATTATACTTTTTTTGTCACCTAt                                                                                                                                   >  2:217831/1‑149 (MQ=255)                         aTCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑ACCATTTATTCTTTTTTTGTCACCTATCCcaaa                                                                                                                             >  2:798136/1‑150 (MQ=255)                         aTCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACATTTATTCTTTTTTTGTTACCTATTCcaaa                                                                                                                             >  1:1035224/1‑150 (MQ=255)                              cAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑AACATTTATTCTTTTTTTTTTACCCATCCAaaaaaa                                                                                                                          >  1:922077/1‑148 (MQ=255)                               aaTGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTTATCCTTTTTTTGTTACCTATCCaaaaaaaaaa                                                                                                                       >  2:434768/1‑150 (MQ=255)                                aTGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACCTTTATTCTTTTTTTGTCACCCATCCTAaaaaaaaa                                                                                                                       >  2:161181/1‑149 (MQ=255)                                aTGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGATATAACCATTATACCTTTTTTGTTAACC‑ATCCaaaaaaaaaa                                                                                                                       >  2:225487/1‑149 (MQ=255)                                  gACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACATTGATTCTTTTTTTGTTCCCTATCCAAAAAAAAAAGcc                                                                                                                    >  2:745710/1‑150 (MQ=255)                                         gTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑TAAAATTATTCTTTTTTTTTTACCTATTAAAAAAAAAAAGGcgacga                                                                                                               >  1:579230/1‑148 (MQ=255)                                             tGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTTATTCTTTTTTTGTTACCTATCCCAAAAAAAAAACcgacga                                                                                                               >  2:418014/1‑144 (MQ=255)                                              gTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAaga                                                                                                        <  1:311810/150‑1 (MQ=255)                                                tACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGc                                                                                                      <  2:701385/150‑1 (MQ=255)                                                 aCAAACGTGGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCaa                                                                                                    <  1:162801/151‑1 (MQ=255)                                                   aaaCGTAGAATATGGTCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑AACCTTTATTCCTTTTTTGTTACCCATCCAAAAAAAAAAAACGGCGAGCTAAAAGGaaa                                                                                                   >  2:433693/1‑150 (MQ=255)                                                    aaCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAt                                                                                                 <  1:777289/151‑1 (MQ=255)                                                    aaCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGGTGT‑ATCATTTATCATTTTTTGGTTACCCCTCCAAAAAAAGAAAAGGACGAACAAAGAGCaaaa                                                                                                  >  1:460422/1‑150 (MQ=255)                                                       gTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAg                                                                                              <  2:804576/151‑1 (MQ=255)                                                           aaTATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACATTTATCCTTTTTTTGTCACCTATCCAAAAAAAAAAACCCACGAGCCAAGAGCAAAAATAGtt                                                                                            >  2:1245/1‑149 (MQ=255)                                                            atatGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGATTT‑ATCAATGGTCCTTTTTTTGTTTACTAACCAAAAAAAAAAAGcgacgaaactagaacgaaa                                                                                                  >  1:428265/1‑129 (MQ=255)                                                                ggCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑ATCCTTTATCCTTTTTTTGTGTCCTTTTCCAAAAAAAAAACCGACGAGCTAAAAGCAAAAATAATTTGCtta                                                                                      >  2:754358/1‑148 (MQ=255)                                                                 gCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTTATCCTTTTTTTGTCACCCATCCAAAAAAAAAAGCCGACGAGCTAAAAGCAAAAATAGTTTGCTaa                                                                                      >  2:75365/1‑149 (MQ=255)                                                                   cAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑AACACTTTTTCTTTTTTTGTGACCCATCCAAAAAAAAAAAACGACGAGCTAAAAGGAAAAATATTTTGCTaa                                                                                      >  1:1041256/1‑147 (MQ=255)                                                                       aGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACCTTTATTCTTTTTTTGTTACCTATCCCAAAAAAAAAAACGACGAGCTAAAAGCAAAAATAATTTGCTTAtaaacc                                                                                >  1:834258/1‑144 (MQ=255)                                                                           gAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTtata                                                                          <  2:222473/151‑1 (MQ=255)                                                                            aaaaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTatat                                                                         <  1:222030/151‑1 (MQ=255)                                                                            aaaaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCAATTGTTCTTTTTTTGTAACCCATCCCAAAAAAAAAAACGACGAGGTAAAAGGAAAATTAGGTTGTTTATCAGc                                                                                 >  2:160142/1‑143 (MQ=255)                                                                              aaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTTATTCTTTTTTTGTTACCCATCCCAAAAAAGAAGACGACGAGCCAAAAGCAAAAATATTTTGCTAATCAGCCGGTAtattt                                                                       >  2:103918/1‑151 (MQ=255)                                                                                 cAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACATTTATTCTTTTTTTGTGACCCATCCCAAAAAAGAAAACGCCGAGCTAAGAGGAAAAATAATTTGCTAATTAACCGTTAtttttttt                                                                    >  2:622346/1‑151 (MQ=255)                                                                                     aaagaaGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑AACATTTATCCTTTTTTTGTTACCTATCCAAAAAAAAAAAACGACCAGCCAAGAGCAAAAATAATTCGCTAATCAGCCCGTTTAtttttttttt                                                                >  2:879961/1‑151 (MQ=255)                                                                                       agaagaTATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTaa                                                              <  2:360682/151‑1 (MQ=255)                                                                                                 tCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGt                                                     <  1:162800/150‑1 (MQ=255)                                                                                                  cAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGtt                                                    <  2:570518/150‑1 (MQ=255)                                                                                                  cAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGtt                                                    <  2:842382/150‑1 (MQ=255)                                                                                                    gACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑aa                                                <  2:3807/151‑1 (MQ=255)                                                                                                    gACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGGTTT‑AACAATTATTCTTTTTTTGTCACCCATACCAAAAAAAAAAACGACGAGCTAAAAGCAAAAATAATTTTCTAATCAACCCTTATATTTTTTTTTTTAAATTGCgg                                                      >  1:185069/1‑146 (MQ=255)                                                                                                     aCTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AAt                                               <  2:720105/151‑1 (MQ=255)                                                                                                          aTGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AAttgttg                                          <  1:732704/151‑1 (MQ=255)                                                                                                                aCGCGCGGGGATAAAATTTACGGCGGGTAT‑TCCACTTATCCTTTTTTGGGGACCTTTCCCAAAAAAGAAAACCACGAGGTAAAAGGAAAAATATTTCCGTAATTACACCTCTTATTTATTGTTTTAACTCGCGGTTgttatttttttgt                                       >  2:385317/1‑137 (MQ=255)                                                                                                                    gcgGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTCAAcct                                <  1:225400/151‑2 (MQ=255)                                                                                                                     cgGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTCAACCGc                               <  1:768127/151‑1 (MQ=255)                                                                                                                                            at‑aCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGGTG‑AATTGTTGTCTTCAAACGCCATGAGAAAATCAATCTGccc          >  1:164468/1‑149 (MQ=255)                                                                                                                                             t‑aCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑GATTGTTGTCTTTAAACGCCATGAGAAACTCACTCTGCCcac        >  2:666932/1‑150 (MQ=255)                                                                                                                                               aCCATTGATCCTTTATTGGACACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAATTGTT‑TCTTCAACCGACATTAGAAACTCACTTTGccccc        >  2:902768/1‑147 (MQ=255)                                                                                                                                                ccATTGTTCTTTTATTGGTCCCCTACCAAAAAAAAAACGCCGACGACCAAAGAGAAAAATTATTCCGCTAACCACCCTTTATTTTTATTGTTTAACCGGGCGTTTA‑AATTTTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCcac        <  1:217748/148‑1 (MQ=255)                                                                                                                                                 cATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCcaca       >  1:89832/1‑148 (MQ=255)                                                                                                                                                    tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTTAACCGCCATGGGAAACCCACTCTTCCCCCAGCGcc  >  1:40759/1‑150 (MQ=255)                                                                                                                                                     gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGcc  >  2:339461/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                      TCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCC  >  NC_000913/2151434‑2151729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑